FastQCFastQC Report
Wed 25 May 2016
SRR1780314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147003
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT101006.870608082828242No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG26821.8244525621926084No Hit
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG17451.1870506044094338Illumina PCR Primer Index 10 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC11200.76188921314531No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3920.26666122460085845No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT3430.23332857152575118Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT2470.16802378182758176No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1540.10475976680748011No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCT150.002221035870.0000163
TAACTCG150.002221035870.0000136
ACTCGTA252.3841597E-456.00000438
TCCCGTA252.3841597E-456.00000412
GGACACC252.3841597E-456.00000419
CTGTAGG200.00694004252.52
CAATGTA200.00694004252.545
TGGACAG350.001252200340.032
AACTCGT350.001252200340.037
CCGTCTT17900.036.17318747
GCCGTCT18050.036.0664846
GTCTTCT18050.035.87257449
CGTATGC18250.035.86301441
TATGCCG18250.035.86301443
TCGTATG18250.035.86301440
TCTTCTG18000.035.7777850
TCTCGTA17650.035.6940538
CTTGAAA18050.035.6786757
CTCGTAT18150.035.6749339
CGTCTTC18150.035.6749348