FastQCFastQC Report
Wed 25 May 2016
SRR1780316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103308
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT95999.291632787393038No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG25872.5041623107600572No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC25722.489642622062183No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG20041.939830410036009RNA PCR Primer, Index 18 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6540.6330584272273202No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT3710.3591203004607581No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2910.2816819607387617RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT2760.26716227204088744No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1550.15003678321136796No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1310.12680528129476903No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1170.11325357184341968No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1140.11034963410384482No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGC1140.11034963410384482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT950.058.9473721
TTTATAC1000.056.0000043
CTTTATA1000.056.0000042
CTTAATA200.006934229852.52
AATCCTC200.006934229852.544
TTAATAC200.006934229852.53
GCTATTG200.006934229852.53
AGTGCGA200.006934229852.527
CTACGCG200.006934229852.548
CTCGAAA200.006934229852.557
TGATGCA200.006934229852.534
TATAAGA404.565686E-543.752
TTGGTGC350.001250471740.064
CAGCTTA350.001250471740.01
TGAGCAC350.001250471740.060
ACAAGAG350.001250471740.048
AGATGAC350.001250471740.030
GGTTGGG459.1438706E-538.88888538
GTATAAG459.1438706E-538.8888851
GAGACAG556.64023E-638.1818167
Produced by FastQC (version 0.11.5)