##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780314_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147003 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.560954538342756 34.0 32.0 34.0 12.0 34.0 2 30.608878730366047 34.0 32.0 34.0 12.0 34.0 3 30.3799446269804 34.0 32.0 34.0 11.0 34.0 4 30.255661449086073 34.0 31.0 34.0 11.0 34.0 5 30.27151826833466 34.0 31.0 34.0 11.0 34.0 6 33.35006768569349 38.0 34.0 38.0 21.0 38.0 7 33.51528880362986 38.0 34.0 38.0 21.0 38.0 8 33.51549968367993 38.0 34.0 38.0 21.0 38.0 9 33.830595293973595 38.0 34.0 38.0 21.0 38.0 10-11 33.58833833323129 38.0 34.0 38.0 21.0 38.0 12-13 33.6883567002034 38.0 34.0 38.0 21.0 38.0 14-15 33.634017673108715 38.0 34.0 38.0 21.0 38.0 16-17 33.691149160221215 38.0 34.0 38.0 21.0 38.0 18-19 33.69230900049659 38.0 34.0 38.0 21.0 38.0 20-21 33.69738372686272 38.0 34.0 38.0 21.0 38.0 22-23 33.69282599674837 38.0 34.0 38.0 21.0 38.0 24-25 33.72888648530983 38.0 34.0 38.0 21.0 38.0 26-27 33.71043107963783 38.0 34.0 38.0 21.0 38.0 28-29 33.81545614715346 38.0 34.5 38.0 21.0 38.0 30-31 33.82261926627348 38.0 34.0 38.0 21.0 38.0 32-33 33.884594191955266 38.0 35.0 38.0 21.0 38.0 34-35 33.84709155595464 38.0 34.5 38.0 21.0 38.0 36-37 33.753794140255636 38.0 34.0 38.0 21.0 38.0 38-39 33.76737549573818 38.0 34.0 38.0 21.0 38.0 40-41 33.86210145371183 38.0 35.0 38.0 21.0 38.0 42-43 33.90860730733387 38.0 35.0 38.0 21.0 38.0 44-45 33.98119766263274 38.0 35.0 38.0 21.0 38.0 46-47 33.91758331462623 38.0 35.0 38.0 21.0 38.0 48-49 33.86890743726318 38.0 35.0 38.0 21.0 38.0 50-51 33.82397298014327 38.0 34.5 38.0 21.0 38.0 52-53 33.85517302367978 38.0 35.0 38.0 21.0 38.0 54-55 33.85488731522486 38.0 35.0 38.0 21.0 38.0 56-57 33.78579008591662 38.0 35.0 38.0 21.0 38.0 58-59 33.85533628565403 38.0 35.0 38.0 21.0 38.0 60-61 33.86944484126174 38.0 35.0 38.0 21.0 38.0 62-63 33.75920559444366 38.0 34.5 38.0 20.5 38.0 64-65 33.69284640449514 38.0 34.5 38.0 19.5 38.0 66-67 33.56872648857507 38.0 34.0 38.0 19.0 38.0 68-69 33.507125704917584 38.0 34.0 38.0 19.0 38.0 70-71 33.4481779283416 38.0 34.0 38.0 19.0 38.0 72-73 33.39077773922981 38.0 34.0 38.0 15.0 38.0 74-75 33.392560015781996 38.0 34.0 38.0 19.0 38.0 76 31.814167057815148 37.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06299930578113333 1101 2 0.003800097392254287 1101 3 0.306733195931681 1101 4 0.1325047634806218 1101 5 0.07930599884621614 1101 6 0.1395580772931524 1101 7 0.2869322567695036 1101 8 0.034005866627900616 1101 9 0.015977171357853592 1101 10-11 0.21107544795022193 1101 12-13 0.40868013368766754 1101 14-15 -0.2024926640321283 1101 16-17 -0.0808628851781279 1101 18-19 0.18432860377901505 1101 20-21 0.12759635779279677 1101 22-23 0.030395879467199904 1101 24-25 -0.026626492114246503 1101 26-27 -0.058841538823394046 1101 28-29 -0.1590795561188898 1101 30-31 -0.11369511493007423 1101 32-33 -0.14783641186296137 1101 34-35 0.15162942039867744 1101 36-37 0.13308349784286833 1101 38-39 0.23217467720420615 1101 40-41 0.032471741448638625 1101 42-43 0.09382913839933593 1101 44-45 0.2616828187902982 1101 46-47 0.23396070777801725 1101 48-49 0.12204634252605473 1101 50-51 0.08369988659381988 1101 52-53 0.046680296005092714 1101 54-55 0.12806227502918688 1101 56-57 2.3584742923787871E-4 1101 58-59 0.18848804243872053 1101 60-61 -0.2419902399989482 1101 62-63 -0.3988582993633756 1101 64-65 -0.16559069693848727 1101 66-67 0.11891992524439132 1101 68-69 -0.1347188753051327 1101 70-71 -0.15669337886150458 1101 72-73 -0.3072494262711203 1101 74-75 -0.1779816415915363 1101 76 -0.6717178600129508 1102 1 0.03629492780715893 1102 2 0.16371756296781825 1102 3 0.3583101929699879 1102 4 0.5568622442155728 1102 5 -0.20409026464633584 1102 6 0.550096656521994 1102 7 -0.5248317060057843 1102 8 0.31718456488597013 1102 9 0.4195326083832427 1102 10-11 0.4941348362409954 1102 12-13 0.35682591643123374 1102 14-15 0.23796113493759918 1102 16-17 0.3905904505881992 1102 18-19 -0.06942264195740933 1102 20-21 0.2958339390288529 1102 22-23 -0.045653697333335685 1102 24-25 0.05082673136033833 1102 26-27 -0.005404709941963404 1102 28-29 0.24856400184469862 1102 30-31 0.4179150081697358 1102 32-33 0.26893786771538686 1102 34-35 0.1559666886215183 1102 36-37 0.2821437563945821 1102 38-39 0.33262665143325165 1102 40-41 0.011627588338861017 1102 42-43 0.06596324280257448 1102 44-45 0.09549105258168566 1102 46-47 -0.43287322678396833 1102 48-49 -0.08102273092677592 1102 50-51 0.013303496773062307 1102 52-53 0.24137661704716606 1102 54-55 -0.2852976340145119 1102 56-57 0.06224895748917447 1102 58-59 0.14000959543339775 1102 60-61 -0.0026228635549543355 1102 62-63 -0.2715756189596803 1102 64-65 0.11254189152634098 1102 66-67 -0.0911568542286787 1102 68-69 0.06538850366543159 1102 70-71 0.037897668680002994 1102 72-73 -0.006662000138028645 1102 74-75 -0.21804522678587546 1102 76 0.07050242829607356 1103 1 -0.05494274726659043 1103 2 0.21752201190076548 1103 3 0.033397231532720895 1103 4 0.28540823512635427 1103 5 -0.3486141437756878 1103 6 0.20874603064164177 1103 7 0.034261498342154084 1103 8 0.28715293866012104 1103 9 0.14212542736960643 1103 10-11 0.6191979558093976 1103 12-13 0.3975480042936752 1103 14-15 0.021088942097350127 1103 16-17 0.47088518575177574 1103 18-19 0.6445394065715675 1103 20-21 0.6617480432625058 1103 22-23 -0.03504960984297156 1103 24-25 0.41086074948562157 1103 26-27 0.1991026916579557 1103 28-29 0.18655400902039787 1103 30-31 0.04453286237018261 1103 32-33 0.36904550318991625 1103 34-35 0.047228282689602 1103 36-37 0.11962083402500667 1103 38-39 -0.05961163490368904 1103 40-41 0.2277024813677997 1103 42-43 0.05236263685471698 1103 44-45 0.1974723426127838 1103 46-47 0.3281959522629947 1103 48-49 0.5099425333781156 1103 50-51 -0.15931191237462627 1103 52-53 0.5691997227957017 1103 54-55 0.46873537419767786 1103 56-57 0.5495785346545006 1103 58-59 0.12196404303750796 1103 60-61 0.4078137909750481 1103 62-63 0.22707561552619637 1103 64-65 0.3100662756122361 1103 66-67 0.008306297231705173 1103 68-69 0.42034637965873145 1103 70-71 0.20188230737030466 1103 72-73 0.43367339731593546 1103 74-75 0.21192527312111054 1103 76 0.13936255533251085 1104 1 0.6392973853346717 1104 2 -0.05099554740721857 1104 3 0.35189925163010116 1104 4 0.6857889457862534 1104 5 0.3857430508788262 1104 6 0.5941184537225439 1104 7 0.27168768709177016 1104 8 -0.21137083633210807 1104 9 -0.07472425666644256 1104 10-11 0.19688753417881344 1104 12-13 -0.0025607701805796523 1104 14-15 0.03484114348551515 1104 16-17 0.03288663607810349 1104 18-19 0.34299455005027824 1104 20-21 -0.09134684951693828 1104 22-23 0.2616975154030712 1104 24-25 0.09813413599758292 1104 26-27 0.14420395551375265 1104 28-29 0.546752910916176 1104 30-31 0.5301331938733469 1104 32-33 0.20971679708818414 1104 34-35 0.3195658979387517 1104 36-37 0.36009011818537573 1104 38-39 0.2861940731663424 1104 40-41 0.3276001815646339 1104 42-43 0.45274334751461964 1104 44-45 0.5710025922760948 1104 46-47 0.36126209750280935 1104 48-49 0.11527895728777793 1104 50-51 0.11744790322673992 1104 52-53 -0.05462188706651716 1104 54-55 0.0852147070475695 1104 56-57 0.33586259142441577 1104 58-59 -0.01583638170090751 1104 60-61 0.22167994657434065 1104 62-63 0.06381678407483093 1104 64-65 0.32156529146222823 1104 66-67 0.31102954389077553 1104 68-69 0.12927754095574073 1104 70-71 0.061090584589820196 1104 72-73 -0.05068379733857853 1104 74-75 0.37848618682669155 1104 76 -0.3913791829871833 1105 1 0.4259393148337445 1105 2 -0.13615324685063257 1105 3 0.04855973400486846 1105 4 0.11208764708495522 1105 5 0.37711596368031053 1105 6 0.6627640937967598 1105 7 0.711715516405313 1105 8 0.009501148825592054 1105 9 0.28492323869534175 1105 10-11 -0.0443230409603359 1105 12-13 -0.5265867442065542 1105 14-15 -0.027589283230618378 1105 16-17 0.15886066205212757 1105 18-19 0.06681180422467747 1105 20-21 -0.01996466027760846 1105 22-23 0.16874760816744327 1105 24-25 0.015666603530050338 1105 26-27 0.29367056033749606 1105 28-29 0.27588092575847867 1105 30-31 0.12336139424440518 1105 32-33 -0.18045017879308034 1105 34-35 0.22879595359737692 1105 36-37 0.3753034762558727 1105 38-39 0.5351968560976985 1105 40-41 0.5750259700618443 1105 42-43 0.31150958128085193 1105 44-45 0.4154135384727624 1105 46-47 0.41411460214103357 1105 48-49 0.2568605899408354 1105 50-51 0.29186812586124944 1105 52-53 -0.11436214680678347 1105 54-55 0.1465783434112069 1105 56-57 0.3541371739847179 1105 58-59 0.36980833072951214 1105 60-61 0.2231641766300001 1105 62-63 0.38684554103215874 1105 64-65 0.04002540277948441 1105 66-67 0.32584865335274316 1105 68-69 0.3867218823286507 1105 70-71 0.42108594637090135 1105 72-73 0.3709303028469506 1105 74-75 0.43871345955396635 1105 76 0.28682118807033063 1106 1 0.028536717431148162 1106 2 -0.024464935162320955 1106 3 0.47453375997889324 1106 4 0.62897076396807 1106 5 -0.14236455580021 1106 6 0.48873811977078674 1106 7 -0.3350377303479348 1106 8 0.03807257739702408 1106 9 0.6953128490849494 1106 10-11 0.22840423176693747 1106 12-13 0.5136729960531881 1106 14-15 0.5009821453408065 1106 16-17 0.2835359867274505 1106 18-19 0.620058557471431 1106 20-21 0.6826327423197966 1106 22-23 0.6103060497258852 1106 24-25 0.5247575126209654 1106 26-27 0.7547095213764621 1106 28-29 0.33951728939484127 1106 30-31 0.09738736827037542 1106 32-33 0.31954982120691966 1106 34-35 0.21451023931166446 1106 36-37 0.3947839957363968 1106 38-39 0.49233971324055403 1106 40-41 0.3932077882436644 1106 42-43 0.22839269816397234 1106 44-45 0.6011278241870457 1106 46-47 0.4426860307124585 1106 48-49 0.4036138366940847 1106 50-51 0.3256343596274931 1106 52-53 0.4233001908555565 1106 54-55 0.29982509665795476 1106 56-57 0.14764366749120938 1106 58-59 0.3607174216386042 1106 60-61 0.617969371435187 1106 62-63 0.33619619038280746 1106 64-65 0.5802851430392266 1106 66-67 0.11026423776832672 1106 68-69 0.5087998044065785 1106 70-71 0.543163868448822 1106 72-73 0.5527484846651234 1106 74-75 0.06858358942409382 1106 76 0.9491588504079935 1107 1 -0.8565786853008355 1107 2 -0.6841244466353054 1107 3 -0.19351684226860044 1107 4 -0.577812090483178 1107 5 -0.28003362248482233 1107 6 -0.9278758362214106 1107 7 -0.6463492167889555 1107 8 -0.9607363856606455 1107 9 -0.5677717347250208 1107 10-11 -0.6727262439749353 1107 12-13 -0.2743964474566738 1107 14-15 -0.14195911029474928 1107 16-17 0.1255957404452417 1107 18-19 -0.18272052872734434 1107 20-21 0.023528375184341144 1107 22-23 -0.38271931868808196 1107 24-25 -0.32062974261084776 1107 26-27 -0.21302481682419483 1107 28-29 -0.2751250630713358 1107 30-31 -0.4725103505910724 1107 32-33 -0.2665177381767023 1107 34-35 -0.11850907298226332 1107 36-37 -0.18722932772770662 1107 38-39 -0.19374803320988576 1107 40-41 0.11666515988756743 1107 42-43 -0.07640173020558905 1107 44-45 -0.5701560732626518 1107 46-47 -0.27971151992343124 1107 48-49 0.016853188016334286 1107 50-51 0.22659324530139457 1107 52-53 -0.34052122089331505 1107 54-55 -0.13064391619447235 1107 56-57 0.11795522122245927 1107 58-59 -0.2551630709258248 1107 60-61 -0.1591755044143497 1107 62-63 -0.5060587049808944 1107 64-65 0.10418663015314422 1107 66-67 -0.056019996132484096 1107 68-69 0.09289576026419155 1107 70-71 -0.08635971677424692 1107 72-73 0.07623277239812154 1107 74-75 -0.2202832432251256 1107 76 -0.21608574955163107 1108 1 -0.09942645863329602 1108 2 -0.24037813588564916 1108 3 0.27029243168693284 1108 4 0.32977628755840627 1108 5 0.30724912166157026 1108 6 -0.05842610840610263 1108 7 0.2582221743043718 1108 8 0.18895207764361288 1108 9 -0.4629314382732659 1108 10-11 0.030015278947601587 1108 12-13 -0.13176511014441417 1108 14-15 0.24591063457309303 1108 16-17 0.03125914964049059 1108 18-19 0.06631862651631337 1108 20-21 0.12784069892016703 1108 22-23 0.07593156345266294 1108 24-25 0.2786947146594301 1108 26-27 -0.048972872179348315 1108 28-29 0.30402493364932326 1108 30-31 0.1358552295230382 1108 32-33 0.25443392444545765 1108 34-35 0.48424556727484 1108 36-37 0.28574240441632526 1108 38-39 0.40677338083877856 1108 40-41 0.11231020481443466 1108 42-43 0.3549585373880362 1108 44-45 0.2678218896152842 1108 46-47 0.5174846498148824 1108 48-49 0.1886869913874918 1108 50-51 0.45652372342824066 1108 52-53 0.5401339899678419 1108 54-55 0.353976009036721 1108 56-57 0.2322072052193036 1108 58-59 -0.1235830879783606 1108 60-61 0.17169286313336585 1108 62-63 0.2042875926514256 1108 64-65 0.2252465107854036 1108 66-67 0.5194866609148008 1108 68-69 0.17869952493921204 1108 70-71 0.6179460482942929 1108 72-73 0.12148101795462196 1108 74-75 0.15712542733302115 1108 76 0.05335086092911823 1109 1 0.6311341200285483 1109 2 0.4716389609415721 1109 3 0.24596233140746548 1109 4 0.005594140591448138 1109 5 0.023869210433556276 1109 6 0.530296561329223 1109 7 0.2961311008208938 1109 8 0.09002062934507649 1109 9 0.08752064129274117 1109 10-11 0.46606656942927316 1109 12-13 0.10198468436487929 1109 14-15 0.5204626648213306 1109 16-17 0.3458736490651191 1109 18-19 0.3940845314974055 1109 20-21 0.022892482579536022 1109 22-23 0.3012151406349801 1109 24-25 -0.10381391595046807 1109 26-27 0.43133289799983743 1109 28-29 -0.12931387943632444 1109 30-31 0.23504970593270968 1109 32-33 0.13383553549263638 1109 34-35 0.3300946548960795 1109 36-37 0.010368411320811788 1109 38-39 0.28324880414965037 1109 40-41 0.2373636323995072 1109 42-43 0.4296913994626763 1109 44-45 0.0699589930182114 1109 46-47 0.12969901772545 1109 48-49 0.3477696808499289 1109 50-51 0.5347252687183968 1109 52-53 0.3090144765698426 1109 54-55 0.28294197977484714 1109 56-57 0.07231899216653659 1109 58-59 0.3840940450152317 1109 60-61 0.09329404675986552 1109 62-63 0.21671567090228194 1109 64-65 0.20496046771454957 1109 66-67 0.631043458547552 1109 68-69 0.5932153888221592 1109 70-71 0.38082620611115914 1109 72-73 0.1722290041456489 1109 74-75 0.3062459270864295 1109 76 0.260847162096308 1110 1 0.09579577416388929 1110 2 -0.2119336068415194 1110 3 -0.062171639884400776 1110 4 -0.2944742121084829 1110 5 0.03817770895258121 1110 6 0.17700423961585443 1110 7 0.2481019370022608 1110 8 0.09931659653577896 1110 9 -0.010724972742345074 1110 10-11 0.003569417458436419 1110 12-13 0.16347933007004656 1110 14-15 0.1427000765324209 1110 16-17 0.27975380399789884 1110 18-19 0.03555183612260038 1110 20-21 -0.22288104214135274 1110 22-23 -0.032072624231737734 1110 24-25 0.032344662313377626 1110 26-27 -0.07520617696028609 1110 28-29 -0.18686685277877046 1110 30-31 -0.2164654455824362 1110 32-33 0.13255961842724417 1110 34-35 0.02243915250373618 1110 36-37 0.11232785538552292 1110 38-39 0.3416675783578924 1110 40-41 -0.011394626885063985 1110 42-43 -0.042557404365076934 1110 44-45 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-0.5161792646777457 2116 16-17 -0.7209398945341405 2116 18-19 -0.6914442254599251 2116 20-21 -0.9242550127822398 2116 22-23 -0.687282076433668 2116 24-25 -0.9783964762473119 2116 26-27 -0.5114082893842067 2116 28-29 -0.872889946226671 2116 30-31 -0.5349178265348229 2116 32-33 -0.5759974886261077 2116 34-35 -0.7607912449183942 2116 36-37 -1.1705221267125836 2116 38-39 -0.785234498262227 2116 40-41 -1.123930430680268 2116 42-43 -0.5663893055466005 2116 44-45 -0.5284872036422641 2116 46-47 -0.6221562698441261 2116 48-49 -0.5939047161816049 2116 50-51 -0.7550010763650903 2116 52-53 -0.9793667850257037 2116 54-55 -0.8650403949932439 2116 56-57 -1.1960251636776178 2116 58-59 -1.1293567898641754 2116 60-61 -0.7196465840379105 2116 62-63 -0.961693420006803 2116 64-65 -1.1950163411908292 2116 66-67 -0.8445827010071767 2116 68-69 -0.8528653161871134 2116 70-71 -0.8381441092877253 2116 72-73 -1.0149695116242938 2116 74-75 -0.7031464662345286 2116 76 0.06525347007404392 2117 1 -0.6362915134639238 2117 2 -0.1748872634766805 2117 3 -0.28502955588262324 2117 4 -0.11209023159164033 2117 5 0.10040912296318538 2117 6 0.5343629319765739 2117 7 0.20309868336688197 2117 8 -0.32480927083187083 2117 9 -0.3643339308703162 2117 10-11 0.0861279990022723 2117 12-13 -0.37136356121988 2117 14-15 -0.008532761346337736 2117 16-17 -0.4616047337346032 2117 18-19 -0.32978953787795007 2117 20-21 -0.784352200757688 2117 22-23 -0.540673818734696 2117 24-25 -0.2754428012040293 2117 26-27 -0.5670946384739679 2117 28-29 -0.6000914309336665 2117 30-31 -0.2468853256167165 2117 32-33 -0.5066412114108445 2117 34-35 -0.31809083570586694 2117 36-37 -0.5849809693756143 2117 38-39 -0.23598800017713017 2117 40-41 -0.2635578111954544 2117 42-43 0.11373306473826972 2117 44-45 -0.5208833266158805 2117 46-47 -0.42461941448501506 2117 48-49 -0.6160736737389314 2117 50-51 -0.21528975481847823 2117 52-53 -0.37834572107313136 2117 54-55 -0.20954291322419039 2117 56-57 -0.6501428647426195 2117 58-59 -0.2753223740412949 2117 60-61 -0.42293471162827956 2117 62-63 -0.42586437116361964 2117 64-65 -0.9353860752026861 2117 66-67 -0.5098973487171676 2117 68-69 -0.8347884839117867 2117 70-71 -0.5300608502771809 2117 72-73 -2.0751185069550786 2117 74-75 -0.8388981319393736 2117 76 -0.984356991077 2118 1 -0.12600873711430793 2118 2 0.1526631181315352 2118 3 -0.1727914536675641 2118 4 -0.8498328158717783 2118 5 0.14742751741155402 2118 6 -0.6040416393302195 2118 7 -0.06344766238481014 2118 8 0.18434133454660895 2118 9 -0.028911631098083035 2118 10-11 -1.0781666584827079 2118 12-13 -0.2096296080784299 2118 14-15 -0.3893684367196144 2118 16-17 -0.09590685022833867 2118 18-19 -0.3472283007480712 2118 20-21 -0.1098913139348241 2118 22-23 -0.18194211661553794 2118 24-25 -0.2584535768077387 2118 26-27 0.05640523498927763 2118 28-29 -0.2168349076309255 2118 30-31 -0.029505169243925877 2118 32-33 -0.4854296552751478 2118 34-35 0.0576567301265527 2118 36-37 -0.1483692241007617 2118 38-39 -0.8595511420181765 2118 40-41 0.13220920133834113 2118 42-43 -0.06733437269263476 2118 44-45 -0.3123840525372614 2118 46-47 -0.1216804318405309 2118 48-49 -0.3704525114655226 2118 50-51 -0.26304406039808015 2118 52-53 -0.14622070275859755 2118 54-55 -0.273130962826599 2118 56-57 -0.1501794938034493 2118 58-59 -0.12822006574447897 2118 60-61 -0.2459630691811867 2118 62-63 -0.006058704980894447 2118 64-65 -0.15617606414736684 2118 66-67 0.14993855309031545 2118 68-69 -0.5625446542435881 2118 70-71 0.019857899806062562 2118 72-73 0.23944520763128452 2118 74-75 0.11779965832928241 2118 76 0.13106450951572768 2119 1 0.6836359329364541 2119 2 0.1413798799210717 2119 3 -0.28639972278200077 2119 4 0.22449452954259996 2119 5 0.16915235368624337 2119 6 0.14529425399189222 2119 7 0.5114188587482502 2119 8 0.7693864411981934 2119 9 0.8451711126487993 2119 10-11 0.3780812045403579 2119 12-13 0.1830809992175304 2119 14-15 0.4754036629095353 2119 16-17 0.35794102331522737 2119 18-19 0.22321960961725296 2119 20-21 0.38890540366860193 2119 22-23 0.3101280551316492 2119 24-25 0.28649526725212127 2119 26-27 0.02016868150567319 2119 28-29 0.3769093389696323 2119 30-31 0.008999867446327414 2119 32-33 0.35000997019498215 2119 34-35 0.15890340959315807 2119 36-37 0.2909406309793283 2119 38-39 0.05460156306585873 2119 40-41 0.1350628874591706 2119 42-43 -0.12530233776457322 2119 44-45 0.014731225861517316 2119 46-47 -0.013174606112642095 2119 48-49 0.006135426779131592 2119 50-51 0.005662706914712601 2119 52-53 -0.09323682542765255 2119 54-55 0.05376075490833898 2119 56-57 0.2082079762788709 2119 58-59 0.42653257095629726 2119 60-61 0.19842292803087958 2119 62-63 0.26768475258203495 2119 64-65 0.1311322654599465 2119 66-67 -0.03357187535712569 2119 68-69 -0.53904448328543 2119 70-71 -0.31715322562462234 2119 72-73 0.3727596917321776 2119 74-75 0.024108014237860687 2119 76 -0.061145058880686776 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 17.0 13 240.0 14 1065.0 15 1912.0 16 2533.0 17 2835.0 18 2986.0 19 2613.0 20 2024.0 21 1610.0 22 1168.0 23 1018.0 24 987.0 25 1005.0 26 1136.0 27 1353.0 28 1590.0 29 1992.0 30 2448.0 31 2969.0 32 3905.0 33 4959.0 34 6905.0 35 10154.0 36 19144.0 37 68435.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.7705965184384 21.337659775650835 10.390264144269166 27.501479561641602 2 21.03970667265294 23.24442358319218 28.975599137432567 26.740270606722312 3 19.222056692720557 22.19954694802147 29.36538710094352 29.213009258314454 4 16.581974517526852 19.041788262824568 30.19734291136916 34.178894308279425 5 17.097610252851982 30.523866859860004 30.942905927089924 21.435616960198093 6 28.317789432868718 32.95987156724693 18.39486268987708 20.327476310007278 7 27.62664707522976 26.498778936484293 20.192785181254806 25.681788807031147 8 24.538274729087163 28.813017421413168 21.19684632286416 25.45186152663551 9 23.334897927253184 17.547260940252922 21.651939076073276 37.46590205642062 10-11 26.423270273395783 22.83048645265743 24.696094637524403 26.05014863642239 12-13 24.164132704774733 20.79923538975395 25.17941810711346 29.857213798357858 14-15 23.246804486983258 22.06893736862513 23.241362421175076 31.442895723216534 16-17 24.99608851520037 23.8291055284586 23.48251396230009 27.69229199404094 18-19 24.39167908137929 23.141364461949756 24.24202227165432 28.224934185016632 20-21 24.56922647837119 23.536934620381896 24.08998455813827 27.803854343108643 22-23 25.53621354666231 23.206669251647927 23.55870288361462 27.698414318075145 24-25 24.834527186520003 24.076719522730826 23.169935307442703 27.918817983306464 26-27 24.340999843540608 24.062774229097368 24.119575790970252 27.476650136391772 28-29 24.57534880240539 23.65870084283994 24.974660381080657 26.791289973674004 30-31 24.730107548825533 23.12945994299436 24.761399427222575 27.379033080957534 32-33 23.51720713182724 24.070597198696625 24.97057883172452 27.44161683775161 34-35 24.0277409304572 23.359387223389998 25.372611443303878 27.24026040284892 36-37 24.321952613211977 22.662462670829846 25.438596491228072 27.57698822473011 38-39 23.226056611089568 22.65327918477854 27.018496221165556 27.102167982966336 40-41 22.87232233355782 22.35226491976354 27.376312048053443 27.3991006986252 42-43 22.030842907967866 22.32335394515758 27.68276837887662 27.96303476799793 44-45 21.424732828581732 22.450222104310797 28.538533227213048 27.586511839894424 46-47 21.2101113582716 23.436936661156572 28.155887975075338 27.197064005496486 48-49 20.94004884254063 23.699176207288286 27.962694638884923 27.398080311286165 50-51 20.738012149411915 24.32297300055101 27.21951252695523 27.71950232308184 52-53 20.193125310367815 24.378414045971848 27.534472085603696 27.893988558056638 54-55 20.19482595593287 23.923321292762733 27.434474126378372 28.447378624926024 56-57 20.693455235607434 24.075699135391794 26.910675292340976 28.320170336659796 58-59 21.15501044196377 23.96413678632409 26.33143541288273 28.54941735882941 60-61 21.15262953817269 23.10599103419658 26.72020298905465 29.021176438576084 62-63 21.286640408699142 23.292041659013762 26.387896845642604 29.03342108664449 64-65 21.770304007401208 23.585573083542513 25.65525873621627 28.98886417284001 66-67 21.65738114188146 24.190322646476602 24.907995074930444 29.2443011367115 68-69 22.130500738080176 24.603239389672318 24.902553009122265 28.363706863125245 70-71 22.912457568893153 24.30834744869152 25.193703529859935 27.585491452555388 72-73 23.803255715869746 24.31515003095175 24.962755862125263 26.918838391053246 74-75 22.936266606803944 25.16003074767182 25.322612463691218 26.581090181833023 76 22.761595080339042 25.05680194827281 25.320063672603094 26.861539298785054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 1.0 9 1.0 10 1.5 11 1.5 12 1.0 13 0.5 14 3.5 15 12.5 16 19.0 17 20.0 18 32.5 19 57.5 20 87.5 21 105.0 22 137.0 23 206.5 24 310.5 25 377.0 26 456.5 27 649.5 28 884.0 29 1005.0 30 1164.0 31 1532.0 32 1985.5 33 2230.0 34 2481.0 35 3050.0 36 3525.0 37 3682.0 38 4129.5 39 4869.5 40 5636.0 41 6279.0 42 6448.0 43 6596.0 44 6981.0 45 7186.5 46 7155.0 47 7193.0 48 6871.0 49 6234.5 50 5958.0 51 5591.0 52 4783.0 53 4061.5 54 3781.0 55 3717.5 56 3426.5 57 3168.5 58 3138.0 59 3136.0 60 3303.5 61 3527.0 62 3581.0 63 3597.5 64 3700.0 65 3650.5 66 3520.5 67 3526.0 68 3446.0 69 3240.5 70 2958.0 71 2801.0 72 2636.0 73 2248.0 74 1750.0 75 1475.0 76 1256.0 77 831.0 78 465.0 79 305.0 80 207.5 81 70.0 82 20.5 83 11.0 84 9.5 85 8.5 86 9.5 87 10.0 88 7.0 89 4.0 90 6.0 91 6.0 92 4.0 93 6.0 94 5.5 95 4.5 96 6.0 97 5.5 98 6.0 99 20.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 6.802582260225982E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147003.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.13406529118454 #Duplication Level Percentage of deduplicated Percentage of total 1 74.13846137527975 47.548009224301545 2 14.673469171289472 18.821384597593248 3 5.429629079646581 10.44672557702904 4 2.171215222902237 5.569954354673034 5 1.1412933951357143 3.6597892560015786 6 0.6968678072529407 2.681577926981082 7 0.4974596675823884 2.2332877560321895 8 0.31184038863373603 1.599967347605151 9 0.2344106322722982 1.3530336115589479 >10 0.7053532600048792 6.086270348224186 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0013605164520451964 0.0 2 0.0 0.0 0.0 0.0013605164520451964 0.0 3 0.0 0.0 0.0 0.0020407746780677946 0.0 4 0.0 0.0 0.0 0.008843356938293776 0.0 5 0.0 0.0 0.0 0.011564389842384169 0.0 6 0.0 6.802582260225982E-4 0.0 0.012244648068406767 0.0 7 0.0 6.802582260225982E-4 0.0 0.012244648068406767 0.0 8 0.0 6.802582260225982E-4 0.0 0.012924906294429366 0.0 9 0.0 6.802582260225982E-4 0.0 0.017686713876587552 0.0 10 0.0 6.802582260225982E-4 0.0 0.019727488554655347 0.0 11 0.0 6.802582260225982E-4 0.0 0.021088005006700543 0.0 12 0.0 6.802582260225982E-4 0.0 0.02244852145874574 0.0 13 0.0 6.802582260225982E-4 0.0 0.025849812588858732 0.0 14 0.0 6.802582260225982E-4 0.0 0.03061162017101692 0.0 15 0.0 6.802582260225982E-4 0.0 0.032652394849084714 0.0 16 0.0 6.802582260225982E-4 0.0 0.032652394849084714 0.0 17 0.0 6.802582260225982E-4 0.0 0.034693169527152505 0.0 18 0.0 6.802582260225982E-4 0.0 0.0374142024312429 0.0 19 0.0 6.802582260225982E-4 0.0 0.04897859227362707 0.0 20 0.0 6.802582260225982E-4 0.0 0.05578117453385305 0.0 21 0.0 6.802582260225982E-4 0.0 0.06054298211601124 0.0 22 0.0 6.802582260225982E-4 0.0 0.07006659728032762 0.0 23 0.0 6.802582260225982E-4 0.0 0.0782296959925988 0.0 24 0.0 6.802582260225982E-4 0.0 0.08911382760896036 0.0 25 0.0 6.802582260225982E-4 0.0 0.10884131616361571 0.0 26 0.0 6.802582260225982E-4 0.0 0.16870404005360434 0.0 27 0.0 6.802582260225982E-4 0.0 0.2782256144432427 0.0 28 0.0 6.802582260225982E-4 0.0 0.39250899641503917 0.0 29 0.0 6.802582260225982E-4 0.0 0.519717284681265 0.0 30 0.0 6.802582260225982E-4 0.0 0.668693836180214 0.0 31 0.0 6.802582260225982E-4 0.0 0.873451562213016 0.0 32 0.0 6.802582260225982E-4 0.0 1.1387522703618294 0.0 33 0.0 6.802582260225982E-4 0.0 1.4625551859485861 0.0 34 0.0 6.802582260225982E-4 0.0 1.9326136201302013 0.0 35 0.0 6.802582260225982E-4 0.0 2.506751562893274 0.0 36 0.0 6.802582260225982E-4 0.0 3.2346278647374542 0.0 37 0.0 6.802582260225982E-4 0.0 4.08427038903968 0.0 38 0.0 6.802582260225982E-4 0.0 4.931191880437814 0.0 39 0.0 6.802582260225982E-4 0.0 5.775392338931859 0.0 40 0.0 6.802582260225982E-4 0.0 6.637959769528513 0.0 41 0.0 6.802582260225982E-4 0.0 7.447467058495405 0.0 42 0.0 6.802582260225982E-4 0.0 8.242008666489799 0.0 43 0.0 6.802582260225982E-4 0.0 9.103215580634409 0.0 44 0.0 6.802582260225982E-4 0.0 9.890954606368577 0.0 45 0.0 6.802582260225982E-4 0.0 10.809303211499085 0.0 46 0.0 6.802582260225982E-4 0.0 11.611327659979729 0.0 47 0.0 6.802582260225982E-4 0.0 12.44192295395332 0.0 48 0.0 6.802582260225982E-4 0.0 13.173200546927614 0.0 49 0.0 6.802582260225982E-4 0.0 13.85277851472419 0.0 50 0.0 0.0013605164520451964 0.0 14.54119983945906 0.0 51 0.0 0.0013605164520451964 0.0 15.230981680645973 0.0 52 0.0 0.0013605164520451964 0.0 15.87450596246335 0.0 53 0.0 0.0013605164520451964 0.0 16.47857526717142 0.0 54 0.0 0.0013605164520451964 0.0 17.128221873022998 0.0 55 0.0 0.0013605164520451964 0.0 17.73093066127902 0.0 56 0.0 0.0013605164520451964 0.0 18.376495717774468 0.0 57 0.0 0.0013605164520451964 0.0 19.035665938790366 0.0 58 0.0 0.0013605164520451964 0.0 19.669666605443425 0.0 59 0.0 0.0013605164520451964 0.0 20.290062107576034 0.0 60 0.0 0.0013605164520451964 0.0 20.892770895832058 0.0 61 0.0 0.0013605164520451964 0.0 21.494119167636036 0.0 62 0.0 0.0013605164520451964 0.0 22.096147697666034 0.0 63 0.0 0.0013605164520451964 0.0 22.658721250586723 0.0 64 0.0 0.0013605164520451964 0.0 23.280477269171378 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCCTA 20 0.006940042 52.5 12 AGTTAAT 20 0.006940042 52.5 65 GATGGAC 50 1.7032506E-4 35.000004 30 TTCGGCG 60 1.2093569E-5 35.000004 13 GTTAATA 40 0.002412599 35.0 2 GAACGAA 40 0.002412599 35.0 26 CGACGAA 90 4.452886E-9 34.999996 22 TAGATCT 85 1.05428626E-7 32.941177 39 ATGGACC 45 0.0042963666 31.111109 31 AAACGAA 45 0.0042963666 31.111109 18 AACGAAT 45 0.0042963666 31.111109 27 CGACTCA 45 0.0042963666 31.111109 31 ACATAAG 45 0.0042963666 31.111109 28 ACGAATG 70 3.4703902E-5 30.0 28 TGGAGTC 50 0.00719025 28.000002 27 CGACGCA 50 0.00719025 28.000002 22 ATTCGGC 50 0.00719025 28.000002 12 CGGTGGT 65 7.9261826E-4 26.923077 46 ACGAACG 65 7.9261826E-4 26.923077 24 TCTCGGT 95 9.325282E-6 25.789474 43 >>END_MODULE