FastQCFastQC Report
Wed 25 May 2016
SRR1780311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465366
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG353.2368E-431.3921155
AATCGTT300.005716698229.35924539
CGAGAAT300.005719707329.35608314
TAACCGG300.005743825329.33082222
CGTTTAT300.005743825329.33082222
TTACTCG406.963047E-427.54208619
ATACTGG407.073408E-427.4681026
TGCAGTT450.001399370324.43709629
TCAATCC602.8375012E-422.0360421
CCGTTTA500.002571306722.00995413
ATTGCTA602.8749375E-421.99338527
GACTAGA500.002595506621.9744821
GGACTAG550.004509726519.97681
GGTATAG901.0098593E-519.5328731
TATTGCT951.5977997E-518.52074626
CATTGCG600.00737236618.34755114
ATCCGTA600.007390699318.33965512
TATGCAG600.0074182718.32782230
TGCCATT600.007427479618.3238811
GACTAGG600.007455162718.3120672
Produced by FastQC (version 0.11.5)