##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780312_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136200 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.281483113069015 34.0 32.0 34.0 12.0 34.0 2 30.25834067547724 34.0 31.0 34.0 12.0 34.0 3 30.08448604992658 34.0 31.0 34.0 11.0 34.0 4 29.918869309838474 34.0 31.0 34.0 11.0 34.0 5 29.9743906020558 34.0 31.0 34.0 11.0 34.0 6 32.888208516886934 37.0 33.0 38.0 11.0 38.0 7 33.275638766519826 38.0 34.0 38.0 12.0 38.0 8 33.18537444933921 38.0 34.0 38.0 11.0 38.0 9 33.45013950073422 38.0 34.0 38.0 12.0 38.0 10-11 33.215055066079294 38.0 34.0 38.0 11.0 38.0 12-13 33.289977973568284 38.0 34.0 38.0 11.0 38.0 14-15 33.20130690161527 38.0 34.0 38.0 11.0 38.0 16-17 33.25393538913363 38.0 34.0 38.0 11.0 38.0 18-19 33.262323788546254 38.0 34.0 38.0 11.0 38.0 20-21 33.28789280469897 38.0 34.0 38.0 11.0 38.0 22-23 33.29696035242291 38.0 34.0 38.0 11.0 38.0 24-25 33.31799926578561 38.0 34.0 38.0 11.0 38.0 26-27 33.27783406754772 38.0 34.0 38.0 11.0 38.0 28-29 33.37042217327459 38.0 34.0 38.0 11.0 38.0 30-31 33.383226872246695 38.0 34.0 38.0 11.0 38.0 32-33 33.44143906020558 38.0 34.0 38.0 11.0 38.0 34-35 33.39895374449339 38.0 34.0 38.0 11.0 38.0 36-37 33.3122577092511 38.0 34.0 38.0 11.0 38.0 38-39 33.33591042584435 38.0 34.0 38.0 11.0 38.0 40-41 33.399353891336276 38.0 34.0 38.0 11.0 38.0 42-43 33.45607562408223 38.0 34.0 38.0 14.5 38.0 44-45 33.53808370044053 38.0 34.0 38.0 16.0 38.0 46-47 33.44076358296623 38.0 34.0 38.0 11.0 38.0 48-49 33.4076321585903 38.0 34.0 38.0 11.0 38.0 50-51 33.346020558002934 38.0 34.0 38.0 11.0 38.0 52-53 33.41796255506608 38.0 34.0 38.0 11.0 38.0 54-55 33.365242290748895 38.0 34.0 38.0 11.0 38.0 56-57 33.319331864904555 38.0 34.0 38.0 11.0 38.0 58-59 33.38698604992658 38.0 34.0 38.0 11.0 38.0 60-61 33.42685022026431 38.0 34.0 38.0 15.0 38.0 62-63 33.28765418502202 38.0 34.0 38.0 11.0 38.0 64-65 33.22457415565345 38.0 34.0 38.0 11.0 38.0 66-67 33.11108663729809 38.0 33.5 38.0 11.0 38.0 68-69 33.03692731277533 38.0 33.0 38.0 11.0 38.0 70-71 32.97142070484581 38.0 33.0 38.0 11.0 38.0 72-73 32.89216226138032 38.0 33.0 38.0 11.0 38.0 74-75 32.89637298091043 38.0 33.0 38.0 11.0 38.0 76 31.25998531571219 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.374540007928136 1101 2 0.07698780735984556 1101 3 0.5832118792767993 1101 4 0.43764631113898034 1101 5 -0.2631764166345434 1101 6 0.17091953659907233 1101 7 -0.3066244680198835 1101 8 -0.9714553907709416 1101 9 -0.8597911063230583 1101 10-11 -0.5890494778229396 1101 12-13 -0.45097147039589913 1101 14-15 -0.20420347285161 1101 16-17 -0.511549449836366 1101 18-19 -0.5341639592301917 1101 20-21 -0.42395476810212074 1101 22-23 -0.3308491834305798 1101 24-25 -0.4534355807325099 1101 26-27 -0.7947344895176016 1101 28-29 -0.39876657169180163 1101 30-31 -0.08563968192580518 1101 32-33 0.10684093408264062 1101 34-35 -0.42123952835056855 1101 36-37 -0.39982244775802656 1101 38-39 0.026585637028979647 1101 40-41 -0.2513924549426605 1101 42-43 -0.0015174622437186258 1101 44-45 -0.03166290278748107 1101 46-47 -0.14308455675723764 1101 48-49 -0.4810735089182714 1101 50-51 -0.1506563386137998 1101 52-53 -0.25271713653848593 1101 54-55 0.20418436947103658 1101 56-57 -0.15655386461157406 1101 58-59 0.02034847057942102 1101 60-61 -0.30686255415178465 1101 62-63 -0.39337429046020134 1101 64-65 0.3010960388660351 1101 66-67 -0.1947861711210379 1101 68-69 -0.3148737446688443 1101 70-71 -0.13511528591022426 1101 72-73 -0.17645365226101006 1101 74-75 -0.3233167678632185 1101 76 -1.2530402963736904 1102 1 -0.14521312006466047 1102 2 -0.2644023632986183 1102 3 0.25068422967597925 1102 4 0.06878964959939182 1102 5 -0.18714916987558894 1102 6 -0.10616242340972093 1102 7 -0.37203133830480084 1102 8 0.2034512231600587 1102 9 -0.06765750931141667 1102 10-11 0.03359755945824361 1102 12-13 -0.028243864741462232 1102 14-15 0.2917314864979872 1102 16-17 0.13197212051447593 1102 18-19 -0.20592108220682093 1102 20-21 0.015948549849973404 1102 22-23 -0.030658749093795734 1102 24-25 -0.05407280332094899 1102 26-27 -0.2745191522290966 1102 28-29 -0.2394609245812731 1102 30-31 0.23344769813718358 1102 32-33 -0.07956498403059697 1102 34-35 -0.2241399897876164 1102 36-37 -0.04173265064384424 1102 38-39 0.00809153316902922 1102 40-41 0.32910267433296525 1102 42-43 0.10362426484922338 1102 44-45 0.2517180391300471 1102 46-47 -0.29543202617787756 1102 48-49 0.10861373930556084 1102 50-51 0.08932754771154805 1102 52-53 -0.08938307076540042 1102 54-55 -0.07553437366234306 1102 56-57 0.1618376976824294 1102 58-59 0.03950177022053225 1102 60-61 0.03709115565558818 1102 62-63 -0.09849404436044296 1102 64-65 -0.07010442989541588 1102 66-67 0.025385219782052104 1102 68-69 0.2563926436707078 1102 70-71 0.3414832638590539 1102 72-73 0.4225722028624048 1102 74-75 0.07256692070339454 1102 76 0.18964629265436272 1103 1 0.39212665873853325 1103 2 0.7490598794319183 1103 3 0.30633500239992273 1103 4 0.2742829477756139 1103 5 0.00799475453662879 1103 6 -0.5732246075450789 1103 7 0.10929144789603384 1103 8 -0.1465304658460198 1103 9 0.1636323171003653 1103 10-11 0.1813709425974821 1103 12-13 0.22770720828278002 1103 14-15 0.24444517579703984 1103 16-17 0.6025646642777502 1103 18-19 0.41148168641546334 1103 20-21 0.49601520186784853 1103 22-23 0.09557724299585146 1103 24-25 0.33305090575397855 1103 26-27 0.40781806303495216 1103 28-29 0.03847066554543943 1103 30-31 -0.06161565790178969 1103 32-33 0.2571412343829351 1103 34-35 -0.1003686574797058 1103 36-37 0.04116854323295627 1103 38-39 -0.33482577438243055 1103 40-41 0.37983877628857243 1103 42-43 0.44517923445297924 1103 44-45 0.17779655667197858 1103 46-47 0.4782367645640875 1103 48-49 0.1961036682589068 1103 50-51 -0.24450018245764227 1103 52-53 0.006892473071118843 1103 54-55 0.34787806316472825 1103 56-57 0.5009536428959365 1103 58-59 0.36794606817701947 1103 60-61 0.2722665749773512 1103 62-63 0.2455145984286844 1103 64-65 0.41310805087803715 1103 66-67 0.5777363514014837 1103 68-69 0.13257370277860048 1103 70-71 0.1961159453210044 1103 72-73 0.2769998011924386 1103 74-75 0.24980635525990635 1103 76 0.7046173612839652 1104 1 -0.10198173536986133 1104 2 -0.100832870460831 1104 3 -0.19556689950197992 1104 4 0.41347928697195613 1104 5 -0.5553257087838368 1104 6 -0.2498583841788502 1104 7 -0.18471684611226635 1104 8 -1.0410964604120139 1104 9 0.012223765691807387 1104 10-11 -0.4423670811405458 1104 12-13 -0.4401034595278901 1104 14-15 -0.1340976527457869 1104 16-17 -0.6694216077085287 1104 18-19 -0.17033584540207158 1104 20-21 -0.6318769760243299 1104 22-23 -0.4093205119019032 1104 24-25 -0.38708351438044275 1104 26-27 -0.3097495045326184 1104 28-29 -0.6719683448935783 1104 30-31 -0.36724271352883875 1104 32-33 -0.3421795149378113 1104 34-35 -0.22107507818611793 1104 36-37 -0.6963332442688284 1104 38-39 -0.48374612330277245 1104 40-41 -0.4474814010316024 1104 42-43 -0.17579888652514342 1104 44-45 -0.1653680364926089 1104 46-47 -0.7244873881600711 1104 48-49 -0.4460384738832346 1104 50-51 -0.586985524942989 1104 52-53 -0.5348921687135189 1104 54-55 -0.7145200492333856 1104 56-57 -0.16949537755308342 1104 58-59 -0.22579052555957446 1104 60-61 -0.11070210799133662 1104 62-63 -0.41582531291122393 1104 64-65 -0.3754018876318952 1104 66-67 -0.48961671595158407 1104 68-69 0.10211467231956561 1104 70-71 -0.30421295500789824 1104 72-73 -0.34794657375393 1104 74-75 -0.48244017698662844 1104 76 -0.5117990551324496 1105 1 0.016349541138357182 1105 2 0.2152869644257578 1105 3 -0.06469608230408497 1105 4 0.5812027361967438 1105 5 0.8257543371755673 1105 6 0.292625693157774 1105 7 0.36887408683524114 1105 8 0.0962852721439802 1105 9 0.5471117751106505 1105 10-11 0.11323457566754769 1105 12-13 -0.5126739235192659 1105 14-15 -0.13851982673900665 1105 16-17 0.2404894523365826 1105 18-19 0.00656175307729967 1105 20-21 0.33900121737397626 1105 22-23 0.005076554639934727 1105 24-25 -0.09309937321131656 1105 26-27 -0.10197470560500221 1105 28-29 -0.005308770727296519 1105 30-31 0.0037056956233243454 1105 32-33 -0.061266851987618054 1105 34-35 -0.07083818867309333 1105 36-37 0.021789512727920624 1105 38-39 0.23994779275039235 1105 40-41 -0.025885446593825634 1105 42-43 0.0808396938399838 1105 44-45 0.19567569586479294 1105 46-47 0.25558623199397346 1105 48-49 0.1621930820694999 1105 50-51 0.036874355619303856 1105 52-53 -0.1323112078215658 1105 54-55 0.2800967429705423 1105 56-57 0.26476423633119595 1105 58-59 0.344052872031817 1105 60-61 0.3160713717311978 1105 62-63 0.10963676348919194 1105 64-65 -0.3364463292393438 1105 66-67 0.1607194685669029 1105 68-69 0.15249979509074763 1105 70-71 0.2057094530378336 1105 72-73 0.47499270025772944 1105 74-75 -0.02980744084182163 1105 76 -0.1343290918716029 1106 1 0.5170803795826657 1106 2 0.27553155658852546 1106 3 0.9373605484939631 1106 4 0.6711729885012723 1106 5 0.4900754653844608 1106 6 0.7548020492487026 1106 7 0.6215091450452377 1106 8 0.9074865648833423 1106 9 0.9123179890737063 1106 10-11 0.5672835675512005 1106 12-13 0.7133791816259887 1106 14-15 0.5862074860525013 1106 16-17 0.9881996142962564 1106 18-19 0.6687197463932435 1106 20-21 0.9584180156405253 1106 22-23 0.5506309268166589 1106 24-25 0.680329691388927 1106 26-27 1.0190176187550506 1106 28-29 0.9294739976446564 1106 30-31 0.11617034728148212 1106 32-33 0.863379664593964 1106 34-35 0.8027598134569871 1106 36-37 0.4152707276091192 1106 38-39 0.9396466432406712 1106 40-41 0.8836521787321132 1106 42-43 0.6543054839901856 1106 44-45 0.7952900775901597 1106 46-47 0.7072260277999192 1106 48-49 0.6675586849193991 1106 50-51 0.49361533031540006 1106 52-53 0.4229791076509102 1106 54-55 0.4117247866004874 1106 56-57 0.4374771527071175 1106 58-59 0.4638920141229761 1106 60-61 0.6807634279947479 1106 62-63 0.5436078972069183 1106 64-65 0.7116641412149534 1106 66-67 0.5185915902019218 1106 68-69 0.7926995820825624 1106 70-71 0.7131096513558077 1106 72-73 0.9895507082638986 1106 74-75 0.3525275696249537 1106 76 0.9508594938274655 1107 1 -0.5473720578551564 1107 2 -0.5710820502072202 1107 3 -0.04959131497890823 1107 4 -0.5383548296666305 1107 5 -0.35261222115414625 1107 6 -0.20129345851895408 1107 7 -0.3580194384092721 1107 8 -0.27301393255294926 1107 9 -0.3200306755011795 1107 10-11 -0.10449648908000597 1107 12-13 -0.09589991839697376 1107 14-15 -0.8801811264828956 1107 16-17 -0.4987866870736042 1107 18-19 0.07388543094210576 1107 20-21 -0.23508852755990972 1107 22-23 0.062133184412125786 1107 24-25 -0.11159653498285138 1107 26-27 -0.4382411246443496 1107 28-29 -0.03186754976484707 1107 30-31 -0.6243515781796063 1107 32-33 -0.4675379140392266 1107 34-35 -0.2653392146290301 1107 36-37 -0.36968756427118876 1107 38-39 -0.7386306966230975 1107 40-41 -0.3664166970618723 1107 42-43 -0.30036659070179184 1107 44-45 -0.46096231393045883 1107 46-47 -0.5505492840543695 1107 48-49 -0.3134086373427891 1107 50-51 -0.6015136204766662 1107 52-53 -0.24955475628113533 1107 54-55 -0.6140500660818304 1107 56-57 -0.1864916235996077 1107 58-59 -0.438901281966416 1107 60-61 -0.11141151652197578 1107 62-63 -0.501050402326257 1107 64-65 -0.11249213030873051 1107 66-67 0.034968723438325355 1107 68-69 -0.4141573858519365 1107 70-71 -0.6273854890742854 1107 72-73 -0.44200971111315823 1107 74-75 -0.5248199470200277 1107 76 -0.7515052417660115 1108 1 -0.8378282962164221 1108 2 -0.5500392196671804 1108 3 -0.7085298624649425 1108 4 -0.12369002519735872 1108 5 -0.14223047068859884 1108 6 -0.21917055349101844 1108 7 -0.8076004439958595 1108 8 -0.25088482020036906 1108 9 -0.9654217369536937 1108 10-11 -0.26941734569081177 1108 12-13 -0.211069067993499 1108 14-15 -0.39448125062939 1108 16-17 -0.44462002040693704 1108 18-19 -0.15161891418514273 1108 20-21 -0.3593902035375578 1108 22-23 -0.008063898145287851 1108 24-25 -0.5405226678195945 1108 26-27 -0.24030506008817554 1108 28-29 -0.20338368880891977 1108 30-31 -0.1552718140579401 1108 32-33 -0.31493083768913266 1108 34-35 -0.07041370252474621 1108 36-37 -0.46134647178205057 1108 38-39 -0.46436781642446334 1108 40-41 -0.44992849097869936 1108 42-43 0.28174079601453883 1108 44-45 -0.15617491479948598 1108 46-47 -0.03659056276324435 1108 48-49 -6.681035128650592E-4 1108 50-51 0.21334516288769834 1108 52-53 -0.44520962903097683 1108 54-55 -0.26987719209052585 1108 56-57 -0.1097070177647197 1108 58-59 -0.283330208099251 1108 60-61 0.036718526929291784 1108 62-63 -0.29862954571545686 1108 64-65 0.4504579007278906 1108 66-67 0.4744705856357143 1108 68-69 -0.04828215307725259 1108 70-71 -0.19164681744175738 1108 72-73 0.04133914053178245 1108 74-75 0.08587992883347795 1108 76 -0.0488360921694877 1109 1 0.5764879430998171 1109 2 0.15822288859492772 1109 3 0.33149862756354764 1109 4 0.3463242198168892 1109 5 0.5887524352943103 1109 6 0.6563280220075569 1109 7 0.3869152255198074 1109 8 0.6162091968936423 1109 9 0.5921993456673889 1109 10-11 0.9761310873576292 1109 12-13 0.40157338214895333 1109 14-15 0.6504973818492417 1109 16-17 0.4321249938380731 1109 18-19 1.0037585786923486 1109 20-21 0.7730884289410795 1109 22-23 0.604008751427358 1109 24-25 0.34694171964478926 1109 26-27 0.7130425182593996 1109 28-29 0.20633853341330877 1109 30-31 0.9147068184206901 1109 32-33 0.5658383930800994 1109 34-35 0.9558683487573063 1109 36-37 0.9308543829187954 1109 38-39 0.4605965710399218 1109 40-41 0.39063418708398245 1109 42-43 0.7724103116840553 1109 44-45 0.5432196720950984 1109 46-47 0.632960719288036 1109 48-49 0.6137905859458215 1109 50-51 0.6984235104660499 1109 52-53 0.8787219949006442 1109 54-55 0.832081497368165 1109 56-57 0.6404354323777284 1109 58-59 0.7334076836386387 1109 60-61 0.9793467320575004 1109 62-63 0.5678946708087622 1109 64-65 0.7697955075654974 1109 66-67 0.8568423805075156 1109 68-69 0.33084890105379827 1109 70-71 0.46583181503687143 1109 72-73 0.4653419895346289 1109 74-75 0.2736648291183812 1109 76 0.4953234519900569 1110 1 -0.7671680930769966 1110 2 -0.7515165973218458 1110 3 -0.11420853282505306 1110 4 -0.5081083077458324 1110 5 -0.48805226200900265 1110 6 -0.22664977786775609 1110 7 -0.08869462682134355 1110 8 -0.7246767561806706 1110 9 -0.4571807397237748 1110 10-11 -0.4716911222374307 1110 12-13 0.060079747026506425 1110 14-15 -0.2125137221590947 1110 16-17 -0.5036343325029691 1110 18-19 -0.8818551407163824 1110 20-21 -0.2749026689115226 1110 22-23 -0.44522533243276285 1110 24-25 -0.3820123569547178 1110 26-27 -0.9468686181060235 1110 28-29 -0.35629612340417083 1110 30-31 -0.689301515504539 1110 32-33 -0.7337212347343822 1110 34-35 -0.853954792090768 1110 36-37 -0.49571473894118157 1110 38-39 -0.5929189090683593 1110 40-41 -0.39438447743606986 1110 42-43 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12-13 -0.2943701015852298 2116 14-15 0.20713373645071442 2116 16-17 0.6640040106008129 2116 18-19 0.4482195871068484 2116 20-21 0.15369119181127644 2116 22-23 0.759507161286237 2116 24-25 0.22675769393751466 2116 26-27 0.2890877047697131 2116 28-29 0.14755300369754565 2116 30-31 0.05419201023146769 2116 32-33 -0.06948510125502594 2116 34-35 -0.01740982655575607 2116 36-37 0.0837439674944278 2116 38-39 0.278397041456671 2116 40-41 -0.30551634627585145 2116 42-43 0.34088162288792745 2116 44-45 -0.004753726168999606 2116 46-47 0.48236938555716335 2116 48-49 0.3839572194845502 2116 50-51 0.2576604083657372 2116 52-53 0.14157977665377786 2116 54-55 0.1553812058422892 2116 56-57 0.24084207784250822 2116 58-59 0.2092408513322752 2116 60-61 0.1784497930791673 2116 62-63 0.3722037876178774 2116 64-65 -0.2931144506932952 2116 66-67 0.13367601199230705 2116 68-69 0.2660589321940563 2116 70-71 0.4422033117572042 2116 72-73 0.5665975384645989 2116 74-75 0.48255305544846294 2116 76 0.17661610421294327 2117 1 -0.45852722094760523 2117 2 -0.6030206468421575 2117 3 0.040883627271128375 2117 4 -0.21540556771935115 2117 5 -0.18037807577168508 2117 6 0.13654792158229156 2117 7 0.054642957763668676 2117 8 -0.13141593782826533 2117 9 -0.9215067809419608 2117 10-11 0.06165547933363058 2117 12-13 -0.13279845867450035 2117 14-15 -0.13968734381935377 2117 16-17 -0.502452376223161 2117 18-19 -0.06308437524737798 2117 20-21 -0.9107098516313954 2117 22-23 -0.15032114806383134 2117 24-25 -0.12475205850059723 2117 26-27 0.04948803212427322 2117 28-29 -0.5367373870819705 2117 30-31 0.19197077310400346 2117 32-33 -0.0818418836324426 2117 34-35 -0.1648174170897505 2117 36-37 -0.6644785995109572 2117 38-39 -0.24374627846422214 2117 40-41 -0.3780117428522658 2117 42-43 -0.44332437179256345 2117 44-45 -0.2561219659562184 2117 46-47 0.016272747761355788 2117 48-49 -0.16820638959953982 2117 50-51 -0.0808915591716044 2117 52-53 -0.33839959318869006 2117 54-55 -0.3865397857692443 2117 56-57 -0.7332977639361147 2117 58-59 -0.4991578392494631 2117 60-61 -0.4919919650876494 2117 62-63 -0.31236366106247715 2117 64-65 -0.7976155759746177 2117 66-67 -0.2689189549312445 2117 68-69 -0.7589615274663117 2117 70-71 -0.6687647711726186 2117 72-73 -1.663556590976853 2117 74-75 -0.678754316849151 2117 76 -1.1918794287612116 2118 1 0.18784607757933713 2118 2 -0.026147119474504876 2118 3 -0.06026396651118304 2118 4 -0.5836611234632549 2118 5 -0.17753875585237466 2118 6 -0.10703182516731857 2118 7 -0.40687790064325924 2118 8 0.08883900767374797 2118 9 0.021138956687927646 2118 10-11 -1.3437188871166263 2118 12-13 -0.7801040712048035 2118 14-15 -0.39495491793831405 2118 16-17 -0.4771103864955677 2118 18-19 -0.5154516052577165 2118 20-21 -0.6440722844624105 2118 22-23 -0.4591798262120861 2118 24-25 0.19037007463384725 2118 26-27 -0.3444315854574711 2118 28-29 -0.21257604590589807 2118 30-31 -0.584502706800393 2118 32-33 -0.5762988531033955 2118 34-35 -0.41863142892166394 2118 36-37 -0.42320421102418493 2118 38-39 -0.4681330701174673 2118 40-41 -0.4693488506447139 2118 42-43 -0.4588982534395356 2118 44-45 -0.7552998345168973 2118 46-47 -0.6709274285307458 2118 48-49 -0.9507483861076054 2118 50-51 -0.2650829039074196 2118 52-53 -0.5960445680162039 2118 54-55 -0.4721813025593775 2118 56-57 -0.5603492785225939 2118 58-59 -0.4072062517729833 2118 60-61 -0.6980036952929254 2118 62-63 -0.5320592181624733 2118 64-65 -0.4953593760864976 2118 66-67 -0.34458047409453485 2118 68-69 -0.6201993014394986 2118 70-71 -0.17419338134670426 2118 72-73 -0.7686961838099009 2118 74-75 -0.5121370910766032 2118 76 -0.2305958866460429 2119 1 0.2914962907612271 2119 2 0.03149625166420833 2119 3 -0.8290837983250015 2119 4 0.6844003538113874 2119 5 0.2522544661432775 2119 6 0.5939489800366786 2119 7 0.2742508679574911 2119 8 0.46824378362210695 2119 9 0.02269779657400761 2119 10-11 0.20974932097585963 2119 12-13 0.5934782271150212 2119 14-15 0.7258256816673381 2119 16-17 0.5354698726931559 2119 18-19 0.042141208911715466 2119 20-21 0.11582845535457409 2119 22-23 0.27432596256090136 2119 24-25 0.39015436489825106 2119 26-27 0.7485595333682582 2119 28-29 0.3415831074333866 2119 30-31 -0.10763252024518266 2119 32-33 0.2437183362661557 2119 34-35 -0.03807648192221791 2119 36-37 0.3693184132195597 2119 38-39 0.09078686449552009 2119 40-41 0.36968197225422017 2119 42-43 0.3535985867498752 2119 44-45 -0.06278732166699541 2119 46-47 0.16901763289597227 2119 48-49 0.5935900759493933 2119 50-51 0.3850207378796 2119 52-53 0.5821308309625408 2119 54-55 0.24887483252884834 2119 56-57 0.2980027328021748 2119 58-59 0.11053924444367169 2119 60-61 -0.18415529879146675 2119 62-63 0.3406804640027161 2119 64-65 0.1223035437470017 2119 66-67 -0.6848710081446541 2119 68-69 -0.08431227941350983 2119 70-71 0.08181447183585533 2119 72-73 0.17885938672549884 2119 74-75 0.34503525763166465 2119 76 0.49862098589983717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 19.0 13 250.0 14 1102.0 15 1982.0 16 2717.0 17 3172.0 18 3329.0 19 2794.0 20 2214.0 21 1683.0 22 1240.0 23 1084.0 24 947.0 25 1050.0 26 1147.0 27 1257.0 28 1480.0 29 1746.0 30 2300.0 31 2736.0 32 3515.0 33 4546.0 34 6394.0 35 9476.0 36 17842.0 37 60178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62922173274596 17.611600587371512 10.395741556534508 28.363436123348016 2 21.79588839941263 18.99265785609398 29.331130690161526 29.880323054331864 3 19.64684287812041 19.50367107195301 29.186490455212923 31.662995594713657 4 15.474302496328928 19.584434654919235 29.43979441997063 35.5014684287812 5 15.805433186490456 30.59030837004405 32.95594713656388 20.648311306901615 6 29.787077826725405 30.081497797356825 17.494860499265783 22.636563876651984 7 29.16299559471366 27.641703377386197 18.556534508076357 24.638766519823786 8 25.270925110132158 29.05359765051395 18.323054331864906 27.35242290748899 9 24.03157121879589 17.185022026431717 18.616005873715125 40.16740088105727 10-11 26.392437591776797 22.20117474302496 24.298091042584435 27.108296622613803 12-13 25.506607929515418 19.94970631424376 24.017254038179146 30.526431718061676 14-15 24.65381791483113 21.029735682819386 22.213289280469898 32.10315712187959 16-17 25.454478707782673 22.995594713656388 22.88179148311307 28.668135095447873 18-19 24.500367107195302 22.448604992657856 23.509544787077825 29.541483113069017 20-21 24.444199706314244 22.784508076358296 23.83847283406755 28.93281938325991 22-23 26.057268722466958 22.33149779735683 22.988619676945667 28.622613803230546 24-25 24.958883994126285 23.557268722466958 22.594713656387665 28.88913362701909 26-27 24.04662261380323 23.73201174743025 23.995227606461086 28.226138032305432 28-29 24.87922173274596 22.869309838472834 25.02900146842878 27.22246696035242 30-31 24.95704845814978 22.40308370044053 24.897944199706316 27.741923641703377 32-33 23.935022026431717 23.6428046989721 24.755139500734213 27.667033773861966 34-35 24.18612334801762 23.28230543318649 24.796255506607928 27.73531571218796 36-37 24.56093979441997 22.19383259911894 24.937224669603523 28.308002936857562 38-39 23.37481644640235 22.15381791483113 26.908590308370044 27.562775330396477 40-41 23.451541850220263 21.595447870778266 26.87848751835536 28.074522760646108 42-43 22.08627019089574 21.513215859030836 27.022393538913363 29.378120411160058 44-45 21.573421439060205 21.23898678414097 28.7143906020558 28.473201174743025 46-47 21.476872246696036 21.98201174743025 28.739353891336272 27.801762114537443 48-49 21.058737151248163 22.30212922173275 28.3226872246696 28.316446402349488 50-51 20.713289280469898 23.505139500734217 27.381791483113073 28.399779735682817 52-53 19.70337738619677 23.58920704845815 27.54185022026432 29.16556534508076 54-55 19.843979441997064 23.24706314243759 27.544787077826726 29.36417033773862 56-57 20.01027900146843 23.417033773861966 26.97136563876652 29.601321585903083 58-59 20.475770925110133 23.21035242290749 26.665932452276063 29.647944199706316 60-61 20.184654919236415 22.443465491923643 27.090308370044053 30.28157121879589 62-63 20.669970631424377 22.43281938325991 26.38582966226138 30.511380323054333 64-65 21.703744493392072 22.435756240822318 25.222099853157122 30.638399412628488 66-67 21.515051395007344 23.29955947136564 24.08113069016153 31.104258443465493 68-69 21.566813509544787 23.687958883994124 23.927679882525698 30.81754772393539 70-71 22.718795888399413 23.448604992657856 24.55726872246696 29.27533039647577 72-73 24.266152716593243 23.24632892804699 24.025697503671072 28.461820851688692 74-75 23.582966226138034 24.558737151248163 24.062775330396477 27.79552129221733 76 23.43979441997063 24.433920704845814 24.132892804698972 27.99339207048458 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.5 11 1.5 12 1.0 13 1.5 14 3.5 15 4.0 16 6.5 17 10.0 18 10.0 19 14.0 20 22.5 21 27.0 22 37.5 23 62.0 24 95.0 25 114.0 26 169.5 27 303.0 28 425.0 29 469.0 30 548.5 31 794.0 32 1116.0 33 1272.0 34 1508.0 35 2034.0 36 2576.5 37 2829.0 38 3166.0 39 3999.0 40 4856.5 41 5526.0 42 5834.0 43 6017.5 44 6499.0 45 6901.5 46 7006.0 47 7058.0 48 6880.0 49 6419.0 50 6188.0 51 5872.5 52 5266.5 53 4595.5 54 4215.0 55 3992.0 56 3582.0 57 3317.0 58 3239.0 59 3173.0 60 3190.0 61 3358.5 62 3444.0 63 3437.0 64 3460.0 65 3536.5 66 3549.5 67 3516.0 68 3459.5 69 3328.5 70 3002.5 71 2751.0 72 2560.0 73 2160.0 74 1724.5 75 1498.0 76 1185.0 77 685.0 78 352.5 79 207.0 80 150.5 81 60.5 82 23.0 83 19.0 84 14.5 85 9.0 86 5.0 87 2.0 88 5.0 89 7.0 90 6.0 91 5.5 92 5.0 93 6.5 94 6.5 95 4.0 96 3.0 97 6.0 98 10.0 99 21.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 136200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.14831130690162 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21595222767333 50.41556534508076 2 13.97429351566142 18.487518355359764 3 4.691766377339224 9.310572687224669 4 1.9324261327058407 5.113069016152717 5 0.9345794392523363 3.0910425844346547 6 0.6293426865274048 2.497797356828194 7 0.4095722245654539 1.89647577092511 8 0.26860834239793996 1.42143906020558 9 0.24307945035185471 1.447136563876652 >10 0.700379603525207 6.319383259911894 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0014684287812041115 0.0 2 0.0 0.0 0.0 0.0022026431718061676 0.0 3 0.0 0.0 0.0 0.0022026431718061676 0.0 4 0.0 0.0 0.0 0.008076358296622614 0.0 5 0.0 0.0 0.0 0.013950073421439061 0.0 6 0.0 0.0 0.0 0.015418502202643172 0.0 7 0.0 0.0 0.0 0.016152716593245228 0.0 8 0.0 0.0 0.0 0.016152716593245228 0.0 9 0.0 0.0 0.0 0.020558002936857563 0.0 10 0.0 0.0 0.0 0.022026431718061675 0.0 11 0.0 0.0 0.0 0.024229074889867842 0.0 12 0.0 0.0 0.0 0.024229074889867842 0.0 13 0.0 0.0 0.0 0.028634361233480177 0.0 14 0.0 0.0 0.0 0.03010279001468429 0.0 15 0.0 0.0 0.0 0.0315712187958884 0.0 16 0.0 0.0 0.0 0.03671071953010279 0.0 17 0.0 0.0 0.0 0.038913362701908955 0.0 18 0.0 0.0 0.0 0.041116005873715125 0.0 19 0.0 0.0 0.0 0.04698972099853157 0.0 20 0.0 0.0 0.0 0.0513950073421439 0.0 21 0.0 0.0 0.0 0.05800293685756241 0.0 22 0.0 0.0 0.0 0.06754772393538913 0.0 23 0.0 0.0 0.0 0.07342143906020558 0.0 24 0.0 0.0 0.0 0.08149779735682819 0.0 25 0.0 0.0 0.0 0.08737151248164464 0.0 26 0.0 0.0 0.0 0.09985315712187959 0.0 27 0.0 0.0 0.0 0.18061674008810572 0.0 28 0.0 0.0 0.0 0.28193832599118945 0.0 29 0.0 0.0 0.0 0.40969162995594716 0.0 30 0.0 0.0 0.0 0.55359765051395 0.0 31 0.0 0.0 0.0 0.6784140969162996 0.0 32 0.0 0.0 0.0 0.8443465491923642 0.0 33 0.0 0.0 0.0 1.013215859030837 0.0 34 0.0 0.0 0.0 1.2848751835535976 0.0 35 0.0 0.0 0.0 1.6402349486049927 0.0 36 0.0 0.0 0.0 2.179882525697504 0.0 37 0.0 0.0 0.0 2.9008810572687223 0.0 38 0.0 0.0 0.0 3.6872246696035242 0.0 39 0.0 0.0 0.0 4.513950073421439 0.0 40 0.0 0.0 0.0 5.469897209985316 0.0 41 0.0 0.0 0.0 6.53230543318649 0.0 42 0.0 0.0 0.0 7.795888399412629 0.0 43 0.0 0.0 0.0 9.065345080763583 0.0 44 0.0 0.0 0.0 10.226138032305434 0.0 45 0.0 0.0 0.0 11.312041116005874 0.0 46 0.0 0.0 0.0 12.370778267254039 0.0 47 0.0 0.0 0.0 13.319383259911895 0.0 48 0.0 0.0 0.0 14.226872246696034 0.0 49 0.0 0.0 0.0 15.088839941262849 0.0 50 0.0 0.0 0.0 15.882525697503672 0.0 51 0.0 0.0 0.0 16.73641703377386 0.0 52 0.0 0.0 0.0 17.739353891336272 0.0 53 0.0 0.0 0.0 18.798825256975036 0.0 54 0.0 0.0 0.0 19.787812041116005 0.0 55 0.0 0.0 0.0 20.707048458149778 0.0 56 0.0 0.0 0.0 21.56461086637298 0.0 57 0.0 0.0 0.0 22.37224669603524 0.0 58 0.0 0.0 0.0 23.080029368575623 0.0 59 0.0 0.0 0.0 23.802496328928047 0.0 60 0.0 0.0 0.0 24.505139500734213 0.0 61 0.0 0.0 0.0 25.190161527165934 0.0 62 0.0 0.0 0.0 25.93612334801762 0.0 63 0.0 0.0 0.0 26.75697503671072 0.0 64 0.0 0.0 0.0 27.634361233480178 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATAAC 20 0.006938952 52.5 2 CGCCTTT 20 0.006938952 52.5 25 GCGTACA 20 0.006938952 52.5 2 TCCTGAA 30 5.861067E-4 46.666668 2 CGCGTAC 40 0.0024119783 35.0 1 CTAGGTA 40 0.0024119783 35.0 4 TATGACA 45 0.004295268 31.111109 53 AGTTGCA 45 0.004295268 31.111109 28 CTATGAA 60 4.9615314E-4 29.166668 13 AGCCGAT 50 0.007188422 28.000002 6 CCCTAGT 50 0.007188422 28.000002 27 TCTTACA 50 0.007188422 28.000002 1 GCTTAGG 50 0.007188422 28.000002 1 GCAGGCA 50 0.007188422 28.000002 2 ATTCTAC 50 0.007188422 28.000002 19 GGAAAAA 65 7.9231703E-4 26.923079 61 AAACTTC 70 0.0012210102 25.0 21 TAGGAGT 80 0.0026552107 21.875 4 GAGACAG 85 0.003773887 20.588234 7 CACACAT 105 5.4326595E-4 20.0 6 >>END_MODULE