SRR1780321_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780321_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72397
Sequences flagged as poor quality	0
Sequence length	50
%GC	60

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT	20734	28.639308258629505	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG	5227	7.219912427310524	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC	4560	6.298603533295578	No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG	3217	4.443554290923657	Illumina PCR Primer Index 4 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC	1229	1.697584154039532	No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT	767	1.0594361644819537	RNA PCR Primer, Index 27 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC	287	0.3964252662403138	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG	266	0.36741853944224206	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCATCTCGTATGCCGTCT	248	0.3425556307581806	RNA PCR Primer, Index 20 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG	224	0.3094050858460986	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC	191	0.26382308659198583	No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT	173	0.23896017790792437	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCATCTCGTATGCCGTC	170	0.23481635979391413	RNA PCR Primer, Index 4 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG	134	0.18509054242579112	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGC	128	0.17680290619777064	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC	116	0.16022763374172963	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT	109	0.15055872480903906	No Hit
CTTATATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC	97	0.13398345235299805	No Hit
TCCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC	87	0.12017072530629723	No Hit
CTCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG	77	0.10635799825959638	No Hit
ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCT	76	0.1049767255549263	RNA PCR Primer, Index 27 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTAAT	40	8.039933E-9	44.05467	1
TCTTTAT	150	0.0	42.586178	1
TCTTCTG	375	0.0	40.502266	44
CGTCTTC	380	0.0	39.969345	42
GTCTTCT	385	0.0	39.45026	43
TTTATAC	195	0.0	33.84137	3
ATGCCGT	2790	0.0	33.688774	44
CTTTATA	190	0.0	33.5742	2
CCCATCT	40	1.7983892E-5	32.995335	33
CCGTCTT	475	0.0	31.975477	41
CCTTATA	30	0.0056752455	29.369781	1
GACCCAT	45	4.0214047E-5	29.329185	31
TATGCCG	3350	0.0	28.057215	43
TGACCCA	55	4.882355E-6	27.996042	30
GCCGTCT	550	0.0	27.615183	40
CTTATAC	3530	0.0	27.518568	1
TGACCAT	65	5.809834E-7	27.073095	30
TATACAC	3675	0.0	26.396269	3
TTATACA	3675	0.0	26.396269	2
TTAATAC	75	6.832852E-8	26.396269	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content