FastQCFastQC Report
Wed 25 May 2016
SRR1780323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684212
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT201872.950401337597119No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC62710.9165287951687489No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG50040.7313522709335704No Hit
CACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTG40140.5866602748855617RNA PCR Primer, Index 36 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC11950.1746534699771416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTC250.002352848435.19766242
CTTTATA1800.034.224952
ATGCCGT30100.033.1805244
TCTTCTG5700.030.10326444
GTCTTCT5650.029.98030943
CGTCTTC5800.029.58424242
CCTTATA300.005704233429.374321
CCGTCTT5900.028.70995741
TCTTTAT2150.028.6911931
TATGCCG35500.028.4431843
AATTATC407.031722E-427.49817530
ATCGATA508.340004E-526.39824723
TTTATAC2750.024.8019773
CTCGCTA450.001396945324.44639213
GCCGTCT7450.023.03202640
AATCTCG43200.022.86422336
GTATGCC44300.022.84272642
GGACAAT44000.022.79848532
CTCGTAT43850.022.77613839
TCGTATG44550.022.7145440
Produced by FastQC (version 0.11.5)