FastQCFastQC Report
Wed 25 May 2016
SRR1780315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386111
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT190534.934591348083841No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG51271.3278564972248912No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC50391.3050651237597477No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG35990.9321153761483097TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC11950.3094964919414365No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT4880.1263885255794318TruSeq Adapter, Index 5 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAGA207.8532787E-443.9962466
TATAGAG351.4437137E-743.9962465
GCTTATA603.6379788E-1240.3351173
TCTTTAT1450.036.4673581
TCTTCTG4050.036.39666744
TGGATAA250.002350306635.20155343
CTGGATA250.002350306635.20155342
CGTGATA250.002351800735.19699527
ATGCCGT26900.034.8416944
CAGCTTA756.002665E-1132.314131
TTTATAC1650.032.0014153
CTTTATA1650.032.0014152
CCCTAGT353.1862457E-431.47481
TCTTAAT651.7156708E-830.5063461
GTCTTCT4950.029.77909343
CGTCTTC5000.029.48130242
GAGCTAA300.005699137729.3764821
TCTAGCA300.00573899429.3346313
TTAAAGT300.00573899429.3346314
GTATAGA300.00573899429.3346314
Produced by FastQC (version 0.11.5)