##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780312_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136200 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.75265051395007 34.0 34.0 34.0 34.0 34.0 2 33.73522026431718 34.0 34.0 34.0 34.0 34.0 3 33.742239353891335 34.0 34.0 34.0 34.0 34.0 4 33.76135095447871 34.0 34.0 34.0 34.0 34.0 5 33.74629221732746 34.0 34.0 34.0 34.0 34.0 6 37.51175477239354 38.0 38.0 38.0 37.0 38.0 7 37.50513215859031 38.0 38.0 38.0 37.0 38.0 8 37.414977973568284 38.0 38.0 38.0 37.0 38.0 9 37.435712187958885 38.0 38.0 38.0 37.0 38.0 10-11 37.39110499265786 38.0 38.0 38.0 37.0 38.0 12-13 37.37185756240822 38.0 38.0 38.0 37.0 38.0 14-15 37.29095080763583 38.0 38.0 38.0 36.0 38.0 16-17 37.324016152716595 38.0 38.0 38.0 36.0 38.0 18-19 37.28385462555066 38.0 38.0 38.0 36.0 38.0 20-21 37.31554331864905 38.0 38.0 38.0 36.5 38.0 22-23 37.30161160058737 38.0 38.0 38.0 36.0 38.0 24-25 37.30435022026432 38.0 38.0 38.0 36.0 38.0 26-27 37.187121879588844 38.0 38.0 38.0 36.0 38.0 28-29 37.16764684287812 38.0 38.0 38.0 36.0 38.0 30-31 37.14532305433187 38.0 38.0 38.0 36.0 38.0 32-33 37.10032305433187 38.0 38.0 38.0 36.0 38.0 34-35 37.16284508076358 38.0 38.0 38.0 36.0 38.0 36-37 37.1703046989721 38.0 38.0 38.0 36.0 38.0 38-39 36.45538913362702 38.0 37.0 38.0 34.0 38.0 40-41 36.767540381791484 38.0 37.0 38.0 34.5 38.0 42-43 37.03479809104258 38.0 38.0 38.0 36.0 38.0 44-45 37.10618942731277 38.0 38.0 38.0 36.0 38.0 46-47 37.056431718061674 38.0 38.0 38.0 36.0 38.0 48-49 37.08280837004405 38.0 38.0 38.0 36.0 38.0 50-51 36.93294419970631 38.0 38.0 38.0 35.0 38.0 52-53 36.92361967694567 38.0 38.0 38.0 35.0 38.0 54-55 36.909045521292214 38.0 38.0 38.0 35.5 38.0 56-57 36.959313509544785 38.0 38.0 38.0 36.0 38.0 58-59 36.84699706314244 38.0 37.5 38.0 35.0 38.0 60-61 36.84596182085169 38.0 38.0 38.0 35.0 38.0 62-63 36.83168135095448 38.0 37.5 38.0 35.0 38.0 64-65 36.771930983847284 38.0 37.5 38.0 34.5 38.0 66-67 36.48495961820852 38.0 37.0 38.0 34.0 38.0 68-69 36.38878487518355 38.0 37.0 38.0 34.0 38.0 70-71 35.661358296622616 38.0 37.0 38.0 30.5 38.0 72-73 34.41233113069016 38.0 36.5 38.0 22.5 38.0 74-75 34.03116740088106 38.0 36.0 38.0 21.0 38.0 76 33.34518355359765 38.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.017392158897358456 1101 2 0.038770916159556634 1101 3 0.048634733323915214 1101 4 0.08856058127400246 1101 5 -0.14318770792563384 1101 6 -0.12639517197328587 1101 7 0.02865318903734959 1101 8 0.2043156237073802 1101 9 0.2907365512425315 1101 10-11 0.0515432583602049 1101 12-13 0.03443482229973682 1101 14-15 0.054586189396765405 1101 16-17 0.1119228608872973 1101 18-19 0.17720197828288065 1101 20-21 0.12443826600265595 1101 22-23 0.21513284644528596 1101 24-25 0.07773216731765586 1101 26-27 -0.12449653011760375 1101 28-29 -0.07912193784372334 1101 30-31 0.2505722460025197 1101 32-33 -0.015549341569901287 1101 34-35 0.06996016515605419 1101 36-37 0.22191685378139425 1101 38-39 0.3825939094476425 1101 40-41 0.2931121875618956 1101 42-43 0.23518337000861322 1101 44-45 0.24224471297180372 1101 46-47 0.05148525257117598 1101 48-49 -0.12619622185220436 1101 50-51 0.2151203668510533 1101 52-53 0.36658173450689446 1101 54-55 0.2136962220610812 1101 56-57 -0.12811192342128663 1101 58-59 0.16233474087631805 1101 60-61 0.15114677921123132 1101 62-63 0.055153831083366356 1101 64-65 -0.0747326785855904 1101 66-67 0.05688216704523086 1101 68-69 -0.46118192168334815 1101 70-71 -0.13225807007041368 1101 72-73 -0.1989189980902779 1101 74-75 -0.17723879364894657 1101 76 -0.9022451447457698 1102 1 0.03453124920718409 1102 2 -0.005468445152970958 1102 3 -0.19526770570047347 1102 4 -0.1910116739080081 1102 5 -0.18339865057559734 1102 6 0.04660709858995915 1102 7 0.015981981244195254 1102 8 -2.548003752380623E-4 1102 9 -0.06435024286865598 1102 10-11 -0.0697487653708464 1102 12-13 0.050987960804100396 1102 14-15 0.09640849951176023 1102 16-17 0.060578833933512044 1102 18-19 0.05741145605333031 1102 20-21 -0.12067269862050267 1102 22-23 0.014847194940124098 1102 24-25 -0.042827416656557205 1102 26-27 0.0775469768039514 1102 28-29 0.05864998041599989 1102 30-31 0.016594365683175738 1102 32-33 0.0951589276749516 1102 34-35 0.10947529003703238 1102 36-37 0.05814332415420864 1102 38-39 0.39508200730160326 1102 40-41 0.31098371762855237 1102 42-43 0.18581693039826774 1102 44-45 0.10216560947807096 1102 46-47 -0.10379197587678135 1102 48-49 0.2038777545632371 1102 50-51 0.2501309872275286 1102 52-53 0.2554706233957873 1102 54-55 0.15997176589760187 1102 56-57 0.11765823698301858 1102 58-59 0.21426471963556537 1102 60-61 0.2809717261093425 1102 62-63 0.16337180515500194 1102 64-65 -0.035217553704612214 1102 66-67 0.20446877804648267 1102 68-69 0.20588758684957753 1102 70-71 0.23374207641754197 1102 72-73 0.2134812069928529 1102 74-75 0.17978896581784198 1102 76 0.3726394959925301 1103 1 0.11889366039886085 1103 2 0.08711926450790486 1103 3 0.08166776635695072 1103 4 0.10207409478751828 1103 5 0.0826381179001956 1103 6 0.10453575895764544 1103 7 -0.050325789941624066 1103 8 0.2148261342178941 1103 9 0.05079661130258728 1103 10-11 0.07526698208393157 1103 12-13 0.07137175923667627 1103 14-15 0.21824985306042777 1103 16-17 0.012223161187598919 1103 18-19 0.04702179810270479 1103 20-21 0.23419802576241722 1103 22-23 0.11663434794678551 1103 24-25 0.12127571086120525 1103 26-27 -0.05828031390139188 1103 28-29 -1.429588647496871E-4 1103 30-31 0.0254971709274443 1103 32-33 0.13099720497664435 1103 34-35 0.0046448498407372085 1103 36-37 0.10479973666427611 1103 38-39 0.2644257412794744 1103 40-41 -0.08796889351918935 1103 42-43 0.010859045684291857 1103 44-45 0.16371618444328107 1103 46-47 0.15974351082944338 1103 48-49 0.05563560997962469 1103 50-51 -0.016711464980780022 1103 52-53 0.021086239011410157 1103 54-55 -0.09020768184281991 1103 56-57 -0.058141953451318784 1103 58-59 0.012635041176622508 1103 60-61 0.21571134377579426 1103 62-63 0.17557425150378236 1103 64-65 0.15064269678978803 1103 66-67 0.029554839717903292 1103 68-69 0.05698624648481854 1103 70-71 0.16774192992959058 1103 72-73 0.23756748839620911 1103 74-75 -0.013875430285587242 1103 76 -0.19683973934036914 1104 1 -0.12968348817828712 1104 2 -0.0390211616325189 1104 3 -0.04131235662317323 1104 4 -0.048219055505633435 1104 5 -0.011699076436997302 1104 6 -0.12031766589578297 1104 7 -0.11842245803829599 1104 8 -0.3938539744622176 1104 9 -0.15049038998441233 1104 10-11 -0.18244072562377767 1104 12-13 -0.13848810062318506 1104 14-15 -0.25615312134254253 1104 16-17 -0.3680571190926827 1104 18-19 -0.17829637721547442 1104 20-21 -0.19548880392441248 1104 22-23 -0.19824481693237317 1104 24-25 -0.15006706048156815 1104 26-27 -0.17590508152616025 1104 28-29 -0.22516083388261876 1104 30-31 -0.25153700610673724 1104 32-33 -0.32457265059320406 1104 34-35 -0.19498334978746357 1104 36-37 -0.05067716881263351 1104 38-39 -0.40276287590913995 1104 40-41 -0.3953977509480424 1104 42-43 -0.2761136412883971 1104 44-45 -0.2854973147702182 1104 46-47 -0.6155031644172411 1104 48-49 -0.46714431280029345 1104 50-51 -0.9286662769355942 1104 52-53 -0.46675159882643413 1104 54-55 -0.6021910938262351 1104 56-57 -0.6731212184305804 1104 58-59 -0.24850466996308995 1104 60-61 -0.3336237055592548 1104 62-63 -0.35346907753954326 1104 64-65 -0.43359153744444967 1104 66-67 -0.9263868162237543 1104 68-69 -0.7989840094854301 1104 70-71 -0.7220264098387545 1104 72-73 -0.881208430379715 1104 74-75 -0.7035508199609737 1104 76 -0.9907622332628634 1105 1 0.034146178332349564 1105 2 0.026783056986715792 1105 3 0.18238510568018285 1105 4 -0.003968945571877214 1105 5 0.1456480447170918 1105 6 0.20675298959310595 1105 7 0.2408197792200255 1105 8 0.09175534224629445 1105 9 0.22095094794485703 1105 10-11 0.0673110368625629 1105 12-13 0.027309734745692538 1105 14-15 0.0932589103751198 1105 16-17 0.15155364448591513 1105 18-19 0.10331844327334494 1105 20-21 0.11715215952083469 1105 22-23 0.1305173998995457 1105 24-25 0.05334585070614395 1105 26-27 0.23833949625729645 1105 28-29 0.26370748675513056 1105 30-31 0.16586018810011183 1105 32-33 0.268190162008743 1105 34-35 0.23642153268980337 1105 36-37 0.3196754703067697 1105 38-39 0.06432792228085304 1105 40-41 0.1690269763453145 1105 42-43 0.06265963984413503 1105 44-45 -0.08926910647766562 1105 46-47 0.02230057191196977 1105 48-49 -0.15912056710899236 1105 50-51 -0.08479348802259068 1105 52-53 -0.02305186692294825 1105 54-55 -0.3903381071743226 1105 56-57 0.17614541702908326 1105 58-59 0.16892444049551614 1105 60-61 0.09843723320409481 1105 62-63 -0.06971232193291854 1105 64-65 0.0065335742624270665 1105 66-67 -0.3890235241687634 1105 68-69 0.07351765725216097 1105 70-71 -0.00400649409765208 1105 72-73 0.42701390532251793 1105 74-75 -0.05646654687134145 1105 76 0.08355298582447546 1106 1 -0.003431404391953663 1106 2 0.035545773208390585 1106 3 -0.07434852390591828 1106 4 0.1466131543950695 1106 5 -0.06760253371032121 1106 6 0.10486896887989161 1106 7 -0.02200294655028756 1106 8 0.029006067575906513 1106 9 -0.10658954197397463 1106 10-11 -0.12899070025594028 1106 12-13 0.057376942502138206 1106 14-15 0.07085999745139304 1106 16-17 0.15802101383476952 1106 18-19 0.15022431568878147 1106 20-21 0.03491998059396195 1106 22-23 -0.1266459186211506 1106 24-25 0.04245305258648813 1106 26-27 0.09131242952696539 1106 28-29 0.006632309554355231 1106 30-31 -0.0112855021566034 1106 32-33 0.3772393375201446 1106 34-35 0.12221241740829925 1106 36-37 0.10594745972981201 1106 38-39 -0.016229571567613732 1106 40-41 -0.03983857141626146 1106 42-43 0.05002972321113219 1106 44-45 -0.0022038451753800814 1106 46-47 0.19063947528704261 1106 48-49 0.13647603945019426 1106 50-51 0.24993051946942302 1106 52-53 0.27708401761192647 1106 54-55 0.3374117183793217 1106 56-57 0.15377955710032865 1106 58-59 0.19621519667458642 1106 60-61 0.17024670105087836 1106 62-63 0.23992078982292497 1106 64-65 0.4001984168934456 1106 66-67 0.2863115964746612 1106 68-69 0.49595329572584745 1106 70-71 0.3931399306700598 1106 72-73 0.22266560020664627 1106 74-75 0.9431404897440316 1106 76 0.339295025561519 1107 1 -0.10053862104459199 1107 2 -0.17539017230320297 1107 3 -0.21517656400693852 1107 4 0.044018832821642206 1107 5 0.021155406138156252 1107 6 -0.03934163799237922 1107 7 0.0184194916528142 1107 8 0.018238485116278014 1107 9 -0.029846601742860912 1107 10-11 -0.27705069872536825 1107 12-13 -0.17655969176740172 1107 14-15 0.0031283069332985747 1107 16-17 0.07540449894994339 1107 18-19 -0.22111952758052666 1107 20-21 -0.1341471350408412 1107 22-23 -0.1632916674540894 1107 24-25 0.034268312401287915 1107 26-27 -0.012349466853223134 1107 28-29 0.07481551916635709 1107 30-31 -0.09718892298769788 1107 32-33 0.06900868013895689 1107 34-35 -0.2573965211391851 1107 36-37 -0.13568795773402798 1107 38-39 0.07181665174301344 1107 40-41 -0.12690140144611206 1107 42-43 -0.04999706181985886 1107 44-45 -0.08082556400908203 1107 46-47 -0.393147928095523 1107 48-49 -0.06890009891362325 1107 50-51 -0.216213201354023 1107 52-53 0.09470091514004508 1107 54-55 6.82946549090957E-7 1107 56-57 -0.440982056123822 1107 58-59 0.12106022613811973 1107 60-61 -0.3416267205455057 1107 62-63 -0.28960014076557883 1107 64-65 -0.05742766407387023 1107 66-67 -0.22178896218454724 1107 68-69 -0.28425639280251147 1107 70-71 -0.2491612861356387 1107 72-73 -0.5170527743246112 1107 74-75 -0.3643057488723258 1107 76 -0.23411891796033046 1108 1 -0.07637660987141004 1108 2 -0.08412698173833633 1108 3 0.06807917776836092 1108 4 0.05072274343616101 1108 5 -0.0414609811989024 1108 6 -0.15034412092223448 1108 7 -0.029004468620307478 1108 8 0.1491354435272001 1108 9 0.10665246715844745 1108 10-11 -0.15558887377192576 1108 12-13 -0.23061773025280985 1108 14-15 0.06382042363100027 1108 16-17 -0.06713119316675886 1108 18-19 -0.015928652347753314 1108 20-21 0.0884397675041626 1108 22-23 -0.04196174814930487 1108 24-25 -0.058003568418079965 1108 26-27 -0.1410505841716656 1108 28-29 0.0038735576517723302 1108 30-31 0.002298972729242621 1108 32-33 -0.16967847069903286 1108 34-35 0.1111013062971935 1108 36-37 0.17657150843604796 1108 38-39 -0.24773642088268133 1108 40-41 0.022128704078404837 1108 42-43 0.06251069733594505 1108 44-45 0.1481005688276653 1108 46-47 0.1460047720906985 1108 48-49 0.05567314751716168 1108 50-51 -0.15379855206786175 1108 52-53 0.008248401173567288 1108 54-55 0.01718721305207538 1108 56-57 -0.029801112610478242 1108 58-59 -0.12760519906362333 1108 60-61 0.14296359602804642 1108 62-63 -0.027278601349067344 1108 64-65 0.1955751292222132 1108 66-67 0.14881159647466546 1108 68-69 -0.041775014776440855 1108 70-71 0.14841009809776295 1108 72-73 0.08790532623404346 1108 74-75 0.42812907421892277 1108 76 0.926433533932908 1109 1 0.08907923058443146 1109 2 -0.11553723814859751 1109 3 0.0856717703609533 1109 4 0.06838656109998453 1109 5 0.06502050028257855 1109 6 -0.13232417790229078 1109 7 0.19435735474151983 1109 8 0.04407846347022115 1109 9 0.0186182791242544 1109 10-11 0.03479574161269028 1109 12-13 0.0815973694622869 1109 14-15 0.01816515297573318 1109 16-17 0.15046139942583636 1109 18-19 0.10924941033031388 1109 20-21 0.21140985297424209 1109 22-23 0.01939865071108926 1109 24-25 0.1226232122087012 1109 26-27 0.023728618107540456 1109 28-29 0.1158037570819701 1109 30-31 0.21234170777197647 1109 32-33 0.007743181722624115 1109 34-35 0.09999019518608065 1109 36-37 0.14865128051582133 1109 38-39 0.049414576268311805 1109 40-41 0.0033608978106016707 1109 42-43 0.24057337539861834 1109 44-45 0.2063626770897713 1109 46-47 0.40148910257502735 1109 48-49 0.20898511332912761 1109 50-51 0.2748339265646109 1109 52-53 0.13153899946416914 1109 54-55 -0.12294811458325938 1109 56-57 0.09010629479693222 1109 58-59 0.23948881803039512 1109 60-61 0.18733681540126668 1109 62-63 -0.19209341616387832 1109 64-65 -0.04185364570655992 1109 66-67 0.11594122610429736 1109 68-69 0.09244863194721376 1109 70-71 0.28947135165901017 1109 72-73 -0.009922309093589377 1109 74-75 0.2404866240764747 1109 76 0.5285702860696588 1110 1 -0.16134890904038457 1110 2 -0.09981642464384777 1110 3 0.016009310116771758 1110 4 0.004185347314283661 1110 5 -0.08774445303639311 1110 6 -0.1208965986076791 1110 7 -0.11069114144271452 1110 8 -0.12114002584799266 1110 9 0.05245117445299741 1110 10-11 -0.12860364754847353 1110 12-13 -0.07875177149627888 1110 14-15 -0.06051783429451518 1110 16-17 -0.08309764438251221 1110 18-19 -0.06238525437913722 1110 20-21 0.02221554466635922 1110 22-23 -0.4028535610148083 1110 24-25 -0.08782812982158106 1110 26-27 -0.09960321575060505 1110 28-29 -0.014682151797288157 1110 30-31 -0.11596049172135281 1110 32-33 0.11709438248656312 1110 34-35 -0.01019755489086549 1110 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2115 60-61 0.3467964348015329 2115 62-63 0.14860336370203697 2115 64-65 0.23604248827265906 2115 66-67 -0.022590480676669245 2115 68-69 -0.32529380805042507 2115 70-71 -0.1714753288722406 2115 72-73 -0.015544228662093929 2115 74-75 -0.18523640550412068 2115 76 -0.26049360091707996 2116 1 -0.01300451684814874 2116 2 0.039774827047189376 2116 3 0.01213602531358049 2116 4 0.06467623180825655 2116 5 0.06706615058403997 2116 6 -0.03723042583180103 2116 7 0.031525247141971136 2116 8 -0.013332256731196424 2116 9 -0.16059559485705677 2116 10-11 0.03520569987148292 2116 12-13 -2.9874955657760438E-5 2116 14-15 0.061430242752443576 2116 16-17 -0.377531709962625 2116 18-19 0.06207522362124962 2116 20-21 0.0666697416643629 2116 22-23 0.1295821794992662 2116 24-25 0.050329764915254316 2116 26-27 0.0685424390841547 2116 28-29 0.01747174886623526 2116 30-31 0.18563230606257974 2116 32-33 0.06171687813817783 2116 34-35 -0.04552014073123445 2116 36-37 0.015783394740957135 2116 38-39 -0.0015154906501280152 2116 40-41 -0.1575870581954959 2116 42-43 -0.08493762307749364 2116 44-45 -0.026737932464321545 2116 46-47 0.18486136123798502 2116 48-49 0.08538890979107094 2116 50-51 0.24516785620087234 2116 52-53 -0.11064694766363914 2116 54-55 0.26944819496421957 2116 56-57 0.08030870651096933 2116 58-59 0.07203950377876112 2116 60-61 -5.441559099352844E-4 2116 62-63 0.3352536983925347 2116 64-65 -0.12157603356848057 2116 66-67 -0.047118636083474996 2116 68-69 0.235938163518135 2116 70-71 0.40089717820111304 2116 72-73 0.2286030006526545 2116 74-75 0.3568112447615519 2116 76 0.4996247484031926 2117 1 0.028682041154986848 2117 2 0.008582369841981574 2117 3 -0.04585858697586076 2117 4 -0.024937080610754947 2117 5 0.08305026024782336 2117 6 0.15763078947203013 2117 7 0.11553772753479308 2117 8 -0.30593949945096455 2117 9 -0.12570334906833835 2117 10-11 -0.045887016489423615 2117 12-13 0.02701097616621695 2117 14-15 -0.04956341475284631 2117 16-17 -0.0758881172462651 2117 18-19 0.01822335156231958 2117 20-21 -0.04284485289336715 2117 22-23 0.10424630007486968 2117 24-25 -0.12672302100849464 2117 26-27 0.1088394451538548 2117 28-29 -0.6727434185619785 2117 30-31 -0.16150845640076028 2117 32-33 -0.10069020090430314 2117 34-35 0.23423587781886113 2117 36-37 0.07468164203005045 2117 38-39 -0.45653407484162756 2117 40-41 -0.19591055952536607 2117 42-43 -0.21868839031476028 2117 44-45 -0.10066124283736855 2117 46-47 0.1638688972128861 2117 48-49 0.017040856868746346 2117 50-51 -0.1457992037459448 2117 52-53 -0.05912696539535034 2117 54-55 0.03345879988817302 2117 56-57 -0.1087641298799511 2117 58-59 0.05849776607160351 2117 60-61 -0.4430823472759613 2117 62-63 -0.06512480532430942 2117 64-65 -0.10123572121766955 2117 66-67 -0.025418608803931875 2117 68-69 -0.009309227875171189 2117 70-71 0.13186969405735738 2117 72-73 -0.5036269318304676 2117 74-75 -0.018559586582647114 2117 76 -0.47250748920166785 2118 1 -0.0635153381951099 2118 2 0.023660429950808748 2118 3 -0.07596385370241876 2118 4 -0.035404424193892226 2118 5 0.04500337332903115 2118 6 0.11604958492233663 2118 7 0.013898550005258414 2118 8 0.0016563170018315532 2118 9 0.10356961552067645 2118 10-11 0.14388126110283395 2118 12-13 0.09588355426870265 2118 14-15 -0.0029265256304000786 2118 16-17 -0.09980620922327432 2118 18-19 -0.07577932672154475 2118 20-21 -0.07283993705589609 2118 22-23 0.04170074382243172 2118 24-25 -0.032762720633876086 2118 26-27 -0.075949428102291 2118 28-29 0.1578636026100284 2118 30-31 -0.07809601763684526 2118 32-33 -0.18947615856030353 2118 34-35 -0.21535983692695737 2118 36-37 -0.1255158390270239 2118 38-39 -0.1818404671254612 2118 40-41 -0.15986390992286914 2118 42-43 -0.09628827504684523 2118 44-45 -0.18337020830785633 2118 46-47 -0.1260087153852183 2118 48-49 -0.26999319801912236 2118 50-51 -0.07984222579940337 2118 52-53 -0.20374581847961792 2118 54-55 -0.07838921597811321 2118 56-57 -0.09748736823025439 2118 58-59 -0.1921845182672115 2118 60-61 -0.13415586447291616 2118 62-63 0.08723649177874648 2118 64-65 0.22006934887539842 2118 66-67 0.252207550231887 2118 68-69 -0.013330891462373984 2118 70-71 -0.29613871371984146 2118 72-73 0.04303538403750906 2118 74-75 -0.2592745866673951 2118 76 -0.4040417390279174 2119 1 -0.025429088005516576 2119 2 -0.007491089286119745 2119 3 -2.8007069293067843E-4 2119 4 -0.06706151312360475 2119 5 0.016969533864262587 2119 6 -0.047485358369598885 2119 7 -0.16803913010394922 2119 8 0.06074056176497322 2119 9 -0.037273674726883144 2119 10-11 0.05546554799678205 2119 12-13 0.08278929922564515 2119 14-15 -0.2552352938941098 2119 16-17 -0.1283961753501046 2119 18-19 0.03976862656381286 2119 20-21 -0.04590992475369404 2119 22-23 0.012828501283799199 2119 24-25 -0.1278772321692898 2119 26-27 0.038650582009097434 2119 28-29 0.09097673225495129 2119 30-31 -0.17770588635724494 2119 32-33 -0.4551740975211871 2119 34-35 -0.02472635810190127 2119 36-37 -0.19210358064720623 2119 38-39 -0.16493758706344153 2119 40-41 0.07606861337546178 2119 42-43 -0.0952476440958705 2119 44-45 -0.06143943765112425 2119 46-47 0.007836647698084676 2119 48-49 0.2143425676661792 2119 50-51 0.32746157102899076 2119 52-53 0.14403557318522786 2119 54-55 0.06587930893804383 2119 56-57 0.04680643517054506 2119 58-59 0.1258609493004883 2119 60-61 0.13608798864223814 2119 62-63 -0.017058322761435818 2119 64-65 -0.2640094188827433 2119 66-67 -0.12739102743204 2119 68-69 -0.09168998076282975 2119 70-71 0.04692402747903657 2119 72-73 0.19829162361013886 2119 74-75 0.3825629906426329 2119 76 -1.2909457987525457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 15.0 23 16.0 24 31.0 25 55.0 26 128.0 27 260.0 28 398.0 29 658.0 30 1095.0 31 1731.0 32 2702.0 33 4434.0 34 7504.0 35 12854.0 36 19438.0 37 84877.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.97136563876652 14.694566813509544 11.138766519823788 35.19530102790014 2 21.134361233480174 17.866372980910423 34.6431718061674 26.356093979441997 3 19.226138032305435 17.03303964757709 32.754772393538914 30.986049926578563 4 15.011013215859032 22.518355359765053 31.284140969162994 31.186490455212923 5 15.614537444933921 28.842143906020556 38.43906020558003 17.104258443465493 6 29.35168869309838 37.09544787077827 16.341409691629956 17.21145374449339 7 28.275330396475773 24.48531571218796 20.07856093979442 27.16079295154185 8 24.3920704845815 36.69676945668135 15.323054331864904 23.588105726872246 9 22.623348017621144 15.879588839941263 15.848751835535976 45.64831130690162 10-11 26.079295154185022 26.041483113069013 23.371879588839942 24.507342143906023 12-13 25.138399412628488 18.522393538913363 25.822687224669604 30.516519823788546 14-15 22.24265785609398 17.866005873715125 20.320851688693097 39.5704845814978 16-17 28.483480176211458 24.858296622613803 18.782672540381792 27.875550660792953 18-19 29.95631424375918 23.019089574155654 19.56020558002937 27.4643906020558 20-21 24.77900146842878 21.01027900146843 19.504405286343612 34.70631424375918 22-23 24.12077826725404 24.984214390602055 19.518355359765053 31.376651982378856 24-25 29.094713656387665 25.033406754772393 20.143171806167402 25.72870778267254 26-27 28.879221732745965 27.43906020558003 20.458516886930983 23.223201174743025 28-29 29.145374449339208 23.02863436123348 20.250367107195302 27.575624082232014 30-31 29.224669603524227 20.229809104258443 25.09324522760646 25.452276064610867 32-33 24.797723935389136 25.097650513950075 26.264317180616743 23.840308370044053 34-35 26.68575624082232 26.25 23.655286343612335 23.408957415565347 36-37 25.939427312775333 24.911894273127754 20.863436123348016 28.285242290748897 38-39 21.955212922173274 23.245961820851686 26.557635829662264 28.241189427312776 40-41 22.426578560939795 19.072320117474302 28.12775330396476 30.373348017621144 42-43 27.100954478707784 18.669603524229075 27.275330396475773 26.95411160058737 44-45 21.973201174743025 23.712187958883995 30.23237885462555 24.082232011747433 46-47 25.29221732745962 20.05690161527166 27.37114537444934 27.279735682819386 48-49 24.87151248164464 18.952643171806166 24.247797356828194 31.928046989720997 50-51 22.27496328928047 19.82488986784141 28.370044052863435 29.530102790014684 52-53 18.626651982378856 20.05433186490455 35.15491923641703 26.164096916299563 54-55 19.03083700440529 20.640969162995596 30.921071953010284 29.40712187958884 56-57 23.116005873715125 21.245594713656388 25.53230543318649 30.106093979441994 58-59 19.732745961820854 21.440161527165934 31.46035242290749 27.36674008810573 60-61 23.454478707782673 25.126651982378856 25.94162995594714 25.477239353891335 62-63 19.39023494860499 33.25550660792951 21.47063142437592 25.883627019089573 64-65 18.766886930983848 35.21842878120411 20.28157121879589 25.73311306901615 66-67 18.70411160058737 34.97613803230543 19.79588839941263 26.523861967694568 68-69 18.575991189427313 35.051762114537446 19.712555066079297 26.659691629955944 70-71 19.31718061674009 33.680616740088105 20.315345080763585 26.68685756240822 72-73 19.76505139500734 31.238619676945667 21.030837004405285 27.965491923641707 74-75 20.322320117474302 30.43281938325991 21.47870778267254 27.766152716593247 76 20.878854625550662 30.376651982378856 21.24302496328928 27.501468428781205 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.5 14 3.5 15 4.0 16 1.5 17 1.0 18 8.0 19 16.5 20 23.0 21 28.0 22 30.0 23 53.0 24 87.5 25 101.0 26 142.0 27 213.0 28 303.0 29 363.0 30 437.0 31 612.0 32 895.0 33 1077.0 34 1229.5 35 1637.0 36 2040.0 37 2188.0 38 2522.5 39 3279.5 40 6334.5 41 10146.5 42 11326.0 43 10540.0 44 8849.0 45 7344.5 46 6745.0 47 6479.0 48 5948.0 49 5324.0 50 4965.0 51 4741.0 52 4255.0 53 3703.0 54 3413.0 55 3218.0 56 2855.0 57 2678.0 58 2669.0 59 2629.0 60 2720.0 61 2970.0 62 3089.0 63 3219.5 64 3305.0 65 3329.5 66 3506.0 67 3613.0 68 3486.5 69 3276.5 70 2980.0 71 2767.0 72 2648.5 73 2214.0 74 1664.5 75 1431.0 76 1136.5 77 658.0 78 328.0 79 182.0 80 121.5 81 36.0 82 9.0 83 7.0 84 4.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 136200.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.428046989721 #Duplication Level Percentage of deduplicated Percentage of total 1 61.90559025855934 28.741556534508078 2 21.88661342610896 20.323054331864903 3 7.823199177670594 10.89647577092511 4 3.1248517434964813 5.80323054331865 5 1.5450304420020557 3.586637298091042 6 0.9883766901241401 2.753303964757709 7 0.6199098600458607 2.0146842878120412 8 0.45860678421760104 1.7033773861967694 9 0.3858622598244643 1.6123348017621146 >10 1.2303313038665296 8.312775330396475 >50 0.014232624337787617 0.49486049926578557 >100 0.009488416225191745 0.9133627019089574 >500 0.0015814027041986243 0.46622613803230545 >1k 0.0047442081125958725 5.1116005873715125 >5k 0.0015814027041986243 7.266519823788546 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 9897 7.266519823788546 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 2581 1.8950073421439058 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 2477 1.8186490455212923 No Hit CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG 1904 1.3979441997063142 RNA PCR Primer, Index 34 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 635 0.46622613803230545 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT 303 0.22246696035242292 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 302 0.22173274596182083 RNA PCR Primer, Index 24 (95% over 23bp) TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 180 0.13215859030837004 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT 171 0.12555066079295155 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 151 0.11086637298091041 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 137 0.10058737151248164 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0014684287812041115 0.0 2 0.0 0.0 0.0 0.0022026431718061676 0.0 3 0.0 0.0 0.0 0.0022026431718061676 0.0 4 0.0 0.0 0.0 0.007342143906020558 0.0 5 0.0 0.0 0.0 0.013215859030837005 0.0 6 0.0 0.0 0.0 0.014684287812041116 0.0 7 0.0 0.0 0.0 0.015418502202643172 0.0 8 0.0 0.0 0.0 0.015418502202643172 0.0 9 0.0 0.0 0.0 0.019823788546255508 0.0 10 0.0 0.0 0.0 0.021292217327459617 0.0 11 0.0 0.0 0.0 0.024229074889867842 0.0 12 0.0 0.0 0.0 0.024229074889867842 0.0 13 0.0 0.0 0.0 0.028634361233480177 0.0 14 0.0 0.0 0.0 0.030837004405286344 0.0 15 0.0 0.0 0.0 0.032305433186490456 0.0 16 0.0 0.0 0.0 0.037444933920704845 0.0 17 0.0 0.0 0.0 0.04038179148311307 0.0 18 0.0 0.0 0.0 0.042584434654919234 0.0 19 0.0 0.0 0.0 0.04919236417033774 0.0 20 0.0 0.0 0.0 0.053597650513950074 0.0 21 0.0 0.0 0.0 0.05873715124816446 0.0 22 0.0 0.0 0.0 0.06681350954478708 0.0 23 0.0 0.0 0.0 0.07268722466960352 0.0 24 0.0 0.0 0.0 0.08223201174743025 0.0 25 0.0 0.0 0.0 0.0881057268722467 0.0 26 0.0 0.0 0.0 0.09985315712187959 0.0 27 0.0 0.0 0.0 0.18355359765051396 0.0 28 0.0 0.0 0.0 0.290014684287812 0.0 29 0.0 0.0 0.0 0.4265785609397944 0.0 30 0.0 0.0 0.0 0.5690161527165932 0.0 31 0.0 0.0 0.0 0.6989720998531571 0.0 32 0.0 0.0 0.0 0.869309838472834 0.0 33 0.0 0.0 0.0 1.038179148311307 0.0 34 0.0 0.0 0.0 1.315712187958884 0.0 35 0.0 0.0 0.0 1.6798825256975036 0.0 36 0.0 0.0 0.0 2.234948604992658 0.0 37 0.0 0.0 0.0 2.9640234948604993 0.0 38 0.0 0.0 0.0 3.7599118942731278 0.0 39 0.0 0.0 0.0 4.584434654919236 0.0 40 0.0 0.0 0.0 5.545521292217328 0.0 41 0.0 0.0 0.0 6.618208516886931 0.0 42 0.0 0.0 0.0 7.8928046989721 0.0 43 0.0 0.0 0.0 9.179148311306902 0.0 44 0.0 0.0 0.0 10.337004405286343 0.0 45 0.0 0.0 0.0 11.43612334801762 0.0 46 0.0 0.0 0.0 12.506607929515418 0.0 47 0.0 0.0 0.0 13.46769456681351 0.0 48 0.0 0.0 0.0 14.407488986784141 0.0 49 0.0 0.0 0.0 15.287812041116005 0.0 50 0.0 0.0 0.0 16.100587371512482 0.0 51 0.0 0.0 0.0 16.96769456681351 0.0 52 0.0 0.0 0.0 17.991923641703377 0.0 53 0.0 0.0 0.0 19.058002936857562 0.0 54 0.0 0.0 0.0 20.062408223201174 0.0 55 0.0 0.0 0.0 20.98825256975037 0.0 56 0.0 0.0 0.0 21.86049926578561 0.0 57 0.0 0.0 0.0 22.684287812041116 0.0 58 0.0 0.0 0.0 23.405286343612335 0.0 59 0.0 0.0 0.0 24.133627019089573 0.0 60 0.0 0.0 0.0 24.847283406754773 0.0 61 0.0 0.0 0.0 25.537444933920703 0.0 62 0.0 0.0 0.0 26.28928046989721 0.0 63 0.0 0.0 0.0 27.10352422907489 0.0 64 0.0 0.0 0.0 27.98972099853157 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACAG 15 0.0022206842 70.00001 40 GGGCTAA 15 0.0022206842 70.00001 1 AAGCGCA 15 0.0022206842 70.00001 48 AATTGCT 15 0.0022206842 70.00001 43 CACGTAA 15 0.0022206842 70.00001 61 TAGGAAC 15 0.0022206842 70.00001 24 TAAGCGC 15 0.0022206842 70.00001 47 TCGGATA 20 7.903589E-5 70.0 37 TATACCG 20 7.903589E-5 70.0 6 GCAAACC 25 2.383535E-4 56.000004 22 CGGATAC 25 2.383535E-4 56.000004 38 ATACAGT 25 2.383535E-4 56.000004 41 CAAGTGT 20 0.006938952 52.5 64 TTTCCGA 20 0.006938952 52.5 11 GCTTATC 20 0.006938952 52.5 45 TACAGTT 20 0.006938952 52.5 42 GTAAGCG 20 0.006938952 52.5 46 TGTCGGA 20 0.006938952 52.5 35 TAGTCTG 20 0.006938952 52.5 39 GTCGGAT 20 0.006938952 52.5 36 >>END_MODULE