FastQCFastQC Report
Wed 25 May 2016
SRR1780319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545853
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT194733.5674439821710244No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC62751.1495769007406755No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG47090.8626864741972655No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG39270.7194244604316548Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC10980.20115305769135647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1600.037.126162
TCTTCTG5350.036.18804644
CGTCTTC5650.034.26341642
ATGCCGT26650.032.4437944
GTCTTCT6050.032.00143
CCGTCTT6200.031.2239241
GACTATA454.006557E-529.3961871
TACTGTT454.0646388E-529.3342482
GCCGTCT6800.028.79237740
CCCATAG406.939773E-427.5589241
ACTGTTT407.0258765E-427.5008583
TATGCCG31600.027.29199243
TCTTTAT2200.027.0578561
TTTATAC2400.026.5841643
TGCCGTC7800.025.9471539
TTAATAC450.001396978624.4452083
GCGACAG551.592709E-423.99855235
CAGAGTT752.061901E-623.46524834
AATCTCG38700.021.65760836
GTCACAA39100.021.60483431
Produced by FastQC (version 0.11.5)