##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780322_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41092 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.68957461306337 34.0 34.0 34.0 34.0 34.0 2 33.680546091696684 34.0 34.0 34.0 34.0 34.0 3 33.69251922515331 34.0 34.0 34.0 34.0 34.0 4 33.73189428599241 34.0 34.0 34.0 34.0 34.0 5 33.71994548817288 34.0 34.0 34.0 34.0 34.0 6 37.51095103669814 38.0 38.0 38.0 37.0 38.0 7 37.48870826438236 38.0 38.0 38.0 37.0 38.0 8 37.444855446315586 38.0 38.0 38.0 37.0 38.0 9 37.47649177455466 38.0 38.0 38.0 37.0 38.0 10-11 37.390745157208215 38.0 38.0 38.0 37.0 38.0 12-13 37.33277766961939 38.0 38.0 38.0 37.0 38.0 14-15 37.23715078360752 38.0 38.0 38.0 36.0 38.0 16-17 37.19947921736591 38.0 38.0 38.0 36.0 38.0 18-19 37.033035627372726 38.0 38.0 38.0 35.0 38.0 20-21 37.13036600798209 38.0 38.0 38.0 35.5 38.0 22-23 37.225287160517865 38.0 38.0 38.0 36.0 38.0 24-25 37.1314611116519 38.0 38.0 38.0 35.5 38.0 26-27 36.93990314416431 38.0 37.5 38.0 35.0 38.0 28-29 36.81615642947533 38.0 37.0 38.0 34.5 38.0 30-31 36.81575489146306 38.0 37.0 38.0 34.5 38.0 32-33 36.36278351017229 38.0 37.0 38.0 34.0 38.0 34-35 36.50613258055096 38.0 37.0 38.0 34.0 38.0 36-37 36.51413900515915 38.0 37.0 38.0 34.0 38.0 38-39 32.91216051786236 37.0 33.5 38.0 21.0 38.0 40-41 34.84739121970213 37.0 35.0 38.0 28.5 38.0 42-43 36.55414679256303 38.0 37.0 38.0 34.0 38.0 44-45 36.85291540932542 38.0 37.0 38.0 34.5 38.0 46-47 36.76407816606638 38.0 37.0 38.0 34.5 38.0 48-49 36.789618417210164 38.0 37.0 38.0 34.5 38.0 50-51 36.34462912489049 38.0 37.0 38.0 34.0 38.0 52-53 36.24778545702327 38.0 37.0 38.0 34.0 38.0 54-55 36.27522388786139 38.0 37.0 38.0 34.0 38.0 56-57 36.334091794023166 38.0 37.0 38.0 34.0 38.0 58-59 35.93959894870048 38.0 37.0 38.0 32.0 38.0 60-61 35.85924267497323 38.0 37.0 38.0 31.5 38.0 62-63 35.97978925338265 38.0 37.0 38.0 32.0 38.0 64-65 35.62594908984717 38.0 36.5 38.0 30.5 38.0 66-67 34.536466952204805 38.0 35.0 38.0 24.0 38.0 68-69 33.8830064246082 38.0 34.0 38.0 22.0 38.0 70-71 30.55817434050423 34.5 27.0 38.0 15.5 38.0 72-73 24.630560693078944 24.0 11.0 36.5 10.0 38.0 74-75 23.127664752263215 23.0 11.0 35.0 11.0 38.0 76 22.91708848437652 23.0 11.0 34.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10275088007275457 1101 2 -0.1971084247487056 1101 3 -0.1588015964268905 1101 4 -0.04751903293196591 1101 5 0.0826829412615524 1101 6 -0.18422953988574875 1101 7 -0.29135609494821324 1101 8 -0.28863472463292084 1101 9 -0.18513947564511568 1101 10-11 0.04852909218340784 1101 12-13 -0.08205235721543147 1101 14-15 0.37451694782603084 1101 16-17 0.24109224653983574 1101 18-19 0.06828266366184721 1101 20-21 -0.02664965555402432 1101 22-23 -0.03635275300142382 1101 24-25 -0.3039059910799793 1101 26-27 9.282568727329021E-4 1101 28-29 0.2595040647203106 1101 30-31 0.14803759607984546 1101 32-33 0.1756738809531555 1101 34-35 -0.15019820941144957 1101 36-37 -0.12426465537695464 1101 38-39 0.6377837078516677 1101 40-41 0.39747336955854706 1101 42-43 0.4660486058718263 1101 44-45 0.050115634799212216 1101 46-47 0.031329770024647985 1101 48-49 -0.5987740412807625 1101 50-51 -0.08318653840638746 1101 52-53 0.08199931201104249 1101 54-55 0.07000392225312169 1101 56-57 0.1825565739998254 1101 58-59 0.12929240958057164 1101 60-61 0.18764782462626783 1101 62-63 -0.28976462005140036 1101 64-65 -0.8201911239346984 1101 66-67 0.2617624380091428 1101 68-69 0.16554554740073257 1101 70-71 -0.023762070638024824 1101 72-73 -0.20223454202188407 1101 74-75 -0.6025935913218952 1101 76 -0.6643664831028566 1102 1 0.03786992769180131 1102 2 -0.22746556760585435 1102 3 -0.25344445356974177 1102 4 0.14712382421088677 1102 5 -0.006007534928926361 1102 6 0.062199031542817806 1102 7 -0.2520703806624951 1102 8 -0.3225632960614888 1102 9 0.10057481006916902 1102 10-11 -0.20444709829278196 1102 12-13 -0.023123785786864914 1102 14-15 0.1358264716355606 1102 16-17 0.20388986558745614 1102 18-19 0.003699330328515771 1102 20-21 -0.25343536983974246 1102 22-23 0.02227819937952802 1102 24-25 0.027641627967646798 1102 26-27 -0.09907174312726141 1102 28-29 -0.12591260194636078 1102 30-31 -0.09035526106301006 1102 32-33 -0.5145046904754196 1102 34-35 0.2081351239218847 1102 36-37 0.4418067731944788 1102 38-39 0.558617041185002 1102 40-41 -0.20074091615574474 1102 42-43 0.0422390820623022 1102 44-45 0.059044206227781615 1102 46-47 0.3649607224056055 1102 48-49 0.4309878634811355 1102 50-51 0.17425393778407994 1102 52-53 -0.05014354513181729 1102 54-55 0.3289324936816911 1102 56-57 -0.005836283143032972 1102 58-59 -0.165945685657519 1102 60-61 0.22693353891197887 1102 62-63 -0.3171455724323451 1102 64-65 -0.48328636202993636 1102 66-67 -1.0870470858003785 1102 68-69 -1.079394928789739 1102 70-71 -0.9151311182570758 1102 72-73 -1.1352702563075994 1102 74-75 -2.1636054960837967 1102 76 -2.6869855307219055 1103 1 0.21028880674784745 1103 2 0.22364062708441423 1103 3 0.14611306855415052 1103 4 -0.06261068906344747 1103 5 0.13728155061679814 1103 6 0.2290131908348556 1103 7 -0.08038896473329515 1103 8 0.19690573048718107 1103 9 0.11274295166209214 1103 10-11 0.1333110138016096 1103 12-13 0.08838063899190018 1103 14-15 0.34227927399543745 1103 16-17 0.2195609570328756 1103 18-19 0.41701053976804303 1103 20-21 0.26459560361158907 1103 22-23 0.16556728492525252 1103 24-25 -0.178589935454184 1103 26-27 0.010330911739991677 1103 28-29 0.0733868080831428 1103 30-31 -0.05562074778868009 1103 32-33 0.5298537166042365 1103 34-35 -0.2729858200309181 1103 36-37 0.3015412864688116 1103 38-39 0.6184253007720173 1103 40-41 1.2937281103929266 1103 42-43 0.44352964253427274 1103 44-45 0.2271857991481312 1103 46-47 -0.06874134249114405 1103 48-49 0.35539789297966706 1103 50-51 0.09147370179588421 1103 52-53 0.3797237115053491 1103 54-55 0.018091785717089692 1103 56-57 0.3904026549100621 1103 58-59 0.8992829279117984 1103 60-61 0.7398760167880809 1103 62-63 0.48314941281838486 1103 64-65 0.17696437543319377 1103 66-67 -0.04670785276203304 1103 68-69 0.7801552187028875 1103 70-71 -0.21564734244586603 1103 72-73 0.9156146994446104 1103 74-75 1.3815641204353746 1103 76 0.34811771411585113 1104 1 0.06935140917328653 1104 2 -0.013576678716965773 1104 3 0.16692591680062918 1104 4 -0.06769099060392847 1104 5 -0.19211864604003637 1104 6 -0.08039356104977458 1104 7 0.25163332304120445 1104 8 0.12373300023480738 1104 9 0.0496488841432452 1104 10-11 0.1983306794849895 1104 12-13 0.24169102902795458 1104 14-15 0.1251783234874111 1104 16-17 0.2941676433652347 1104 18-19 0.11805118218036625 1104 20-21 0.06462018571581751 1104 22-23 0.15098190308322756 1104 24-25 0.45403051685653395 1104 26-27 0.4708356642801377 1104 28-29 0.44584665731290585 1104 30-31 0.3661262204184652 1104 32-33 0.3600323465616242 1104 34-35 0.4655425313292909 1104 36-37 0.36680677319447597 1104 38-39 1.9410244485924082 1104 40-41 0.5677776023627743 1104 42-43 0.06955389687711744 1104 44-45 0.0544145765981483 1104 46-47 0.37144220388709215 1104 48-49 -0.09678991429663597 1104 50-51 0.06545764148778943 1104 52-53 -0.006625026613299667 1104 54-55 0.09282138257057682 1104 56-57 -0.12250294980970722 1104 58-59 0.6798876476758053 1104 60-61 0.2422113166897546 1104 62-63 0.6884099831232007 1104 64-65 0.11300993426636552 1104 66-67 0.3305455067922125 1104 68-69 0.81551247861767 1104 70-71 2.0570911039651456 1104 72-73 1.5175075214701792 1104 74-75 1.778524133545833 1104 76 2.477829284092909 1105 1 0.09857329160617923 1105 2 0.09982801037580202 1105 3 0.04884912441190892 1105 4 0.09116052145859754 1105 5 0.18810561491816458 1105 6 0.07183205906575552 1105 7 0.16260851842007185 1105 8 0.0361523002687818 1105 9 0.016859213738896983 1105 10-11 -0.19221468239064876 1105 12-13 0.025500067424147232 1105 14-15 -0.32338606659074287 1105 16-17 0.12457029372200878 1105 18-19 0.4194867921022194 1105 20-21 0.04920224483915803 1105 22-23 -0.022140760865120512 1105 24-25 0.05864315701963818 1105 26-27 -0.04207633028322988 1105 28-29 0.21907975279371072 1105 30-31 0.029140151781021473 1105 32-33 0.11083934622183733 1105 34-35 -0.016023897484842564 1105 36-37 -0.27883542864038446 1105 38-39 0.26679746931955606 1105 40-41 0.21554348751397612 1105 42-43 0.22415804230694647 1105 44-45 0.1645487933837444 1105 46-47 0.3150371750049956 1105 48-49 -0.24523537810907214 1105 50-51 -0.4750197625217254 1105 52-53 0.5872417759690975 1105 54-55 -0.250195946685281 1105 56-57 0.33430133153586183 1105 58-59 0.7345512562384968 1105 60-61 0.5252133554257341 1105 62-63 0.3109889841425755 1105 64-65 0.07397663491196482 1105 66-67 0.36792233315986067 1105 68-69 0.5889154687637799 1105 70-71 0.8207251508560773 1105 72-73 0.5229866244263448 1105 74-75 0.309062699634854 1105 76 1.030751472336199 1106 1 -0.3019851447719617 1106 2 -0.2524655676058529 1106 3 -0.22518358400452598 1106 4 -0.19635443665868024 1106 5 0.13928232014353625 1106 6 0.04263381415151457 1106 7 0.11314701064185329 1106 8 0.32526279089503163 1106 9 0.10166176659090809 1106 10-11 0.29845145243184845 1106 12-13 0.12035447508270636 1106 14-15 -0.07268802111806139 1106 16-17 0.18196957573238137 1106 18-19 -0.07764124938162809 1106 20-21 0.223195064942864 1106 22-23 0.13278544575633333 1106 24-25 -0.02145257493090469 1106 26-27 0.28081956122056084 1106 28-29 0.32825406472031204 1106 30-31 0.18681865198045955 1106 32-33 0.29364748343762415 1106 34-35 0.2530626601537662 1106 36-37 0.22224155580317273 1106 38-39 0.08506631654731933 1106 40-41 -0.004001785720966211 1106 42-43 0.23444922698983106 1106 44-45 0.2851311627495221 1106 46-47 0.3754679687824165 1106 48-49 0.3096110518869324 1106 50-51 0.29255103923335923 1106 52-53 0.8205086287812193 1106 54-55 0.8387151023773427 1106 56-57 0.5648158907700136 1106 58-59 0.6556122853569661 1106 60-61 0.7894335389119789 1106 62-63 0.6973471811908425 1106 64-65 0.901858565506295 1106 66-67 1.1959239286923733 1106 68-69 1.1468731871522877 1106 70-71 1.3149413455110412 1106 72-73 0.9234253958663139 1106 74-75 1.6289669676843168 1106 76 2.0767825852201227 1107 1 -0.015028623032833366 1107 2 0.13014312804632056 1107 3 0.2617729377346052 1107 4 0.0906020850804552 1107 5 0.10449971144788606 1107 6 -0.2878009684571765 1107 7 0.24358179325054863 1107 8 0.19482800828633628 1107 9 0.13644437528655828 1107 10-11 -0.01893985191598091 1107 12-13 0.17687621421313793 1107 14-15 0.20122502236019812 1107 16-17 -0.135421728615448 1107 18-19 -0.02546733633815279 1107 20-21 0.40580376059504175 1107 22-23 -0.1367797716349699 1107 24-25 0.022025685938665163 1107 26-27 0.38081956122056226 1107 28-29 0.3369497168942246 1107 30-31 -0.09144221758475624 1107 32-33 0.2632127008289302 1107 34-35 -0.029546035498405843 1107 36-37 0.20485025145534763 1107 38-39 1.237240229590796 1107 40-41 0.8525199534094696 1107 42-43 -0.08728990344494747 1107 44-45 0.19382681492343323 1107 46-47 0.19720709921720214 1107 48-49 -0.03821503506958379 1107 50-51 0.6273336479290137 1107 52-53 0.12920428095513614 1107 54-55 -0.20911098457918342 1107 56-57 -0.22648845705607812 1107 58-59 -0.022648584208248224 1107 60-61 -0.6888273306532398 1107 62-63 -0.028739775330898 1107 64-65 0.26707595681064333 1107 66-67 0.2480978417358557 1107 68-69 -0.3183442041520621 1107 70-71 -0.42853691535852434 1107 72-73 0.784294961083706 1107 74-75 -0.5536417279678538 1107 76 0.6159130200027327 1108 1 -0.22372427520674876 1108 2 0.121447475872408 1108 3 0.2617729377346052 1108 4 0.1253846937761054 1108 5 1.5188536092836102E-4 1108 6 0.20785120545586722 1108 7 0.14792961933750348 1108 8 0.19482800828633628 1108 9 0.2668791578952536 1108 10-11 -0.001548547568148706 1108 12-13 0.08991969247401244 1108 14-15 0.17513806583846048 1108 16-17 0.26892609747151397 1108 18-19 0.4919239680096723 1108 20-21 0.4971081084211306 1108 22-23 0.24582892401721068 1108 24-25 0.46985177289518276 1108 26-27 0.20690651774230417 1108 28-29 0.17173232558987905 1108 30-31 0.39116647806741867 1108 32-33 0.5153866138724084 1108 34-35 0.18349744276246582 1108 36-37 0.5309372079770824 1108 38-39 0.5241967513299244 1108 40-41 0.38295473601817065 1108 42-43 0.03879705307678449 1108 44-45 -0.08443405464178255 1108 46-47 -0.007140726869756975 1108 48-49 0.31830670406085204 1108 50-51 0.601246691407276 1108 52-53 0.7292042809551376 1108 54-55 0.33436727629038643 1108 56-57 0.5474245864221814 1108 58-59 0.2469166331830479 1108 60-61 0.8459552780424104 1108 62-63 0.35821674640822465 1108 64-65 0.8453368263758634 1108 66-67 1.3089674069532435 1108 68-69 1.9077427523696784 1108 70-71 1.8192891715979975 1108 72-73 1.8277732219532687 1108 74-75 1.5333147937712717 1108 76 1.7550434547853406 1109 1 0.07754544391599438 1109 2 0.25903885717291075 1109 3 -0.04857719693257678 1109 4 0.14092913394539863 1109 5 0.19922845327166527 1109 6 -0.09842043748372475 1109 7 -7.429470341762112E-4 1109 8 0.2500030756199223 1109 9 0.21008808440544868 1109 10-11 0.13773579256267254 1109 12-13 0.11050453279720784 1109 14-15 0.4130757341724305 1109 16-17 0.05141936411252601 1109 18-19 0.24886894684768635 1109 20-21 0.21149825847884784 1109 22-23 0.25406286014648316 1109 24-25 0.3037109495015713 1109 26-27 -0.06489032719806431 1109 28-29 0.1928558346318141 1109 30-31 0.19659164159185138 1109 32-33 -0.08961530994444189 1109 34-35 0.218164622446956 1109 36-37 0.21747049000862972 1109 38-39 0.5830270706835279 1109 40-41 0.4397458095079756 1109 42-43 0.34175973102984614 1109 44-45 0.34223004693574666 1109 46-47 0.3427630822876111 1109 48-49 0.3332739991743523 1109 50-51 0.21094272834455552 1109 52-53 0.33990070265578964 1109 54-55 0.5402156795223974 1109 56-57 0.32845575225519497 1109 58-59 0.06299974207108505 1109 60-61 0.4611149548411788 1109 62-63 0.005273306623699625 1109 64-65 -0.0575488989030859 1109 66-67 0.21435409414062434 1109 68-69 0.1429870771099715 1109 70-71 -0.2377712362511737 1109 72-73 -0.8410224686969805 1109 74-75 -0.7998518087681639 1109 76 -0.6961300734947677 1110 1 0.11434967847061728 1110 2 0.21669331089881894 1110 3 0.04421909783212641 1110 4 0.022824758790328303 1110 5 0.10279308812403087 1110 6 0.05425510630917785 1110 7 0.015219338963667894 1110 8 0.14753016188243606 1110 9 0.27931312782617823 1110 10-11 0.03542829111531631 1110 12-13 0.3497734104748176 1110 14-15 0.04007101992215922 1110 16-17 0.2846920462728164 1110 18-19 0.24556070104502226 1110 20-21 0.042943134833159036 1110 22-23 -0.009108093455360233 1110 24-25 0.1932568927651559 1110 26-27 0.3017460138820738 1110 28-29 0.26563724229040275 1110 30-31 0.13464473893698425 1110 32-33 0.1979953095245861 1110 34-35 0.7411569307754675 1110 36-37 0.42171331525054967 1110 38-39 0.23533043682363086 1110 40-41 0.49668899038631054 1110 42-43 0.21579827209345126 1110 44-45 0.11979186977917777 1110 46-47 0.3868454576080964 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-0.11559872989747788 2116 6 0.012199031542820649 2116 7 0.2649107514129767 2116 8 0.0680027416743556 2116 9 -0.07159500125158758 2116 10-11 -0.06922697250661969 2116 12-13 -0.10142567257931034 2116 14-15 0.2458107483651233 2116 16-17 -0.8240189394439952 2116 18-19 -0.20942960048910209 2116 20-21 0.13406463016026038 2116 22-23 0.12495115535437407 2116 24-25 0.07850640784185714 2116 26-27 0.039843351212354605 2116 28-29 -0.13495348244949668 2116 30-31 0.12992775780490717 2116 32-33 0.25931606424156683 2116 34-35 0.2814055641734541 2116 36-37 -0.06951398152250476 2116 38-39 -1.2666974242238034 2116 40-41 -1.7172503501180074 2116 42-43 -0.5785942512710349 2116 44-45 -0.1437859824514618 2116 46-47 -0.11161160463841924 2116 48-49 -0.12168509249370629 2116 50-51 -0.30412656536056915 2116 52-53 -0.24684165833937044 2116 54-55 -0.3196524119786801 2116 56-57 0.10312598100790638 2116 58-59 -0.6919676982361409 2116 60-61 -0.3869815554276457 2116 62-63 -0.26651664161473576 2116 64-65 -0.6699215821557161 2116 66-67 -0.2982106078129547 2116 68-69 -0.869410652060175 2116 70-71 -0.1454770302067594 2116 72-73 -0.9984778034774138 2116 74-75 -0.9965457476561248 2116 76 -0.6970484238036647 2117 1 -0.06838007230819443 2117 2 -0.027465567605851504 2117 3 -0.10969445356974461 2117 4 0.1429571575442239 2117 5 -0.0914242015955935 2117 6 0.14761569820948495 2117 7 0.21459628600416636 2117 8 -1.6725632960614902 2117 9 -0.5973418565974953 2117 10-11 -0.03778043162611766 2117 12-13 -0.04708211912019067 2117 14-15 0.12749313830222775 2117 16-17 7.648655874561427E-4 2117 18-19 0.1828659969951829 2117 20-21 0.20177296349358897 2117 22-23 -0.03188846728713912 2117 24-25 0.08076662796764111 2117 26-27 0.2353032568727329 2117 28-29 -1.323829268613025 2117 30-31 -0.026813594396344342 2117 32-33 -0.1092963571420853 2117 34-35 -0.19186487607811387 2117 36-37 -0.49256822680552403 2117 38-39 -0.023674625481660883 2117 40-41 -0.44553258282241615 2117 42-43 -0.7348442512710349 2117 44-45 -0.06595579377221839 2117 46-47 0.2232940557389398 2117 48-49 -0.0325538031855217 2117 50-51 -0.31012106221591296 2117 52-53 -0.5772268784651473 2117 54-55 0.011224160348355383 2117 56-57 0.03895538352363559 2117 58-59 0.48717931434246964 2117 60-61 -0.33244146108802397 2117 62-63 0.09118776090097924 2117 64-65 -0.4801613620299392 2117 66-67 -0.3870470858003827 2117 68-69 -0.08668659545640622 2117 70-71 0.6629938817429242 2117 72-73 -0.5727702563075994 2117 74-75 0.6228528372495354 2117 76 0.6421811359447602 2118 1 0.03231437213624844 2118 2 -0.1676170827573671 2118 3 -0.3600101101353985 2118 4 0.014800591887656367 2118 5 -0.04060349452488765 2118 6 0.04755256689635701 2118 7 -0.138939067531183 2118 8 -0.2912501647483623 2118 9 0.031130365624726153 2118 10-11 0.12888623504054664 2118 12-13 0.17182570916263273 2118 14-15 -0.010764437455350162 2118 16-17 0.11108683528442498 2118 18-19 -0.46991178078259566 2118 20-21 -0.030076783981151323 2118 22-23 0.08869234079367061 2118 24-25 0.14973758756360667 2118 26-27 -0.05273335928888656 2118 28-29 0.11714295360919635 2118 30-31 0.1851497894420362 2118 32-33 -0.11008549855621652 2118 34-35 0.3434886592754154 2118 36-37 0.34029162167932725 2118 38-39 -1.069918312350353 2118 40-41 0.3207237303088988 2118 42-43 -0.21495788763467516 2118 44-45 -0.11267296548938788 2118 46-47 0.3192536516985385 2118 48-49 0.04588685338013221 2118 50-51 -0.8364783854482383 2118 52-53 -1.2751435451318187 2118 54-55 -0.4071786174294232 2118 56-57 -0.12881608112283516 2118 58-59 -0.7557184129302499 2118 60-61 -0.41056646108802397 2118 62-63 -0.3292667845535604 2118 64-65 -0.9800035337471087 2118 66-67 -0.7064915302448256 2118 68-69 -1.0704302823250913 2118 70-71 -1.4896260677520239 2118 72-73 0.20563883460149057 2118 74-75 -0.4584287284070321 2118 76 0.31200436826799205 2119 1 0.08599726851577572 2119 2 0.17899510655145434 2119 3 0.015656670025762764 2119 4 0.12352831859291058 2119 5 0.0051347871684512825 2119 6 0.09646869446417128 2119 7 -0.21611532448272186 2119 8 0.29653782753401714 2119 9 0.21714784377703467 2119 10-11 0.2805716282989721 2119 12-13 0.24429194455021275 2119 14-15 -0.37508176806480975 2119 16-17 0.22940484686086648 2119 18-19 0.4014989557966828 2119 20-21 -0.020429751862209855 2119 22-23 0.07836434170162221 2119 24-25 0.36804424969049165 2119 26-27 0.24882151529970287 2119 28-29 0.29566979505738544 2119 30-31 0.31442001983586465 2119 32-33 0.38338856795154896 2119 34-35 0.1770489815997891 2119 36-37 0.5648404810596404 2119 38-39 0.4671376404359364 2119 40-41 0.3596523422712252 2119 42-43 0.3483720408638007 2119 44-45 0.3792689253289083 2119 46-47 0.6614963029299545 2119 48-49 0.4698455413837621 2119 50-51 0.35566779546199 2119 52-53 0.20238454475582301 2119 54-55 -0.17808997822842088 2119 56-57 0.41073675056482983 2119 58-59 0.4696816551664469 2119 60-61 0.6062874714962447 2119 62-63 1.3360379481668971 2119 64-65 0.9988297428389856 2119 66-67 1.848439805585393 2119 68-69 0.9703709888132579 2119 70-71 2.1696067094582823 2119 72-73 0.9461904178497029 2119 74-75 1.191497500170886 2119 76 0.1171343194653609 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 4.0 23 5.0 24 22.0 25 39.0 26 107.0 27 178.0 28 289.0 29 490.0 30 860.0 31 1195.0 32 1894.0 33 3467.0 34 5788.0 35 10332.0 36 9131.0 37 7290.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.81534118563224 5.139686556994063 15.750024335637109 65.2949479217366 2 7.750900418572957 11.479120023362212 57.777669619390636 22.992309938674193 3 7.845809403290178 5.95979752749927 59.05529056750706 27.139102501703494 4 6.495181543852818 49.62523118855252 28.501898179694347 15.37768908790032 5 7.451572082157111 21.035724715273048 63.31402706122846 8.19867614134138 6 12.744573152925142 56.38080404945002 14.956682565949578 15.917940231675265 7 11.454784386255232 19.186216295142607 22.48369512313832 46.87530419546383 8 9.554171128200137 57.60732015964179 5.906259125863915 26.932249586294173 9 7.870145040397157 15.976345760732016 4.263603621142802 71.88990557772802 10-11 14.435899931860217 38.85184464129271 24.65930108050229 22.052954346344787 12-13 14.092767448651806 9.857149810182031 35.62007203348584 40.430010707680324 14-15 10.47284142898861 4.870777766961939 15.78166066387618 68.87472014017327 16-17 35.37671566241604 27.377591745351893 4.356079042149323 32.88961355008274 18-19 44.63764236347708 19.97712450111944 3.9594081573055586 31.42582497809793 20-21 20.07203348583666 8.749878321814466 4.0494500146013825 67.12863817774749 22-23 11.095833738927286 33.71337486615399 4.0494500146013825 51.14134138031734 24-25 35.43147084590675 30.79309841331646 7.457655991433857 26.317774749342938 26-27 40.54073785651708 40.102696388591454 5.000973425484279 14.355592329407186 28-29 16.040835199065512 36.55821084395989 8.36415847366884 39.03679548330575 30-31 13.316460624939161 20.136522924170155 29.834274311301467 36.71274213958922 32-33 9.843765209773192 29.699211525357732 14.915311982867713 45.541711282001366 34-35 10.020198578798793 11.400029202764529 33.7060741750219 44.87369804341478 36-37 37.520685291540936 23.43643531587657 16.009198870826438 23.033680521756057 38-39 15.74759077192641 19.72646743891755 33.42134722087024 31.104594568285798 40-41 12.720237515818164 5.04721113598754 37.98549596028424 44.24705538791006 42-43 37.08629416918135 4.468022972841429 33.33130536357442 25.114377494402802 44-45 14.132921249878322 29.056750705733474 42.1286381777475 14.68168986664071 46-47 32.80322203835296 11.06663097439891 26.676725396670886 29.45342159057724 48-49 32.63652292417015 5.007057334761024 11.904993672734353 50.45142606833447 50-51 21.759709919205687 5.415896038158279 33.06969726467439 39.75469677796165 52-53 7.2069989292319665 5.288133943346637 65.4166261072715 22.08824102014991 54-55 10.515428793925825 8.687822447191667 44.968607028132 35.82814173075051 56-57 31.837097245205882 12.517034945974887 19.9041175897985 35.74175021902074 58-59 13.385817190694052 14.64396962912489 50.271342353742824 21.698870826438235 60-61 31.52681787209189 34.32176579382848 23.262435510561666 10.88898082351796 62-63 12.369804341477659 71.1440183003991 5.6677698822155165 10.81840747590772 64-65 6.622943638664461 77.21941010415652 5.184707485641974 10.972938771537038 66-67 6.928355884357053 75.96004088387033 5.6933223011778455 11.418280930594763 68-69 6.912537720237516 75.4769784872968 5.714007592718778 11.89647619974691 70-71 7.084103961841722 70.07203348583666 7.361530224861287 15.482332327460332 72-73 7.883529640805996 57.382215516402226 11.602014990752457 23.132239852039326 74-75 8.681738537914923 50.372335247736785 14.289886109218338 26.656040105129954 76 8.308186508322787 51.02209675849314 14.221746325318795 26.447970407865277 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.5 24 1.5 25 2.0 26 1.5 27 2.0 28 1.5 29 0.0 30 1.5 31 2.5 32 6.0 33 10.0 34 8.0 35 8.0 36 10.0 37 10.0 38 23.0 39 209.0 40 3412.5 41 7862.0 42 9281.0 43 8160.5 44 5474.5 45 2847.0 46 1785.0 47 1293.5 48 571.5 49 257.0 50 173.0 51 152.5 52 111.5 53 107.5 54 124.0 55 167.5 56 251.0 57 390.5 58 490.0 59 552.0 60 703.0 61 816.0 62 840.0 63 851.5 64 885.5 65 905.0 66 853.0 67 804.0 68 761.5 69 715.5 70 671.5 71 631.0 72 579.0 73 484.5 74 389.0 75 336.0 76 279.5 77 168.5 78 85.5 79 57.0 80 40.0 81 14.0 82 2.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 41092.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.33505305168889 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46880346754875 15.034556604691913 2 19.77871563818866 8.439598948700478 3 6.547279571118969 4.190596709821863 4 1.4600205315387247 1.2459846198773483 5 0.5475076993270217 0.584055290567507 6 0.20531538724763318 0.2628248807553782 7 0.07984487281852401 0.11924462182419934 8 0.045625641610585146 0.07787403874233427 9 0.05703205201323143 0.10951036698140758 >10 0.5018820577164366 2.353256108244914 >50 0.11406410402646286 1.7813686362308967 >100 0.12547051442910914 5.207826340893605 >500 0.022812820805292573 3.443492650637594 >1k 0.034219231207938856 22.792757714396963 >5k 0.0 0.0 >10k+ 0.011406410402646287 34.3570524676336 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 14118 34.3570524676336 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 3556 8.653752555241896 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 3131 7.619487978195269 No Hit CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG 2679 6.519517180959798 RNA PCR Primer, Index 32 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 866 2.107466173464421 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 549 1.3360264771731725 RNA PCR Primer, Index 42 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT 478 1.1632434537136183 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT 268 0.6521950744670496 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 200 0.4867127421395892 No Hit CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 189 0.45994354132191184 No Hit CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 181 0.4404750316363283 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT 169 0.4112722671079529 No Hit CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 152 0.3699016840260878 RNA PCR Primer, Index 32 (95% over 21bp) TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT 145 0.35286673805120217 RNA PCR Primer, Index 42 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG 134 0.3260975372335248 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC 116 0.28229339044096174 No Hit CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 108 0.2628248807553782 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT 98 0.23848924364839869 No Hit CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 98 0.23848924364839869 No Hit CAGCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGC 90 0.21902073396281516 No Hit ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT 80 0.19468509685583568 RNA PCR Primer, Index 42 (95% over 24bp) CTTATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 77 0.18738440572374185 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC 70 0.1703494597488562 No Hit CTCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 60 0.14601382264187676 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG 56 0.13627956779908498 No Hit CACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTTCTG 52 0.1265453129562932 RNA PCR Primer, Index 42 (95% over 23bp) TCCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 51 0.12411174924559525 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG 46 0.11194393069210552 No Hit CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 43 0.10464323956001167 No Hit CACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTTCTG 42 0.10220967584931373 RNA PCR Primer, Index 42 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC 42 0.10220967584931373 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004867127421395892 0.0 2 0.0 0.0 0.0 0.014601382264187676 0.0 3 0.0 0.0 0.0 0.014601382264187676 0.0 4 0.0 0.0 0.0 0.046237710503260976 0.0 5 0.0 0.0 0.0 0.058405529056750706 0.0 6 0.0 0.0 0.0 0.058405529056750706 0.0 7 0.0 0.0 0.0 0.0632726564781466 0.0 8 0.0 0.0 0.0 0.06570622018884455 0.0 9 0.0 0.0 0.0 0.09247542100652195 0.0 10 0.0 0.0 0.0 0.10707680327070963 0.0 11 0.0 0.0 0.0 0.11924462182419936 0.0 12 0.0 0.0 0.0 0.11924462182419936 0.0 13 0.0 0.0 0.0 0.1314124403776891 0.0 14 0.0 0.0 0.0 0.1314124403776891 0.0 15 0.0 0.0 0.0 0.13627956779908498 0.0 16 0.0 0.0 0.0 0.13627956779908498 0.0 17 0.0 0.0 0.0 0.15574807748466854 0.0 18 0.0 0.0 0.0 0.16304876861676237 0.0 19 0.0 0.0 0.0 0.21415360654141927 0.0 20 0.0 0.0 0.0 0.24092280735909666 0.0 21 0.0 0.0 0.0 0.25065706220188844 0.0 22 0.0 0.0 0.0 0.2652584444660761 0.0 23 0.0 0.0 0.0 0.27255913559816997 0.0 24 0.0 0.0 0.0 0.27985982673026377 0.0 25 0.0 0.0 0.0 0.30906259125863916 0.0 26 0.0 0.0 0.0 0.31879684610143094 0.0 27 0.0 0.0 0.0 0.39423732113306725 0.0 28 0.0 0.0 0.0 0.4818456147181933 0.0 29 0.0 0.0 0.0 0.5597196534605275 0.0 30 0.0 0.0 0.0 0.6400272559135598 0.0 31 0.0 0.0 0.0 0.7811739511340406 0.0 32 0.0 0.0 0.0 0.9636912294363866 0.0 33 0.0 0.0 0.0 1.216781855348973 0.0 34 0.0 0.0 0.0 1.5404458288718 0.0 35 0.0 0.0 0.0 1.9711866056653362 0.0 36 0.0 0.0 0.0 2.574710405918427 0.0 37 0.0 0.0 0.0 3.477562542587365 0.0 38 0.0 0.0 0.0 4.163827509004186 0.0 39 0.0 0.0 0.0 4.730847853596807 0.0 40 0.0 0.0 0.0 5.370875109510367 0.0 41 0.0 0.0 0.0 6.027937311398812 0.0 42 0.0 0.0 0.0 6.568188455173757 0.0 43 0.0 0.002433563710697946 0.0 7.0062299230993865 0.0 44 0.0 0.002433563710697946 0.0 7.578117395113404 0.0 45 0.0 0.002433563710697946 0.0 8.09159933807067 0.0 46 0.0 0.002433563710697946 0.0 8.665920373795386 0.0 47 0.0 0.002433563710697946 0.0 9.18183588046335 0.0 48 0.0 0.002433563710697946 0.0 9.692884259709919 0.0 49 0.0 0.002433563710697946 0.0 10.121191472792757 0.0 50 0.0 0.002433563710697946 0.0 10.544631558454201 0.0 51 0.0 0.002433563710697946 0.0 10.909666115058892 0.0 52 0.0 0.002433563710697946 0.0 11.204127324053344 0.0 53 0.0 0.002433563710697946 0.0 11.559427625815244 0.0 54 0.0 0.002433563710697946 0.0 11.86118952594179 0.0 55 0.0 0.002433563710697946 0.0 12.162951426068334 0.0 56 0.0 0.002433563710697946 0.0 12.442811252798599 0.0 57 0.0 0.002433563710697946 0.0 12.749440280346539 0.0 58 0.0 0.002433563710697946 0.0 13.10474058210844 0.0 59 0.0 0.002433563710697946 0.0 13.455173756448945 0.0 60 0.0 0.002433563710697946 0.0 13.727732892047113 0.0 61 0.0 0.002433563710697946 0.0 13.968655699406211 0.0 62 0.0 0.002433563710697946 0.0 14.192543560790421 0.0 63 0.0 0.002433563710697946 0.0 14.423732113306727 0.0 64 0.0 0.002433563710697946 0.0 14.713326194879782 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATATA 15 0.0022096108 70.00001 2 CTTATAT 15 0.0022096108 70.00001 1 TCTTTAT 120 0.0 70.00001 1 GCTCACC 15 0.0022096108 70.00001 15 ATATACA 15 0.0022096108 70.00001 4 AAAAAGG 20 7.838043E-5 69.99999 68 CTTTATA 130 0.0 64.61539 2 TTTATAC 135 0.0 62.22222 3 ACTCGAG 25 2.3638854E-4 55.999996 32 TCGAAAA 25 2.3638854E-4 55.999996 58 TCTGCTC 25 2.3638854E-4 55.999996 53 TATATAC 20 0.006904618 52.499996 3 TCTATAC 20 0.006904618 52.499996 1 CGAGGAA 20 0.006904618 52.499996 24 TCTGCCT 20 0.006904618 52.499996 53 CCTTATA 20 0.006904618 52.499996 1 CTGCCTG 20 0.006904618 52.499996 54 ACGCGCG 20 0.006904618 52.499996 38 AAACTAA 50 6.0073944E-8 48.999996 70 CTCGAAA 30 5.813037E-4 46.666668 57 >>END_MODULE