FastQCFastQC Report
Wed 25 May 2016
SRR1780315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386111
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATTCC250.002361917235.1662131
GTTTCTT300.00570902829.36605539
AAGTGTA454.0316547E-529.36605334
CATCAGT300.00578185829.2899951
CAGTGTA450.001387446724.4717134
ATGCAGT450.001404326924.42097931
TAAGTGT450.001405387424.41781433
TTATAGA500.00259950821.9674954
TAAGAGA755.522786E-520.5029954
ATAAGAG654.97314E-420.2776873
TATAAGA654.97314E-420.2776872
GTAAATG708.16218E-418.83903927
GGTGTAA600.00739436918.33711824
TAAATGA600.00744431218.31573528
AAATGAG600.00744431218.31573529
CTCTCTT600.007449878418.31336241
GGAACCC600.007466597518.3062486
TATAGAT600.007466597518.3062485
TGAGTGA750.001298162617.58082832
TCTAACA902.2355653E-417.085832
Produced by FastQC (version 0.11.5)