FastQCFastQC Report
Wed 25 May 2016
SRR1780316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103308
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGCC150.00221916170.018
TAAGGGA150.00221916170.04
TACGTGG308.3264495E-658.3333325
TTGGCCT200.006934229852.521
GTAGAAA200.006934229852.51
TGCGAAC200.006934229852.55
GCCTAGG200.006934229852.51
TATAAGA352.0741818E-550.02
GTAAGGT305.854452E-446.66666419
ATAAGAG350.001250471740.03
CGGAGAT459.1438706E-538.88888521
GACAGGC400.002409289435.034
GTATAAG400.002409289435.01
TATACTC400.002409289435.03
GCTAAAT400.002409289435.028
TAAGAGA400.002409289435.04
CGCCTAA450.004290507631.11110925
ACAGGGC604.9533276E-429.1666663
ACCCCTA604.9533276E-429.1666667
TCTGCCG500.00718050428.00000211
Produced by FastQC (version 0.11.5)