SRR1780317_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780317_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553515
Sequences flagged as poor quality	0
Sequence length	50
%GC	60

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT	25202	4.5530834756059	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC	6982	1.2613930968447107	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG	6819	1.231944933741633	No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG	4478	0.8090114992366964	RNA PCR Primer, Index 14 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC	1443	0.26069754207203055	No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT	590	0.10659151061850175	RNA PCR Primer, Index 35 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTTAT	150	0.0	41.14498	1
CCTTATA	25	0.0023298133	35.267128	1
TACATTG	25	0.0023516228	35.20022	5
TCTTCTG	535	0.0	33.719833	44
GTCTTCT	535	0.0	33.719833	43
ATGCCGT	3480	0.0	33.442734	44
CTTTATA	190	0.0	32.430042	2
TTTATAC	225	0.0	31.297565	3
CCGTCTT	600	0.0	30.066853	41
CAATACC	30	0.005734582	29.341467	4
TGTCTAC	45	4.0679417E-5	29.330864	34
CGTCTTC	620	0.0	29.096954	42
TATGCCG	4230	0.0	27.513172	43
TTAATAC	65	5.9070953E-7	27.084433	2
GCCGTCT	700	0.0	25.771585	40
TCTATGG	60	9.7195825E-6	25.673784	2
TCTGTCC	45	0.0013950022	24.451221	3
ACAGTCT	45	0.0013979429	24.442387	36
CTATGGC	65	1.807205E-5	23.698877	3
CGTATGC	5245	0.0	22.105	41