FastQCFastQC Report
Wed 25 May 2016
SRR1780322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41092
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA150.002209610870.000011
GATGTGA150.002209610870.0000126
GAACGAG150.002209610870.0000131
GATTACG150.002209610870.0000119
GCCGACT308.237637E-658.33333620
ACAACTG200.00690461852.4999968
TTAATAC200.00690461852.4999962
GATCCAG200.00690461852.49999618
TGAACGA200.00690461852.49999630
GCCGGAG200.00690461852.49999613
TGGTGTA200.00690461852.49999636
AGGGTGT305.813037E-446.66666833
CATAAGG305.813037E-446.66666829
ACCGTGT404.5174667E-543.74999633
AACCGTG350.00124167940.032
GGGTGTA350.00124167940.034
CACGGCG350.00124167940.04
CCGTGTA350.00124167940.034
TTGAACC350.00124167940.029
CGATGTG350.00124167940.025
Produced by FastQC (version 0.11.5)