FastQCFastQC Report
Wed 25 May 2016
SRR1780314_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780314_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147003
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA200.00694004252.512
AGTTAAT200.00694004252.565
GATGGAC501.7032506E-435.00000430
TTCGGCG601.2093569E-535.00000413
GTTAATA400.00241259935.02
GAACGAA400.00241259935.026
CGACGAA904.452886E-934.99999622
TAGATCT851.05428626E-732.94117739
ATGGACC450.004296366631.11110931
AAACGAA450.004296366631.11110918
AACGAAT450.004296366631.11110927
CGACTCA450.004296366631.11110931
ACATAAG450.004296366631.11110928
ACGAATG703.4703902E-530.028
TGGAGTC500.0071902528.00000227
CGACGCA500.0071902528.00000222
ATTCGGC500.0071902528.00000212
CGGTGGT657.9261826E-426.92307746
ACGAACG657.9261826E-426.92307724
TCTCGGT959.325282E-625.78947443