FastQCFastQC Report
Wed 25 May 2016
SRR1780321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72397
Sequences flagged as poor quality0
Sequence length50
%GC63

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACATC250.002361995935.1275756
ACATCTC300.005767605729.272988
CTCCAAG407.023909E-427.4623971
AGACCCA551.56592E-424.01701227
ACGAGAC551.56592E-424.01701224
CTCGGTG1301.8189894E-1223.65991244
GAGACCC703.144426E-522.01559326
CGAAAAT500.002546712522.0155925
GAAAATT500.002546712522.0155926
AAAATTT500.002546712522.0155927
ACGAAAA500.002546712522.0155924
AGATCTC1551.8189894E-1221.32018740
GACCCAA654.8474176E-420.32208628
ACCCAAT654.8474176E-420.32208629
TCTCGGT1201.3871613E-820.15302843
GACGAAA1001.0936237E-619.8003123
TAGATCT1252.1649612E-819.38715639
CCCAATG707.984546E-418.87050630
GTAGATC1303.3951437E-818.64149738
CACATCT4850.018.5596737
Produced by FastQC (version 0.11.5)