SRR1780322_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780322_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41092
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	14118	34.3570524676336	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	3556	8.653752555241896	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	3131	7.619487978195269	No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG	2679	6.519517180959798	RNA PCR Primer, Index 32 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	866	2.107466173464421	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	549	1.3360264771731725	RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	478	1.1632434537136183	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	268	0.6521950744670496	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	200	0.4867127421395892	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	189	0.45994354132191184	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	181	0.4404750316363283	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	169	0.4112722671079529	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	152	0.3699016840260878	RNA PCR Primer, Index 32 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	145	0.35286673805120217	RNA PCR Primer, Index 42 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG	134	0.3260975372335248	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC	116	0.28229339044096174	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	108	0.2628248807553782	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT	98	0.23848924364839869	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	98	0.23848924364839869	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGC	90	0.21902073396281516	No Hit
ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT	80	0.19468509685583568	RNA PCR Primer, Index 42 (95% over 24bp)
CTTATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	77	0.18738440572374185	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC	70	0.1703494597488562	No Hit
CTCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	60	0.14601382264187676	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG	56	0.13627956779908498	No Hit
CACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTTCTG	52	0.1265453129562932	RNA PCR Primer, Index 42 (95% over 23bp)
TCCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	51	0.12411174924559525	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG	46	0.11194393069210552	No Hit
CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	43	0.10464323956001167	No Hit
CACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTTCTG	42	0.10220967584931373	RNA PCR Primer, Index 42 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC	42	0.10220967584931373	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATATA	15	0.0022096108	70.00001	2
CTTATAT	15	0.0022096108	70.00001	1
TCTTTAT	120	0.0	70.00001	1
GCTCACC	15	0.0022096108	70.00001	15
ATATACA	15	0.0022096108	70.00001	4
AAAAAGG	20	7.838043E-5	69.99999	68
CTTTATA	130	0.0	64.61539	2
TTTATAC	135	0.0	62.22222	3
ACTCGAG	25	2.3638854E-4	55.999996	32
TCGAAAA	25	2.3638854E-4	55.999996	58
TCTGCTC	25	2.3638854E-4	55.999996	53
TATATAC	20	0.006904618	52.499996	3
TCTATAC	20	0.006904618	52.499996	1
CGAGGAA	20	0.006904618	52.499996	24
TCTGCCT	20	0.006904618	52.499996	53
CCTTATA	20	0.006904618	52.499996	1
CTGCCTG	20	0.006904618	52.499996	54
ACGCGCG	20	0.006904618	52.499996	38
AAACTAA	50	6.0073944E-8	48.999996	70
CTCGAAA	30	5.813037E-4	46.666668	57

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content