##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780320_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 152372 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76817263014202 34.0 34.0 34.0 34.0 34.0 2 33.767923240490376 34.0 34.0 34.0 34.0 34.0 3 33.77314073451815 34.0 34.0 34.0 34.0 34.0 4 33.79037487202373 34.0 34.0 34.0 34.0 34.0 5 33.7756936970047 34.0 34.0 34.0 34.0 34.0 6 37.552680282466596 38.0 38.0 38.0 37.0 38.0 7 37.53564959441367 38.0 38.0 38.0 37.0 38.0 8 37.474286614338595 38.0 38.0 38.0 37.0 38.0 9 37.492045782689736 38.0 38.0 38.0 37.0 38.0 10-11 37.43339655579766 38.0 38.0 38.0 37.0 38.0 12-13 37.412992544561995 38.0 38.0 38.0 37.0 38.0 14-15 37.33552096185651 38.0 38.0 38.0 37.0 38.0 16-17 37.37515422781088 38.0 38.0 38.0 36.5 38.0 18-19 37.3481643609062 38.0 38.0 38.0 36.5 38.0 20-21 37.35852059433492 38.0 38.0 38.0 37.0 38.0 22-23 37.35389047856562 38.0 38.0 38.0 37.0 38.0 24-25 37.36344932139763 38.0 38.0 38.0 37.0 38.0 26-27 37.2701579030268 38.0 38.0 38.0 36.0 38.0 28-29 37.2376158349303 38.0 38.0 38.0 36.0 38.0 30-31 37.23601120940855 38.0 38.0 38.0 36.0 38.0 32-33 37.170822067046444 38.0 38.0 38.0 36.0 38.0 34-35 37.179996981072634 38.0 38.0 38.0 36.0 38.0 36-37 37.20232063633738 38.0 38.0 38.0 36.0 38.0 38-39 36.695134276638754 38.0 37.5 38.0 34.0 38.0 40-41 36.93832200141758 38.0 37.5 38.0 35.5 38.0 42-43 37.13557280865251 38.0 38.0 38.0 36.0 38.0 44-45 37.20922479195653 38.0 38.0 38.0 36.0 38.0 46-47 37.15498910560996 38.0 38.0 38.0 36.0 38.0 48-49 37.17573110545244 38.0 38.0 38.0 36.0 38.0 50-51 37.056316121072115 38.0 38.0 38.0 36.0 38.0 52-53 37.064500039377315 38.0 38.0 38.0 36.0 38.0 54-55 37.02526382799989 38.0 38.0 38.0 36.0 38.0 56-57 37.069205628330664 38.0 38.0 38.0 36.0 38.0 58-59 36.9731643609062 38.0 38.0 38.0 36.0 38.0 60-61 36.98580447851312 38.0 38.0 38.0 36.0 38.0 62-63 36.97082141075788 38.0 38.0 38.0 36.0 38.0 64-65 36.90899902869293 38.0 38.0 38.0 35.5 38.0 66-67 36.61953311632058 38.0 37.5 38.0 34.5 38.0 68-69 36.53680466227391 38.0 38.0 38.0 34.0 38.0 70-71 35.90227863386974 38.0 37.0 38.0 31.5 38.0 72-73 34.84563108707636 38.0 37.0 38.0 24.0 38.0 74-75 34.49984249074633 38.0 37.0 38.0 23.0 38.0 76 33.92408710261728 38.0 35.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06194774170506889 1101 2 0.03642068340068505 1101 3 -0.08877050184090507 1101 4 -0.04504792784942424 1101 5 -0.15310732522434733 1101 6 -0.17149906475694365 1101 7 0.023151907214462142 1101 8 -0.026887201206683642 1101 9 0.06783791357365487 1101 10-11 0.10163451331934681 1101 12-13 0.18020560651946482 1101 14-15 0.19271454433632584 1101 16-17 0.2030003682719368 1101 18-19 0.055508273579413014 1101 20-21 0.058989023816373276 1101 22-23 -0.046788729080695646 1101 24-25 -0.022096453660992665 1101 26-27 0.12711090697263216 1101 28-29 -0.30127156954358014 1101 30-31 -0.049376136903518386 1101 32-33 0.05237485975958123 1101 34-35 -0.013114774066217194 1101 36-37 0.09941987723199475 1101 38-39 0.22098542667074383 1101 40-41 -0.10750178190842519 1101 42-43 0.09507932946694808 1101 44-45 0.10850651009562284 1101 46-47 0.0952060718368557 1101 48-49 0.11350537799535942 1101 50-51 0.15755836009108037 1101 52-53 -0.006551233544243473 1101 54-55 -0.049599093351936574 1101 56-57 0.018904605545934317 1101 58-59 -0.09031593525502046 1101 60-61 -0.052401575843965986 1101 62-63 0.1796890249158949 1101 64-65 -0.11554872377614345 1101 66-67 -0.32807553198600203 1101 68-69 -0.5832831263311249 1101 70-71 -0.16162348663394255 1101 72-73 0.14866653960957876 1101 74-75 0.021242857420638472 1101 76 -1.0661607669344946 1102 1 -0.02688824805151313 1102 2 0.03241564458262047 1102 3 -0.1680155006770434 1102 4 -0.09042467534712983 1102 5 -0.009268238783974425 1102 6 -0.07809608254342493 1102 7 -0.17634694946676177 1102 8 -0.1402195304277214 1102 9 -0.12203515750380944 1102 10-11 0.009148924612873088 1102 12-13 0.013866417385784757 1102 14-15 -0.0443324070418214 1102 16-17 -0.03409792975771353 1102 18-19 -0.08949809340537485 1102 20-21 -0.06572172338010063 1102 22-23 0.12573944311543528 1102 24-25 -0.0892510276207048 1102 26-27 0.04092041774173083 1102 28-29 -0.046301213677061526 1102 30-31 -6.926650483265462E-4 1102 32-33 -0.12583225209396431 1102 34-35 0.056216897917686026 1102 36-37 0.15019486935884174 1102 38-39 0.08947980592395055 1102 40-41 0.08182016844278905 1102 42-43 0.03117671010309664 1102 44-45 0.2425863096382983 1102 46-47 -0.04280003499156493 1102 48-49 0.03290140444915579 1102 50-51 -0.03878438907710091 1102 52-53 -0.08093541603709298 1102 54-55 0.040569049816220115 1102 56-57 -0.06881943701016979 1102 58-59 -0.05197710212479478 1102 60-61 0.06333377666791051 1102 62-63 0.03899235354815289 1102 64-65 -0.23637081789797776 1102 66-67 -0.33569195196053414 1102 68-69 -0.4633031172941102 1102 70-71 -0.635994661308402 1102 72-73 -0.40087955599103964 1102 74-75 -0.346830479290162 1102 76 -0.4235540001304514 1103 1 0.002999999154667421 1103 2 -0.05784911203629406 1103 3 -0.08707597718895954 1103 4 -0.022747983429830754 1103 5 0.02059500779719059 1103 6 -7.31648432569898E-4 1103 7 0.08622211475984187 1103 8 0.13747491193333872 1103 9 0.19511027113190949 1103 10-11 0.16636535502363614 1103 12-13 0.14027582762103918 1103 14-15 0.23014320484847417 1103 16-17 0.044616538103980474 1103 18-19 0.180252400356963 1103 20-21 0.11040767985497979 1103 22-23 0.24167359146914436 1103 24-25 0.14304852986822425 1103 26-27 0.17183619063605704 1103 28-29 0.2563626029183581 1103 30-31 0.04287717419765613 1103 32-33 0.15605604511345916 1103 34-35 0.031403777589680715 1103 36-37 0.22879683440797294 1103 38-39 0.017015494316162005 1103 40-41 0.20073564132960797 1103 42-43 0.03688935291916806 1103 44-45 0.02655590887827941 1103 46-47 -0.0013614990281638484 1103 48-49 0.19496795611294715 1103 50-51 -0.03306549610478271 1103 52-53 -0.06702881837215102 1103 54-55 0.19667295241378469 1103 56-57 -0.024105819169726317 1103 58-59 0.2784499085504706 1103 60-61 0.16840515345233342 1103 62-63 0.01483549962681252 1103 64-65 0.24434119990246472 1103 66-67 -0.014971433947586377 1103 68-69 -0.003254116069079771 1103 70-71 0.05062250412073155 1103 72-73 0.24089273831631886 1103 74-75 0.44430804917304556 1103 76 0.6841611927493716 1104 1 -0.12287309841168792 1104 2 0.0014479984702830961 1104 3 0.041529762585078345 1104 4 0.04605823179944224 1104 5 0.025239274150159474 1104 6 0.12190639288792227 1104 7 0.057903118607598 1104 8 -0.14202659530877781 1104 9 -0.14177672247150497 1104 10-11 -0.012028390534290168 1104 12-13 -0.027677509143416046 1104 14-15 -0.08699646625413493 1104 16-17 0.08113958787973274 1104 18-19 0.04813588931610724 1104 20-21 0.008436011105125374 1104 22-23 -0.15329736654864945 1104 24-25 -0.15509131279039678 1104 26-27 -0.07051731278281181 1104 28-29 -0.057774837956081626 1104 30-31 0.02770672104470151 1104 32-33 -0.2190879393974825 1104 34-35 -0.08282189209147361 1104 36-37 0.001662800145780352 1104 38-39 -0.08234607223531754 1104 40-41 -0.009046727613849725 1104 42-43 -0.18411155387690314 1104 44-45 0.0315310332563854 1104 46-47 -0.10113357461084149 1104 48-49 -0.09936089225604405 1104 50-51 -0.2577548571811761 1104 52-53 -0.095268163518341 1104 54-55 -0.18847642385757268 1104 56-57 -0.3386476420749034 1104 58-59 -0.11930883467918818 1104 60-61 -0.19903719147328758 1104 62-63 -0.36721974140939295 1104 64-65 -0.25609381713059065 1104 66-67 -0.48012718229942664 1104 68-69 -0.46852627742976694 1104 70-71 -0.30092027459652115 1104 72-73 -0.4501777712050341 1104 74-75 -0.5387630485403605 1104 76 -0.316157411274844 1105 1 -0.0718207025317028 1105 2 0.07574266931668205 1105 3 0.050172843641952625 1105 4 0.03186208312053651 1105 5 0.15367635431137217 1105 6 0.0859905845583242 1105 7 0.06963621309931511 1105 8 -0.14426573547895316 1105 9 -0.02099750169227832 1105 10-11 -0.0133270918329913 1105 12-13 0.050155003171852286 1105 14-15 -0.05141652895006388 1105 16-17 0.10462816826485977 1105 18-19 0.11166029594397031 1105 20-21 0.2015170679792888 1105 22-23 0.08718628772810177 1105 24-25 0.08380255196553321 1105 26-27 0.13431922998476864 1105 28-29 0.2139448216946036 1105 30-31 -0.005365379268781112 1105 32-33 0.2536169419280867 1105 34-35 0.015095707550266013 1105 36-37 0.04384820095187081 1105 38-39 0.06785545038000151 1105 40-41 0.048409609152827215 1105 42-43 0.0046479624688231524 1105 44-45 -0.001585849860497035 1105 46-47 0.06008451764172662 1105 48-49 -0.022267296196929465 1105 50-51 0.036938380615509914 1105 52-53 0.16635297396486948 1105 54-55 -0.058651076790845025 1105 56-57 0.09113292218842162 1105 58-59 0.26730110710316524 1105 60-61 0.13956558506053085 1105 62-63 0.14077398899813431 1105 64-65 0.17129086715064545 1105 66-67 -0.1915915799707193 1105 68-69 0.022920207120165514 1105 70-71 -0.12512537983610628 1105 72-73 0.04431394397633426 1105 74-75 -0.3065642128932424 1105 76 -0.601423868955095 1106 1 -0.029909527287195203 1106 2 -0.060797333889993865 1106 3 -0.09717363703786219 1106 4 -0.013608495683378408 1106 5 0.10618557348725943 1106 6 1.1596535036773048E-4 1106 7 -0.11840290827313993 1106 8 0.05140526019204117 1106 9 0.07885819816341666 1106 10-11 -0.16652574503164175 1106 12-13 -0.05019773051421339 1106 14-15 -0.37280501700521995 1106 16-17 0.0464591767625393 1106 18-19 0.020735171685508647 1106 20-21 0.03775243754798652 1106 22-23 -0.17874284486769199 1106 24-25 -0.024935623439304777 1106 26-27 -0.24278514711960497 1106 28-29 -0.010377769294649397 1106 30-31 -0.03951641341981116 1106 32-33 0.03141075305524055 1106 34-35 -0.06898317652862573 1106 36-37 -0.06203603826570259 1106 38-39 -0.05120820201698706 1106 40-41 0.01801037875360123 1106 42-43 -0.09161626712874238 1106 44-45 0.1025186875773727 1106 46-47 0.19769875525597058 1106 48-49 0.048984908388604254 1106 50-51 0.009729786740244606 1106 52-53 0.29599863694386386 1106 54-55 0.07407298926656125 1106 56-57 0.09526952632501917 1106 58-59 -0.07646993666787694 1106 60-61 -0.058782979954706605 1106 62-63 -0.1557467741892964 1106 64-65 -0.24950438697794652 1106 66-67 -0.07982310153556682 1106 68-69 -0.14627972874644257 1106 70-71 -0.3056608937049532 1106 72-73 -0.2962824966201083 1106 74-75 -0.3266058996016028 1106 76 -0.3642264984243866 1107 1 0.12509942568613042 1107 2 -0.061457026661642544 1107 3 0.15279337832355822 1107 4 0.12965792595454673 1107 5 0.04097023091822649 1107 6 0.1391415048848117 1107 7 0.1157880256633419 1107 8 0.11349177418695433 1107 9 0.015075331327118136 1107 10-11 0.0021482724973296285 1107 12-13 0.01794853279744757 1107 14-15 0.2557973767371209 1107 16-17 0.12161703181860872 1107 18-19 0.13143161876872256 1107 20-21 0.08057592261066304 1107 22-23 -0.018362107896621183 1107 24-25 0.18020055599712492 1107 26-27 0.26411335443538064 1107 28-29 0.19424950428936683 1107 30-31 -0.0929798004709852 1107 32-33 0.0808500141993278 1107 34-35 0.047867261695863306 1107 36-37 0.15592645348814216 1107 38-39 0.30681648934103833 1107 40-41 0.3267183233394064 1107 42-43 0.1377400612097759 1107 44-45 0.2522689467423902 1107 46-47 0.10332499217991398 1107 48-49 0.08522088991720977 1107 50-51 0.21885239535395584 1107 52-53 0.1783188706635883 1107 54-55 0.37850235501908003 1107 56-57 0.024135797176029428 1107 58-59 0.2650341628514923 1107 60-61 -0.08850685140885872 1107 62-63 0.04856950137379812 1107 64-65 0.17569587689916233 1107 66-67 0.31911196584733403 1107 68-69 0.2569904625644668 1107 70-71 0.12920858136807567 1107 72-73 0.5593861491248759 1107 74-75 0.7755051172676843 1107 76 0.31212822780311456 1108 1 -0.06987750856855968 1108 2 0.0013312951546780027 1108 3 0.12663104877846365 1108 4 0.07641952069543123 1108 5 0.05495562367044471 1108 6 -0.031773446921420145 1108 7 0.09526046415997769 1108 8 0.03178763758189973 1108 9 0.044721318141249355 1108 10-11 0.03446252503393055 1108 12-13 -0.2511275048438222 1108 14-15 0.0183344183569929 1108 16-17 -0.0391293211285344 1108 18-19 0.0431821406827666 1108 20-21 0.08291422443878815 1108 22-23 -0.018701589980217648 1108 24-25 0.08073230550376564 1108 26-27 -0.021495777949944284 1108 28-29 -0.032889391473773344 1108 30-31 -0.11350736197435651 1108 32-33 -0.22093369446793076 1108 34-35 -0.03039446312693883 1108 36-37 0.13884447649677156 1108 38-39 -0.12460495704299746 1108 40-41 0.004146889329007308 1108 42-43 -0.020014407580084992 1108 44-45 -0.1374215851974867 1108 46-47 0.1997030038936316 1108 48-49 -0.04599369448692414 1108 50-51 0.07679976611280637 1108 52-53 -0.11318519360322199 1108 54-55 -0.02597627030057481 1108 56-57 -0.0376943602548252 1108 58-59 -0.169810658936278 1108 60-61 0.02045103377640345 1108 62-63 0.056053970861654534 1108 64-65 0.09638397852804559 1108 66-67 0.12988719063523746 1108 68-69 0.11638459809079649 1108 70-71 0.11279605035464613 1108 72-73 0.042684899703900214 1108 74-75 0.038838514055619555 1108 76 0.3463366000023811 1109 1 0.006329217723475722 1109 2 0.030356476562062085 1109 3 0.03094295072784803 1109 4 -0.03453117274640505 1109 5 0.15426874437744686 1109 6 -0.1369303541520921 1109 7 0.05833523337728508 1109 8 -0.08593809820394682 1109 9 0.024889944195166436 1109 10-11 0.03308439142059427 1109 12-13 0.04789936407411233 1109 14-15 -0.0613504546734589 1109 16-17 0.002645939966846811 1109 18-19 0.043922770311702664 1109 20-21 0.11648630926432446 1109 22-23 -0.2814626072365769 1109 24-25 0.044135201771865695 1109 26-27 0.012491934473260358 1109 28-29 -0.11073050298072218 1109 30-31 -0.010081715564062677 1109 32-33 0.09836517615001128 1109 34-35 -0.04840395068623593 1109 36-37 -0.1376510015999628 1109 38-39 0.06370871991747862 1109 40-41 -0.031084580867670297 1109 42-43 0.09339255121341239 1109 44-45 0.10358617110099999 1109 46-47 0.050970824317509766 1109 48-49 0.02860989678552528 1109 50-51 0.1929192418595278 1109 52-53 -0.03075342840469375 1109 54-55 0.0053270503451798845 1109 56-57 -0.05630385156414519 1109 58-59 0.05725326021321564 1109 60-61 -0.004499122162073377 1109 62-63 -0.23808224248968202 1109 64-65 0.14661554247531683 1109 66-67 0.16238426874197387 1109 68-69 0.1218721323878853 1109 70-71 0.20299036406910886 1109 72-73 -0.0910471861216422 1109 74-75 0.36967183176385277 1109 76 0.5252564682515626 1110 1 -0.021863122423368964 1110 2 -0.030398425769142534 1110 3 0.04806490133300656 1110 4 0.1156054754066389 1110 5 0.1012845250351262 1110 6 -0.37709105741779325 1110 7 0.007070135960375978 1110 8 -0.12765671635737164 1110 9 0.06640705355147247 1110 10-11 -0.1529113768946715 1110 12-13 0.10054983447120946 1110 14-15 -0.005387683423734302 1110 16-17 0.0463040802925363 1110 18-19 0.049949142775524535 1110 20-21 0.008398879634974321 1110 22-23 -0.07149673778909005 1110 24-25 -0.17216560048503027 1110 26-27 -0.20531073803715572 1110 28-29 0.039125922001339575 1110 30-31 -0.04340623288751999 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2115 54-55 0.16972030732626564 2115 56-57 0.08382336133362855 2115 58-59 0.10306461010382861 2115 60-61 0.14959638555587418 2115 62-63 0.19649748310793314 2115 64-65 0.11797425711031906 2115 66-67 0.30992304232630374 2115 68-69 0.24533788723955752 2115 70-71 0.0016710511168014364 2115 72-73 -0.0014803595196823949 2115 74-75 -0.20323966458092002 2115 76 -0.15869056314109997 2116 1 0.08473595776560217 2116 2 0.09360062183797169 2116 3 -0.030476183141736612 2116 4 0.021556677802664126 2116 5 0.05246185808441339 2116 6 0.005633036869099328 2116 7 0.14513577588067506 2116 8 0.056725293442248415 2116 9 0.01978366228090067 2116 10-11 0.040696938999019494 2116 12-13 -0.06783573427025402 2116 14-15 0.04950648818243053 2116 16-17 -0.2274086228741723 2116 18-19 0.09305745240346397 2116 20-21 0.0766798104736992 2116 22-23 -0.0797988468380737 2116 24-25 -0.03148714586779988 2116 26-27 0.06329225197524124 2116 28-29 -0.1887497406009544 2116 30-31 0.1583130869034477 2116 32-33 0.018467801791416605 2116 34-35 -0.020419400782806463 2116 36-37 -0.04694105966282791 2116 38-39 -0.01861991963651377 2116 40-41 -0.2514640832395685 2116 42-43 -0.16091216319105683 2116 44-45 -0.0825587423047125 2116 46-47 -0.09206756967245155 2116 48-49 -0.14075678675624204 2116 50-51 0.2014880454145569 2116 52-53 0.01898438245662959 2116 54-55 0.22340028830291914 2116 56-57 -0.01400358718748862 2116 58-59 0.06959083732307647 2116 60-61 -0.013214801635065498 2116 62-63 0.033610080587337166 2116 64-65 0.0701994398746777 2116 66-67 0.2857475397624185 2116 68-69 0.10765641853966912 2116 70-71 0.4033197411277172 2116 72-73 -0.09571909032104031 2116 74-75 -0.11577495227854939 2116 76 -0.23720454712978523 2117 1 -0.037825286183348794 2117 2 0.026984074969846006 2117 3 0.10366151183650629 2117 4 -0.004603412168492582 2117 5 -0.13791391816125298 2117 6 0.11614719265610773 2117 7 0.1285106719737712 2117 8 -0.447614347651097 2117 9 -0.08715487373200403 2117 10-11 0.15008645540408594 2117 12-13 0.01825724406820939 2117 14-15 0.14218227420756335 2117 16-17 0.04092177661729579 2117 18-19 0.03635790225921909 2117 20-21 0.03111663507885254 2117 22-23 0.02957233305532725 2117 24-25 -0.25662589213938247 2117 26-27 0.15628892695445984 2117 28-29 -0.654322939955513 2117 30-31 -0.06187480121937483 2117 32-33 -0.206597598433504 2117 34-35 -0.05147228328242903 2117 36-37 -0.1843762706549512 2117 38-39 -0.15886978516876837 2117 40-41 -0.24413559074531577 2117 42-43 -0.03195758884261579 2117 44-45 -0.02116504517498896 2117 46-47 -0.053472268709171544 2117 48-49 -0.010292506280961788 2117 50-51 0.1168467075826598 2117 52-53 0.059324708113081215 2117 54-55 0.0047465549891470005 2117 56-57 -0.11064005973161528 2117 58-59 -0.21416056847615295 2117 60-61 -0.4100810917920299 2117 62-63 -0.19391852437085078 2117 64-65 0.038422265164399505 2117 66-67 -0.022397538717854104 2117 68-69 -0.12543731770794153 2117 70-71 -0.4777832611704582 2117 72-73 -0.5351169192780603 2117 74-75 -0.430545623639361 2117 76 -0.7746477039436357 2118 1 -0.08287550139138489 2118 2 -0.13065581025253437 2118 3 -0.33969095109988956 2118 4 -0.01574640595136856 2118 5 -0.016548542742789607 2118 6 -0.06516557635394093 2118 7 0.04233181018515353 2118 8 0.07904753661480868 2118 9 0.047421883800275566 2118 10-11 -0.06133152642279782 2118 12-13 -0.1106463905563686 2118 14-15 0.08132024646963742 2118 16-17 0.052315846440343705 2118 18-19 -0.10091493370443771 2118 20-21 -0.056180112888618794 2118 22-23 -0.04056157416609096 2118 24-25 -0.02302354876300683 2118 26-27 0.013809252157713559 2118 28-29 0.1915343053816514 2118 30-31 0.07990544746546391 2118 32-33 0.0533318611552005 2118 34-35 0.014937329343105432 2118 36-37 -0.02347837491129212 2118 38-39 -0.06391521797603872 2118 40-41 0.253556900885485 2118 42-43 0.11261438628889664 2118 44-45 0.0400676186222384 2118 46-47 0.1766508781105287 2118 48-49 0.1083947247089938 2118 50-51 0.09876923269026605 2118 52-53 0.14655569807003133 2118 54-55 0.0094990657495444 2118 56-57 -0.0787593806307072 2118 58-59 -0.1788186268214389 2118 60-61 -0.03004163698572171 2118 62-63 0.09720414420877432 2118 64-65 -0.006520346026420043 2118 66-67 0.16379327907267793 2118 68-69 0.011865408260483434 2118 70-71 -0.004140267387583663 2118 72-73 0.35638236336182416 2118 74-75 0.7461282166284562 2118 76 0.28840825014531646 2119 1 -0.06767385927594916 2119 2 -0.09612308868769048 2119 3 -0.1004250732925982 2119 4 -0.07109535548046608 2119 5 -0.0822693831195096 2119 6 0.0163364762903484 2119 7 -0.16934411350477774 2119 8 -0.152720152349211 2119 9 -0.0013948271391086564 2119 10-11 0.04949919079526666 2119 12-13 -0.12233438862446633 2119 14-15 -0.6686918279085319 2119 16-17 -0.15794737500375078 2119 18-19 0.055079995056736664 2119 20-21 -0.10481570815546348 2119 22-23 0.006081748702776224 2119 24-25 -0.09834351978449263 2119 26-27 -0.08422262420064897 2119 28-29 -0.08774606167944654 2119 30-31 -0.43751788023315896 2119 32-33 -0.3703158084898064 2119 34-35 -0.07487793267090126 2119 36-37 -0.04065417785743364 2119 38-39 -0.26496568887677796 2119 40-41 -0.07979856880782421 2119 42-43 -0.19420763737687707 2119 44-45 -0.27787490733767584 2119 46-47 0.06731738378548613 2119 48-49 0.022322519927541862 2119 50-51 -0.09522376417039169 2119 52-53 -0.058322350710454884 2119 54-55 -0.045741214376029404 2119 56-57 -0.11099193315425993 2119 58-59 -0.09777540054759726 2119 60-61 0.04998734152189144 2119 62-63 -0.028622950695833538 2119 64-65 -0.40253580123047783 2119 66-67 0.02295773210335028 2119 68-69 -0.24694285061418242 2119 70-71 0.043535414814783735 2119 72-73 -0.2443481365174307 2119 74-75 0.12051242334180756 2119 76 -0.4818404820449729 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 2.0 20 0.0 21 2.0 22 1.0 23 10.0 24 33.0 25 46.0 26 87.0 27 216.0 28 322.0 29 632.0 30 1046.0 31 1691.0 32 2561.0 33 4498.0 34 7516.0 35 12981.0 36 20853.0 37 99873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.89439004541517 17.788701336203502 13.132334024623946 33.18457459375738 2 18.681253773659204 19.434016748483973 37.182684482713356 24.702044995143467 3 17.857611634683536 19.757567007061667 33.07366182763237 29.311159530622422 4 14.02235333495219 23.55960123645576 34.1108216130366 28.307223815555453 5 14.361562491796393 30.812747748930246 39.75139789462631 15.07429186464705 6 27.22153676528496 39.31562229280971 17.389021605019295 16.073819336886043 7 26.09206415876933 26.256136298007508 22.793557871524953 24.85824167169821 8 22.73842963274092 37.34413146772373 17.4388995353477 22.478539364187647 9 21.938413881815556 16.44396608300738 18.695692121912163 42.92192791326491 10-11 24.909104034862047 27.164111516551596 24.868086000052504 23.05869844853385 12-13 24.077258288924476 20.405980101330954 26.700443651064496 28.81631795868007 14-15 21.688696085895046 19.860276166224764 21.6352085685034 36.81581917937679 16-17 26.105189929908384 26.104205497072954 20.98154516577849 26.809059407240177 18-19 27.85354264563043 24.544863885753287 21.87048801617095 25.73110545244533 20-21 23.25164728427795 22.969771349066757 21.56006352873231 32.218517837922974 22-23 22.46541359304859 26.683052004305253 21.37991231984879 29.471622082797367 24-25 26.285997427348857 27.26058593442365 21.942679757435748 24.510736880791747 26-27 27.573307431811617 28.31721051111753 21.760559682881368 22.348922374189485 28-29 22.918580841624443 27.40398498411782 23.920733468091253 25.756700706166484 30-31 21.677211036148375 28.636494894075028 26.279762686057808 23.406531383718793 32-33 24.301052686845352 28.494080277216288 24.411965452970364 22.792901582968 34-35 23.703830100018376 22.957630010763133 30.944005460320795 22.394534428897696 36-37 22.208148478723125 28.90426062531174 26.549497282965373 22.338093612999764 38-39 20.8995090961594 26.04152993988397 27.8637151182632 25.195245845693435 40-41 21.462604678024835 21.15546163337096 29.11230409786575 28.269629590738454 42-43 25.22445068647783 21.00320268815793 28.372338749901555 25.400007875462684 44-45 20.942167852361326 25.3261754128055 30.71102302260258 23.02063371223059 46-47 23.574869398577167 22.527104717402146 28.308350615598666 25.58967526842202 48-49 23.74845772189116 21.52331136954296 25.540453626650567 29.187777281915313 50-51 21.57942404116242 21.910521618145065 28.509503058304674 28.000551282387843 52-53 18.337030425537503 21.886567085816292 34.99560286666842 24.780799621977792 54-55 18.560496679179902 22.621938413881814 31.499553723781272 27.318011183157008 56-57 22.071968603155433 23.445908697135955 26.48419657154858 27.997926128160028 58-59 19.813023390124172 23.61523114482976 31.091670385635155 25.480075079410913 60-61 22.421114115454284 26.965584228073396 26.736211377418424 23.877090279053895 62-63 18.955582390465437 33.74701388706586 22.80996508544877 24.487438637019928 64-65 18.233664977817448 35.654188433570475 21.957774394245664 24.15437219436642 66-67 18.355406505132176 35.20299005066548 21.924959966398024 24.51664347780432 68-69 18.000354395820757 35.77330480665739 21.94563305594204 24.280707741579818 70-71 18.265166828551177 34.81184207072165 22.267214448848872 24.6557766518783 72-73 18.190021788780093 32.82689733021815 22.98060011025648 26.00248077074528 74-75 18.22611765941249 32.207032788176306 23.705142677132283 25.861706875278923 76 18.381986191688764 32.17585908172105 24.122542199354214 25.319612527235975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.5 15 2.0 16 6.0 17 10.0 18 9.5 19 11.5 20 16.0 21 18.0 22 30.0 23 60.0 24 75.0 25 72.0 26 170.0 27 329.5 28 477.0 29 563.0 30 699.0 31 940.0 32 1342.5 33 1640.0 34 1877.5 35 2540.5 36 5322.5 37 7679.0 38 9151.5 39 10463.5 40 9725.5 41 8533.0 42 7918.0 43 7863.0 44 8167.5 45 8438.5 46 8350.0 47 8320.5 48 7929.5 49 7128.0 50 6688.0 51 6223.0 52 5106.0 53 4198.5 54 3943.0 55 3860.0 56 3406.5 57 2906.0 58 2776.0 59 2612.5 60 2489.0 61 2444.0 62 2359.0 63 2437.0 64 2587.5 65 2546.0 66 2464.0 67 2496.0 68 2394.0 69 2125.5 70 1753.0 71 1547.0 72 1343.5 73 928.0 74 544.5 75 373.0 76 251.0 77 84.5 78 25.5 79 11.0 80 7.0 81 1.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 6.562885569527209E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 152372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.43695692121912 #Duplication Level Percentage of deduplicated Percentage of total 1 54.8929550337062 19.45239282807865 2 20.886732350544484 14.803244690625576 3 8.800651900140751 9.35604966791799 4 4.2354989258463585 6.003727719003491 5 2.531669012519446 4.485732286771848 6 1.7982813541743834 3.8235371328065524 7 1.2704644788502852 3.151497650486966 8 0.9537743536558264 2.7039088546452104 9 0.805615230757834 2.5693697004699025 >10 3.7706496777539074 20.929698369779224 >50 0.033335802652048295 0.7462000892552437 >100 0.011111934217349434 0.6497256713831938 >500 0.0018519890362249053 0.3629275719948547 >1k 0.005555967108674717 4.832252644842884 >5k 0.0018519890362249053 6.129735121938413 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 9340 6.129735121938413 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 2963 1.9445829942509123 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 2276 1.493712755624393 No Hit CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG 2124 1.3939568949675794 Illumina PCR Primer Index 8 (95% over 23bp) TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 553 0.3629275719948547 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 279 0.18310450738980916 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT 245 0.16079069645341665 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007875462683432652 0.0 2 0.0 0.0 0.0 0.011813194025148977 0.0 3 0.0 0.0 0.0 0.011813194025148977 0.0 4 0.0 0.0 0.0 0.06497256713831938 0.0 5 0.0 0.0 0.0 0.07284802982175202 0.0 6 0.0 0.0 0.0 0.0748168954926102 0.0 7 0.0 0.0 0.0 0.07547318404956291 0.0 8 0.0 6.56288556952721E-4 0.0 0.07612947260651563 0.0 9 0.0 6.56288556952721E-4 0.0 0.08203606961909012 0.0 10 0.0 6.56288556952721E-4 0.0 0.08597380096080645 0.0 11 0.0 6.56288556952721E-4 0.0 0.08859895518861734 0.0 12 0.0 6.56288556952721E-4 0.0 0.08859895518861734 0.0 13 0.0 6.56288556952721E-4 0.0 0.08859895518861734 0.0 14 0.0 6.56288556952721E-4 0.0 0.08859895518861734 0.0 15 0.0 6.56288556952721E-4 0.0 0.08925524374557005 0.0 16 0.0 6.56288556952721E-4 0.0 0.09253668653033366 0.0 17 0.0 6.56288556952721E-4 0.0 0.0938492636442391 0.0 18 0.0 6.56288556952721E-4 0.0 0.09844328354290814 0.0 19 0.0 6.56288556952721E-4 0.0 0.10303730344157719 0.0 20 0.0 6.56288556952721E-4 0.0 0.1063187462263408 0.0 21 0.0 6.56288556952721E-4 0.0 0.11091276612500985 0.0 22 0.0 6.56288556952721E-4 0.0 0.11419420890977344 0.0 23 0.0 6.56288556952721E-4 0.0 0.11681936313758433 0.0 24 0.0 6.56288556952721E-4 0.0 0.1220696715932061 0.0 25 0.0 6.56288556952721E-4 0.0 0.1312577113905442 0.0 26 0.0 6.56288556952721E-4 0.0 0.14175832830178772 0.0 27 0.0 6.56288556952721E-4 0.0 0.21788780090830337 0.0 28 0.0 6.56288556952721E-4 0.0 0.3038616018691098 0.0 29 0.0 6.56288556952721E-4 0.0 0.39574199984249075 0.0 30 0.0 6.56288556952721E-4 0.0 0.5381566167012312 0.0 31 0.0 6.56288556952721E-4 0.0 0.6759772136613026 0.0 32 0.0 6.56288556952721E-4 0.0 0.813797810621374 0.0 33 0.0 6.56288556952721E-4 0.0 1.0028089150237576 0.0 34 0.0 6.56288556952721E-4 0.0 1.2856692830703804 0.0 35 0.0 6.56288556952721E-4 0.0 1.6335022182553225 0.0 36 0.0 6.56288556952721E-4 0.0 2.1152180190586196 0.0 37 0.0 6.56288556952721E-4 0.0 2.846323471503951 0.0 38 0.0 6.56288556952721E-4 0.0 3.58661696374662 0.0 39 0.0 6.56288556952721E-4 0.0 4.235686346572861 0.0 40 0.0 6.56288556952721E-4 0.0 5.032420654713464 0.0 41 0.0 6.56288556952721E-4 0.0 5.879032893182474 0.0 42 0.0 6.56288556952721E-4 0.0 6.719082246081958 0.0 43 0.0 6.56288556952721E-4 0.0 7.561100464652299 0.0 44 0.0 6.56288556952721E-4 0.0 8.390649200640537 0.0 45 0.0 6.56288556952721E-4 0.0 9.211009896831438 0.0 46 0.0 6.56288556952721E-4 0.0 10.056965846743497 0.0 47 0.0 6.56288556952721E-4 0.0 10.883233139946972 0.0 48 0.0 6.56288556952721E-4 0.0 11.622214055075736 0.0 49 0.0 6.56288556952721E-4 0.0 12.368414144330979 0.0 50 0.0 6.56288556952721E-4 0.0 13.032578163967132 0.0 51 0.0 6.56288556952721E-4 0.0 13.751214133830363 0.0 52 0.0 6.56288556952721E-4 0.0 14.511196282781613 0.0 53 0.0 6.56288556952721E-4 0.0 15.253458640695142 0.0 54 0.0 6.56288556952721E-4 0.0 15.96225028220408 0.0 55 0.001312577113905442 6.56288556952721E-4 0.0 16.590974719764787 0.0 56 0.001312577113905442 6.56288556952721E-4 0.0 17.239387814034075 0.0 57 0.001312577113905442 6.56288556952721E-4 0.0 17.86811225159478 0.0 58 0.001312577113905442 6.56288556952721E-4 0.0 18.433176699131074 0.0 59 0.001312577113905442 6.56288556952721E-4 0.0 19.051400519780536 0.0 60 0.001312577113905442 6.56288556952721E-4 0.0 19.63615362402541 0.0 61 0.001312577113905442 6.56288556952721E-4 0.0 20.186123434751792 0.0 62 0.001312577113905442 6.56288556952721E-4 0.0 20.825348489223742 0.0 63 0.001312577113905442 6.56288556952721E-4 0.0 21.447510041214922 0.0 64 0.001312577113905442 6.56288556952721E-4 0.0 22.04801407082666 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATCT 15 0.002221192 70.0 33 TGTCATC 25 2.384437E-4 55.999996 54 GCCATAG 25 2.384437E-4 55.999996 1 CTTTATA 50 9.731593E-10 55.999996 2 AGATAAT 20 0.006940526 52.5 32 TTGAACG 20 0.006940526 52.5 4 AGTTATC 20 0.006940526 52.5 32 TATGTCA 20 0.006940526 52.5 52 TTAATAC 35 2.0788782E-5 50.0 3 GTCTGAT 30 5.863271E-4 46.666664 25 GTAGCAG 30 5.863271E-4 46.666664 55 TCTTTAT 80 2.910383E-11 43.75 1 CTTAATA 50 3.4609766E-6 42.0 2 ATCGTAT 35 0.0012523439 40.0 39 TATCTTC 35 0.0012523439 40.0 66 TTCTTGT 35 0.0012523439 40.0 12 CCATAGA 35 0.0012523439 40.0 2 CCCCAGT 35 0.0012523439 40.0 17 TCTTTCA 35 0.0012523439 40.0 10 ATAACAT 35 0.0012523439 40.0 31 >>END_MODULE