FastQCFastQC Report
Wed 25 May 2016
SRR1780312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136200
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT98977.266519823788546No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC25811.8950073421439058No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG24771.8186490455212923No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG19041.3979441997063142RNA PCR Primer, Index 34 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6350.46622613803230545No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3030.22246696035242292No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3020.22173274596182083RNA PCR Primer, Index 24 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC1800.13215859030837004No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT1710.12555066079295155No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1510.11086637298091041No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1370.10058737151248164No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAG150.002220684270.0000140
GGGCTAA150.002220684270.000011
AAGCGCA150.002220684270.0000148
AATTGCT150.002220684270.0000143
CACGTAA150.002220684270.0000161
TAGGAAC150.002220684270.0000124
TAAGCGC150.002220684270.0000147
TCGGATA207.903589E-570.037
TATACCG207.903589E-570.06
GCAAACC252.383535E-456.00000422
CGGATAC252.383535E-456.00000438
ATACAGT252.383535E-456.00000441
CAAGTGT200.00693895252.564
TTTCCGA200.00693895252.511
GCTTATC200.00693895252.545
TACAGTT200.00693895252.542
GTAAGCG200.00693895252.546
TGTCGGA200.00693895252.535
TAGTCTG200.00693895252.539
GTCGGAT200.00693895252.536
Produced by FastQC (version 0.11.5)