FastQCFastQC Report
Wed 25 May 2016
SRR1780311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465366
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT188704.0548729387192015No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG51171.0995646437427744No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC44160.9489305192042392No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG36030.7742293162800893TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC13110.2817137478887585No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT5840.12549262301070555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT2000.037.469361
GATATTA250.002330030835.265281
GTCTTCT5050.034.41298743
TCTTCTG5200.033.420344
CGTCTTC5550.032.50180442
ATGCCGT24300.032.40883344
TTTATAC2650.032.3885883
CCGTCTT5450.031.88726241
ATGCTAT353.2123586E-431.4327166
GGTACAT300.00568966529.3877351
CTTTATA2550.029.343512
GCCGTCT6300.027.58501240
TATGCCG30600.025.80831543
TGCCGTC6900.025.18631439
TAAGCCG450.001397599524.44241540
GTACAAG551.5716831E-424.044511
CTATAAC551.5878242E-424.0083264
TTGTACT752.0535244E-623.4748084
TTAATAC602.8623777E-422.0076332
TCCAGTA602.8623777E-422.0076332
Produced by FastQC (version 0.11.5)