FastQCFastQC Report
Wed 25 May 2016
SRR1780310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284477
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAG200.00694668752.522
TCTAACG200.00694668752.511
CCATAGG200.00694668752.54
GTACGAT200.00694668752.520
CGTCAGA501.7072553E-435.0000042
GCAACGC400.002416384235.017
CAACGCG450.00430306531.11111318
TCAGGTC450.00430306531.1111133
CGACGAA703.4817123E-530.022
CCGGTCG604.97498E-429.1666669
CTTCCGA657.944542E-426.92307718
TACGCAT657.944542E-426.92307728
GTCGTGT657.944542E-426.92307712
ACGCATC700.001224286425.029
GTAGTAC700.001224286425.03
TAGTACG750.001829657623.3333344
CCAAGAC1003.923601E-421.0000023
CCTTTAC1205.6983386E-520.41666635
GATATAG1055.4526847E-420.02
ACAGACG900.005267300619.44444545
Produced by FastQC (version 0.11.5)