##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780320_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152372 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.539108235108813 34.0 32.0 34.0 12.0 34.0 2 30.682901057937155 34.0 32.0 34.0 21.0 34.0 3 30.486152311448297 34.0 32.0 34.0 11.0 34.0 4 30.3719843540808 34.0 32.0 34.0 11.0 34.0 5 30.39256556322684 34.0 32.0 34.0 11.0 34.0 6 33.34577218911611 38.0 34.0 38.0 21.0 38.0 7 33.73170923791773 38.0 34.0 38.0 21.0 38.0 8 33.64891843645814 38.0 34.0 38.0 21.0 38.0 9 33.9143477804321 38.0 35.0 38.0 21.0 38.0 10-11 33.687117711915576 38.0 34.0 38.0 21.0 38.0 12-13 33.74527144094715 38.0 34.0 38.0 21.0 38.0 14-15 33.68236618276323 38.0 34.0 38.0 21.0 38.0 16-17 33.743417425773764 38.0 34.0 38.0 21.0 38.0 18-19 33.7366478093088 38.0 34.0 38.0 21.0 38.0 20-21 33.743863701992495 38.0 34.0 38.0 21.0 38.0 22-23 33.75927992019531 38.0 34.0 38.0 21.0 38.0 24-25 33.78053054366944 38.0 34.0 38.0 21.0 38.0 26-27 33.74912713621926 38.0 34.0 38.0 21.0 38.0 28-29 33.84384270075867 38.0 35.0 38.0 21.0 38.0 30-31 33.85858294174783 38.0 35.0 38.0 21.0 38.0 32-33 33.90541897461476 38.0 35.0 38.0 21.0 38.0 34-35 33.896401569842226 38.0 35.0 38.0 21.0 38.0 36-37 33.81544837634211 38.0 35.0 38.0 21.0 38.0 38-39 33.82635261071588 38.0 35.0 38.0 21.0 38.0 40-41 33.87450778358229 38.0 35.0 38.0 21.0 38.0 42-43 33.93081406032604 38.0 35.0 38.0 21.0 38.0 44-45 34.020400729792875 38.0 36.0 38.0 21.0 38.0 46-47 33.9432933872365 38.0 35.5 38.0 21.0 38.0 48-49 33.89501351954427 38.0 35.0 38.0 21.0 38.0 50-51 33.84847937941355 38.0 35.0 38.0 21.0 38.0 52-53 33.91084648098076 38.0 35.0 38.0 21.0 38.0 54-55 33.88030609298296 38.0 35.0 38.0 21.0 38.0 56-57 33.84407568319639 38.0 35.0 38.0 21.0 38.0 58-59 33.920812222718084 38.0 35.5 38.0 21.0 38.0 60-61 33.931375187042235 38.0 35.5 38.0 21.0 38.0 62-63 33.80524965216706 38.0 35.0 38.0 20.5 38.0 64-65 33.76593468616281 38.0 35.0 38.0 19.5 38.0 66-67 33.66787533142572 38.0 34.5 38.0 19.0 38.0 68-69 33.61166093507994 38.0 34.0 38.0 19.0 38.0 70-71 33.55811107027537 38.0 34.0 38.0 19.0 38.0 72-73 33.54009266794424 38.0 34.0 38.0 19.0 38.0 74-75 33.5444504239624 38.0 34.0 38.0 19.0 38.0 76 32.19474706639015 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23208432671817647 1101 2 0.2902511205257454 1101 3 0.36927614209822934 1101 4 0.46143991373734394 1101 5 0.24713044959508323 1101 6 0.3475320399179367 1101 7 -0.0404779158951456 1101 8 -0.24068085848755771 1101 9 0.2237640019929401 1101 10-11 -0.31955340937651755 1101 12-13 0.18046477166237906 1101 14-15 0.34390410563734974 1101 16-17 0.03455803237467592 1101 18-19 0.11930598559867889 1101 20-21 0.1643547398627021 1101 22-23 0.3314886783319153 1101 24-25 0.23369681256112074 1101 26-27 -0.19364046550076353 1101 28-29 0.2117022569403062 1101 30-31 -0.11730433007902263 1101 32-33 -0.16320500909417746 1101 34-35 -0.27303105592903876 1101 36-37 0.25599594242094525 1101 38-39 0.10467322216140218 1101 40-41 0.15911722401488504 1101 42-43 -0.17645030494377778 1101 44-45 0.027935205304252975 1101 46-47 0.2802658983148163 1101 48-49 -0.09600348897247102 1101 50-51 -0.1153266457124218 1101 52-53 0.1684756045051259 1101 54-55 0.1724339198746918 1101 56-57 0.23241187728061163 1101 58-59 0.25653618934927636 1101 60-61 0.050262302762099864 1101 62-63 -0.052353614769863555 1101 64-65 0.23643966042381948 1101 66-67 -0.37018918266387146 1101 68-69 -0.06838047005284409 1101 70-71 -0.26215490475523495 1101 72-73 -0.01743821753143493 1101 74-75 0.21823420567706364 1101 76 -0.7668309162553726 1102 1 -0.34411587410086497 1102 2 -0.45693850608140707 1102 3 -0.14934557685073813 1102 4 -0.5635145588983121 1102 5 -0.7826437620626585 1102 6 -0.21009259552083392 1102 7 -0.5388964390285267 1102 8 -0.5568598460685124 1102 9 -0.6504983177803112 1102 10-11 -0.09449076646167498 1102 12-13 -0.3074893038925026 1102 14-15 -0.21740721938010665 1102 16-17 -0.46309713720620493 1102 18-19 -0.5021528070019272 1102 20-21 -0.4836263097997744 1102 22-23 -0.33423092787349873 1102 24-25 -0.38593403924559766 1102 26-27 -0.8167731589407126 1102 28-29 -0.15803128816943968 1102 30-31 -0.25440673584080287 1102 32-33 -0.2619822741661366 1102 34-35 -0.6170536210708235 1102 36-37 -0.7586891999119771 1102 38-39 -0.7009078165471365 1102 40-41 -0.2805093831353105 1102 42-43 -0.34619891173796447 1102 44-45 -0.4332604185918001 1102 46-47 -0.7877239145095203 1102 48-49 -0.46378243821460075 1102 50-51 -0.4737468119386463 1102 52-53 -0.016317571182582924 1102 54-55 -0.5426880113070993 1102 56-57 -0.24776831523509912 1102 58-59 -0.4975270423405078 1102 60-61 -0.47881017132495884 1102 62-63 -0.40454714372253164 1102 64-65 -0.23548056741838508 1102 66-67 -0.13465182284023314 1102 68-69 -0.3239361777466243 1102 70-71 -0.30133924608038853 1102 72-73 -0.17435016144719384 1102 74-75 -0.5132501917871011 1102 76 -0.3112628853652275 1103 1 0.41359056856019905 1103 2 0.35979441224155195 1103 3 0.37534254035219305 1103 4 0.6473157118584538 1103 5 0.5794402900386437 1103 6 -0.03930082572659188 1103 7 0.45174082690312645 1103 8 0.4098268210981644 1103 9 0.3970528709994028 1103 10-11 0.5555567347258545 1103 12-13 0.9956870255157284 1103 14-15 0.3507194837874721 1103 16-17 0.6225987551911061 1103 18-19 0.4958971442468538 1103 20-21 0.630020281365006 1103 22-23 0.5532850100249505 1103 24-25 0.9575807986253437 1103 26-27 0.5686177258314089 1103 28-29 0.5850060377637121 1103 30-31 0.6000896265682556 1103 32-33 0.3471892051208485 1103 34-35 -0.15577333906377788 1103 36-37 0.38422062283358827 1103 38-39 0.09799340598343065 1103 40-41 0.540717397534209 1103 42-43 0.3582874504210878 1103 44-45 0.09213396626782355 1103 46-47 0.21550116611749814 1103 48-49 0.2794048914686442 1103 50-51 0.0719793312149335 1103 52-53 0.336372496069103 1103 54-55 0.7509005784076379 1103 56-57 0.493893976322191 1103 58-59 0.020610911108320806 1103 60-61 0.16729973142261656 1103 62-63 0.27467608685823564 1103 64-65 0.30550170713848246 1103 66-67 0.2553954283410462 1103 68-69 0.5006781876125075 1103 70-71 0.656972211706055 1103 72-73 0.7142033023894001 1103 74-75 0.6635417280108982 1103 76 0.2096626377728512 1104 1 -0.3066873521809228 1104 2 0.14811880015420442 1104 3 -0.3763178767739994 1104 4 0.23330203639341462 1104 5 -0.31191573770441394 1104 6 0.9828053919534838 1104 7 0.34157735853069937 1104 8 0.010174834724615778 1104 9 0.005243445221886134 1104 10-11 0.09181019648111999 1104 12-13 0.07860372281739814 1104 14-15 0.019720537631556567 1104 16-17 0.06251738862492573 1104 18-19 0.03339438709664222 1104 20-21 -0.4371438977394746 1104 22-23 -0.08530774050809953 1104 24-25 0.19580470373036007 1104 26-27 -6.296334313944385E-4 1104 28-29 0.24566824397403764 1104 30-31 0.14341364686087843 1104 32-33 -0.06180032996235951 1104 34-35 -0.12722764389430097 1104 36-37 0.11175291212604321 1104 38-39 0.2974088901390104 1104 40-41 -0.010148960331633816 1104 42-43 0.22746373647849083 1104 44-45 0.029589545514454585 1104 46-47 -0.07444074991808947 1104 48-49 0.014105771305906956 1104 50-51 0.1463611168679435 1104 52-53 -0.16025152667431541 1104 54-55 -0.04380814399021915 1104 56-57 0.23448903060741344 1104 58-59 0.09257865857787095 1104 60-61 -0.23873046243568297 1104 62-63 0.0402693030433241 1104 64-65 0.011932606827222969 1104 66-67 0.09465357112466677 1104 68-69 -0.00868999609947707 1104 70-71 -0.17084069420772607 1104 72-73 -0.05145118380033864 1104 74-75 -0.25920905011815876 1104 76 0.12672755946978498 1105 1 -0.6731898152351086 1105 2 -0.06943605877996362 1105 3 -0.1272807070471771 1105 4 0.349602976832287 1105 5 0.0068034741182430025 1105 6 -0.8331820688615679 1105 7 -0.2363447193913757 1105 8 -0.2964082373757009 1105 9 -0.1962791700938311 1105 10-11 -0.047509104907149435 1105 12-13 -0.5418396269362944 1105 14-15 -0.1752190055838483 1105 16-17 -0.2061794317960306 1105 18-19 -0.3396911480578595 1105 20-21 -0.14948156007713465 1105 22-23 -0.4976454028457624 1105 24-25 -0.2350954306180455 1105 26-27 -0.35544378479726646 1105 28-29 -0.44998782409582105 1105 30-31 0.08741275120480907 1105 32-33 -0.591871982447806 1105 34-35 -0.11585281182982499 1105 36-37 -0.2115117542420677 1105 38-39 -0.3252959911865645 1105 40-41 -0.2826075362384799 1105 42-43 -0.1735662679997958 1105 44-45 -0.36067019474529616 1105 46-47 -0.16749941539054447 1105 48-49 0.04310263650967272 1105 50-51 -0.37307461980916656 1105 52-53 -0.3823294487522446 1105 54-55 -0.2393074722481714 1105 56-57 -0.2694742474937968 1105 58-59 0.051960655890908924 1105 60-61 -0.20418052960988575 1105 62-63 -0.052028054771277255 1105 64-65 -0.7028009354925189 1105 66-67 -0.11477320899176391 1105 68-69 -0.1258418098030134 1105 70-71 -0.17415462165958218 1105 72-73 -0.08803873418099784 1105 74-75 -0.2644934209197771 1105 76 -0.06279326453379497 1106 1 -0.42980357184886486 1106 2 -0.4968851528957252 1106 3 -0.22806634116614433 1106 4 0.23233505956437028 1106 5 -0.373443439462001 1106 6 -0.6387296077424409 1106 7 -1.0903450400583665 1106 8 -0.9733683143357723 1106 9 -0.6740729812209736 1106 10-11 -0.9890996131643135 1106 12-13 -0.48240400083400203 1106 14-15 -0.789809353507529 1106 16-17 -0.4071414327580385 1106 18-19 -0.7759104842771904 1106 20-21 -0.47973649033206556 1106 22-23 -0.8478297863634765 1106 24-25 -0.8599150554376678 1106 26-27 -0.7680139307007536 1106 28-29 -0.5133355874435921 1106 30-31 -1.1590037952117314 1106 32-33 -0.6066066305157776 1106 34-35 -0.5883235509672247 1106 36-37 -0.4873013966983777 1106 38-39 -0.6530016188921905 1106 40-41 -0.5092549628859047 1106 42-43 -0.8610602554937756 1106 44-45 -0.3419272093356369 1106 46-47 -0.29693664482777393 1106 48-49 -0.43178913838210065 1106 50-51 -0.6881459682138455 1106 52-53 -0.2583114914009528 1106 54-55 -0.328084127691497 1106 56-57 -0.7616339396534855 1106 58-59 -0.7518232145596286 1106 60-61 -0.7111229698856647 1106 62-63 -0.5910692604791521 1106 64-65 -0.6676879003794838 1106 66-67 -0.42811295566485086 1106 68-69 -0.39864924927712053 1106 70-71 -1.0564698373081356 1106 72-73 -0.4503443298199272 1106 74-75 -0.30924249900218825 1106 76 -0.6971366988772303 1107 1 0.680063547178559 1107 2 0.11008874211888298 1107 3 -0.003431528236163217 1107 4 0.6172940643707037 1107 5 0.0037500390037408238 1107 6 -0.2853416141508802 1107 7 0.07422003392147047 1107 8 0.1619440973125137 1107 9 0.5635106583982861 1107 10-11 0.19616096548048034 1107 12-13 0.6149760764442931 1107 14-15 0.4009549476893426 1107 16-17 1.1890983184435484 1107 18-19 0.9621726370120385 1107 20-21 0.8662912167643384 1107 22-23 0.5745432227779759 1107 24-25 0.801747370346888 1107 26-27 0.8319954934817062 1107 28-29 0.6994718765089161 1107 30-31 0.23825376769468676 1107 32-33 0.7870414705114541 1107 34-35 0.7298462728005646 1107 36-37 0.6918523117174189 1107 38-39 0.3866930375963591 1107 40-41 0.779181565245274 1107 42-43 0.6929120374098048 1107 44-45 0.4048167356271364 1107 46-47 0.5096791312536411 1107 48-49 0.8267350346458926 1107 50-51 0.9410689973878803 1107 52-53 0.9484362232348076 1107 54-55 0.7520873924291323 1107 56-57 0.7877016554836374 1107 58-59 0.3094339713794909 1107 60-61 0.5649678792331798 1107 62-63 0.7133035601786588 1107 64-65 0.788772376691476 1107 66-67 0.5491936129903223 1107 68-69 0.8332044874112228 1107 70-71 0.6603617525514451 1107 72-73 0.5893314783037198 1107 74-75 0.6302919463846735 1107 76 0.567833614948043 1108 1 -0.3673116534965999 1108 2 0.020605072158922155 1108 3 -0.44811498498868474 1108 4 0.23091268956344635 1108 5 0.17787579332023995 1108 6 0.18827079249154366 1108 7 0.025759759670705762 1108 8 -0.05135852399680374 1108 9 -0.2855689327003006 1108 10-11 0.21315093330900226 1108 12-13 -0.22199637791598548 1108 14-15 0.09254340656758586 1108 16-17 0.19453954997032952 1108 18-19 0.05623138327614896 1108 20-21 -0.3642012543479538 1108 22-23 0.19746746433692408 1108 24-25 -0.039438469125670395 1108 26-27 -0.13268250617564092 1108 28-29 0.23947682596732278 1108 30-31 0.1698592324044057 1108 32-33 0.0672950350733359 1108 34-35 0.24878429669756486 1108 36-37 -0.04930687682522006 1108 38-39 0.0790017061285937 1108 40-41 0.40084940582194406 1108 42-43 0.3078600363691564 1108 44-45 0.07118199296724015 1108 46-47 0.1760475613235073 1108 48-49 -0.01566278759240447 1108 50-51 0.17035900392371417 1108 52-53 -0.02701319393474222 1108 54-55 0.5106585218574722 1108 56-57 0.16803263465148888 1108 58-59 0.19877867577626063 1108 60-61 0.5702599924615583 1108 62-63 0.34834762939492947 1108 64-65 0.5362928883893332 1108 66-67 0.20038370392075677 1108 68-69 0.10809931141990603 1108 70-71 0.12866300958697252 1108 72-73 -0.07329313065733345 1108 74-75 0.10034772297373706 1108 76 0.09599159150791792 1109 1 0.4852061747398295 1109 2 0.46243908362149355 1109 3 0.2559956657028799 1109 4 0.4394639930090918 1109 5 0.2634450781282709 1109 6 0.6894426748158082 1109 7 0.179923223192354 1109 8 0.340137672854425 1109 9 0.6647370067118175 1109 10-11 0.6973758347146344 1109 12-13 0.3619197715599469 1109 14-15 0.5171710604904263 1109 16-17 0.7417585950893653 1109 18-19 0.438458772197329 1109 20-21 0.374259611032457 1109 22-23 0.4591784750307468 1109 24-25 0.0442620540026013 1109 26-27 0.05567492105827654 1109 28-29 0.32267109593151844 1109 30-31 0.19265312486623998 1109 32-33 0.3942752036993866 1109 34-35 0.2757625903118992 1109 36-37 0.33777054240865567 1109 38-39 0.36404098762410086 1109 40-41 0.32953646011603865 1109 42-43 0.054617828605351804 1109 44-45 0.15427626230642488 1109 46-47 0.38852701432008985 1109 48-49 0.2606920646254167 1109 50-51 0.3184724296326209 1109 52-53 0.23573844826302093 1109 54-55 0.3223010946235547 1109 56-57 0.006397249430335705 1109 58-59 0.3259638456835674 1109 60-61 0.041387937011208464 1109 62-63 0.35018201585985054 1109 64-65 0.40610769973189775 1109 66-67 0.6935430361665809 1109 68-69 0.43232633783882335 1109 70-71 0.5453031135876216 1109 72-73 0.3047842323261847 1109 74-75 0.3339116827484858 1109 76 -0.04283427615375501 1110 1 -0.1732616027440841 1110 2 -0.0800540839708539 1110 3 -0.08401894368491014 1110 4 -0.12884119206414368 1110 5 0.2751451826609568 1110 6 0.21955564568519748 1110 7 -0.39568035862651385 1110 8 -0.2729402480785623 1110 9 -0.2359254534587052 1110 10-11 -0.10705358053202474 1110 12-13 -0.17683603756084665 1110 14-15 -0.012633350033368629 1110 16-17 -0.30983080554791087 1110 18-19 -0.09386134971548898 1110 20-21 -0.17332480226208702 1110 22-23 0.05647258971443847 1110 24-25 -0.07563253170791029 1110 26-27 -0.08059225441810014 1110 28-29 -0.06129761982773374 1110 30-31 0.11242743501346553 1110 32-33 0.21414837910020168 1110 34-35 -0.04293301805575567 1110 36-37 0.15462738300259815 1110 38-39 -0.300154047702911 1110 40-41 -0.1335244585121842 1110 42-43 0.04073576162887349 1110 44-45 -0.4495920768832562 1110 46-47 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-0.2553715997133601 2116 5 -0.5677601169041999 2116 6 0.1533580742870555 2116 7 0.499575221017345 2116 8 0.3700878600430926 2116 9 -0.12596793519406901 2116 10-11 0.49369956173792673 2116 12-13 -0.30054999387608206 2116 14-15 0.1922940300089877 2116 16-17 0.231946033760849 2116 18-19 0.16720395185470238 2116 20-21 0.17943964340765461 2116 22-23 0.2810086114690975 2116 24-25 0.25233210444041276 2116 26-27 0.27130751876201487 2116 28-29 -0.18446332365861196 2116 30-31 0.015245766070250966 2116 32-33 -0.05489947864237621 2116 34-35 0.3061409051763633 2116 36-37 -0.2600300688451753 2116 38-39 0.11890195550515159 2116 40-41 -0.31860196574912436 2116 42-43 0.04850841542153006 2116 44-45 0.3454962074310828 2116 46-47 0.1473692570841152 2116 48-49 0.10902549170933185 2116 50-51 0.03818942786386259 2116 52-53 -0.14920770765045432 2116 54-55 -0.21003973250661545 2116 56-57 0.11940420572381072 2116 58-59 -0.026907109759903847 2116 60-61 -0.06315646639130534 2116 62-63 -0.08930823095419527 2116 64-65 -0.33777194951590417 2116 66-67 -0.4691923559296214 2116 68-69 -0.1674617858369558 2116 70-71 0.17729143851465778 2116 72-73 -0.36755746980945503 2116 74-75 -0.16773207752501662 2116 76 0.4151935865203882 2117 1 -0.5156968180362291 2117 2 -0.6522409989378453 2117 3 -0.1387780332503823 2117 4 -0.318699798823431 2117 5 -0.3931965258817556 2117 6 -0.23669620178745987 2117 7 -0.22985121289787003 2117 8 -0.24308508993490108 2117 9 -0.7539567865949763 2117 10-11 -0.275746308879647 2117 12-13 -0.6017485905217299 2117 14-15 -0.335134335352123 2117 16-17 -0.6411011277471417 2117 18-19 -0.044946431989608016 2117 20-21 -0.8807713487198612 2117 22-23 -0.5089772082952138 2117 24-25 -0.3773987155683898 2117 26-27 -0.16342061189174473 2117 28-29 -0.7867016005154852 2117 30-31 -0.31379682353417593 2117 32-33 -0.7596113509224622 2117 34-35 -0.215493122499538 2117 36-37 -0.2786049551587979 2117 38-39 -0.19932514830395576 2117 40-41 -0.4163355729076983 2117 42-43 -0.43625088730205874 2117 44-45 -0.7965659166704313 2117 46-47 -0.44276997816111674 2117 48-49 -0.36659305995073055 2117 50-51 -0.4763086406902417 2117 52-53 -0.3552795378788005 2117 54-55 -0.61669160772054 2117 56-57 -0.653109974511878 2117 58-59 -0.6048097795559997 2117 60-61 -0.7236865138217539 2117 62-63 -0.2700634507478483 2117 64-65 -0.6694580528849272 2117 66-67 -0.44103053010203297 2117 68-69 -0.7271532434673915 2117 70-71 -0.4395671503074112 2117 72-73 -1.313452536065384 2117 74-75 -0.7983583305199744 2117 76 -0.8577576139099108 2118 1 0.6529425889460754 2118 2 0.052768565863686945 2118 3 0.4574199581412941 2118 4 0.012732530205742165 2118 5 0.4360717668011702 2118 6 0.7093583175812626 2118 7 0.433563421248472 2118 8 0.05734532613396226 2118 9 0.37229859360588335 2118 10-11 -0.21532665321250022 2118 12-13 0.20014643578146973 2118 14-15 0.4512776684738071 2118 16-17 0.5609254145771061 2118 18-19 0.051867293500585276 2118 20-21 0.33358780957760104 2118 22-23 0.6812427821399822 2118 24-25 0.6513315570284561 2118 26-27 0.09001076065727176 2118 28-29 0.3300248461607467 2118 30-31 0.4846728082209637 2118 32-33 0.3225562722243538 2118 34-35 0.18490740356454438 2118 36-37 0.22143091284694094 2118 38-39 0.28683577948179106 2118 40-41 0.38144061073649027 2118 42-43 0.6613878023201281 2118 44-45 0.2646069671172313 2118 46-47 0.29493327387141477 2118 48-49 0.3686173656829368 2118 50-51 0.3546574042643229 2118 52-53 0.19549760224553836 2118 54-55 0.2623554989269934 2118 56-57 0.5752675960285316 2118 58-59 0.6838738646333837 2118 60-61 0.1363326157813063 2118 62-63 0.554338749156507 2118 64-65 0.6326282694488796 2118 66-67 0.5150969208783636 2118 68-69 0.6101733945048764 2118 70-71 0.3065232370670543 2118 72-73 0.30880953949655066 2118 74-75 0.34824018693578296 2118 76 -9.307368176081354E-4 2119 1 -0.1277867034953637 2119 2 -0.10300298690134824 2119 3 -0.27957593657012936 2119 4 -0.3453693775460067 2119 5 0.12413020679151288 2119 6 -0.5430444836744783 2119 7 -0.2001482426008394 2119 8 -0.28356266710438405 2119 9 0.07394070903693972 2119 10-11 -0.19788521394661984 2119 12-13 -0.21945631717674274 2119 14-15 -0.060029986681961134 2119 16-17 0.005775692287798506 2119 18-19 -0.3627366668954508 2119 20-21 -0.23169835318502408 2119 22-23 -0.33937421858448147 2119 24-25 -0.007494813413465806 2119 26-27 0.031347394320640376 2119 28-29 0.14345759187434481 2119 30-31 -0.37752231373025325 2119 32-33 0.2147018892250827 2119 34-35 -0.1965040912434759 2119 36-37 -0.2712520139823269 2119 38-39 -0.221978516581963 2119 40-41 -0.015782282284519056 2119 42-43 -0.2099234751490755 2119 44-45 -0.0036494926750805234 2119 46-47 -0.045286479811274205 2119 48-49 -0.06801026049431158 2119 50-51 -0.12455655371684315 2119 52-53 -0.2355697727846433 2119 54-55 -0.06426246774772437 2119 56-57 -0.2329852414503364 2119 58-59 -0.27986074053445265 2119 60-61 -0.21679464816459415 2119 62-63 0.01580337122846487 2119 64-65 0.003287530496933755 2119 66-67 -0.16015060387411495 2119 68-69 -0.4972221621239541 2119 70-71 -0.5591070454733966 2119 72-73 -0.1593805112158435 2119 74-75 -0.48823650951435127 2119 76 0.11600961575422986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 35.0 13 410.0 14 1258.0 15 1991.0 16 2539.0 17 3024.0 18 3233.0 19 2797.0 20 2156.0 21 1596.0 22 1169.0 23 1067.0 24 951.0 25 1047.0 26 1016.0 27 1258.0 28 1464.0 29 1775.0 30 2217.0 31 2768.0 32 3373.0 33 4706.0 34 6466.0 35 10199.0 36 19286.0 37 74570.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.49114010448114 21.007140419499645 14.033418213320031 27.468301262699185 2 19.134749166513533 22.24818208069724 33.03494080277216 25.582127950017064 3 17.985587903289318 24.559630378284723 28.913448665109076 28.54133305331688 4 14.47181896936445 19.642716509594937 32.6818575591316 33.20360696190901 5 14.570262252907359 33.331583230514795 33.988528076024465 18.109626440553384 6 27.768225133226576 33.68007245425669 19.815976688630457 18.73572572388628 7 26.37295566114509 28.911479799438215 21.914131205208307 22.801433334208383 8 23.317932428530174 31.71055049484157 22.097235712598113 22.874281364030136 9 23.08757514503977 17.811671435696848 22.231118578216467 36.86963484104691 10-11 25.32026881579293 24.317788045047646 25.95457170608773 24.40737143307169 12-13 24.59441367180322 21.943992334549655 25.274656761084714 28.186937232562414 14-15 23.181096264405536 23.133187199747983 23.5105531199958 30.17516341585068 16-17 23.92335862231906 25.046596487543642 24.256425064972568 26.77361982516473 18-19 22.9858504187121 24.335835980363846 25.52142125849894 27.156892342425117 20-21 23.133843488304937 24.483172761399732 25.516170950043314 26.866812800252017 22-23 24.3128658808705 24.43985771664085 24.66726170162497 26.580014700863675 24-25 23.445580552857482 25.026579686556584 24.408355865907122 27.119483894678815 26-27 22.944832383902554 25.14175832830179 25.42691570629774 26.48649358149791 28-29 23.540742393615623 24.57177171658835 26.591499750610346 25.29598613918568 30-31 23.46173661084914 24.123277647066544 26.194859274864395 26.220126467219917 32-33 22.713490667576718 25.07645761688499 25.986073556821466 26.223978158716825 34-35 23.12505947634564 24.355932703950543 26.52891124652576 25.990096573178057 36-37 23.441314677237287 23.866917806421128 26.199039193552622 26.492728322788967 38-39 22.452287821909536 23.98439345811566 27.786273068542776 25.777045651432022 40-41 22.305607329430604 23.528601055312 28.070446013703304 26.095345601554094 42-43 21.282125324862836 23.736316383587535 28.059617252513586 26.921941039036046 44-45 20.61828944950516 23.50267765731237 29.11033523219489 26.768697660987584 46-47 20.80664426535059 24.016879741684825 29.168088625206728 26.008387367757855 48-49 20.6648859370488 24.454624209172287 28.88063423726144 25.99985561651747 50-51 20.421402882419343 25.06562885569527 27.964783556033918 26.54818470585147 52-53 19.578728375292048 24.979983199012942 28.485876670254378 26.955411755440633 54-55 19.439267056939595 25.109928333289584 28.17971805843593 27.27108655133489 56-57 19.71654897225212 25.04594019898669 28.12426167537343 27.113249153387763 58-59 20.162168902423016 24.80081642296485 27.484708476623005 27.55230619798913 60-61 20.20187436011866 24.07922715459533 27.771834720289817 27.947063764996194 62-63 19.890137295566117 24.456264930564668 27.41218859107973 28.24140918278949 64-65 20.955949912057335 24.51861234347518 26.479274407371435 28.046163337096054 66-67 20.828958076286984 24.93502743286168 25.63364660173785 28.602367889113484 68-69 20.956278056335808 25.38195994014648 25.562111149030002 28.099650854487702 70-71 21.8170720902531 25.086384264773926 26.094774941508092 27.001768703464883 72-73 22.744336229753497 24.73420313443415 25.66514845247158 26.85631218334077 74-75 22.04637334943428 26.01002808915024 25.795421731026696 26.148176830388785 76 22.13989446880004 25.641193920142808 26.011340666264143 26.20757094479301 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.0 11 2.5 12 3.0 13 2.0 14 3.0 15 9.0 16 12.0 17 11.0 18 13.5 19 18.0 20 31.0 21 42.0 22 61.0 23 98.5 24 139.5 25 162.0 26 261.5 27 436.0 28 637.0 29 763.0 30 915.5 31 1270.0 32 1814.0 33 2156.0 34 2418.0 35 3020.5 36 3715.5 37 4070.0 38 4613.0 39 5818.5 40 7043.5 41 7785.5 42 7965.0 43 8186.0 44 8900.0 45 9334.0 46 9275.0 47 9337.0 48 8974.0 49 8094.5 50 7640.0 51 7093.5 52 5932.0 53 4834.0 54 4351.0 55 4261.5 56 3834.5 57 3317.0 58 3137.0 59 3005.5 60 2899.5 61 2938.5 62 2952.0 63 2961.0 64 2950.0 65 2862.0 66 2750.5 67 2707.0 68 2512.0 69 2123.5 70 1728.0 71 1526.0 72 1337.5 73 939.0 74 559.0 75 389.0 76 276.0 77 111.0 78 41.5 79 24.0 80 20.0 81 14.5 82 10.5 83 8.0 84 8.0 85 6.0 86 2.5 87 1.0 88 4.0 89 5.5 90 3.5 91 5.0 92 7.0 93 4.5 94 2.0 95 3.0 96 4.0 97 5.0 98 6.5 99 20.5 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 3.2814427847636046E-4 32-33 0.0 34-35 3.2814427847636046E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 3.2814427847636046E-4 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 152372.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.58596067518967 #Duplication Level Percentage of deduplicated Percentage of total 1 72.80465401102266 39.0130732680545 2 12.716472749540722 13.628488173680203 3 5.167176974892835 8.30664426535059 4 2.5486834047764853 5.462945948074449 5 1.5480710349050826 4.147743679941197 6 1.020208205756277 3.2801302076496994 7 0.8952847519902021 3.358228545927073 8 0.6417636252296387 2.751161630745806 9 0.47274954072259645 2.2799464468537525 >10 2.17636252296387 17.507153545270786 >50 0.008573178199632577 0.26448428845194655 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005906597012574489 0.0 2 0.0 0.0 0.0 0.009844328354290815 0.0 3 0.0 0.0 0.0 0.009844328354290815 0.0 4 0.0 0.0 0.0 0.05709710445488672 0.0 5 0.0 0.0 0.0 0.06431627858136665 0.0 6 0.0 0.0 0.0 0.06628514425222481 0.0 7 0.0 0.0 0.0 0.06694143280917754 0.0 8 0.0 0.0 0.0 0.06759772136613026 0.0 9 0.0 0.0 0.0 0.07219174126479931 0.0 10 0.0 0.0 0.0 0.0748168954926102 0.0 11 0.0 0.0 0.0 0.07678576116346836 0.0 12 0.0 0.0 0.0 0.07678576116346836 0.0 13 0.0 0.0 0.0 0.07678576116346836 0.0 14 0.0 0.0 0.0 0.0780983382773738 0.0 15 0.0 0.0 0.0 0.07941091539127924 0.0 16 0.0 0.0 0.0 0.08334864673299557 0.0 17 0.0 0.0 0.0 0.08400493528994829 0.0 18 0.0 0.0 0.0 0.08728637807471189 0.0 19 0.0 0.0 0.0 0.09188039797338093 0.0 20 0.0 0.0 0.0 0.09516184075814454 0.0 21 0.0 0.0 0.0 0.09975586065681359 0.0 22 0.0 0.0 0.0 0.10303730344157719 0.0 23 0.0 0.0 0.0 0.10566245766938807 0.0 24 0.0 0.0 0.0 0.11156905468196257 0.0 25 0.0 6.56288556952721E-4 0.0 0.12075709447930066 0.0 26 0.0 6.56288556952721E-4 0.0 0.1312577113905442 0.0 27 0.0 6.56288556952721E-4 0.0 0.20738718399705983 0.0 28 0.0 6.56288556952721E-4 0.0 0.28745438794529177 0.0 29 0.0 6.56288556952721E-4 0.0 0.3793347859186727 0.0 30 0.0 6.56288556952721E-4 0.0 0.5164990943217914 0.0 31 0.0 6.56288556952721E-4 0.0 0.6516945370540519 0.0 32 0.0 6.56288556952721E-4 0.0 0.7809833827737379 0.0 33 0.0 6.56288556952721E-4 0.0 0.9627753130496417 0.0 34 0.0 6.56288556952721E-4 0.0 1.2416979497545482 0.0 35 0.0 6.56288556952721E-4 0.0 1.5809991336991047 0.0 36 0.0 6.56288556952721E-4 0.0 2.0554957603759223 0.0 37 0.0 6.56288556952721E-4 0.0 2.7741317302391515 0.0 38 0.0 6.56288556952721E-4 0.0 3.5111437796970573 0.0 39 0.0 6.56288556952721E-4 0.0 4.156931719738535 0.0 40 0.0 6.56288556952721E-4 0.0 4.9444779880817995 0.0 41 0.0 6.56288556952721E-4 0.0 5.780589609639566 0.0 42 0.0 6.56288556952721E-4 0.0 6.615388654083428 0.0 43 0.0 6.56288556952721E-4 0.0 7.456094295539863 0.0 44 0.0 6.56288556952721E-4 0.0 8.280392723072481 0.0 45 0.0 6.56288556952721E-4 0.0 9.094190533693855 0.0 46 0.0 6.56288556952721E-4 0.0 9.926364423909904 0.0 47 0.0 6.56288556952721E-4 0.0 10.7454125429869 0.0 48 0.0 6.56288556952721E-4 0.0 11.475861706875278 0.0 49 0.0 6.56288556952721E-4 0.0 12.217467776231853 0.0 50 0.0 6.56288556952721E-4 0.0 12.880975507311055 0.0 51 0.0 6.56288556952721E-4 0.0 13.59370488016171 0.0 52 0.0 6.56288556952721E-4 0.0 14.355655894783819 0.0 53 0.0 6.56288556952721E-4 0.0 15.08938650145696 0.0 54 0.0 6.56288556952721E-4 0.0 15.797521854408947 0.0 55 0.0 6.56288556952721E-4 0.0 16.419683406400125 0.0 56 0.0 6.56288556952721E-4 0.0 17.064158769327697 0.0 57 0.0 6.56288556952721E-4 0.0 17.68435145564802 0.0 58 0.0 6.56288556952721E-4 0.0 18.25138476885517 0.0 59 0.0 6.56288556952721E-4 0.0 18.87879662930197 0.0 60 0.0 6.56288556952721E-4 0.0 19.460924579319034 0.0 61 0.0 6.56288556952721E-4 0.0 20.0069566587037 0.0 62 0.0 6.56288556952721E-4 0.0 20.640275116163075 0.0 63 0.0 6.56288556952721E-4 0.0 21.258498936812536 0.0 64 0.0 6.56288556952721E-4 0.0 21.86031554353818 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGAGTC 15 0.002221192 70.0 6 CAGCGAA 20 0.006940526 52.5 38 GAAGCAA 20 0.006940526 52.5 47 ACGTGTC 20 0.006940526 52.5 31 ACGGAAG 20 0.006940526 52.5 44 AAGGGTT 20 0.006940526 52.5 43 GACGAAT 20 0.006940526 52.5 23 CGGGCCA 30 5.863271E-4 46.666664 5 CTGAGAC 35 0.0012523439 40.0 47 TGTATAG 40 0.0024128745 35.0 49 TAGATCT 65 2.0921374E-5 32.307693 39 AACCGAT 50 0.0071910606 27.999998 28 CCGATAC 50 0.0071910606 27.999998 39 ACCGATA 50 0.0071910606 27.999998 29 TACGGTT 50 0.0071910606 27.999998 43 CTATAAA 70 0.001221676 25.0 1 GGTTGTA 100 1.3884348E-5 24.499998 46 CGACGAA 75 0.0018257763 23.333334 22 GAATCGT 195 3.092282E-11 23.333334 70 ATATCGC 75 0.0018257763 23.333334 33 >>END_MODULE