FastQCFastQC Report
Wed 25 May 2016
SRR1780320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152372
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT93406.129735121938413No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC29631.9445829942509123No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG22761.493712755624393No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG21241.3939568949675794Illumina PCR Primer Index 8 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5530.3629275719948547No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2790.18310450738980916RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT2450.16079069645341665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCT150.00222119270.033
TGTCATC252.384437E-455.99999654
GCCATAG252.384437E-455.9999961
CTTTATA509.731593E-1055.9999962
AGATAAT200.00694052652.532
TTGAACG200.00694052652.54
AGTTATC200.00694052652.532
TATGTCA200.00694052652.552
TTAATAC352.0788782E-550.03
GTCTGAT305.863271E-446.66666425
GTAGCAG305.863271E-446.66666455
TCTTTAT802.910383E-1143.751
CTTAATA503.4609766E-642.02
ATCGTAT350.001252343940.039
TATCTTC350.001252343940.066
TTCTTGT350.001252343940.012
CCATAGA350.001252343940.02
CCCCAGT350.001252343940.017
TCTTTCA350.001252343940.010
ATAACAT350.001252343940.031