##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780322_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41092 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.571546773094518 23.0 12.0 33.0 12.0 34.0 2 22.91903533534508 23.0 12.0 34.0 12.0 34.0 3 22.90606444076706 23.0 11.0 34.0 11.0 34.0 4 22.698384113696097 23.0 11.0 34.0 11.0 34.0 5 22.978681981894287 23.0 11.0 34.0 11.0 34.0 6 23.548135890197607 24.0 11.0 36.0 11.0 38.0 7 24.36342840455563 26.0 12.0 36.0 11.0 38.0 8 23.74340504234401 26.0 11.0 36.0 11.0 38.0 9 24.41025017034946 26.0 12.0 36.0 11.0 38.0 10-11 24.129051883578313 26.0 11.0 36.0 11.0 38.0 12-13 24.317312372237904 26.0 11.0 36.0 11.0 38.0 14-15 23.71021123333009 25.5 11.0 36.0 11.0 38.0 16-17 23.61238197216003 24.5 11.0 36.0 11.0 38.0 18-19 23.80586245497907 25.5 11.0 36.0 11.0 38.0 20-21 23.712523118855252 24.5 11.0 35.5 11.0 38.0 22-23 23.67525309062591 24.5 11.0 36.0 11.0 38.0 24-25 23.532974788279958 23.5 11.0 36.0 11.0 38.0 26-27 23.303672247639444 23.0 11.0 35.0 11.0 38.0 28-29 23.18445196145235 23.0 11.0 35.0 11.0 38.0 30-31 23.219714299620364 23.0 11.0 35.0 11.0 38.0 32-33 23.19480677504137 23.0 11.0 35.5 11.0 38.0 34-35 23.262362503650344 23.0 11.0 35.0 11.0 38.0 36-37 23.27294850579188 23.5 11.0 34.5 11.0 38.0 38-39 23.105239462669132 22.5 11.0 34.0 11.0 38.0 40-41 23.130451182711965 23.0 11.0 35.0 11.0 38.0 42-43 23.354460722281708 24.0 11.0 35.0 11.0 38.0 44-45 23.540774359972744 24.5 11.0 36.0 11.0 38.0 46-47 23.133468801713228 22.5 11.0 35.5 11.0 38.0 48-49 22.917806385671177 22.0 11.0 34.5 11.0 38.0 50-51 22.91460624939161 22.0 11.0 34.5 11.0 38.0 52-53 23.161199260196632 23.0 11.0 34.5 11.0 38.0 54-55 23.094166747785458 23.0 11.0 35.0 11.0 38.0 56-57 22.97674729874428 22.5 11.0 35.0 11.0 38.0 58-59 22.960162562055874 22.5 11.0 34.5 11.0 38.0 60-61 23.02429913365132 23.0 11.0 35.0 11.0 38.0 62-63 22.810486226029397 22.5 11.0 34.0 11.0 38.0 64-65 22.676993088679062 22.5 11.0 34.0 11.0 38.0 66-67 22.714348291638274 23.0 11.0 34.0 11.0 38.0 68-69 22.588168013238587 22.5 11.0 34.0 11.0 38.0 70-71 22.229521561374476 21.0 11.0 34.0 11.0 38.0 72-73 21.81053489730361 20.0 11.0 34.0 11.0 38.0 74-75 21.848620169376034 20.0 11.0 34.0 11.0 38.0 76 21.015015088095005 19.0 11.0 31.0 11.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09099673159233745 1101 2 -0.43068652959138376 1101 3 -0.893618560725141 1101 4 -0.5695882678565631 1101 5 -0.5149768282906635 1101 6 -0.6137692946354214 1101 7 0.07800302789097202 1101 8 -0.5360032271386146 1101 9 -0.14774255734459274 1101 10-11 -0.5665289568395799 1101 12-13 -0.18679982675642748 1101 14-15 -0.8012958022480383 1101 16-17 -0.5197330572017158 1101 18-19 -0.8668448329711396 1101 20-21 0.19066855459453436 1101 22-23 -0.12434944736843079 1101 24-25 1.2260563226914556 1101 26-27 0.8211367787023924 1101 28-29 -0.3631660256718767 1101 30-31 -0.26915810583426136 1101 32-33 -0.7968590656856556 1101 34-35 -0.5878225908741541 1101 36-37 -0.25206407168582246 1101 38-39 0.8855887952343728 1101 40-41 -0.38625102515059595 1101 42-43 1.0193010863010983 1101 44-45 1.1705804316229305 1101 46-47 -0.39961155255282677 1101 48-49 -0.5140493667963462 1101 50-51 -0.11900379540285044 1101 52-53 0.21339644400735835 1101 54-55 -0.6756078484497188 1101 56-57 -0.8207554061372342 1101 58-59 0.5502222090053159 1101 60-61 0.6131603219883104 1101 62-63 0.2135412143187807 1101 64-65 0.30864718142112224 1101 66-67 -0.462610984552974 1101 68-69 -0.33445123512828445 1101 70-71 -0.22254107174190807 1101 72-73 -0.3825516347237645 1101 74-75 -0.8322961284796833 1101 76 -1.0434224844238926 1102 1 0.6840032684076611 1102 2 -0.9783055772104312 1102 3 -0.8608804654870461 1102 4 -0.6945882678565631 1102 5 -0.43402444733828105 1102 6 -2.4203169136830383 1102 7 -1.956520781632836 1102 8 -1.2675508461862321 1102 9 -1.9995282716303073 1102 10-11 -1.5831956235062457 1102 12-13 -2.290668874375477 1102 14-15 -1.8923672308194703 1102 16-17 -1.736697342915999 1102 18-19 -1.4427376901139972 1102 20-21 -0.8242123977864146 1102 22-23 -1.322266114035095 1102 24-25 -0.7730508201656896 1102 26-27 -2.126184649869039 1102 28-29 -2.3444160256718796 1102 30-31 -1.6504081058342628 1102 32-33 -0.5498352561618489 1102 34-35 -1.1765130670646293 1102 36-37 -1.5220045478762962 1102 38-39 -1.5382207285751512 1102 40-41 -1.0582748346744069 1102 42-43 -0.39409177084175795 1102 44-45 -1.2841814731389718 1102 46-47 -2.035921076362353 1102 48-49 -0.6077993667963462 1102 50-51 -0.929420462069519 1102 52-53 -1.1925559369450234 1102 54-55 -0.7654888008306706 1102 56-57 -1.2436720728039 1102 58-59 -1.7411468386137336 1102 60-61 -1.7740420589640706 1102 62-63 -1.0754468809193156 1102 64-65 -1.5196266281026887 1102 66-67 -1.005468127410115 1102 68-69 -1.9162964732235217 1102 70-71 -2.5686720241228613 1102 72-73 -1.2730278251999536 1102 74-75 -1.5468794618130168 1102 76 -1.1053272463286525 1103 1 1.8769236223899632 1103 2 2.8472843932910408 1103 3 1.4337360536869923 1103 4 1.621783413559367 1103 5 1.95926463820744 1103 6 1.465007570092773 1103 7 2.4362520797240954 1103 8 0.8768444340497545 1103 9 0.9029053566882759 1103 10-11 2.2155506891781194 1103 12-13 1.856381273117151 1103 14-15 1.1578540081185835 1103 16-17 0.62347964823444 1103 18-19 1.3631251417444084 1103 20-21 1.6942241803846763 1103 22-23 2.404135065905905 1103 24-25 1.0359093568254636 1103 26-27 2.0709392439362695 1103 28-29 1.9225087530891862 1103 30-31 2.309105168502022 1103 32-33 1.5921263957555567 1103 34-35 1.9695415052067595 1103 36-37 1.420620820855266 1103 38-39 1.6965875310118683 1103 40-41 0.6329493541161568 1103 42-43 1.279691414998947 1103 44-45 1.1268067274509974 1103 46-47 1.6796393956140498 1103 48-49 1.616205057982416 1103 50-51 0.4432196559245831 1103 52-53 0.6915886058278389 1103 54-55 1.4153371578713951 1103 56-57 1.1756494611194022 1103 58-59 0.5607705655160622 1103 60-61 0.10103168734861612 1103 62-63 1.1807109361898256 1103 64-65 1.0454913659976057 1103 66-67 2.064332757545639 1103 68-69 0.6602153261865062 1103 70-71 1.0130388903314156 1103 72-73 0.8493541806997484 1103 74-75 0.47882112815748457 1103 76 2.1408379454117608 1104 1 0.256225490629884 1104 2 0.8781759042710497 1104 3 0.9585639789573968 1104 4 0.5183746951063988 1104 5 0.7872718489580137 1104 6 0.852831234465107 1104 7 1.6249606998486428 1104 8 1.9768935982582114 1104 9 2.019916172814135 1104 10-11 1.4612488209381986 1104 12-13 1.4241459404393382 1104 14-15 1.3002253617731263 1104 16-17 1.385524879306221 1104 18-19 2.4345771247008194 1104 20-21 1.8748616762876544 1104 22-23 0.6082894415204585 1104 24-25 2.392226957612092 1104 26-27 1.294185720501332 1104 28-29 0.8069728632170126 1104 30-31 1.6579252274990743 1104 32-33 0.9084980771714868 1104 34-35 0.8248758218242607 1104 36-37 0.8770695261977757 1104 38-39 1.1488163084618854 1104 40-41 1.343577017177445 1104 42-43 1.0128526736026835 1104 44-45 1.36767037871288 1104 46-47 1.384449294008018 1104 48-49 1.491737670240692 1104 50-51 0.37520916756011147 1104 52-53 0.3139255445364597 1104 54-55 2.0780297176878477 1104 56-57 1.972068667936842 1104 58-59 1.9366309391640435 1104 60-61 1.2796616447396332 1104 62-63 1.3740901561177203 1104 64-65 1.423891890415831 1104 66-67 1.355180020738029 1104 68-69 1.5304627860357378 1104 70-71 1.6850316795808453 1104 72-73 1.396879582207454 1104 74-75 0.6850649826314275 1104 76 1.8150431240417184 1105 1 0.5684069381324335 1105 2 0.2736057377742753 1105 3 0.5561684641765616 1105 4 0.055411732143436865 1105 5 0.5238501703987204 1105 6 0.35223660313653227 1105 7 0.7895036831989657 1105 8 1.2075256064131565 1105 9 -0.699757629428472 1105 10-11 1.092874712885191 1105 12-13 -0.10550067865682067 1105 14-15 -0.14213787302130143 1105 16-17 -0.2541285355765517 1105 18-19 -0.004090901123170454 1105 20-21 -0.36626132745002593 1105 22-23 1.0072445893288169 1105 24-25 0.5422014734122946 1105 26-27 0.3607419556355502 1105 28-29 0.4226711302914232 1105 30-31 0.5745918941657386 1105 32-33 0.2869384441439635 1105 34-35 0.4172560460852175 1105 36-37 1.4035306203194189 1105 38-39 2.216519332586927 1105 40-41 1.384309263184921 1105 42-43 1.732399054846315 1105 44-45 0.7103903923044541 1105 46-47 0.106204305900647 1105 48-49 -0.12098744019084151 1105 50-51 -1.0877537954028504 1105 52-53 1.2124134820152221 1105 54-55 0.4039301594139779 1105 56-57 0.3418019333123077 1105 58-59 0.6091589717838204 1105 60-61 0.8710649746750718 1105 62-63 0.9211509784079013 1105 64-65 0.507819855077738 1105 66-67 0.3343254505715372 1105 68-69 0.42525551454405885 1105 70-71 1.8475359269474794 1105 72-73 0.8640899118031022 1105 74-75 0.39734836693315856 1105 76 0.7780060870046803 1106 1 2.0492206597120095 1106 2 1.519158190905511 1106 3 0.862670259150633 1106 4 0.6728030364912634 1106 5 -0.5430824183527747 1106 6 0.844538158780729 1106 7 1.4297111024251343 1106 8 1.7418694436688398 1106 9 0.6450369457609959 1106 10-11 2.6084710431604208 1106 12-13 1.657881850262207 1106 14-15 1.4217632039631418 1106 16-17 0.7198243962144311 1106 18-19 1.4762840490164386 1106 20-21 1.8054977471411213 1106 22-23 1.2900527265446122 1106 24-25 1.3524926580951835 1106 26-27 1.974902306652698 1106 28-29 1.0354752786759498 1106 30-31 1.761548415904869 1106 32-33 1.710671990214962 1106 34-35 2.607363744529575 1106 36-37 2.3743722637179054 1106 38-39 1.9632285467871675 1106 40-41 1.4312179189487821 1106 42-43 0.5999299682886736 1106 44-45 1.8024127297595811 1106 46-47 2.0948760250869256 1106 48-49 2.013396285377567 1106 50-51 1.8426809872058456 1106 52-53 1.4143281210259886 1106 54-55 1.7055256919229542 1106 56-57 1.5077772025584188 1106 58-59 1.5595777990674264 1106 60-61 1.3426246077025965 1106 62-63 0.9558936987908311 1106 64-65 0.5416052559552824 1106 66-67 1.055944916068146 1106 68-69 0.7498267151822731 1106 70-71 2.6110381208046753 1106 72-73 1.2228055081333764 1106 74-75 1.7543886541290128 1106 76 1.5693104348307685 1107 1 0.1361771814511421 1107 2 -0.6547548525727507 1107 3 0.5496267808897635 1107 4 -0.5011100069869983 1107 5 1.2482219294733135 1107 6 1.5662772892155097 1107 7 -0.8833323758357352 1107 8 0.2983911827992749 1107 9 -0.2940934890216127 1107 10-11 1.5823840866386796 1107 12-13 0.46657750243611673 1107 14-15 0.6261110300500938 1107 16-17 0.5589548309970418 1107 18-19 -0.41067247272269114 1107 20-21 0.8533238340976403 1107 22-23 0.5552701178489627 1107 24-25 0.9307535276604 1107 26-27 0.8096849153483525 1107 28-29 0.2789535395455154 1107 30-31 0.03111363329617589 1107 32-33 0.3932806858671398 1107 34-35 0.35518983148609706 1107 36-37 -0.3951929536733978 1107 38-39 0.0023589815697775407 1107 40-41 -0.3992168636599125 1107 42-43 0.5694951856799797 1107 44-45 0.9502388167161016 1107 46-47 0.547049938130403 1107 48-49 0.30470063320365526 1107 50-51 -0.28775379540284973 1107 52-53 0.08824116450425379 1107 54-55 -0.672735177642263 1107 56-57 0.5121250286453751 1107 58-59 0.05088214689351389 1107 60-61 0.3469724337895528 1107 62-63 -0.32671499686134453 1107 64-65 0.3676922124770208 1107 66-67 0.5211623073724958 1107 68-69 -0.05017328481772765 1107 70-71 0.06321203384815277 1107 72-73 -0.26849883969271104 1107 74-75 0.4891712628246623 1107 76 -0.3785156521257562 1108 1 0.8231337031902726 1108 2 0.840897321340293 1108 3 0.010496346107156995 1108 4 -0.7880665287261266 1108 5 -0.26482154878755537 1108 6 -1.5815487977410108 1108 7 0.012319798077307098 1108 8 0.32447813932101255 1108 9 0.8711239022827364 1108 10-11 0.39977539098650894 1108 12-13 0.014403589392639304 1108 14-15 0.021763203963139688 1108 16-17 -0.7540886472638242 1108 18-19 0.22845796205991675 1108 20-21 0.2924542688802525 1108 22-23 0.20309620480548318 1108 24-25 0.23944917983431324 1108 26-27 0.17925013273965718 1108 28-29 1.1528665830237763 1108 30-31 1.3224179811222605 1108 32-33 1.8280632945627886 1108 34-35 0.5769289619208777 1108 36-37 0.8135026985005105 1108 38-39 0.845837242439341 1108 40-41 2.1399135711226975 1108 42-43 0.4825386639408471 1108 44-45 0.793717077585665 1108 46-47 0.9513977642173579 1108 48-49 0.7003528071166976 1108 50-51 1.5557244654667137 1108 52-53 0.5099802949390337 1108 54-55 0.8620474310533872 1108 56-57 -0.0704836670068012 1108 58-59 -0.1752048096282266 1108 60-61 0.7904506946591177 1108 62-63 0.9385023944430024 1108 64-65 1.2024748211726717 1108 66-67 0.8515970899811869 1108 68-69 1.5585223673561863 1108 70-71 2.002342468630765 1108 72-73 1.0575881168290309 1108 74-75 0.20221474108553394 1108 76 -0.7785156521257548 1109 1 1.319401498496159 1109 2 0.11277111895476111 1109 3 1.4160369386427476 1109 4 1.444792263116888 1109 5 1.7380257001543455 1109 6 0.2526181895617974 1109 7 0.35660606202498 1109 8 1.2573754075010832 1109 9 0.6905159761573039 1109 10-11 0.312895821921483 1109 12-13 0.37408038816139566 1109 14-15 0.7905973709504401 1109 16-17 0.8491433650486009 1109 18-19 0.9914437258152056 1109 20-21 0.28714453436697696 1109 22-23 1.1474978977643104 1109 24-25 1.2659978524006839 1109 26-27 -0.09277757022302069 1109 28-29 0.38268574423962676 1109 30-31 0.02149454903299386 1109 32-33 0.08327683823343435 1109 34-35 1.4429928326403854 1109 36-37 0.04451462616500024 1109 38-39 0.395702575259655 1109 40-41 0.3895865222577477 1109 42-43 1.3593374326980658 1109 44-45 0.7197270814332981 1109 46-47 0.055745590304315584 1109 48-49 -0.7599011367078496 1109 50-51 0.3635736382254713 1109 52-53 0.34203108370394375 1109 54-55 0.44188583043776575 1109 56-57 0.9676848593494931 1109 58-59 0.41917764516207967 1109 60-61 0.5656334572601196 1109 62-63 0.5700914671632802 1109 64-65 0.5897391536082246 1109 66-67 0.874067270819971 1109 68-69 0.5141976270714608 1109 70-71 0.36702119121637367 1109 72-73 0.37147807450505965 1109 74-75 0.20006006621058248 1109 76 -0.08925055016345951 1110 1 0.8540967263515888 1110 2 1.3538595318238364 1110 3 0.43390146597712587 1110 4 0.5413930405546523 1110 5 0.7191686990791624 1110 6 0.185212681332537 1110 7 0.1676225205478481 1110 8 0.816717066586353 1110 9 1.1736025694911874 1110 10-11 -0.49059437740032763 1110 12-13 -0.0549990924440138 1110 14-15 0.6527262271244574 1110 16-17 0.3315269561494212 1110 18-19 -0.025097503198107773 1110 20-21 0.8728281006559513 1110 22-23 -0.4196181389572189 1110 24-25 1.2394491798343132 1110 26-27 0.8712452566730171 1110 28-29 0.5208877126458802 1110 30-31 0.6867414268760221 1110 32-33 0.6725167687602713 1110 34-35 -0.42810964027335174 1110 36-37 1.8594596265785306 1110 38-39 0.5518104240728263 1110 40-41 -0.04838386894231661 1110 42-43 -0.38369457458007616 1110 44-45 0.3456471872971676 1110 46-47 0.032381104323008 1110 48-49 0.24208381077374597 1110 50-51 0.44495648497098017 1110 52-53 -0.2976182422409721 1110 54-55 0.9376264639668364 1110 56-57 0.40383571535809537 1110 58-59 -0.21575743051404217 1110 60-61 -0.7713940838861895 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-0.17737483849446178 2116 50-51 0.24715186497450858 2116 52-53 0.1028843146273033 2116 54-55 -0.7431617568055096 2116 56-57 0.2541266693344646 2116 58-59 -1.220077656223797 2116 60-61 -0.012092373429481995 2116 62-63 -1.2604311576488776 2116 64-65 -0.8855071312473441 2116 66-67 -1.2340058632591742 2116 68-69 -1.3758090518398767 2116 70-71 -0.9180430933052506 2116 72-73 -0.7243643031873788 2116 74-75 -0.5817065058381736 2116 76 -0.8540693846934317 2117 1 0.3881699350743304 2117 2 0.10502775612290094 2117 3 0.597452867846286 2117 4 1.138745065476769 2117 5 0.02222555266171966 2117 6 1.183849752983626 2117 7 1.7997292183671618 2117 8 2.3345324871471007 2117 9 -0.23702827163030804 2117 10-11 -0.29569562350624423 2117 12-13 0.24474779229119292 2117 14-15 -0.021533897486136766 2117 16-17 0.8299693237506638 2117 18-19 -1.057321023447333 2117 20-21 1.2070376022135818 2117 22-23 0.5006505526315692 2117 24-25 -1.333467486832351 2117 26-27 0.02277368346429398 2117 28-29 0.8972506409947911 2117 30-31 0.20480022749907434 2117 32-33 1.226206410504819 2117 34-35 1.4099452662687035 2117 36-37 1.1623704521236995 2117 38-39 -0.4194707285751491 2117 40-41 -0.5291081680077383 2117 42-43 0.12049156249157633 2117 44-45 0.45331852686102536 2117 46-47 -0.17342107636234871 2117 48-49 1.0224089665369895 2117 50-51 0.6934962045971496 2117 52-53 0.920985729721643 2117 54-55 -0.3102804674973356 2117 56-57 1.0282029271961015 2117 58-59 -0.39843850528039937 2117 60-61 1.4353329410359308 2117 62-63 0.8683031190806823 2117 64-65 0.6605817052306406 2117 66-67 -0.19921812741011635 2117 68-69 0.43266186010981045 2117 70-71 0.5125779758771394 2117 72-73 1.0571805081333778 2117 74-75 0.990620538186981 2117 76 0.5592560870046803 2118 1 -1.3826633982590053 2118 2 0.16184593794108437 2118 3 0.34997812037153864 2118 4 0.7624824392141427 2118 5 -0.49134767966151216 2118 6 0.9546830863169617 2118 7 -0.6519753270873814 2118 8 -0.20744983608522105 2118 9 -0.28311413021616616 2118 10-11 -0.812741078051701 2118 12-13 -1.6861234198300217 2118 14-15 -0.34160965506189456 2118 16-17 0.4057268995082417 2118 18-19 -0.6277124375887446 2118 20-21 -0.5691618927359094 2118 22-23 -0.03691257868156228 2118 24-25 -0.3211568807717491 2118 26-27 -0.11280081148520082 2118 28-29 0.029195085439237545 2118 30-31 0.19075351032735455 2118 32-33 -0.5536231349497243 2118 34-35 1.1215929935414302 2118 36-37 -1.3851358610076083 2118 38-39 -0.4917560821105056 2118 40-41 -0.368123319522887 2118 42-43 -0.5161877304377178 2118 44-45 -0.4829188468763448 2118 46-47 -0.3684968339381065 2118 48-49 -0.37610744760442927 2118 50-51 -0.6786628863119404 2118 52-53 0.43092891153982293 2118 54-55 0.0405718052299342 2118 56-57 -0.5300357091675387 2118 58-59 0.40380265633575974 2118 60-61 -0.378587513509526 2118 62-63 0.30169958372714945 2118 64-65 -0.6956367291127918 2118 66-67 0.21839550895352033 2118 68-69 0.2194358500087965 2118 70-71 -0.006045761496597635 2118 72-73 0.8480580333859038 2118 74-75 0.44516599273243784 2118 76 -1.060377751379157 2119 1 0.744677425711032 2119 2 0.5081176437633488 2119 3 0.3558798341384204 2119 4 1.7632769006827615 2119 5 0.9394774253208951 2119 6 0.6138591162795066 2119 7 0.8410447614383223 2119 8 1.3180296781583358 2119 9 -0.13239344016963273 2119 10-11 1.5219541892278379 2119 12-13 1.5465970432275213 2119 14-15 1.6051046792928894 2119 16-17 0.8206060278705145 2119 18-19 1.5972903997736445 2119 20-21 1.075600336295981 2119 22-23 2.6730522380248303 2119 24-25 2.2113592921938654 2119 26-27 0.9083659119287155 2119 28-29 1.6877468956764368 2119 30-31 -0.05462158898032854 2119 32-33 -0.4145356306936847 2119 34-35 0.9392640864934201 2119 36-37 1.823126538266024 2119 38-39 0.9947380729229778 2119 40-41 0.8508075623293401 2119 42-43 1.1849812628661063 2119 44-45 0.9994327590707641 2119 46-47 1.1287792981694835 2119 48-49 0.5923410826418554 2119 50-51 0.9515720472937765 2119 52-53 0.7828185948901805 2119 54-55 0.7416273040382428 2119 56-57 0.48085976240209405 2119 58-59 1.3489280677533095 2119 60-61 1.781950450399222 2119 62-63 1.835180459904656 2119 64-65 1.88664678013701 2119 66-67 1.1550655804550551 2119 68-69 0.4505106428813441 2119 70-71 1.2743991369258296 2119 72-73 1.4688729238637137 2119 74-75 1.5879988153405407 2119 76 0.6319386712743444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 51.0 13 538.0 14 1884.0 15 2971.0 16 3631.0 17 4240.0 18 4207.0 19 3751.0 20 2844.0 21 2014.0 22 1327.0 23 955.0 24 672.0 25 488.0 26 395.0 27 389.0 28 318.0 29 337.0 30 364.0 31 388.0 32 445.0 33 608.0 34 725.0 35 1049.0 36 1840.0 37 4660.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.166942470553877 20.23751581816412 12.669132677893508 46.926409033388495 2 17.049547357149812 18.244427139102502 32.09870534410591 32.60732015964178 3 10.663876180278399 28.70875109510367 27.89107368830916 32.73629903630877 4 14.063564684123431 24.78584639345858 22.615107563516013 38.535481358901976 5 11.199260196631949 30.52662318699503 32.75090041857296 25.523216197800057 6 26.6572568869853 27.032025698432783 17.463253187968462 28.84746422661345 7 18.15195171809598 31.139881242090915 16.898666407086537 33.80950063272656 8 15.241409520101238 26.84950842013044 27.64771731723937 30.261364742528958 9 17.932930984133165 19.818942859924075 18.972062688601188 43.27606346734157 10-11 16.6443589993186 22.824394042636037 28.69293293098413 31.838314027061227 12-13 15.788961355008274 18.907573250267692 28.23177260780687 37.07169278691716 14-15 19.50257957753334 20.811836853888835 25.10464323956001 34.580940329017814 16-17 22.188287115340156 20.4227151600696 22.69812491634523 34.69087280824502 18-19 19.354132191180764 18.570524676336024 23.774700671663584 38.300642460819624 20-21 19.2531392971868 18.7785943736007 25.284726954151658 36.68353937506084 22-23 23.26730263798306 18.991531198286772 21.38615788961355 36.35500827411662 24-25 20.005353905308883 23.795675504666416 19.330031758392856 36.86893883163185 26-27 19.091307310425385 24.052126934683148 23.29407183880074 33.56249391609072 28-29 20.48330575294461 20.93351503942373 27.186556994062105 31.396622213569554 30-31 20.389613550082743 20.08420130439015 27.833884941107755 31.69230020441935 32-33 18.064343424510852 22.803708751095105 27.12450111943931 32.00744670495473 34-35 17.325756838314028 23.54107855543658 25.80429280638567 33.32887179986372 36-37 18.68246860702813 19.02925143580259 27.894724033875207 34.39355592329407 38-39 17.241798890294948 17.2381485447289 33.32157110873163 32.19848145624453 40-41 16.83417696875304 18.061909860800153 32.5257957753334 32.5781173951134 42-43 13.168013238586587 18.56444076705928 31.884551737564486 36.382994256789644 44-45 12.663202852171374 16.819780246523003 35.573050387549735 34.94396651375589 46-47 13.562250559719654 20.74369706998929 33.85817190694053 31.83588046335053 48-49 14.04166261072715 21.24379441253772 31.664314221746327 33.0502287549888 50-51 14.64396962912489 22.985009247542102 27.342305071546775 35.02871605178623 52-53 11.845371361822252 23.623819721600313 28.24637399007106 36.28443492650638 54-55 12.373454687043708 20.832522145429767 28.97644310328044 37.81758006424608 56-57 14.550277426263019 22.00063272656478 27.30215127032026 36.146938576851944 58-59 14.450501314124404 22.353499464615982 25.94178915604011 37.2542100652195 60-61 13.265355787014505 19.801907913949186 27.718290664849604 39.2144456341867 62-63 14.506473279470455 20.777766961939065 25.883383626983353 38.83237613160713 64-65 15.001703494597487 20.796018689769298 24.76516110191765 39.43711671371556 66-67 14.78511632434537 25.03772023751582 20.302005256497615 39.8751581816412 68-69 15.540737856517083 26.910347512897886 19.76418767643337 37.78472695415166 70-71 16.751435802589313 26.026963885914533 22.554268470748564 34.667331840747586 72-73 21.291248904896328 24.01562347902268 22.240338752068528 32.45278886401246 74-75 18.199406210454587 28.187968461014307 21.773094519614524 31.839530808916578 76 15.321717122554269 28.027353256108245 22.73921931276161 33.91171030857588 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 2.0 12 2.0 13 2.0 14 3.5 15 5.5 16 7.0 17 8.0 18 9.0 19 12.0 20 16.0 21 18.0 22 23.0 23 39.5 24 60.0 25 69.0 26 80.0 27 109.0 28 149.5 29 172.0 30 210.0 31 284.0 32 382.5 33 445.0 34 512.0 35 696.5 36 858.0 37 902.0 38 1005.5 39 1215.5 40 1441.5 41 1587.0 42 1613.0 43 1716.0 44 1897.0 45 1954.5 46 1934.0 47 1974.0 48 1901.0 49 1770.0 50 1752.0 51 1665.0 52 1533.0 53 1393.0 54 1298.0 55 1270.5 56 1201.0 57 1181.0 58 1203.0 59 1174.0 60 1197.5 61 1217.5 62 1185.0 63 1211.0 64 1194.0 65 1121.0 66 1013.5 67 936.0 68 896.5 69 843.5 70 750.0 71 670.0 72 620.5 73 532.5 74 401.0 75 308.0 76 278.0 77 183.5 78 93.5 79 68.0 80 53.0 81 29.0 82 15.5 83 11.0 84 10.0 85 11.0 86 10.0 87 7.0 88 6.0 89 7.0 90 7.0 91 4.0 92 3.0 93 5.5 94 6.5 95 4.0 96 3.0 97 2.5 98 3.0 99 12.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.001216781855348973 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001216781855348973 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.001216781855348973 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 41092.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.33203543268763 #Duplication Level Percentage of deduplicated Percentage of total 1 94.79818523153942 88.47707582984523 2 3.8068418856904462 7.106006035238003 3 1.0742594910304548 3.0078847464226612 4 0.22423863162286192 0.8371459164800934 5 0.07040050062578222 0.3285311009442227 6 0.005214851898206091 0.02920276452837535 7 0.0026074259491030453 0.017034945974885623 8 0.005214851898206091 0.038937019371167135 9 0.005214851898206091 0.04380414679256303 >10 0.007822277847309137 0.11437749440280348 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007300691132093838 0.0 2 0.0 0.0 0.0 0.017034945974885623 0.0 3 0.0 0.0 0.0 0.017034945974885623 0.0 4 0.0 0.0 0.0 0.04867127421395892 0.0 5 0.0 0.0 0.0 0.06083909276744865 0.0 6 0.0 0.0 0.0 0.06083909276744865 0.0 7 0.0 0.0 0.0 0.06570622018884455 0.0 8 0.0 0.0 0.0 0.06813978389954249 0.0 9 0.0 0.0 0.0 0.09004185729582401 0.0 10 0.0 0.0 0.0 0.10464323956001167 0.0 11 0.0 0.0 0.0 0.11681105811350141 0.0 12 0.0 0.0 0.0 0.11681105811350141 0.0 13 0.0 0.0 0.0 0.12897887666699115 0.0 14 0.0 0.0 0.0 0.12897887666699115 0.0 15 0.0 0.0 0.0 0.13384600408838704 0.0 16 0.0 0.0 0.0 0.13384600408838704 0.0 17 0.0 0.0 0.0 0.1533145137739706 0.0 18 0.0 0.0 0.0 0.16061520490606443 0.0 19 0.0 0.0 0.0 0.2117200428307213 0.0 20 0.0 0.0 0.0 0.23605567993770077 0.0 21 0.0 0.0 0.0 0.2482234984911905 0.0 22 0.0 0.0 0.0 0.2603913170446802 0.0 23 0.0 0.0 0.0 0.270125571887472 0.0 24 0.0 0.0 0.0 0.28229339044096174 0.0 25 0.0 0.0 0.0 0.30906259125863916 0.0 26 0.0 0.0 0.0 0.31879684610143094 0.0 27 0.0 0.0 0.0 0.39910444855446314 0.0 28 0.0 0.0 0.0 0.4915798695609851 0.0 29 0.0 0.0 0.0 0.5670203445926214 0.0 30 0.0 0.0 0.0 0.6473279470456537 0.0 31 0.0 0.0 0.0 0.7982088971089263 0.0 32 0.0 0.0 0.0 0.9636912294363866 0.0 33 0.0 0.0 0.0 1.2070476005061812 0.0 34 0.0 0.002433563710697946 0.0 1.5209773191862164 0.0 35 0.0 0.002433563710697946 0.0 1.939550277426263 0.0 36 0.0 0.002433563710697946 0.0 2.5309062591258638 0.0 37 0.0 0.004867127421395892 0.0 3.4434926506375936 0.0 38 0.0 0.004867127421395892 0.0 4.151659690450696 0.0 39 0.0 0.004867127421395892 0.0 4.730847853596807 0.0 40 0.0 0.004867127421395892 0.0 5.348973036114085 0.0 41 0.0 0.004867127421395892 0.0 6.001168110581135 0.0 42 0.0 0.004867127421395892 0.0 6.565754891463058 0.0 43 0.0 0.004867127421395892 0.0 7.032999123917064 0.0 44 0.0 0.004867127421395892 0.0 7.626788669327363 0.0 45 0.0 0.004867127421395892 0.0 8.142704175995327 0.0 46 0.0 0.004867127421395892 0.0 8.721892339141439 0.0 47 0.0 0.004867127421395892 0.0 9.245108536941498 0.0 48 0.0 0.004867127421395892 0.0 9.775625425873649 0.0 49 0.0 0.004867127421395892 0.0 10.206366202667185 0.0 50 0.0 0.004867127421395892 0.0 10.637106979460722 0.0 51 0.0 0.004867127421395892 0.0 11.021610045750998 0.0 52 0.0 0.004867127421395892 0.0 11.323371945877543 0.0 53 0.0 0.004867127421395892 0.0 11.690840066192933 0.0 54 0.0 0.004867127421395892 0.0 12.00233622116227 0.0 55 0.0 0.004867127421395892 0.0 12.316265939842305 0.0 56 0.0 0.004867127421395892 0.0 12.591258639151173 0.0 57 0.0 0.004867127421395892 0.0 12.914922612674 0.0 58 0.0 0.004867127421395892 0.0 13.28239073298939 0.0 59 0.0 0.004867127421395892 0.0 13.64985885330478 0.0 60 0.0 0.004867127421395892 0.0 13.93458580745644 0.0 61 0.0 0.004867127421395892 0.0 14.175508614815536 0.0 62 0.0 0.004867127421395892 0.0 14.413997858463935 0.0 63 0.0 0.004867127421395892 0.0 14.650053538401636 0.0 64 0.0 0.004867127421395892 0.0 14.934780492553296 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAATA 15 0.0022096108 70.00001 1 GATGTGA 15 0.0022096108 70.00001 26 GAACGAG 15 0.0022096108 70.00001 31 GATTACG 15 0.0022096108 70.00001 19 GCCGACT 30 8.237637E-6 58.333336 20 ACAACTG 20 0.006904618 52.499996 8 TTAATAC 20 0.006904618 52.499996 2 GATCCAG 20 0.006904618 52.499996 18 TGAACGA 20 0.006904618 52.499996 30 GCCGGAG 20 0.006904618 52.499996 13 TGGTGTA 20 0.006904618 52.499996 36 AGGGTGT 30 5.813037E-4 46.666668 33 CATAAGG 30 5.813037E-4 46.666668 29 ACCGTGT 40 4.5174667E-5 43.749996 33 AACCGTG 35 0.001241679 40.0 32 GGGTGTA 35 0.001241679 40.0 34 CACGGCG 35 0.001241679 40.0 4 CCGTGTA 35 0.001241679 40.0 34 TTGAACC 35 0.001241679 40.0 29 CGATGTG 35 0.001241679 40.0 25 >>END_MODULE