FastQCFastQC Report
Wed 25 May 2016
SRR1780318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102572
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT87208.501345396404476No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG23542.2949732870568966No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC23152.2569512147564637No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG21012.0483172795694733RNA PCR Primer, Index 47 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5680.5537573606832275No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT2860.27882853020317433No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2580.2515306321413251Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT1880.183285886986702No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1670.1628124634403151No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1290.12576531607066255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG150.002219114770.0000129
TAAAGTC150.002219114770.0000145
AAGGTTC150.002219114770.0000124
GCCGCTA150.002219114770.0000114
AACGATT150.002219114770.0000139
GACGATT150.002219114770.0000132
CAGCTTA252.8225732E-670.01
ATTGGAG207.894287E-570.048
TTGTTCA252.3807473E-455.99999633
TTGGGAC252.3807473E-455.99999628
CTTTATA700.055.02
TTAGGTT200.006934085452.560
CGTATCA200.006934085452.548
TTTGTTC200.006934085452.532
GTCCTAC200.006934085452.549
TACGGGC200.006934085452.537
CCGCTAT200.006934085452.515
GTATCAT200.006934085452.549
TGTACGT200.006934085452.568
CGTTTGT200.006934085452.530
Produced by FastQC (version 0.11.5)