FastQCFastQC Report
Wed 25 May 2016
SRR1780318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102572
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGTG150.002219114770.0000159
TGTACTA150.002219114770.0000137
AATCGGT150.002219114770.0000158
ACGTATC200.006934085452.557
TCTGGAG200.006934085452.53
TGCGTTC200.006934085452.534
GATCTCT305.854251E-446.66666841
TGGACAA404.5654517E-543.7551
CTGGAGG404.5654517E-543.754
TTGGACA350.001250429240.050
CGAATGT350.001250429240.029
TTATCCA459.143404E-538.8888852
GAATGTG400.002409207435.030
CTTCCGA400.002409207435.018
TTCCGAC400.002409207435.019
GTATCAT400.002409207435.059
ATGTGTG450.004290363331.11110932
GCTTCCG450.004290363331.11110917
AAGGAGT450.004290363331.1111094
AATGTGT450.004290363331.11110931