##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780316_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 103308 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.409000271034188 34.0 27.0 34.0 12.0 34.0 2 29.37584698184071 34.0 27.0 34.0 12.0 34.0 3 29.15317303597011 34.0 27.0 34.0 11.0 34.0 4 29.004123591590197 34.0 27.0 34.0 11.0 34.0 5 28.97232547334185 34.0 27.0 34.0 11.0 34.0 6 31.85583885081504 37.0 31.0 38.0 11.0 38.0 7 32.232547334185156 37.0 31.0 38.0 12.0 38.0 8 32.16630851434545 37.0 31.0 38.0 11.0 38.0 9 32.46667247454214 38.0 31.0 38.0 12.0 38.0 10-11 32.16811863553646 37.0 31.0 38.0 11.0 38.0 12-13 32.26934990513803 37.0 31.0 38.0 11.0 38.0 14-15 32.147505517481704 37.0 31.0 38.0 11.0 38.0 16-17 32.189975606922985 38.0 31.0 38.0 11.0 38.0 18-19 32.234115460564524 38.0 31.0 38.0 11.0 38.0 20-21 32.21487687303984 38.0 31.0 38.0 11.0 38.0 22-23 32.22979343322879 38.0 31.0 38.0 11.0 38.0 24-25 32.20075889572928 38.0 31.0 38.0 11.0 38.0 26-27 32.15040945522128 38.0 31.0 38.0 11.0 38.0 28-29 32.21047740736439 38.0 31.0 38.0 11.0 38.0 30-31 32.227242807914195 38.0 31.0 38.0 11.0 38.0 32-33 32.30548941030705 38.0 31.0 38.0 11.0 38.0 34-35 32.24322898517056 38.0 31.0 38.0 11.0 38.0 36-37 32.17984086421187 38.0 31.0 38.0 11.0 38.0 38-39 32.16850098733883 38.0 31.0 38.0 11.0 38.0 40-41 32.25745828009447 38.0 31.0 38.0 11.0 38.0 42-43 32.31164575831494 38.0 31.0 38.0 11.0 38.0 44-45 32.44571572385488 38.0 31.0 38.0 11.0 38.0 46-47 32.32407945173655 38.0 31.0 38.0 11.0 38.0 48-49 32.24061544120494 38.0 31.0 38.0 11.0 38.0 50-51 32.19646590777094 38.0 31.0 38.0 11.0 38.0 52-53 32.27368161226623 38.0 31.0 38.0 11.0 38.0 54-55 32.249791884462 38.0 31.0 38.0 11.0 38.0 56-57 32.19711929376234 38.0 31.0 38.0 11.0 38.0 58-59 32.22173500600147 38.0 31.0 38.0 11.0 38.0 60-61 32.26860456111821 38.0 31.0 38.0 11.0 38.0 62-63 32.10568881403183 37.0 31.0 38.0 11.0 38.0 64-65 32.03290645448561 37.0 31.0 38.0 11.0 38.0 66-67 31.913457815464437 37.0 29.5 38.0 11.0 38.0 68-69 31.843952065667715 37.0 29.0 38.0 11.0 38.0 70-71 31.71821156154412 37.0 28.0 38.0 11.0 38.0 72-73 31.58963487822821 37.0 27.5 38.0 11.0 38.0 74-75 31.576683315909705 37.0 27.0 38.0 11.0 38.0 76 29.8588299066868 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2151032720049315 1101 2 0.28355556650245006 1101 3 0.292562243639658 1101 4 0.2065384670544752 1101 5 -0.12979638285291983 1101 6 -0.4071033533584263 1101 7 -0.6590331880533853 1101 8 -0.6141457657861658 1101 9 -0.7877380538419096 1101 10-11 -0.10114716627690967 1101 12-13 0.48635925776643063 1101 14-15 -0.4652097957842116 1101 16-17 -0.5930470389697291 1101 18-19 -0.717942674361435 1101 20-21 -0.18704080054374117 1101 22-23 -0.39665167352308117 1101 24-25 -0.8066332872160871 1101 26-27 -1.2970422406592732 1101 28-29 -0.6677180422636191 1101 30-31 -0.5773790093022235 1101 32-33 -0.811872564860515 1101 34-35 -0.6220082103063973 1101 36-37 -0.6618329336204631 1101 38-39 -0.0071405609026911065 1101 40-41 0.2155574530144193 1101 42-43 -0.35804054766088456 1101 44-45 -0.052249737975806454 1101 46-47 -0.13434509560220675 1101 48-49 0.05760618067137102 1101 50-51 -0.3739644750306823 1101 52-53 -0.3955963025444511 1101 54-55 -0.15685512199389962 1101 56-57 -0.1964032428150304 1101 58-59 -0.20098287822785466 1101 60-61 -0.04931152958662466 1101 62-63 -0.5040823952951214 1101 64-65 -0.22817918259990222 1101 66-67 -0.06935369196270358 1101 68-69 -0.11649995872739183 1101 70-71 0.07313323630585344 1101 72-73 -0.23646083641264326 1101 74-75 -0.832288210003238 1101 76 -1.3229386668143164 1102 1 0.22706340489529708 1102 2 0.2783506938557174 1102 3 0.24962204430411106 1102 4 0.1348330406978846 1102 5 0.5028171276675657 1102 6 -0.0886371296596451 1102 7 -0.008368736226138651 1102 8 -0.26971054983932063 1102 9 -0.4199916529559786 1102 10-11 -0.22306023382951423 1102 12-13 0.11673024336997173 1102 14-15 0.10440537586584497 1102 16-17 -0.002086352369509825 1102 18-19 -0.029736694294989263 1102 20-21 -0.10499484262568615 1102 22-23 0.39336770632188944 1102 24-25 -0.03485864712749276 1102 26-27 -0.1054309449782096 1102 28-29 -0.16618149741312038 1102 30-31 0.05336573045413928 1102 32-33 0.10653828785266484 1102 34-35 -0.12603644950905135 1102 36-37 -0.01402008755180617 1102 38-39 0.08575811019365176 1102 40-41 0.1653498118184018 1102 42-43 -0.2522404369189175 1102 44-45 0.021310616398501736 1102 46-47 0.05649100628041026 1102 48-49 -0.2642238304028268 1102 50-51 0.0499834762428506 1102 52-53 -0.025911917162389386 1102 54-55 -0.32678867681117296 1102 56-57 -0.23193757282610505 1102 58-59 -0.19230347623449262 1102 60-61 0.5106275623292191 1102 62-63 0.20971882286656296 1102 64-65 -0.14537187362980575 1102 66-67 -0.29703918476447555 1102 68-69 -0.17544237290236353 1102 70-71 0.24427111006000857 1102 72-73 0.22718811153863072 1102 74-75 0.21926217759366295 1102 76 0.20577118922112092 1103 1 -0.16122952960702364 1103 2 0.18170293728016063 1103 3 -0.6907724889604587 1103 4 -0.2812319412515727 1103 5 -0.004016291620725809 1103 6 -0.621373076023751 1103 7 -0.2724997315845741 1103 8 -0.12934180306262988 1103 9 -0.48392924965530426 1103 10-11 -0.7079042255778347 1103 12-13 -0.12291648174606706 1103 14-15 -0.18799405683142112 1103 16-17 -0.3018736138444922 1103 18-19 -0.3821606241557447 1103 20-21 -0.43835611132088914 1103 22-23 -0.3732839182268535 1103 24-25 -0.13509717214038375 1103 26-27 0.14878514579023516 1103 28-29 -0.39931068771740286 1103 30-31 -0.8780744448939757 1103 32-33 -0.387775791569716 1103 34-35 -0.4446091674048738 1103 36-37 -0.050598220610076794 1103 38-39 -0.7591555050719521 1103 40-41 -0.36462053372053305 1103 42-43 -0.5534201687394393 1103 44-45 -0.08468474203368004 1103 46-47 -0.31005102569483256 1103 48-49 -0.08115910631823908 1103 50-51 -0.4679252395900555 1103 52-53 -0.5420155788436709 1103 54-55 -0.5669382384408799 1103 56-57 -0.39767377705508977 1103 58-59 -0.40008454895239254 1103 60-61 0.12157392643442222 1103 62-63 -0.04044234268268809 1103 64-65 -0.335328036600405 1103 66-67 -0.15235120126782675 1103 68-69 -0.06408342499107178 1103 70-71 -0.262040132848707 1103 72-73 -0.22692483327828583 1103 74-75 0.09669900069835435 1103 76 -0.4066707911811491 1104 1 1.1420195678659013 1104 2 0.4777318181465873 1104 3 0.3958701103175173 1104 4 0.22779332950655018 1104 5 -0.18091159847993055 1104 6 0.11869912614231382 1104 7 0.13905261498582178 1104 8 -0.5950457741817665 1104 9 -1.7473701992543056E-4 1104 10-11 0.4996770018589842 1104 12-13 -0.27995619293739793 1104 14-15 0.6026196615801283 1104 16-17 0.5338664583576715 1104 18-19 0.551052372234146 1104 20-21 -0.24160520879381409 1104 22-23 -0.09169547057341987 1104 24-25 0.128440733996797 1104 26-27 0.2733338822523237 1104 28-29 -0.06898577797010574 1104 30-31 0.3692179738785839 1104 32-33 0.2530184322570008 1104 34-35 0.3441994968550546 1104 36-37 0.22918517289172513 1104 38-39 0.3058264443865326 1104 40-41 -0.09747251928009604 1104 42-43 0.9844859684446732 1104 44-45 0.6878784348616307 1104 46-47 0.534714317265454 1104 48-49 -0.02881253592113353 1104 50-51 -0.06539816377777896 1104 52-53 0.4381288254884552 1104 54-55 0.16230363881543752 1104 56-57 0.2954670171687397 1104 58-59 0.3381825990620442 1104 60-61 0.42121291560410157 1104 62-63 0.12742769341839022 1104 64-65 0.3866936240494141 1104 66-67 0.4974682933170165 1104 68-69 0.08393101544214332 1104 70-71 0.42749055307187334 1104 72-73 0.2622448418119667 1104 74-75 -0.1144923350417173 1104 76 0.3334014109849157 1105 1 0.2943107916548797 1105 2 -0.060325264332437456 1105 3 0.3836812777535883 1105 4 0.7942407062031123 1105 5 0.6186707862041523 1105 6 0.5605788982149171 1105 7 0.5005162811954591 1105 8 0.8218399728087604 1105 9 0.4557052111555251 1105 10-11 0.7108683375990594 1105 12-13 -0.166457909940128 1105 14-15 0.4005290692456285 1105 16-17 0.23924640024373645 1105 18-19 0.7575455356701681 1105 20-21 0.3025400005798886 1105 22-23 0.18260986660062883 1105 24-25 0.6839131298759966 1105 26-27 0.33890703411587 1105 28-29 0.5725380147820047 1105 30-31 0.7230085875970005 1105 32-33 0.22613759098855724 1105 34-35 0.8538851532784051 1105 36-37 0.0677743375353046 1105 38-39 0.5152441729114159 1105 40-41 0.4912644459647453 1105 42-43 0.5723675770183689 1105 44-45 0.5010144491511141 1105 46-47 0.6525711456532335 1105 48-49 0.5180496887609394 1105 50-51 0.49449567136479544 1105 52-53 0.6206908702940552 1105 54-55 0.6141520897393491 1105 56-57 0.648855110100726 1105 58-59 0.5183672554728176 1105 60-61 0.322909792294368 1105 62-63 0.5309296242602883 1105 64-65 0.12562115772908555 1105 66-67 1.2174207455491093 1105 68-69 0.706177836156872 1105 70-71 0.1690533400251688 1105 72-73 0.7173449059637171 1105 74-75 0.6723980660954041 1105 76 0.48164226936049204 1106 1 0.44503575512571203 1106 2 0.6179308269842352 1106 3 0.7424663863419987 1106 4 0.9063384170419724 1106 5 0.9384290068278318 1106 6 1.2368493014719455 1106 7 1.0105508746289544 1106 8 1.160256168030621 1106 9 1.4226478759738796 1106 10-11 0.36140597200765967 1106 12-13 1.039810120482116 1106 14-15 1.2531572959887356 1106 16-17 1.1639338729249076 1106 18-19 1.0232842990485764 1106 20-21 1.49256660130672 1106 22-23 0.9514770253182974 1106 24-25 1.3262332627547408 1106 26-27 1.169261835359734 1106 28-29 0.9275140991050606 1106 30-31 0.8000695194966383 1106 32-33 1.344979148067722 1106 34-35 1.184901850542154 1106 36-37 0.9995615816750316 1106 38-39 1.3019575469576026 1106 40-41 1.1691708563652554 1106 42-43 1.4437849087032077 1106 44-45 1.560903550346275 1106 46-47 1.1084239299875236 1106 48-49 1.099825068726723 1106 50-51 1.1856337578608773 1106 52-53 1.2154731314807208 1106 54-55 1.0782036426972752 1106 56-57 1.305805642739692 1106 58-59 1.2831381520297143 1106 60-61 1.2164007318120582 1106 62-63 1.2738829293693783 1106 64-65 1.5032328370716783 1106 66-67 0.710820518256515 1106 68-69 0.6266441606357809 1106 70-71 0.8413461228608234 1106 72-73 0.8953012707808234 1106 74-75 0.5646026793857786 1106 76 1.274293974679388 1107 1 0.37518370564717785 1107 2 0.09188452844218276 1107 3 0.197178435281554 1107 4 -0.36704665855023677 1107 5 0.008456225411926255 1107 6 -0.4858175807874652 1107 7 0.1491500607663383 1107 8 0.31844734489752113 1107 9 0.4647827831342539 1107 10-11 0.32177059323815627 1107 12-13 0.9609031757008069 1107 14-15 0.47667981195606757 1107 16-17 0.5147369559011707 1107 18-19 0.8954512756298172 1107 20-21 0.8351367363216795 1107 22-23 0.8556797363970716 1107 24-25 0.4704984957296503 1107 26-27 0.7214487542699075 1107 28-29 1.0066568484515415 1107 30-31 0.33767024173233295 1107 32-33 0.6022149795819871 1107 34-35 0.5751853550022261 1107 36-37 0.6741378071850406 1107 38-39 0.7266415688402645 1107 40-41 0.9225866539236662 1107 42-43 0.8424024202239409 1107 44-45 1.0933030976015061 1107 46-47 0.8754759686864304 1107 48-49 0.3616220342588292 1107 50-51 0.6377654311300702 1107 52-53 0.12577981216091416 1107 54-55 -0.039570631698389036 1107 56-57 0.20387069785058287 1107 58-59 0.5316626891790364 1107 60-61 0.1130711713517698 1107 62-63 0.17635416121242287 1107 64-65 0.5010566977987736 1107 66-67 0.8108555520776299 1107 68-69 0.4709861985262087 1107 70-71 0.5907936664509883 1107 72-73 0.396924953643893 1107 74-75 0.4181343580447887 1107 76 -0.062461893485647124 1108 1 -0.4448735549420171 1108 2 -0.06948865540712035 1108 3 0.002278373739798667 1108 4 -0.4995085963127899 1108 5 -0.225067976552058 1108 6 -0.03102401323869941 1108 7 0.4034512942771613 1108 8 -0.41547821633652404 1108 9 -0.32069068701800063 1108 10-11 0.23755872905812936 1108 12-13 0.2019996892266107 1108 14-15 0.07948798898225817 1108 16-17 -0.22030572196787546 1108 18-19 0.0030289386485762293 1108 20-21 0.18070037852326948 1108 22-23 0.30364350703362675 1108 24-25 0.17957322882573834 1108 26-27 0.23037230870760794 1108 28-29 0.18918835515423638 1108 30-31 0.09311534916283648 1108 32-33 0.022819426642698204 1108 34-35 0.285878903820894 1108 36-37 -0.13426665593004117 1108 38-39 -0.02036161527660596 1108 40-41 -0.11780346728683355 1108 42-43 0.07270236938509811 1108 44-45 -0.6870396632151241 1108 46-47 -0.07158474359249567 1108 48-49 0.009549682561079464 1108 50-51 0.016722164600757594 1108 52-53 -0.09831913627779443 1108 54-55 -0.0705354993836167 1108 56-57 0.05443126296444234 1108 58-59 -0.19461028863917562 1108 60-61 -0.0032895703601596438 1108 62-63 -0.019469026650469345 1108 64-65 0.28537291538901144 1108 66-67 0.2520965549406533 1108 68-69 0.1100304283177671 1108 70-71 0.055360332225742326 1108 72-73 -0.5464900785986337 1108 74-75 -0.13963461955936296 1108 76 0.08435532750276664 1109 1 0.1636802176853962 1109 2 0.21419391200940296 1109 3 0.10345133775434334 1109 4 0.198912463080557 1109 5 -0.0401173746532173 1109 6 0.29920454130477125 1109 7 0.17154358971506412 1109 8 0.4266148756377319 1109 9 0.425818042763467 1109 10-11 0.22350371666043145 1109 12-13 0.5882748539940081 1109 14-15 0.026807387211896128 1109 16-17 -0.011259895432935707 1109 18-19 0.20448197512223487 1109 20-21 0.25478468290293677 1109 22-23 0.5509038074049144 1109 24-25 0.11580535493542499 1109 26-27 0.07477792557362051 1109 28-29 -0.13396772742859042 1109 30-31 0.09484974283166281 1109 32-33 0.2764841362281203 1109 34-35 0.5463655618369998 1109 36-37 0.323047988776203 1109 38-39 0.5675592963720888 1109 40-41 0.4007224265682723 1109 42-43 0.9592152103219291 1109 44-45 0.42975569117932366 1109 46-47 0.2928370609477682 1109 48-49 -0.001736723646764915 1109 50-51 0.22882566293701956 1109 52-53 0.37856203848484427 1109 54-55 0.868079812100639 1109 56-57 0.9958280279990603 1109 58-59 0.35442808642667245 1109 60-61 0.46994937769795797 1109 62-63 0.8602796789779603 1109 64-65 0.7422892919194481 1109 66-67 0.6508978962050982 1109 68-69 0.6687685605684983 1109 70-71 0.7108840548769244 1109 72-73 0.3615228201513183 1109 74-75 0.8385762570160438 1109 76 0.35145195250116146 1110 1 -0.4381314002995076 1110 2 -0.24996099445597153 1110 3 0.4478687975508642 1110 4 -0.3078942320293869 1110 5 0.4871028419532806 1110 6 0.03155767553515787 1110 7 -0.1851219829793891 1110 8 0.11502970990093786 1110 9 0.5890343210699953 1110 10-11 0.17268651941724045 1110 12-13 0.1356263472660828 1110 14-15 0.4789832979437634 1110 16-17 0.26008897230581596 1110 18-19 0.16831525375695833 1110 20-21 -0.12645588158672183 1110 22-23 -0.10095047549629754 1110 24-25 -0.1885924133612562 1110 26-27 0.0025560680088076992 1110 28-29 0.3800198012881779 1110 30-31 0.4520994966879144 1110 32-33 0.11426556057993764 1110 34-35 -0.03782216379476466 1110 36-37 -0.0069421654738803795 1110 38-39 0.18066070759624608 1110 40-41 -0.08253979857120441 1110 42-43 0.13305177087329412 1110 44-45 0.49488204496993404 1110 46-47 0.20538711017651679 1110 48-49 -0.25568486936386137 1110 50-51 0.07628217754155031 1110 52-53 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0.28789203638825 2116 28-29 -0.0626877085932449 2116 30-31 -0.0414841660261871 2116 32-33 -0.5579544657705213 2116 34-35 -0.11695259857737739 2116 36-37 -0.18011739604041566 2116 38-39 0.1682891660942687 2116 40-41 -0.6905766892167904 2116 42-43 0.37009393161110893 2116 44-45 0.05096900149166572 2116 46-47 0.03459659634252432 2116 48-49 -0.3338666875456795 2116 50-51 0.4535549048142755 2116 52-53 0.026210236046722457 2116 54-55 0.22926722380994846 2116 56-57 -0.5702657301760006 2116 58-59 -0.19015544310820331 2116 60-61 -0.2841446943995649 2116 62-63 -0.0996755870713244 2116 64-65 -0.7147818115180016 2116 66-67 -0.33591082037524345 2116 68-69 0.16560317575187966 2116 70-71 0.13135703138506116 2116 72-73 -0.11478911413424697 2116 74-75 0.03411725005743094 2116 76 -0.2381729101577612 2117 1 -0.0032807035509989646 2117 2 -0.4389590037459534 2117 3 -0.30869390981476386 2117 4 -0.24290418557948712 2117 5 -0.26647380038248514 2117 6 -0.3409572547899913 2117 7 -0.17015184362968938 2117 8 -0.9895489231448487 2117 9 -0.33393847255972986 2117 10-11 -0.1011754580213804 2117 12-13 -0.2765700694558859 2117 14-15 -0.5561394625074634 2117 16-17 -0.6082255598773258 2117 18-19 -0.2842726692689226 2117 20-21 -0.38850631290723214 2117 22-23 -0.15489871703578473 2117 24-25 -0.3086985845623218 2117 26-27 -0.21916921400845268 2117 28-29 -0.4459390573714117 2117 30-31 0.09344654380138451 2117 32-33 -0.15203835448309633 2117 34-35 -0.2610338426268868 2117 36-37 -0.19564156826087498 2117 38-39 -0.4711110243214698 2117 40-41 -0.5722544634683544 2117 42-43 -0.5382023764392514 2117 44-45 -0.4503004367818946 2117 46-47 -0.3598020072753769 2117 48-49 -0.39956011820261494 2117 50-51 0.03384687561719346 2117 52-53 -0.8905886637734461 2117 54-55 0.11648556719300274 2117 56-57 -1.0068202631285033 2117 58-59 -0.19818199552542382 2117 60-61 -0.7358531467427376 2117 62-63 -0.5917019279155014 2117 64-65 -0.8268890790521155 2117 66-67 -0.04829048820555926 2117 68-69 -0.5980961789503318 2117 70-71 -0.3287085562590768 2117 72-73 -1.8789380615583475 2117 74-75 -0.6197889172968516 2117 76 -0.9485823040009862 2118 1 0.16465738985770528 2118 2 0.287373250246695 2118 3 0.3437949766349355 2118 4 0.3171638677655544 2118 5 0.18890735323147823 2118 6 0.13072377259599577 2118 7 0.17170645174378052 2118 8 0.24701877346895174 2118 9 0.6865872944124476 2118 10-11 -0.8492820383407889 2118 12-13 -0.0015028393367941817 2118 14-15 0.10073996233200688 2118 16-17 0.08240612883350451 2118 18-19 0.5082332305170425 2118 20-21 0.25618936790063174 2118 22-23 -0.1969518425502983 2118 24-25 0.23365639046649278 2118 26-27 0.2571630399841993 2118 28-29 0.0874839161207106 2118 30-31 0.11022663270978228 2118 32-33 0.2864255058977818 2118 34-35 -0.21240862996017995 2118 36-37 0.38842352147074877 2118 38-39 -0.08914790484394786 2118 40-41 -0.1056088347981401 2118 42-43 0.03789114202845667 2118 44-45 0.22488204496993092 2118 46-47 -0.26550147492260123 2118 48-49 0.17365210944680598 2118 50-51 0.23175039353608184 2118 52-53 0.16337379712332734 2118 54-55 -0.007615744480347075 2118 56-57 0.2546225775498314 2118 58-59 0.0053468997053514045 2118 60-61 0.12058996834424818 2118 62-63 -0.13379621472742542 2118 64-65 0.072485269227343 2118 66-67 -0.04064820732086716 2118 68-69 -0.16623184658657308 2118 70-71 0.22989141081188436 2118 72-73 -0.0899171516192645 2118 74-75 0.17001405729291008 2118 76 0.47889148997299813 2119 1 0.07698433267600535 2119 2 -0.46450644900142635 2119 3 -0.49749181969167466 2119 4 0.4383385757532352 2119 5 0.4099072699975608 2119 6 0.21503972853750852 2119 7 0.436307067674754 2119 8 0.5887633563283146 2119 9 -0.3835788959712616 2119 10-11 0.15367276564334276 2119 12-13 -0.15318277718880324 2119 14-15 -0.06158698048628963 2119 16-17 0.4125881336610675 2119 18-19 -0.1233318445322098 2119 20-21 -0.0903203565951145 2119 22-23 -0.4335234375895567 2119 24-25 0.39946528539754667 2119 26-27 0.557866366566337 2119 28-29 0.41045925113721893 2119 30-31 0.007141428925415028 2119 32-33 0.024342715896949585 2119 34-35 -0.19701035567668868 2119 36-37 0.1118233494539922 2119 38-39 -0.05122792038094559 2119 40-41 -0.08706848022165303 2119 42-43 -0.19792441161580143 2119 44-45 -0.2705054089046115 2119 46-47 -0.32762865634478544 2119 48-49 0.28052445636575385 2119 50-51 -0.0012421431561975282 2119 52-53 -0.1878386435725119 2119 54-55 -0.15912921450226492 2119 56-57 -0.2364644573806629 2119 58-59 0.15948223805121842 2119 60-61 -0.2635553580714216 2119 62-63 -0.026015230902551423 2119 64-65 -0.2961230597130893 2119 66-67 -0.3422948516068587 2119 68-69 -0.5682863370562892 2119 70-71 -0.7178862436563875 2119 72-73 0.08007424754284997 2119 74-75 -0.37981531318124695 2119 76 -0.46190145179100384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 21.0 13 327.0 14 1240.0 15 2081.0 16 2757.0 17 3129.0 18 3354.0 19 2953.0 20 2156.0 21 1595.0 22 1216.0 23 961.0 24 892.0 25 885.0 26 970.0 27 1086.0 28 1276.0 29 1485.0 30 1835.0 31 2189.0 32 2847.0 33 3534.0 34 4860.0 35 7186.0 36 13059.0 37 39413.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.66600844077903 18.182522166724745 10.910094087582763 33.24137530491346 2 19.727417044178573 17.954079064544857 29.667595926743328 32.65090796453324 3 20.84833701165447 19.297634258721494 26.244821311031092 33.609207418592945 4 18.23576102528362 17.119680954040344 26.74139466449839 37.90316335617764 5 17.143880435203467 28.578619274402755 28.029775041623108 26.247725248770664 6 31.173771634336163 29.91927053083982 15.473148255701396 23.433809579122624 7 29.390753862237197 26.532311147249004 16.928989042474928 27.147945948038878 8 25.139389011499592 27.738413288419096 19.96457195957719 27.15762574050412 9 23.978781894916175 16.96770821233593 18.471947961435706 40.581561931312194 10-11 27.138266155573625 21.422832694467033 23.02096643047973 28.417934719479614 12-13 25.497057343090564 18.49808340109188 23.124540209857898 32.88031904595965 14-15 25.255062531459327 19.66207844503814 21.232140782901617 33.85071824060092 16-17 26.991133310101834 20.98288612692144 21.492527200216827 30.533453362759904 18-19 26.080748828745108 20.10783288806288 21.845355635575174 31.966062647616834 20-21 25.680005420683784 20.865276648468654 22.276106400278778 31.178611530568784 22-23 27.483834746583035 20.259805629767296 21.099527626127696 31.156831997521973 24-25 26.460680683006153 21.663375537228482 20.704108103922252 31.171835675843113 26-27 25.444302474154956 22.295949974832542 22.29546598520928 29.96428156580323 28-29 26.005246447516168 21.27037596313935 23.626921438804352 29.09745615054013 30-31 26.198358307197893 20.58456266697642 23.361695125256514 29.855383900569173 32-33 24.897394199868355 21.984744647074766 23.47978859333256 29.63807255972432 34-35 24.970960622604252 21.87778294033376 23.81374143338367 29.337515003678323 36-37 25.126321291671506 20.220118480659774 24.371781469005306 30.281778758663414 38-39 24.1486622526813 20.132032369226003 26.657664459673985 29.061640918418707 40-41 23.880048011770626 20.056045998373794 26.825608858946065 29.238297130909512 42-43 22.101870135904285 20.128644441863166 26.6329809888876 31.136504433344946 44-45 21.23262477252488 19.57205637511132 29.0166298834553 30.178688968908506 46-47 21.09420374027181 21.14550664033763 28.77657103031711 28.983718589073447 48-49 20.897703953227243 21.740329887327213 27.94362488868239 29.418341270763154 50-51 20.399678630890154 23.082917102257326 26.541022960467725 29.97638130638479 52-53 19.71144538661091 23.021934409726256 26.915630928872886 30.350989274789946 54-55 19.558020676036705 22.337573082433114 26.839160568397414 31.265245673132767 56-57 20.20366283346885 22.601831416734424 26.435029232973246 30.75947651682348 58-59 20.757830952104385 22.593603593138965 25.427362837340766 31.221202617415884 60-61 20.58020676036706 21.291187516939637 26.33435939133465 31.794246331358657 62-63 21.17454601773338 21.578193363534286 25.43559066093623 31.811669957796106 64-65 21.85465721269027 21.73995111681146 24.295912687849384 32.10947898264889 66-67 21.743717814690054 22.94643202849731 22.8549579897007 32.454892167111936 68-69 21.937313663995045 23.652088899214004 23.070333372052502 31.340264064738456 70-71 22.968211561544123 23.527703566035544 23.955550392999577 29.548534479420763 72-73 24.821407829016145 22.986119177604834 23.399446315870986 28.79302667750803 74-75 23.70435977852635 24.885294459286794 23.384926627173115 28.025419135013745 76 22.758160065048205 24.921593681031478 23.777442211638984 28.542804042281332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 2.5 16 2.5 17 3.0 18 7.5 19 12.5 20 18.5 21 24.0 22 28.5 23 51.5 24 85.5 25 101.0 26 137.0 27 190.5 28 249.5 29 291.0 30 377.0 31 524.5 32 753.5 33 921.0 34 1035.0 35 1338.0 36 1634.0 37 1741.0 38 1972.5 39 2490.5 40 3045.5 41 3532.5 42 3751.0 43 3936.0 44 4237.0 45 4374.5 46 4396.0 47 4415.0 48 4269.5 49 4036.0 50 3967.0 51 3637.5 52 3114.5 53 2773.5 54 2626.0 55 2651.0 56 2658.5 57 2672.5 58 2704.0 59 2896.5 60 3238.5 61 3564.0 62 3740.0 63 3818.0 64 3936.5 65 4080.5 66 4052.0 67 3920.0 68 3758.0 69 3286.0 70 2849.0 71 2722.0 72 2516.0 73 1981.5 74 1366.5 75 1080.0 76 873.5 77 454.0 78 166.0 79 91.0 80 67.0 81 28.5 82 11.0 83 8.0 84 8.0 85 7.5 86 5.0 87 3.0 88 4.5 89 7.5 90 7.0 91 5.0 92 5.0 93 4.0 94 3.0 95 3.0 96 3.0 97 3.5 98 6.5 99 19.0 100 29.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.839896232624772E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 103308.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.36806442869866 #Duplication Level Percentage of deduplicated Percentage of total 1 79.16053610122789 57.286947767839855 2 12.780556967443355 18.49808340109188 3 4.415344693828416 9.585898478336624 4 1.6492335678553276 4.774073643861076 5 0.782483079639389 2.831339296085492 6 0.45076375698884463 1.9572540364734579 7 0.2568149594713892 1.3009641073295388 8 0.16318450549744523 0.9447477446083556 9 0.09496803188785746 0.618538738529446 >10 0.24611433616008133 2.2021527858442713 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001935958493049909 0.0 2 0.0 0.0 0.0 0.003871916986099818 0.0 3 0.0 0.0 0.0 0.0048398962326247725 0.0 4 0.0 0.0 0.0 0.010647771711774499 0.0 5 0.0 0.0 0.0 0.010647771711774499 0.0 6 0.0 0.0 0.0 0.010647771711774499 0.0 7 0.0 0.0 0.0 0.010647771711774499 0.0 8 0.0 0.0 0.0 0.010647771711774499 0.0 9 0.0 0.0 0.0 0.017423626437449182 0.0 10 0.0 0.0 0.0 0.022263522670073952 0.0 11 0.0 0.001935958493049909 0.0 0.024199481163123863 0.0 12 0.0 0.001935958493049909 0.0 0.02807139814922368 0.0 13 0.0 0.001935958493049909 0.0 0.03000735664227359 0.0 14 0.0 0.001935958493049909 0.0 0.030975335888798545 0.0 15 0.0 0.001935958493049909 0.0 0.03387927362837341 0.0 16 0.0 0.001935958493049909 0.0 0.034847252874898364 0.0 17 0.0 0.001935958493049909 0.0 0.034847252874898364 0.0 18 0.0 0.001935958493049909 0.0 0.039687149107523134 0.0 19 0.0 0.001935958493049909 0.0 0.050334920819297635 9.679792465249545E-4 20 0.0 0.001935958493049909 0.0 0.05711077554497231 9.679792465249545E-4 21 0.0 0.001935958493049909 0.0 0.060982692531072136 9.679792465249545E-4 22 0.0 0.001935958493049909 0.0 0.06388663027064699 9.679792465249545E-4 23 0.0 0.001935958493049909 0.0 0.07259844348937158 9.679792465249545E-4 24 0.0 0.001935958493049909 0.0 0.0764703604754714 9.679792465249545E-4 25 0.0 0.001935958493049909 0.0 0.08421419444767105 9.679792465249545E-4 26 0.0 0.001935958493049909 0.0 0.11131761335036977 9.679792465249545E-4 27 0.0 0.001935958493049909 0.0 0.28748983621791147 9.679792465249545E-4 28 0.0 0.001935958493049909 0.0 0.447206411894529 9.679792465249545E-4 29 0.0 0.001935958493049909 0.0 0.6301544894877453 9.679792465249545E-4 30 0.0 0.001935958493049909 0.0 0.8072946916018121 9.679792465249545E-4 31 0.0 0.001935958493049909 0.0 1.021218105083827 9.679792465249545E-4 32 0.0 0.001935958493049909 0.0 1.276764626166415 9.679792465249545E-4 33 0.0 0.001935958493049909 0.0 1.5574786076586518 9.679792465249545E-4 34 0.0 0.001935958493049909 0.0 2.0114608742788556 9.679792465249545E-4 35 0.0 0.001935958493049909 0.0 2.5380415843884307 9.679792465249545E-4 36 0.0 0.001935958493049909 0.0 3.317264877841019 9.679792465249545E-4 37 0.0 0.001935958493049909 0.0 4.36461842258102 9.679792465249545E-4 38 0.0 0.001935958493049909 0.0 5.4739226390986175 9.679792465249545E-4 39 0.0 0.001935958493049909 0.0 6.422542300693073 9.679792465249545E-4 40 0.0 0.001935958493049909 0.0 7.348898439617455 9.679792465249545E-4 41 0.0 0.001935958493049909 0.0 8.445618925930228 9.679792465249545E-4 42 0.0 0.001935958493049909 0.0 9.568474851899175 9.679792465249545E-4 43 0.0 0.001935958493049909 0.0 10.71262632129167 9.679792465249545E-4 44 0.0 0.001935958493049909 0.0 11.760947845278197 9.679792465249545E-4 45 0.0 0.001935958493049909 0.0 12.77926201262245 9.679792465249545E-4 46 0.0 0.001935958493049909 0.0 13.73078561195648 9.679792465249545E-4 47 0.0 0.001935958493049909 0.0 14.674565377318311 9.679792465249545E-4 48 0.0 0.001935958493049909 0.0 15.449916753784798 9.679792465249545E-4 49 0.0 0.001935958493049909 0.0 16.293026677508035 9.679792465249545E-4 50 0.0 0.001935958493049909 0.0 16.9938436519921 9.679792465249545E-4 51 0.0 0.001935958493049909 0.0 17.762419173732916 9.679792465249545E-4 52 0.0 0.001935958493049909 0.0 18.567777906841677 9.679792465249545E-4 53 0.0 0.001935958493049909 0.0 19.419599643783638 9.679792465249545E-4 54 0.0 0.001935958493049909 0.0 20.21140666744105 9.679792465249545E-4 55 0.0 0.001935958493049909 0.0 20.94223099856739 9.679792465249545E-4 56 0.0 0.001935958493049909 0.0 21.60820072017656 9.679792465249545E-4 57 0.0 0.001935958493049909 0.0 22.272234483292678 9.679792465249545E-4 58 0.0 0.001935958493049909 0.0 22.938204204901847 9.679792465249545E-4 59 0.0 0.001935958493049909 0.0 23.579006466101365 9.679792465249545E-4 60 0.0 0.001935958493049909 0.0 24.250784063189684 9.679792465249545E-4 61 0.0 0.001935958493049909 0.0 24.94772912068765 9.679792465249545E-4 62 0.0 0.001935958493049909 0.0 25.702752932977116 9.679792465249545E-4 63 0.0 0.001935958493049909 0.0 26.393890114995934 9.679792465249545E-4 64 0.0 0.001935958493049909 0.0 27.082123359275176 9.679792465249545E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGAGCC 15 0.002219161 70.0 18 TAAGGGA 15 0.002219161 70.0 4 TACGTGG 30 8.3264495E-6 58.333332 5 TTGGCCT 20 0.0069342298 52.5 21 GTAGAAA 20 0.0069342298 52.5 1 TGCGAAC 20 0.0069342298 52.5 5 GCCTAGG 20 0.0069342298 52.5 1 TATAAGA 35 2.0741818E-5 50.0 2 GTAAGGT 30 5.854452E-4 46.666664 19 ATAAGAG 35 0.0012504717 40.0 3 CGGAGAT 45 9.1438706E-5 38.888885 21 GACAGGC 40 0.0024092894 35.0 34 GTATAAG 40 0.0024092894 35.0 1 TATACTC 40 0.0024092894 35.0 3 GCTAAAT 40 0.0024092894 35.0 28 TAAGAGA 40 0.0024092894 35.0 4 CGCCTAA 45 0.0042905076 31.111109 25 ACAGGGC 60 4.9533276E-4 29.166666 3 ACCCCTA 60 4.9533276E-4 29.166666 7 TCTGCCG 50 0.007180504 28.000002 11 >>END_MODULE