FastQCFastQC Report
Wed 25 May 2016
SRR1780320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152372
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGTC150.00222119270.06
CAGCGAA200.00694052652.538
GAAGCAA200.00694052652.547
ACGTGTC200.00694052652.531
ACGGAAG200.00694052652.544
AAGGGTT200.00694052652.543
GACGAAT200.00694052652.523
CGGGCCA305.863271E-446.6666645
CTGAGAC350.001252343940.047
TGTATAG400.002412874535.049
TAGATCT652.0921374E-532.30769339
AACCGAT500.007191060627.99999828
CCGATAC500.007191060627.99999839
ACCGATA500.007191060627.99999829
TACGGTT500.007191060627.99999843
CTATAAA700.00122167625.01
GGTTGTA1001.3884348E-524.49999846
CGACGAA750.001825776323.33333422
GAATCGT1953.092282E-1123.33333470
ATATCGC750.001825776323.33333433
Produced by FastQC (version 0.11.5)