Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780314_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 147003 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT | 10100 | 6.870608082828242 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG | 2682 | 1.8244525621926084 | No Hit |
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG | 1745 | 1.1870506044094338 | Illumina PCR Primer Index 10 (96% over 26bp) |
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC | 1120 | 0.76188921314531 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC | 392 | 0.26666122460085845 | No Hit |
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT | 343 | 0.23332857152575118 | Illumina PCR Primer Index 10 (95% over 23bp) |
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT | 247 | 0.16802378182758176 | No Hit |
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG | 154 | 0.10475976680748011 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTCGCT | 15 | 0.0022210358 | 70.00001 | 63 |
TAACTCG | 15 | 0.0022210358 | 70.00001 | 36 |
ACTCGTA | 25 | 2.3841597E-4 | 56.000004 | 38 |
TCCCGTA | 25 | 2.3841597E-4 | 56.000004 | 12 |
GGACACC | 25 | 2.3841597E-4 | 56.000004 | 19 |
CTGTAGG | 20 | 0.006940042 | 52.5 | 2 |
CAATGTA | 20 | 0.006940042 | 52.5 | 45 |
TGGACAG | 35 | 0.0012522003 | 40.0 | 32 |
AACTCGT | 35 | 0.0012522003 | 40.0 | 37 |
CCGTCTT | 1790 | 0.0 | 36.173187 | 47 |
GCCGTCT | 1805 | 0.0 | 36.06648 | 46 |
GTCTTCT | 1805 | 0.0 | 35.872574 | 49 |
CGTATGC | 1825 | 0.0 | 35.863014 | 41 |
TATGCCG | 1825 | 0.0 | 35.863014 | 43 |
TCGTATG | 1825 | 0.0 | 35.863014 | 40 |
TCTTCTG | 1800 | 0.0 | 35.77778 | 50 |
TCTCGTA | 1765 | 0.0 | 35.69405 | 38 |
CTTGAAA | 1805 | 0.0 | 35.67867 | 57 |
CTCGTAT | 1815 | 0.0 | 35.67493 | 39 |
CGTCTTC | 1815 | 0.0 | 35.67493 | 48 |