FastQCFastQC Report
Wed 25 May 2016
SRR1780319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545853
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC554.9809078E-627.9808771
CTCTAAT508.372016E-526.381971
CCGTAAT551.599451E-423.9836081
GCTACTG500.00256829722.01522813
CGTAATA602.883273E-421.9849742
TTGGTAA602.8850604E-421.9829633
TGCACAG602.8868485E-421.9809475
ATACTGG755.5021686E-520.5155496
TAAACAG654.910521E-420.3180237
AAACAGG654.910521E-420.3180238
AATACTG654.9562694E-420.2901045
CACTAGC550.004498264319.9863411
CTATATC1101.2740384E-719.982684
GCGAAAA600.00741271918.33088923
GCCGATT600.00741271918.33088923
ATTTAGA600.007436269418.3208122
GTAATAC600.007444132618.3174553
CCATATG600.007444132618.3174558
CGACGAA2100.017.8071522
TATTAAG750.001277364817.6251215
Produced by FastQC (version 0.11.5)