FastQCFastQC Report
Wed 25 May 2016
SRR1780313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479215
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG407.024716E-427.50078411
TCTATCG407.0677244E-427.47208431
TACCCTG450.00140527324.41963232
ACTGAGT450.001406127724.4170845
CTATGCT551.6037974E-423.9731379
GTTAGAG809.051656E-519.2284553
TCGGTGT708.1691385E-418.83800331
CAAATAG600.00735999818.35302736
CGCCTAT750.001280076717.61890636
TAATACC750.001299043917.5803014
CTTAGGG750.001299043917.5803013
TGCCGAC4150.017.5274319
GCGACAA800.00196078116.53155517
TATCGCC800.001999518116.48325233
CTATGGG800.002000913516.4815313
GATTTAT1105.658834E-515.9837581
AGATCTC1552.6251837E-715.62967540
CTGCCGA4400.015.52964218
GACGAAA1004.946639E-415.39561423
GTAGATC1351.9313917E-514.68242338
Produced by FastQC (version 0.11.5)