FastQCFastQC Report
Wed 25 May 2016
SRR1780317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553515
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC302.54306E-643.955281
GTTAAGC301.3087211E-436.626093
GCTACAG554.9975297E-627.9690153
ATTACCA406.991128E-427.5242739
CAACGGT407.0170633E-427.50684213
TCAGATG609.824549E-625.6382644
TTAATAC450.001406298624.4173953
AATACCC450.001406298624.4173953
AAGTAGT551.5942926E-423.99511727
TAATACA752.0808402E-623.4406974
CAGTGTA500.002566855922.01742234
TGAATAT803.6018882E-622.00547213
CAAGTGC500.00258173221.99552326
ATCAGTA602.874001E-421.99552326
TTGGGTC500.002583088121.99353610
CTATACA500.002593956421.977641
GTATAAA500.002593956421.977641
TATGCAG755.4743265E-520.52915630
CGTCAGA755.5087236E-520.5124652
GATTTCC550.00445776920.0176537
Produced by FastQC (version 0.11.5)