##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780318_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 102572 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.75020473423547 34.0 34.0 34.0 34.0 34.0 2 33.734167219124124 34.0 34.0 34.0 34.0 34.0 3 33.744754903872405 34.0 34.0 34.0 34.0 34.0 4 33.7614943649339 34.0 34.0 34.0 34.0 34.0 5 33.75180361112194 34.0 34.0 34.0 34.0 34.0 6 37.50688297001131 38.0 38.0 38.0 37.0 38.0 7 37.49533010958156 38.0 38.0 38.0 37.0 38.0 8 37.413114690168854 38.0 38.0 38.0 37.0 38.0 9 37.44001286900909 38.0 38.0 38.0 37.0 38.0 10-11 37.38175135514565 38.0 38.0 38.0 37.0 38.0 12-13 37.35479955543423 38.0 38.0 38.0 37.0 38.0 14-15 37.28020804898023 38.0 38.0 38.0 36.0 38.0 16-17 37.31441329017666 38.0 38.0 38.0 36.0 38.0 18-19 37.25918379284795 38.0 38.0 38.0 36.0 38.0 20-21 37.306253168506025 38.0 38.0 38.0 36.0 38.0 22-23 37.29732285614007 38.0 38.0 38.0 36.0 38.0 24-25 37.2900352922825 38.0 38.0 38.0 36.0 38.0 26-27 37.180926568654215 38.0 38.0 38.0 36.0 38.0 28-29 37.16794544320088 38.0 38.0 38.0 36.0 38.0 30-31 37.149241508403854 38.0 38.0 38.0 36.0 38.0 32-33 37.0252944273291 38.0 38.0 38.0 36.0 38.0 34-35 37.07998284132122 38.0 38.0 38.0 36.0 38.0 36-37 37.076092890847406 38.0 38.0 38.0 36.0 38.0 38-39 36.21063253129509 38.0 37.0 38.0 34.0 38.0 40-41 36.64243653238701 38.0 37.0 38.0 34.5 38.0 42-43 37.00246656007488 38.0 38.0 38.0 36.0 38.0 44-45 37.097580236321804 38.0 38.0 38.0 36.0 38.0 46-47 37.04475392894747 38.0 38.0 38.0 36.0 38.0 48-49 37.07058456498849 38.0 38.0 38.0 36.0 38.0 50-51 36.91051651522832 38.0 38.0 38.0 35.0 38.0 52-53 36.89626798736497 38.0 38.0 38.0 35.0 38.0 54-55 36.87941153531178 38.0 37.5 38.0 35.0 38.0 56-57 36.933398003353744 38.0 38.0 38.0 35.5 38.0 58-59 36.79876087041298 38.0 37.5 38.0 35.0 38.0 60-61 36.78282084779472 38.0 37.0 38.0 35.0 38.0 62-63 36.76704656241469 38.0 37.0 38.0 35.0 38.0 64-65 36.66493779978941 38.0 37.5 38.0 34.5 38.0 66-67 36.29880961665951 38.0 37.0 38.0 34.0 38.0 68-69 36.167072885387824 38.0 37.0 38.0 34.0 38.0 70-71 35.280749132316814 38.0 36.5 38.0 27.5 38.0 72-73 33.7782971961159 38.0 35.0 38.0 20.5 38.0 74-75 33.3680634091175 38.0 34.0 38.0 15.0 38.0 76 32.74223959755099 38.0 32.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03545443816773286 1101 2 -0.05038684204376409 1101 3 0.027699537750535796 1101 4 0.13531449557848418 1101 5 0.0026617452014008336 1101 6 -0.20323860002127958 1101 7 0.13860929610351036 1101 8 0.2179727973530916 1101 9 0.11697482120659686 1101 10-11 0.20200264880667618 1101 12-13 0.1844697703073308 1101 14-15 0.18562989171493882 1101 16-17 0.20788218153369087 1101 18-19 0.2846903301141239 1101 20-21 0.09889285008750903 1101 22-23 0.1094655757789127 1101 24-25 0.2435152483480536 1101 26-27 0.26780736309256525 1101 28-29 0.09324967667735962 1101 30-31 0.28102653366039476 1101 32-33 0.09933754098417324 1101 34-35 0.3507419334546853 1101 36-37 0.3932350382661909 1101 38-39 0.40499992004505003 1101 40-41 0.32798053057555876 1101 42-43 0.25139173311307417 1101 44-45 0.3385623721466686 1101 46-47 0.202964862853527 1101 48-49 0.12620539856258972 1101 50-51 0.4044187949974116 1101 52-53 0.2641209982028201 1101 54-55 0.35087892046005464 1101 56-57 0.2631350493983504 1101 58-59 0.5708072765748256 1101 60-61 0.5026916140419146 1101 62-63 0.44507548156657606 1101 64-65 0.3497948493765577 1101 66-67 0.3223665500126458 1101 68-69 0.23048406653460063 1101 70-71 0.4552628086515824 1101 72-73 0.882030245682877 1101 74-75 0.8011831972012544 1101 76 -0.34957060247857186 1102 1 0.06208106726251117 1102 2 0.06657222228371751 1102 3 0.02373128378228273 1102 4 0.031095615043817304 1102 5 -0.1431193742639323 1102 6 0.09918412345408001 1102 7 0.06342132617869822 1102 8 -0.05771642570454816 1102 9 0.07624800416398614 1102 10-11 -0.22197395938045617 1102 12-13 0.021353646664891812 1102 14-15 0.20881284910841913 1102 16-17 -1.3786859162223664E-4 1102 18-19 0.04053828333049836 1102 20-21 -0.026524860856525834 1102 22-23 0.04012555907047499 1102 24-25 -0.11665601313899998 1102 26-27 0.025743871029071386 1102 28-29 -0.02527580369022786 1102 30-31 -0.10723578881245288 1102 32-33 0.06947120848626298 1102 34-35 0.12110534197599065 1102 36-37 0.32076218947754853 1102 38-39 0.15917702948532053 1102 40-41 0.20506908529569046 1102 42-43 0.04305839977974557 1102 44-45 0.11435602294032066 1102 46-47 0.24034999234391563 1102 48-49 0.09038668511229275 1102 50-51 0.14115647252455688 1102 52-53 0.21316026303156121 1102 54-55 0.1519440833673258 1102 56-57 0.03234975282357766 1102 58-59 0.017340275739400113 1102 60-61 0.2706531846350657 1102 62-63 0.1547663754679931 1102 64-65 0.05415992874163322 1102 66-67 0.2773581957937665 1102 68-69 0.4270170656991752 1102 70-71 -0.18435707438935367 1102 72-73 -0.38091879358195513 1102 74-75 -0.2922796265247243 1102 76 0.30310274756320155 1103 1 -0.1998050606967965 1103 2 0.03136804082635791 1103 3 0.08931811330963058 1103 4 0.14108247811226704 1103 5 0.02092456457945957 1103 6 -0.023059217239662644 1103 7 -0.21056754935586497 1103 8 -0.11040390648829401 1103 9 -0.1637385793952788 1103 10-11 -0.21082853075312613 1103 12-13 -0.03701485824049655 1103 14-15 0.11575026700259627 1103 16-17 -0.016670836699624658 1103 18-19 -0.05972166020864478 1103 20-21 0.007379602905118077 1103 22-23 0.03616351641737481 1103 24-25 -0.24339246393140712 1103 26-27 -0.05139367543932849 1103 28-29 0.08250293684140075 1103 30-31 -0.04285084876717349 1103 32-33 0.20580181194659275 1103 34-35 -0.20128054840051135 1103 36-37 -0.26229396391708804 1103 38-39 -0.041126237977017865 1103 40-41 0.06462270913223733 1103 42-43 0.02739191013556308 1103 44-45 0.046512156769317414 1103 46-47 -0.08300020471005354 1103 48-49 0.08983465447808925 1103 50-51 -0.014187743032920253 1103 52-53 -0.1493002562964989 1103 54-55 -0.26717294712712913 1103 56-57 -0.19268308950528734 1103 58-59 0.05758959792964191 1103 60-61 0.1914123315730336 1103 62-63 0.13127362873126458 1103 64-65 0.1257212670316008 1103 66-67 -0.30403487796871076 1103 68-69 0.2633364887922198 1103 70-71 -0.20919845292863926 1103 72-73 -0.2584518455427087 1103 74-75 0.11752974821323647 1103 76 0.05122304831508018 1104 1 -0.0644226921359845 1104 2 0.003978237321312861 1104 3 0.016588426639430054 1104 4 0.07658433684832744 1104 5 -0.11717952463987302 1104 6 -0.05720685398953407 1104 7 -0.16853356103934658 1104 8 -0.28440815502786165 1104 9 -0.010406131174356403 1104 10-11 0.02858995039398593 1104 12-13 -0.02187943604187126 1104 14-15 0.0350346536196966 1104 16-17 -0.07664162799012075 1104 18-19 0.015444298368095133 1104 20-21 0.10167062786528192 1104 22-23 -0.009780455967117518 1104 24-25 0.07168985152265606 1104 26-27 -0.02068470039950654 1104 28-29 -0.2569487360210516 1104 30-31 -0.13881473618087625 1104 32-33 -0.08419420504757369 1104 34-35 0.121575266788021 1104 36-37 -0.08196337443222035 1104 38-39 0.09686499941013693 1104 40-41 -0.034122644027618776 1104 42-43 0.08591554263689005 1104 44-45 -0.2124296913453918 1104 46-47 0.059710894599554365 1104 48-49 0.015292700149892369 1104 50-51 -0.4261367605581512 1104 52-53 -0.205918684336865 1104 54-55 -0.039597270016138 1104 56-57 -0.5503570140937128 1104 58-59 -0.23256573929818813 1104 60-61 -0.4578242589739574 1104 62-63 -0.04579753430644473 1104 64-65 -0.42441149982979454 1104 66-67 -0.6863636087175138 1104 68-69 -0.744317520766991 1104 70-71 -0.6385863976976225 1104 72-73 -0.02828721463458095 1104 74-75 -0.522824739306678 1104 76 -0.6166340945420643 1105 1 -0.1942678469811412 1105 2 -0.0854073770642998 1105 3 0.024730284781284695 1105 4 0.11819272845671946 1105 5 0.10694634948600168 1105 6 0.1461897494070712 1105 7 0.03421369170790456 1105 8 -0.004637925257625852 1105 9 0.30220625643803345 1105 10-11 -0.0011553043512648742 1105 12-13 -0.08356774773018572 1105 14-15 0.1101220662071114 1105 16-17 -0.088654615003108 1105 18-19 -0.07822453280073915 1105 20-21 0.06835394454859767 1105 22-23 -0.10534738903405128 1105 24-25 -0.01332513349233011 1105 26-27 -0.03369668841148865 1105 28-29 0.13422259265028202 1105 30-31 0.01443201706587871 1105 32-33 0.11604305768969425 1105 34-35 -0.09152413381138302 1105 36-37 -0.13534749031633453 1105 38-39 -0.49365697861184543 1105 40-41 -0.15868558109055186 1105 42-43 -0.3203282136068637 1105 44-45 -0.172357263772966 1105 46-47 -0.2842701243814645 1105 48-49 -0.16948502212783012 1105 50-51 -0.09981308423446933 1105 52-53 -0.048538564456748645 1105 54-55 -0.5355263409452107 1105 56-57 -0.03836900210570349 1105 58-59 -0.07643437066681713 1105 60-61 0.06573967708997941 1105 62-63 -0.0298384933473983 1105 64-65 -0.11891699433528657 1105 66-67 -0.40142354877745845 1105 68-69 -0.09757676152622707 1105 70-71 -0.27718529879652465 1105 72-73 -0.7213941077414745 1105 74-75 -0.2995355285174739 1105 76 -1.1026231055310731 1106 1 -0.014902436272450359 1106 2 -0.13733306331834427 1106 3 -0.08287852432005849 1106 4 0.017629112967732397 1106 5 0.05371599774818492 1106 6 -0.028635425418102045 1106 7 -5.165034700524984E-4 1106 8 -0.06436551110461863 1106 9 -0.24478779428736175 1106 10-11 -0.13180450589599246 1106 12-13 -0.04879841258771478 1106 14-15 -0.02261460011165184 1106 16-17 0.023624896530130002 1106 18-19 -0.0532923754058956 1106 20-21 -0.0175191375931405 1106 22-23 -0.04461521076456165 1106 24-25 -0.15917368792297282 1106 26-27 -0.5768680692694375 1106 28-29 -0.3810159002001612 1106 30-31 -0.17671452296125523 1106 32-33 -0.48917821357635205 1106 34-35 -0.4609140722311693 1106 36-37 -0.1639196644109049 1106 38-39 -0.28082689824444884 1106 40-41 -0.1544584649231382 1106 42-43 -0.3735194253801666 1106 44-45 -0.09086785765669703 1106 46-47 -0.2490311096648341 1106 48-49 -0.5101070866304909 1106 50-51 -0.3558275921146503 1106 52-53 -0.2880881939317419 1106 54-55 0.03940059778770433 1106 56-57 0.013608870767690462 1106 58-59 -0.1255028395114124 1106 60-61 -0.2749084807223596 1106 62-63 -0.3000086217264908 1106 64-65 0.05229425709983815 1106 66-67 -0.20496702023137203 1106 68-69 0.1159969781669119 1106 70-71 0.052751555394053185 1106 72-73 0.4547618258771422 1106 74-75 0.4464982884118669 1106 76 1.6146558841359777 1107 1 -0.1291596699596056 1107 2 -0.12496709532437933 1107 3 -0.03274112467568102 1107 4 -0.09471530214084112 1107 5 -0.08486905532578959 1107 6 0.16811547710897656 1107 7 -0.11074604169948543 1107 8 0.24444692491025677 1107 9 0.05925606583441123 1107 10-11 0.07282667035272539 1107 12-13 0.1661040606058819 1107 14-15 0.2336357366521895 1107 16-17 0.1606069537530459 1107 18-19 0.009648785216988642 1107 20-21 0.04666804921649259 1107 22-23 0.26247972969559186 1107 24-25 0.1950008365664928 1107 26-27 0.24144784214820447 1107 28-29 0.19363790657822477 1107 30-31 0.04561022513939861 1107 32-33 0.1806652585934856 1107 34-35 0.12418614868590083 1107 36-37 0.2434298695079491 1107 38-39 0.10117778950812095 1107 40-41 0.3505795220373642 1107 42-43 0.3091749547049645 1107 44-45 0.1567747955035017 1107 46-47 -0.01812561906728405 1107 48-49 -0.06801699556955043 1107 50-51 -0.05579122162055228 1107 52-53 -0.015504811206383806 1107 54-55 0.24801232255735783 1107 56-57 -0.0457154462752456 1107 58-59 0.0270848537910382 1107 60-61 -0.29670383607555806 1107 62-63 0.050656823610012225 1107 64-65 0.347481228380623 1107 66-67 -0.0636070331631089 1107 68-69 0.26476576958886255 1107 70-71 0.09722458734621142 1107 72-73 -0.36902986548553685 1107 74-75 -0.11463108278270084 1107 76 -0.4144809228040529 1108 1 0.013564320851024547 1108 2 0.16326395160702845 1108 3 0.11801699806800059 1108 4 0.015675245939235083 1108 5 0.05100229354194852 1108 6 -0.08499906178173688 1108 7 0.13938851688272536 1108 8 0.08948794886824629 1108 9 -0.07891262468084648 1108 10-11 -0.02926719246315912 1108 12-13 -0.06447683863927978 1108 14-15 -0.08610171001666345 1108 16-17 -0.185018251366742 1108 18-19 0.08632092174472206 1108 20-21 0.05660569280034622 1108 22-23 -0.018773962460628013 1108 24-25 -0.07402443419163518 1108 26-27 -0.11152885624365894 1108 28-29 -0.058085099657411376 1108 30-31 0.06352292615679289 1108 32-33 -0.20136952972289635 1108 34-35 -0.3631325254197719 1108 36-37 -0.0866062315750824 1108 38-39 -0.21953110448597357 1108 40-41 -0.33980446220943605 1108 42-43 -0.12473380801245781 1108 44-45 0.19890147748577647 1108 46-47 0.13510050498916115 1108 48-49 0.05785763521483034 1108 50-51 0.07983726541587544 1108 52-53 -0.07413297005115282 1108 54-55 -0.059792075210943096 1108 56-57 0.13743519369849366 1108 58-59 0.05363555940310505 1108 60-61 0.29448093583123125 1108 62-63 -0.11962870313760732 1108 64-65 0.3477962923779998 1108 66-67 0.1948701575162488 1108 68-69 0.2903253363758722 1108 70-71 0.2863811347699112 1108 72-73 0.16028421393684766 1108 74-75 0.09759733861539388 1108 76 1.011223048315081 1109 1 0.0017660461330422095 1109 2 -0.048706851312680044 1109 3 0.09568123164464026 1109 4 -0.03618938577940867 1109 5 0.10873288411925586 1109 6 -0.3862996589947443 1109 7 -0.21592667887042438 1109 8 0.07320394090540816 1109 9 -0.08123511970407549 1109 10-11 -0.1483912800543976 1109 12-13 -0.05785440997305358 1109 14-15 0.18658574851021115 1109 16-17 0.20049601538840278 1109 18-19 -0.04554370997392709 1109 20-21 0.14009607103525212 1109 22-23 -0.1782762849556505 1109 24-25 -0.14796604003104363 1109 26-27 -0.0162269318906354 1109 28-29 0.11941987711762891 1109 30-31 -0.26957594577419997 1109 32-33 -0.15269003403609815 1109 34-35 -0.27095601579800643 1109 36-37 0.11791931587017501 1109 38-39 -0.10061935929685006 1109 40-41 0.07399779392858363 1109 42-43 -0.09406360230575928 1109 44-45 -0.05900164129325702 1109 46-47 0.10671298010528574 1109 48-49 0.045754118293793056 1109 50-51 -0.06419724022446616 1109 52-53 -0.032600123335825515 1109 54-55 -0.04384648795148394 1109 56-57 0.1907795865319386 1109 58-59 0.16468400001359385 1109 60-61 0.015754990243976863 1109 62-63 -0.23581317559946058 1109 64-65 -0.14839481578391656 1109 66-67 -0.21918425522429175 1109 68-69 -0.023397291361362704 1109 70-71 0.14841829469027346 1109 72-73 -0.655190238617898 1109 74-75 -0.5350901199114801 1109 76 -0.18965286409368076 1110 1 -0.07043438506939026 1110 2 0.001271069044747719 1110 3 -0.03244563538395795 1110 4 -0.083024204076942 1110 5 0.03353221913236837 1110 6 -0.1549699449910591 1110 7 -0.1875218681059465 1110 8 0.1491708993697003 1110 9 -0.17920379258767838 1110 10-11 -0.09211416755212554 1110 12-13 -0.06941375225946445 1110 14-15 -0.204600577697029 1110 16-17 0.13247123423662543 1110 18-19 -0.03365966706149237 1110 20-21 0.09127408490645905 1110 22-23 -0.17278884437586584 1110 24-25 0.10946311029236 1110 26-27 0.06222073935646932 1110 28-29 0.1898929009896264 1110 30-31 0.12976686015873184 1110 32-33 0.0832244528070305 1110 34-35 0.008617717220154475 1110 36-37 -0.11237135613023952 1110 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-0.030181928979231998 2115 62-63 -0.026780665566327855 2115 64-65 -0.03532346609231496 2115 66-67 -0.09776318699901054 2115 68-69 -0.27181488502634465 2115 70-71 -0.9542361077661496 2115 72-73 -1.6266530554358525 2115 74-75 -1.2877364419951363 2115 76 -1.3915813797291996 2116 1 0.00732100534300173 2116 2 0.03370512807761372 2116 3 -0.13698300193200197 2116 4 -0.005033310210500019 2116 5 0.11884988712483135 2116 6 0.17357045693483997 2116 7 0.23104627089342245 2116 8 0.06968848362760127 2116 9 -0.045490164787800325 2116 10-11 0.12543868988978346 2116 12-13 0.05480123622703559 2116 14-15 0.105622888913814 2116 16-17 -0.07879498933466067 2116 18-19 0.01870060088909753 2116 20-21 -0.0072579435632889044 2116 22-23 0.17567122470514818 2116 24-25 0.10325497757308 2116 26-27 0.17831740044083944 2116 28-29 0.11988949927306436 2116 30-31 0.30687854113004676 2116 32-33 0.17552218150705556 2116 34-35 0.3805038382165904 2116 36-37 0.2667761213660924 2116 38-39 0.06445953722526099 2116 40-41 0.1864655912664972 2116 42-43 0.15912982835117617 2116 44-45 0.0867824935285526 2116 46-47 0.27725291140627917 2116 48-49 0.2878242127549342 2116 50-51 0.3724976932233659 2116 52-53 0.23888173583119965 2116 54-55 -0.03366239606656052 2116 56-57 0.28761567498191454 2116 58-59 0.14290694977744067 2116 60-61 0.13046355615209393 2116 62-63 0.3178579278784355 2116 64-65 0.23246875227104624 2116 66-67 0.42702924842534173 2116 68-69 0.20253121872880797 2116 70-71 0.49444931658808855 2116 72-73 0.11131362570154835 2116 74-75 0.11940215144961996 2116 76 1.0696054012562541 2117 1 0.08025385772923954 2117 2 0.06590546104099104 2117 3 0.09839435655856477 2117 4 -0.10926471975544416 2117 5 -0.0011417887908180546 2117 6 0.08268532929887584 2117 7 0.20895970041617318 2117 8 -0.29397688818149703 2117 9 0.10084723809788443 2117 10-11 0.20695922263117694 2117 12-13 0.06060034832469796 2117 14-15 0.17111955928007205 2117 16-17 0.029355676592089708 2117 18-19 0.022890390012300088 2117 20-21 0.07835526398388026 2117 22-23 0.12259825023234328 2117 24-25 -0.1744017926821968 2117 26-27 0.17197028612341114 2117 28-29 -0.3397317548889802 2117 30-31 0.02317987298354751 2117 32-33 0.07554299171793133 2117 34-35 0.316352894820362 2117 36-37 -0.022832646669421308 2117 38-39 0.20053750614867738 2117 40-41 0.2783773559723812 2117 42-43 0.27162982835117333 2117 44-45 0.07567677765729997 2117 46-47 0.1679993043030592 2117 48-49 0.18931561120110985 2117 50-51 0.34529181087042105 2117 52-53 -0.09941598891907688 2117 54-55 0.3711170156981467 2117 56-57 -0.0034567445519329 2117 58-59 0.0640986811869908 2117 60-61 -0.025176010995522802 2117 62-63 0.35265260046444524 2117 64-65 0.034348607986920854 2117 66-67 0.34389245597250806 2117 68-69 0.2257970344890765 2117 70-71 0.19763327885223703 2117 72-73 -0.3959422011574958 2117 74-75 -0.01962393068134105 2117 76 -0.480852423383034 2118 1 -0.03507722771447419 2118 2 -0.2662417518150235 2118 3 -0.19924535934644894 2118 4 -0.15364380003380518 2118 5 -0.1626167869972832 2118 6 0.05311362401155861 2118 7 -0.051776363863901054 2118 8 -0.17492961075843994 2118 9 -0.1801915738685409 2118 10-11 0.015675773316310426 2118 12-13 0.1304834102373178 2118 14-15 -0.04771249466927685 2118 16-17 0.04148710639336883 2118 18-19 -0.239043751604747 2118 20-21 -0.11063246827811213 2118 22-23 -0.012856230002967095 2118 24-25 0.07000598405932124 2118 26-27 -0.11398996927511007 2118 28-29 -0.06897345084994555 2118 30-31 -0.21111240049374658 2118 32-33 0.002273747152322869 2118 34-35 -0.018735705509648426 2118 36-37 0.019781601124535086 2118 38-39 -0.09222381101898236 2118 40-41 -0.3166796782481498 2118 42-43 -0.009358764804723307 2118 44-45 -0.02727895804827085 2118 46-47 0.04826331176413845 2118 48-49 0.04342279249101466 2118 50-51 -0.07828233361626502 2118 52-53 -0.03398087879639888 2118 54-55 -0.03344572194443174 2118 56-57 0.03247840144131686 2118 58-59 0.14917652034331041 2118 60-61 -0.02092176033191606 2118 62-63 -0.09877146151991667 2118 64-65 -0.07987048950931808 2118 66-67 0.13817468568770153 2118 68-69 0.25032533637586596 2118 70-71 0.36006243445881125 2118 72-73 0.2276606397923615 2118 74-75 0.5566225719372042 2118 76 -0.21493664750241237 2119 1 -0.13198555997975348 2119 2 -0.015163014685107612 2119 3 -0.07284442515297229 2119 4 0.07302628441387071 2119 5 -0.1005128579732073 2119 6 -0.11290508833945267 2119 7 -0.14095196660382214 2119 8 0.015725606341860043 2119 9 -0.030563969590623685 2119 10-11 0.01148187120725197 2119 12-13 -0.15804851041319523 2119 14-15 -0.7350496160432272 2119 16-17 -0.09682621868032726 2119 18-19 0.0018742074103599293 2119 20-21 0.04541059576255435 2119 22-23 -0.11476306698905603 2119 24-25 -0.16795701846129418 2119 26-27 0.02855285979311617 2119 28-29 -0.21879330530943975 2119 30-31 -0.15989552804818175 2119 32-33 -0.42157381981482445 2119 34-35 -0.31799803444258856 2119 36-37 -0.3981418120994249 2119 38-39 -0.36876501664123396 2119 40-41 -0.2422218949639472 2119 42-43 -0.19690949749151798 2119 44-45 -0.23676757256529157 2119 46-47 -0.08671767682901788 2119 48-49 0.035269960717045024 2119 50-51 -0.0277418969947405 2119 52-53 0.030885756540612874 2119 54-55 -0.2041638831782535 2119 56-57 -0.2542093415415465 2119 58-59 -0.10397424652130383 2119 60-61 0.12793684321972876 2119 62-63 0.005178923692483295 2119 64-65 -0.008574153655366956 2119 66-67 0.30308261921185675 2119 68-69 0.11549387570170921 2119 70-71 0.38220680722479017 2119 72-73 0.18455387685819602 2119 74-75 -0.2782487628486834 2119 76 -1.742784442321625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 1.0 22 3.0 23 10.0 24 23.0 25 50.0 26 106.0 27 193.0 28 366.0 29 620.0 30 893.0 31 1431.0 32 2221.0 33 3786.0 34 6244.0 35 10467.0 36 14914.0 37 61242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.145068829700115 15.306321413251181 12.519985961080996 37.02862379596771 2 18.606442303942597 17.84892563272628 37.58335608158172 25.961275981749406 3 18.02343719533596 16.926646648208088 33.98003353741762 31.069882619038335 4 15.052840931248292 24.567133330733533 30.99871309909137 29.381312638926804 5 15.681667511601608 28.96696954334516 39.27875053620871 16.07261240884452 6 28.7173887610654 37.70132199820614 16.002417813828334 17.57887142690013 7 26.77143859922786 23.688725968100456 21.313808836719573 28.226026595952113 8 22.923409897437896 37.380571695979405 15.282923214912453 24.41309519167024 9 21.63065943922318 15.393089732090628 16.059938384744374 46.91631244394181 10-11 26.221093475802366 26.39560503841204 22.98239285575011 24.400908630035488 12-13 24.61539211480716 17.885485317630543 25.659049253207506 31.840073314354793 14-15 22.039640447685528 16.744823148617556 19.808524743594745 41.40701166010217 16-17 28.951370744452674 24.545197519790975 17.66368989587802 28.83974183987833 18-19 31.58756775728269 22.479331591467457 18.098018952540652 27.835081698709203 20-21 24.89032094528721 20.215555902195533 17.904496353780758 36.989626798736495 22-23 23.023827165308273 24.514487384471394 19.084642982490347 33.377042467729986 24-25 30.283118199898606 24.738232656085483 19.05052060991304 25.928128534102875 26-27 29.822953632570293 26.261065397964355 19.83338532932964 24.08259564013571 28-29 24.210310806067934 23.695062980150528 23.709199391646845 28.385426822134697 30-31 28.412724720196547 19.15483757750653 27.992044612564833 24.44039308973209 32-33 25.0248605857349 18.545509495768826 26.344908942011465 30.084720976484814 34-35 22.571461997426198 29.161954529501227 23.3148422571462 24.951741215926372 36-37 29.598720898490814 27.312521935810942 20.291112584331007 22.797644581367233 38-39 23.184689778887023 22.676266427485086 26.752427563077642 27.38661623055025 40-41 22.512966501579378 17.650040946847092 28.480482002885775 31.356510548687748 42-43 28.097823967554497 17.67051437039348 27.080977264750615 27.150684397301404 44-45 21.640408688530982 24.076746090551026 30.46591662441992 23.81692859649807 46-47 25.91301719767578 20.119038334048277 27.026381468626916 26.941562999649026 48-49 25.75946652107788 18.53624770892641 22.69966462582381 33.0046211441719 50-51 23.067211324727996 18.980813477362243 27.554303318644465 30.397671879265296 52-53 18.52893577194556 18.93742931794252 36.52702491908123 26.00660999103069 54-55 19.459014155909994 20.081991186678625 30.978239675544984 29.480754981866397 56-57 24.40578325468939 21.200230082283664 24.450142339039893 29.94384432398705 58-59 20.335471668681514 21.47905861248684 31.45497796669656 26.730491752135087 60-61 24.67340014818859 26.11726397067426 25.1140662169013 24.095269664235854 62-63 19.553581874195686 35.82507896891939 20.211168740007018 24.4101704168779 64-65 18.486526537456616 37.94651561829739 19.24014350894981 24.32681433529618 66-67 18.64933900089693 37.30745232617089 19.42001715867878 24.623191514253403 68-69 18.364660921109074 37.6140662169013 19.58721678430761 24.43405607768202 70-71 18.517724135241586 35.691026790937094 20.435888936551887 25.35536013726943 72-73 18.72830792029014 32.40114261201887 21.65113286276957 27.21941660492142 74-75 18.84822368677612 30.96995281363335 22.809831143001986 27.371992356588542 76 18.966189603400537 31.641188628475607 22.39987520960886 26.992746558514995 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 1.5 16 5.0 17 7.0 18 6.5 19 8.5 20 12.0 21 13.0 22 22.0 23 44.5 24 56.5 25 55.0 26 97.5 27 157.5 28 236.0 29 297.0 30 361.5 31 494.5 32 679.0 33 795.0 34 902.0 35 1139.0 36 1348.0 37 1427.0 38 1824.0 39 4481.5 40 7927.0 41 8491.5 42 7871.0 43 6915.0 44 5489.5 45 4665.5 46 4311.0 47 4152.0 48 3845.5 49 3422.0 50 3146.0 51 2977.5 52 2457.5 53 2099.0 54 2092.0 55 2040.0 56 1937.5 57 1905.5 58 1924.0 59 2036.0 60 2245.5 61 2330.5 62 2318.0 63 2447.5 64 2674.0 65 2844.5 66 2955.0 67 2992.0 68 2923.5 69 2721.5 70 2483.5 71 2379.0 72 2164.5 73 1708.5 74 1243.5 75 1020.0 76 833.5 77 474.5 78 200.5 79 99.0 80 59.5 81 15.5 82 6.5 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 102572.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.16437234332956 #Duplication Level Percentage of deduplicated Percentage of total 1 63.13948970340629 28.516554225324647 2 20.489573889392567 18.507974885933784 3 7.552993999050209 10.233786998401124 4 3.309156844968268 5.978239675544983 5 1.7420023313042352 3.933822095698631 6 1.0965764365583042 2.9715711890184457 7 0.6411086646807409 2.026868931092306 8 0.5007986875620601 1.8094606715282922 9 0.3583300954107845 1.4565378465858128 >10 1.1224798169494452 7.043832624887883 >50 0.023744765358545958 0.7653160706625589 >100 0.012951690195570523 1.1006902468509925 >500 0.002158615032595087 0.5537573606832275 >1k 0.006475845097785262 6.600241781382834 >5k 0.002158615032595087 8.501345396404476 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 8720 8.501345396404476 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 2354 2.2949732870568966 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 2315 2.2569512147564637 No Hit CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG 2101 2.0483172795694733 RNA PCR Primer, Index 47 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 568 0.5537573606832275 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 286 0.27882853020317433 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 258 0.2515306321413251 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 188 0.183285886986702 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 167 0.1628124634403151 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 129 0.12576531607066255 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00487462465390165 0.0 2 0.0 0.0 0.0 0.0077993994462426395 0.0 3 0.0 0.0 0.0 0.0077993994462426395 0.0 4 0.0 0.0 0.0 0.0389969972312132 0.0 5 0.0 0.0 0.0 0.04972117146979683 0.0 6 0.0 0.0 0.0 0.05069609640057716 0.0 7 0.0 0.0 0.0 0.05069609640057716 0.0 8 0.0 0.0 0.0 0.05362087119291815 0.0 9 0.0 0.0 0.0 0.07311936980852474 9.749249307803299E-4 10 0.0 0.0 0.0 0.07994384432398706 9.749249307803299E-4 11 0.0 0.0 0.0 0.08091876925476739 9.749249307803299E-4 12 0.0 0.0 0.0 0.08286861911632805 9.749249307803299E-4 13 0.0 0.0 0.0 0.08384354404710838 9.749249307803299E-4 14 0.0 0.0 0.0 0.08579339390866904 9.749249307803299E-4 15 0.0 0.0 0.0 0.08579339390866904 9.749249307803299E-4 16 0.0 0.0 0.0 0.08579339390866904 9.749249307803299E-4 17 0.0 0.0 0.0 0.08969309363179036 9.749249307803299E-4 18 0.0 0.0 0.0 0.094567718285692 9.749249307803299E-4 19 0.0 0.0 0.0 0.09846741800881333 9.749249307803299E-4 20 0.0 0.0 0.0 0.10821666731661662 9.749249307803299E-4 21 0.0 0.0 0.0 0.11211636703973794 9.749249307803299E-4 22 0.0 0.0 0.0 0.12186561634754124 9.749249307803299E-4 23 0.0 0.0 0.0 0.13258979058612486 9.749249307803299E-4 24 0.0 0.0 0.0 0.13648949030924618 9.749249307803299E-4 25 0.0 0.0 0.0 0.15013843934017082 9.749249307803299E-4 26 0.0 0.0 0.0 0.16963693795577742 9.749249307803299E-4 27 0.0 0.0 0.0 0.26030495651834806 9.749249307803299E-4 28 0.0 0.0 0.0 0.3880201224505713 9.749249307803299E-4 29 0.0 0.0 0.0 0.5245096127598174 9.749249307803299E-4 30 0.0 0.0 0.0 0.6551495534843818 9.749249307803299E-4 31 0.0 0.0 0.0 0.8043130678937722 9.749249307803299E-4 32 0.0 0.0 0.0 1.0148968529423235 9.749249307803299E-4 33 0.0 0.0 0.0 1.2576531607066257 9.749249307803299E-4 34 0.0 0.0 0.0 1.5676792886947706 9.749249307803299E-4 35 0.0 0.0 0.0 2.0356432554693287 9.749249307803299E-4 36 0.0 0.0 0.0 2.694692508676832 9.749249307803299E-4 37 0.0 0.0 0.0 3.449284405100807 9.749249307803299E-4 38 0.0 0.0 0.0 4.2438482236867765 9.749249307803299E-4 39 0.0 0.0 0.0 5.038412042272745 9.749249307803299E-4 40 0.0 0.0 0.0 5.877822407674609 9.749249307803299E-4 41 0.0 0.0 0.0 6.655812502437312 9.749249307803299E-4 42 0.0 0.0 0.0 7.53519479000117 9.749249307803299E-4 43 0.0 0.0 0.0 8.426276176734392 9.749249307803299E-4 44 0.0 0.0 0.0 9.233514019420504 9.749249307803299E-4 45 0.0 0.0 0.0 10.094372733299537 9.749249307803299E-4 46 0.0 0.0 0.0 10.935732948562961 9.749249307803299E-4 47 0.0 0.0 0.0 11.661077097063526 9.749249307803299E-4 48 0.0 0.0 0.0 12.429317942518425 9.749249307803299E-4 49 0.0 0.0 0.0 13.173185664703817 9.749249307803299E-4 50 0.0 0.0 0.0 13.821510743672738 9.749249307803299E-4 51 0.0 0.0 0.0 14.543930117380961 9.749249307803299E-4 52 0.0 0.0 0.0 15.293647389151035 9.749249307803299E-4 53 0.0 0.0 0.0 15.998518114105213 9.749249307803299E-4 54 0.0 0.0 0.0 16.658542292243496 9.749249307803299E-4 55 0.0 0.0 0.0 17.277619623289006 9.749249307803299E-4 56 0.0 0.0 0.0 17.900596654057637 9.749249307803299E-4 57 0.0 0.0 0.0 18.503100261279883 9.749249307803299E-4 58 0.0 0.0 0.0 19.129976991771635 9.749249307803299E-4 59 0.0 0.0 0.0 19.74515462309402 9.749249307803299E-4 60 0.0 0.0 0.0 20.317435557462076 9.749249307803299E-4 61 0.0 0.0 0.0 20.887766641968568 9.749249307803299E-4 62 0.0 0.0 0.0 21.472721600436767 9.749249307803299E-4 63 0.0 0.0 0.0 22.096673556136178 9.749249307803299E-4 64 0.0 0.0 0.0 22.706976562804662 9.749249307803299E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTG 15 0.0022191147 70.00001 29 TAAAGTC 15 0.0022191147 70.00001 45 AAGGTTC 15 0.0022191147 70.00001 24 GCCGCTA 15 0.0022191147 70.00001 14 AACGATT 15 0.0022191147 70.00001 39 GACGATT 15 0.0022191147 70.00001 32 CAGCTTA 25 2.8225732E-6 70.0 1 ATTGGAG 20 7.894287E-5 70.0 48 TTGTTCA 25 2.3807473E-4 55.999996 33 TTGGGAC 25 2.3807473E-4 55.999996 28 CTTTATA 70 0.0 55.0 2 TTAGGTT 20 0.0069340854 52.5 60 CGTATCA 20 0.0069340854 52.5 48 TTTGTTC 20 0.0069340854 52.5 32 GTCCTAC 20 0.0069340854 52.5 49 TACGGGC 20 0.0069340854 52.5 37 CCGCTAT 20 0.0069340854 52.5 15 GTATCAT 20 0.0069340854 52.5 49 TGTACGT 20 0.0069340854 52.5 68 CGTTTGT 20 0.0069340854 52.5 30 >>END_MODULE