FastQCFastQC Report
Wed 25 May 2016
SRR1780313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479215
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT200394.18163037467525No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG53931.125382135367215No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG31160.6502300637500913RNA PCR Primer, Index 34 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC25310.5281554208445062No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC7570.15796667466585979No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT5950.12416138893815928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACA207.774502E-444.088561
CTAGAGT250.002352283835.19724324
ATGCCGT25550.034.2709544
CGTCTTC4000.033.0008642
GCTTATA551.3692261E-732.0041733
TCTTCTG4300.030.69847544
CCGTCTT4350.030.3456241
TCAAGTG300.005743794629.33103823
GTCTTCT4550.029.01174543
CTTTATA1250.028.1636732
TATGCCG31950.027.47489543
TCTTTAT1250.026.4531361
GCCGTCT5000.026.40068640
CAGCTTA858.034476E-925.9344481
GCACTTA450.001380711624.4936451
TGTATAA450.001397621224.4425310
GTATGCC36250.024.27649542
CGTATGC36200.024.2492541
TTGGGAT35450.024.20120632
TCGTATG36350.024.14918540
Produced by FastQC (version 0.11.5)