FastQCFastQC Report
Wed 25 May 2016
SRR1780312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136200
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC200.00693895252.52
CGCCTTT200.00693895252.525
GCGTACA200.00693895252.52
TCCTGAA305.861067E-446.6666682
CGCGTAC400.002411978335.01
CTAGGTA400.002411978335.04
TATGACA450.00429526831.11110953
AGTTGCA450.00429526831.11110928
CTATGAA604.9615314E-429.16666813
AGCCGAT500.00718842228.0000026
CCCTAGT500.00718842228.00000227
TCTTACA500.00718842228.0000021
GCTTAGG500.00718842228.0000021
GCAGGCA500.00718842228.0000022
ATTCTAC500.00718842228.00000219
GGAAAAA657.9231703E-426.92307961
AAACTTC700.001221010225.021
TAGGAGT800.002655210721.8754
GAGACAG850.00377388720.5882347
CACACAT1055.4326595E-420.06
Produced by FastQC (version 0.11.5)