##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780310_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 284477 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2131314658127 34.0 33.0 34.0 21.0 34.0 2 31.2815552751189 34.0 34.0 34.0 23.0 34.0 3 31.128421629868143 34.0 33.0 34.0 23.0 34.0 4 30.98882510712641 34.0 33.0 34.0 21.0 34.0 5 31.06755554930627 34.0 33.0 34.0 21.0 34.0 6 34.33755980272571 38.0 35.0 38.0 24.0 38.0 7 34.57301644772688 38.0 36.0 38.0 24.0 38.0 8 34.672149242293756 38.0 36.0 38.0 26.0 38.0 9 34.83246448746296 38.0 36.0 38.0 26.0 38.0 10-11 34.611191765942415 38.0 36.0 38.0 24.5 38.0 12-13 34.63932936581868 38.0 36.0 38.0 25.0 38.0 14-15 34.60585917314933 38.0 36.0 38.0 24.5 38.0 16-17 34.67626381043107 38.0 36.0 38.0 25.0 38.0 18-19 34.68364753565315 38.0 36.0 38.0 25.0 38.0 20-21 34.70730674184556 38.0 36.0 38.0 25.5 38.0 22-23 34.70557900990238 38.0 36.0 38.0 25.5 38.0 24-25 34.75615076086994 38.0 36.0 38.0 25.5 38.0 26-27 34.759850532731996 38.0 36.0 38.0 25.5 38.0 28-29 34.87853851102198 38.0 36.5 38.0 26.0 38.0 30-31 34.88476748559637 38.0 36.5 38.0 26.0 38.0 32-33 34.94472663870893 38.0 37.0 38.0 26.0 38.0 34-35 34.89835733644548 38.0 36.5 38.0 26.0 38.0 36-37 34.830643602118975 38.0 36.0 38.0 26.0 38.0 38-39 34.86280613195443 38.0 36.0 38.0 26.0 38.0 40-41 34.9349701381834 38.0 37.0 38.0 26.0 38.0 42-43 34.95419313336403 38.0 37.0 38.0 26.0 38.0 44-45 35.02265736773096 38.0 37.0 38.0 26.5 38.0 46-47 34.969964179880975 38.0 37.0 38.0 26.5 38.0 48-49 34.91423208203123 38.0 37.0 38.0 26.0 38.0 50-51 34.87017755389715 38.0 36.5 38.0 26.0 38.0 52-53 34.912045613529386 38.0 36.5 38.0 26.0 38.0 54-55 34.88656024915898 38.0 36.5 38.0 26.0 38.0 56-57 34.830452022483364 38.0 36.5 38.0 26.0 38.0 58-59 34.91358176583695 38.0 37.0 38.0 26.0 38.0 60-61 34.90426115292273 38.0 37.0 38.0 26.0 38.0 62-63 34.81326609884103 38.0 36.0 38.0 25.5 38.0 64-65 34.77233308843948 38.0 36.0 38.0 25.0 38.0 66-67 34.635104068167195 38.0 36.0 38.0 25.0 38.0 68-69 34.583846848778634 38.0 36.0 38.0 24.5 38.0 70-71 34.57533297946759 38.0 36.0 38.0 24.0 38.0 72-73 34.597948164526485 38.0 36.0 38.0 24.0 38.0 74-75 34.61458044059801 38.0 36.0 38.0 24.0 38.0 76 33.18358601925639 37.0 33.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.18594192746298788 1101 2 0.08776618892485288 1101 3 0.2945337123513774 1101 4 0.01008457396372009 1101 5 -0.28470381295794667 1101 6 0.07938596476754611 1101 7 -0.5685131183959768 1101 8 -0.2453402158106428 1101 9 -0.4037383744176495 1101 10-11 -0.31209184640277954 1101 12-13 0.2053662812678425 1101 14-15 -0.07407372473074503 1101 16-17 -0.17436934651829006 1101 18-19 -0.44266046468780473 1101 20-21 -0.04697805015320711 1101 22-23 -0.005291041283491893 1101 24-25 -0.1147307086438758 1101 26-27 -0.2892414646999484 1101 28-29 -0.45118007333969956 1101 30-31 -0.39288123889807025 1101 32-33 -0.3040503784355124 1101 34-35 -0.42111020228723106 1101 36-37 -0.3110521673973352 1101 38-39 -0.26977019830844995 1101 40-41 -0.3819842205835329 1101 42-43 -0.32047494110087626 1101 44-45 -0.2764913498309127 1101 46-47 -0.12718471013686639 1101 48-49 -0.25591260444873143 1101 50-51 -0.1695810299884002 1101 52-53 -0.006595343144681465 1101 54-55 0.01380138324106639 1101 56-57 0.016318234522373132 1101 58-59 -0.27875182270165766 1101 60-61 -0.2199510033453862 1101 62-63 -0.41568298446021146 1101 64-65 -0.10004288629347258 1101 66-67 -0.18270701064493977 1101 68-69 -0.29076636668043676 1101 70-71 -0.23916064373916868 1101 72-73 0.004443605828534203 1101 74-75 -0.2847933890081791 1101 76 -0.8486177432373623 1102 1 0.2031310127367476 1102 2 0.3692888565068948 1102 3 0.3173090641998293 1102 4 0.37710861062141987 1102 5 -0.16071664953900822 1102 6 -0.09744033425773324 1102 7 -0.21174317898655914 1102 8 0.12675698456934015 1102 9 0.381212969511715 1102 10-11 0.1419483237936845 1102 12-13 0.3071967713410402 1102 14-15 0.059854205210896794 1102 16-17 0.23623360557603235 1102 18-19 -0.16683163506787224 1102 20-21 0.06675900749166175 1102 22-23 0.0031030289824158785 1102 24-25 -0.09382536246332052 1102 26-27 -0.24714338619563136 1102 28-29 -0.1932769544017532 1102 30-31 0.2817677109450685 1102 32-33 0.28654810320769997 1102 34-35 0.2734322550239554 1102 36-37 0.2122520547840807 1102 38-39 -0.08266385745251625 1102 40-41 -0.21023507675474917 1102 42-43 -0.09385100981808847 1102 44-45 -0.223626269642196 1102 46-47 -0.42002320710733443 1102 48-49 -0.2539847224047733 1102 50-51 -0.12059566273286038 1102 52-53 0.13051379174319067 1102 54-55 -0.1972937176234879 1102 56-57 0.09652491305702426 1102 58-59 0.01930153532558876 1102 60-61 -0.06891960640923855 1102 62-63 -0.15741065415773647 1102 64-65 0.2535678012778746 1102 66-67 0.09671414576497028 1102 68-69 0.17516440519084142 1102 70-71 -0.11585602241032689 1102 72-73 0.24536499773542175 1102 74-75 -0.06834971270077261 1102 76 -0.031463185372963665 1103 1 0.11250257099192851 1103 2 0.17983668030439404 1103 3 0.3297948336858987 1103 4 0.23912176017253373 1103 5 0.22875443887744495 1103 6 -0.09193910496382784 1103 7 0.18292876826690474 1103 8 0.11561175730756901 1103 9 0.13144751804750143 1103 10-11 0.014777591001013946 1103 12-13 0.441340919494138 1103 14-15 -0.032316879868105275 1103 16-17 0.2285342949447795 1103 18-19 0.24032700874096946 1103 20-21 0.10865697795153295 1103 22-23 0.03736565659350077 1103 24-25 0.10450164825731179 1103 26-27 0.3357310478285527 1103 28-29 0.05663311987504471 1103 30-31 0.08200003451450755 1103 32-33 0.315659006655288 1103 34-35 0.08949706829366022 1103 36-37 0.37546816947368455 1103 38-39 -0.25029653034167154 1103 40-41 0.3519110145356166 1103 42-43 0.03791057020468003 1103 44-45 0.28606531293928583 1103 46-47 0.3236300763867561 1103 48-49 0.44406694873036656 1103 50-51 -0.29087697264471046 1103 52-53 0.23681248605877414 1103 54-55 0.4804171257500016 1103 56-57 0.4613693322851873 1103 58-59 0.2954884621649896 1103 60-61 0.4818976978956968 1103 62-63 0.4856389218007635 1103 64-65 0.37957877036196663 1103 66-67 0.24762526948422448 1103 68-69 0.3715881426151455 1103 70-71 0.37225524074015226 1103 72-73 0.4881449819381629 1103 74-75 0.535341135641815 1103 76 0.4219997893328795 1104 1 0.4440720923204857 1104 2 0.28737675049506706 1104 3 0.5914649375246768 1104 4 0.6875750327221937 1104 5 -0.1692664736012972 1104 6 0.4931526132604205 1104 7 0.19456811846047373 1104 8 -0.0349110546610234 1104 9 0.1460591175988597 1104 10-11 0.31033007119204825 1104 12-13 0.3738022965338246 1104 14-15 0.09402542629347721 1104 16-17 0.1440160417518257 1104 18-19 0.2516362095079998 1104 20-21 0.10231901348102923 1104 22-23 0.298897369028829 1104 24-25 0.35772625840963457 1104 26-27 0.15265821596053541 1104 28-29 -0.01109907014630096 1104 30-31 0.4028446743350571 1104 32-33 0.3862467630864117 1104 34-35 0.43555698363068274 1104 36-37 0.0906360481957762 1104 38-39 0.098336741484367 1104 40-41 -0.19385040953905275 1104 42-43 0.025212028289267607 1104 44-45 0.24285010978432098 1104 46-47 0.3118233901824752 1104 48-49 0.30123164354566967 1104 50-51 0.0573447430468903 1104 52-53 0.016313372487374522 1104 54-55 0.21351709318732048 1104 56-57 0.15158967310793514 1104 58-59 0.22703717955707958 1104 60-61 0.4886906354115297 1104 62-63 0.21055932413079148 1104 64-65 0.3524242061592133 1104 66-67 0.370533117090865 1104 68-69 0.25052189564528504 1104 70-71 0.4418814934989399 1104 72-73 0.592063358145694 1104 74-75 0.1897977006405469 1104 76 0.6458483366753995 1105 1 -0.15555334833756262 1105 2 -0.07882477711950386 1105 3 -0.26977918703466486 1105 4 -0.023221089486973767 1105 5 0.36852729843160503 1105 6 0.48019726660942297 1105 7 0.24790243103391418 1105 8 -0.08707762340367964 1105 9 0.06582997541319457 1105 10-11 0.20417921925314886 1105 12-13 -0.8676195601054175 1105 14-15 0.08936913957197845 1105 16-17 -0.1598544047816688 1105 18-19 -0.06226287979868772 1105 20-21 -0.032470939515441444 1105 22-23 0.02812034200180591 1105 24-25 0.0332183265647501 1105 26-27 0.09063926766441455 1105 28-29 -0.07677915240247302 1105 30-31 -0.028214373843560736 1105 32-33 -0.02060179743062207 1105 34-35 0.2941608320443123 1105 36-37 0.008904996491899908 1105 38-39 0.36254796357602714 1105 40-41 0.06910860926235074 1105 42-43 0.06598391430572548 1105 44-45 -0.035998303846696444 1105 46-47 0.05332603413076953 1105 48-49 0.27885355893931774 1105 50-51 0.23979553623137662 1105 52-53 0.10514269093625472 1105 54-55 0.1219997606373795 1105 56-57 -0.07252166649253411 1105 58-59 0.09290821363463664 1105 60-61 0.16702862013538322 1105 62-63 0.16000850156910218 1105 64-65 -0.2865777033590007 1105 66-67 0.22471569640931932 1105 68-69 0.17982198377689684 1105 70-71 0.049615042265088505 1105 72-73 0.11506570832195706 1105 74-75 0.15156327055829166 1105 76 0.1561083249245172 1106 1 -0.16856410408534472 1106 2 -0.33677777051809343 1106 3 0.13891788785721104 1106 4 -0.07567670068661059 1106 5 -0.0958336166605882 1106 6 -0.07070758201338379 1106 7 -0.5286312589273834 1106 8 -0.580241463603997 1106 9 -0.23313997678347675 1106 10-11 -0.43736343383130105 1106 12-13 -0.04161796666130613 1106 14-15 -0.3849508012429581 1106 16-17 -0.13557572278757846 1106 18-19 -0.16370622716951289 1106 20-21 -0.06295267181682362 1106 22-23 -0.12289405590394864 1106 24-25 -0.22731689993194948 1106 26-27 -0.45679770592994373 1106 28-29 -0.1406236208750471 1106 30-31 -0.4171072147267836 1106 32-33 -0.2130421855480833 1106 34-35 -0.14964536531513062 1106 36-37 -0.10448918174621014 1106 38-39 0.014885489184948142 1106 40-41 0.02378437183918436 1106 42-43 -0.3871553128738867 1106 44-45 -0.2801936145683044 1106 46-47 0.014734809455148934 1106 48-49 -0.22845209778729014 1106 50-51 -0.374857747401677 1106 52-53 -0.0043283425260653985 1106 54-55 -0.2457230369952157 1106 56-57 -0.17671441632181484 1106 58-59 -0.19532733155089232 1106 60-61 -0.13439907733787493 1106 62-63 -0.17264429833983996 1106 64-65 -0.09347575138995978 1106 66-67 -0.3082237811686497 1106 68-69 -0.05548489648716526 1106 70-71 -0.062374201987125844 1106 72-73 0.00230108278132235 1106 74-75 -0.11669020087352777 1106 76 0.04858641506793049 1107 1 -0.23673586226554022 1107 2 -0.42004943394564975 1107 3 0.04948557374874696 1107 4 -0.37336252306344875 1107 5 -0.29901405473593456 1107 6 -0.8657980811592267 1107 7 -0.4168317887122228 1107 8 -0.5105688789317924 1107 9 -0.03200329091269083 1107 10-11 -0.3355882050762915 1107 12-13 -0.015672515133850595 1107 14-15 -0.4126590322734103 1107 16-17 0.0030077944027624426 1107 18-19 -0.07580586268307599 1107 20-21 -0.08130321508834726 1107 22-23 -0.12367126962302422 1107 24-25 -0.03832947367648387 1107 26-27 -0.11204420111968716 1107 28-29 0.05686301346968037 1107 30-31 -0.24084396146058396 1107 32-33 -0.14562602780222988 1107 34-35 0.15538810934027225 1107 36-37 -0.11061265757737004 1107 38-39 -0.3651080441202552 1107 40-41 0.001775996259077317 1107 42-43 -0.13797748222521022 1107 44-45 -0.3692861678407269 1107 46-47 -0.22291276605302102 1107 48-49 -0.20256802798848383 1107 50-51 -0.05702920818307433 1107 52-53 -0.029899451961910017 1107 54-55 -0.05033202673841686 1107 56-57 -0.19745884701060135 1107 58-59 -0.18223573272111793 1107 60-61 -0.17440872550817943 1107 62-63 0.04113788816378161 1107 64-65 -0.16402497089102042 1107 66-67 -0.10470876944560814 1107 68-69 -0.10644729282984855 1107 70-71 -0.20308619545818374 1107 72-73 -0.15622112184287573 1107 74-75 0.01703983709668222 1107 76 -0.40447298861290193 1108 1 0.032565572323399294 1108 2 -0.27493839483894433 1108 3 -0.289346601777428 1108 4 -0.039415569740107514 1108 5 -0.12197281876047228 1108 6 0.2900091733245276 1108 7 0.21393870198200204 1108 8 -0.1161815434786817 1108 9 0.004582465744853437 1108 10-11 0.08130515678978156 1108 12-13 0.016448176915559998 1108 14-15 0.2934371695731457 1108 16-17 0.029224026016407834 1108 18-19 0.11082066350378028 1108 20-21 0.08055715728522017 1108 22-23 0.11951185143576737 1108 24-25 -0.15687088590449605 1108 26-27 0.13523634665187956 1108 28-29 0.10975035070311634 1108 30-31 0.3972917494425019 1108 32-33 0.16080238339674935 1108 34-35 0.16437609924224716 1108 36-37 -0.002252055541383413 1108 38-39 0.3476902054604736 1108 40-41 0.4557197805971711 1108 42-43 0.3230502303727505 1108 44-45 0.0843140130406752 1108 46-47 0.47675199803560986 1108 48-49 0.42115770167927025 1108 50-51 0.1733934062653617 1108 52-53 -0.011906559034443376 1108 54-55 0.30001086458675985 1108 56-57 0.19198451538956363 1108 58-59 0.3248935939229298 1108 60-61 0.13979794217920727 1108 62-63 0.17262496119644055 1108 64-65 0.318263428130507 1108 66-67 0.3043751801782122 1108 68-69 0.40991214843176493 1108 70-71 0.35947455826180885 1108 72-73 0.01731286641407337 1108 74-75 0.5008762320020992 1108 76 0.20410214304241237 1109 1 0.46063704436930664 1109 2 -0.11380023293997965 1109 3 0.05366342633606891 1109 4 0.3423410891011507 1109 5 0.567539687398412 1109 6 0.5489794077968924 1109 7 0.5598180458071766 1109 8 0.4279849058155847 1109 9 0.5695116023416702 1109 10-11 0.6686997311746126 1109 12-13 0.34717122998340955 1109 14-15 0.4669112153083006 1109 16-17 0.49166207488173086 1109 18-19 0.7073213301124781 1109 20-21 0.5340467089184102 1109 22-23 0.5923004495286861 1109 24-25 0.5692018469013505 1109 26-27 0.7086339497588483 1109 28-29 0.3959446671393678 1109 30-31 0.6525213671569077 1109 32-33 0.43455394123230207 1109 34-35 0.6574219938021386 1109 36-37 0.2507893466555302 1109 38-39 0.6864288658668158 1109 40-41 0.44521163637782735 1109 42-43 0.35046994749880156 1109 44-45 0.6927592931612452 1109 46-47 0.6065196993949158 1109 48-49 0.5821056627261285 1109 50-51 0.8345170766708279 1109 52-53 0.5527671649895467 1109 54-55 0.7422224114087683 1109 56-57 0.7106568047557076 1109 58-59 0.5646970168136889 1109 60-61 0.6536681128890081 1109 62-63 0.693362288385373 1109 64-65 0.842082009123466 1109 66-67 0.9946213181952004 1109 68-69 0.729063743945197 1109 70-71 0.7734784129990828 1109 72-73 0.43624382893906954 1109 74-75 0.8109543411520264 1109 76 0.5187631870096041 1110 1 -0.12524523297293655 1110 2 -0.16288346988467595 1110 3 0.08926831859499273 1110 4 -0.13483622315520094 1110 5 -0.3437720548797891 1110 6 -0.4500888353595158 1110 7 -0.646833841268176 1110 8 -0.3595691789207436 1110 9 -0.1976884147940936 1110 10-11 -0.3411801043221061 1110 12-13 -0.19793597560284582 1110 14-15 -0.30768395240792756 1110 16-17 0.018661603648382652 1110 18-19 -0.373733482893563 1110 20-21 -0.5022061291915207 1110 22-23 -0.13028769252674266 1110 24-25 -0.15973991294099932 1110 26-27 -0.3460478606339876 1110 28-29 -0.18758412093414734 1110 30-31 -0.3468654940006175 1110 32-33 -0.13310017349159153 1110 34-35 0.07612085427422954 1110 36-37 -0.20351976099987468 1110 38-39 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-0.219210044637137 2115 74-75 -0.19656312109763263 2115 76 -0.41584394860178975 2116 1 -0.37182845389502006 2116 2 -0.16225082984117378 2116 3 -0.24867126166664022 2116 4 -0.9065159086128425 2116 5 -1.211247036841705 2116 6 -0.0843496921946425 2116 7 0.18026720337216062 2116 8 0.20481370947319277 2116 9 -0.533379770107743 2116 10-11 -0.0975457179829533 2116 12-13 0.0342690625124078 2116 14-15 -0.0036141424090772034 2116 16-17 -0.1347666779757759 2116 18-19 -0.21534080687845858 2116 20-21 -0.22526664304162836 2116 22-23 -0.11992755826066315 2116 24-25 -0.4337985340994095 2116 26-27 -0.12712826343224037 2116 28-29 -0.34591144157285214 2116 30-31 -0.2740883610625531 2116 32-33 -0.3513871306498402 2116 34-35 -0.06822041581600757 2116 36-37 -0.23239899472115866 2116 38-39 -0.16055699477156793 2116 40-41 -0.253178635402719 2116 42-43 -0.13495148167739046 2116 44-45 -0.09110214956163531 2116 46-47 0.02789595881409923 2116 48-49 -0.14911417471353872 2116 50-51 -0.08610776457884839 2116 52-53 -0.09632584152979007 2116 54-55 -0.13167691972322615 2116 56-57 2.808840038710514E-4 2116 58-59 -0.008915505411358993 2116 60-61 -0.22346443304275 2116 62-63 -0.05928355023963405 2116 64-65 -0.41964963933983057 2116 66-67 -0.07891712054834699 2116 68-69 -0.19246597411423494 2116 70-71 -0.022167594959597636 2116 72-73 -0.13643206641272343 2116 74-75 -0.2557102088437375 2116 76 0.13858792665908481 2117 1 0.05293095117934499 2117 2 -0.09476968165136412 2117 3 0.19794999400973268 2117 4 0.33914088428804234 2117 5 -0.03205068638551012 2117 6 0.040957561065368964 2117 7 0.34666307055542944 2117 8 -0.1240930938430651 2117 9 -0.22802210648236354 2117 10-11 0.23758947345145032 2117 12-13 -0.47904698131544876 2117 14-15 0.07842768569574332 2117 16-17 -0.12437735164157004 2117 18-19 0.030232663104463597 2117 20-21 -0.18032434416232235 2117 22-23 -0.07786510773364341 2117 24-25 -0.10741745673408332 2117 26-27 0.22882724212956163 2117 28-29 -0.20506446411170032 2117 30-31 0.17075186724224523 2117 32-33 -0.17557577373612077 2117 34-35 0.02321607128976666 2117 36-37 -0.25114144358171586 2117 38-39 0.20635547450309843 2117 40-41 0.27023510454645816 2117 42-43 0.1845606626378995 2117 44-45 0.031778323712529755 2117 46-47 -0.04331388995480978 2117 48-49 -0.0018357095216927632 2117 50-51 0.20333001403215434 2117 52-53 0.03679814297214534 2117 54-55 -0.0841984795282471 2117 56-57 -0.3639079673897072 2117 58-59 -0.09587859335869808 2117 60-61 0.12093001765090605 2117 62-63 0.031090569662040934 2117 64-65 -0.07453132079631786 2117 66-67 -0.0722096256518796 2117 68-69 -0.3584191882957981 2117 70-71 0.09906725068469768 2117 72-73 -1.0696018785195491 2117 74-75 -0.17387668803383605 2117 76 -0.34780960698254404 2118 1 0.44094951804908433 2118 2 0.2480922963281067 2118 3 0.3834909060519536 2118 4 0.34481869936533727 2118 5 0.13385962900261106 2118 6 0.4245865275925098 2118 7 0.05860029685296553 2118 8 0.0226148785829281 2118 9 0.02767297688895809 2118 10-11 -0.45099411199898043 2118 12-13 0.07153357872986277 2118 14-15 0.14432004205806948 2118 16-17 0.019624679109810472 2118 18-19 0.19118352444696285 2118 20-21 0.3224040462945297 2118 22-23 0.05822804435167228 2118 24-25 0.24179407296047373 2118 26-27 0.044236086259033414 2118 28-29 0.05967575167290562 2118 30-31 0.11751371674560573 2118 32-33 0.2157030470466097 2118 34-35 -0.03612814797612174 2118 36-37 0.09063781236203283 2118 38-39 -0.23160645236222166 2118 40-41 -0.0845109133849391 2118 42-43 0.016657586118633105 2118 44-45 0.04606265653472974 2118 46-47 -0.1497936446068806 2118 48-49 -0.07565248601471097 2118 50-51 0.0030437057171255333 2118 52-53 -0.04792874168946781 2118 54-55 -0.16465664190547358 2118 56-57 -0.039469532311585453 2118 58-59 -0.16536102805000752 2118 60-61 -0.09214346239384952 2118 62-63 -0.03149663522281543 2118 64-65 -0.00856125711084843 2118 66-67 -0.04416591614602794 2118 68-69 0.01615946532985646 2118 70-71 0.08627144480794868 2118 72-73 0.15205912414668177 2118 74-75 -0.03649014072562551 2118 76 0.33633948056080243 2119 1 0.07241523337549083 2119 2 -0.07339353739386567 2119 3 -0.554674813699819 2119 4 0.05880728192054363 2119 5 0.32778768616472576 2119 6 -0.040599456932326916 2119 7 -0.04788933247155569 2119 8 -0.05262485712059117 2119 9 0.08912088862673073 2119 10-11 0.22605032208178955 2119 12-13 0.04966120629104154 2119 14-15 0.007007737157145755 2119 16-17 -0.028893504039075424 2119 18-19 -0.22854543889398116 2119 20-21 -0.06617970035666332 2119 22-23 -0.43826655499949396 2119 24-25 0.14916094603076147 2119 26-27 0.14090480674950356 2119 28-29 0.11575705586236751 2119 30-31 -0.21563465897479261 2119 32-33 -0.05185799747597031 2119 34-35 -0.4492786342557835 2119 36-37 0.0515745943038155 2119 38-39 -0.33090230851739477 2119 40-41 -0.13649109455262476 2119 42-43 -0.15673677187987067 2119 44-45 -0.1952934118337737 2119 46-47 -0.4103541002153577 2119 48-49 -0.5298644133978527 2119 50-51 -0.1799962485313955 2119 52-53 -0.268796215588317 2119 54-55 -0.29084000184773373 2119 56-57 -0.5985600855164037 2119 58-59 -0.41412618067408147 2119 60-61 -0.36575279106580183 2119 62-63 -0.1870788942701367 2119 64-65 -0.41574856243728675 2119 66-67 -0.5247785570343666 2119 68-69 -0.9129563805448555 2119 70-71 -0.7373496281050649 2119 72-73 -0.2916076662032907 2119 74-75 -0.35084943280319436 2119 76 -0.4245188362341423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 40.0 13 408.0 14 1315.0 15 2084.0 16 2610.0 17 3106.0 18 3341.0 19 2996.0 20 2447.0 21 1993.0 22 1710.0 23 1606.0 24 1733.0 25 1779.0 26 2199.0 27 2581.0 28 3174.0 29 3737.0 30 4718.0 31 5831.0 32 7388.0 33 9703.0 34 13264.0 35 20101.0 36 37939.0 37 146673.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.47665364862537 20.932447965916403 13.113889699343003 26.477008686115223 2 19.543935010563242 21.44742808733219 34.257954070100574 24.750682832003992 3 18.9498623790324 23.18746330986336 29.425577463204405 28.437096847899827 4 14.621920225536686 19.528116508540233 34.154958045817416 31.695005220105664 5 14.844785342927548 34.200655940550554 33.53557581104975 17.41898290547215 6 29.215015625164774 34.48257679882732 18.692899601725273 17.609507974282632 7 26.601096046429063 28.588602945053555 23.228942937390368 21.581358071127017 8 23.768178095241442 34.179213082252694 20.961975836359354 21.090632986146506 9 23.64479377946196 18.207447350752435 22.161369811970737 35.98638905781487 10-11 25.498370694291626 24.92679548786018 26.094200937158362 23.48063288068983 12-13 24.94437160121908 22.891481560899475 25.509619406841328 26.65452743104012 14-15 23.461340391912863 23.65924777617835 23.7093397872935 29.170072044615285 16-17 23.810536528436394 25.641088734765905 24.534672398823105 26.0137023379746 18-19 22.84982617223888 25.201510139659796 26.084885597078145 25.863778091023175 20-21 22.99693824105288 25.853056661874245 26.036375524207582 25.113629572865293 22-23 23.678188394843872 25.54582620036066 25.684853257029566 25.091132147765897 24-25 23.48713604263262 25.81491649588543 25.152297022254878 25.545650439227074 26-27 23.09958274306886 25.88803312745846 25.598378779303776 25.414005350168907 28-29 23.311902192444382 25.43263603032934 26.55504662942874 24.700415147797536 30-31 23.327234411278262 25.35552145783571 26.36650127514927 24.950742855736767 32-33 22.553317139874228 26.203700123384316 26.43271688045079 24.810265856290666 34-35 22.937179455632617 25.53861999388351 26.670873216463896 24.853327334019973 36-37 23.136141058855372 25.242989767186803 26.50548198975664 25.115387184201182 38-39 22.47492767429353 25.20643145140029 27.707160860104686 24.6114800142015 40-41 22.500764560931113 24.725021706499998 27.8635882689989 24.910625463569993 42-43 21.599109945619503 24.620443832014537 28.22038337018458 25.56006285218137 44-45 21.182907581280734 24.270151892771647 29.121510702095428 25.42542982385219 46-47 21.211556646055744 24.732052151843558 29.0403090583773 25.016082143723395 48-49 20.673551816139792 25.146496904846437 29.143656604927635 25.03629467408613 50-51 20.51642136271122 25.72562984002222 28.35027084790686 25.4076779493597 52-53 19.77980645183969 25.601542479708378 28.77350365758919 25.84514741086274 54-55 20.034132812142985 25.613494236792427 28.38050176288417 25.97187118818042 56-57 20.03852684048271 25.74426052018265 28.17661884792092 26.04059379141372 58-59 20.138359164361265 25.48958263761218 28.193843439012646 26.178214759013912 60-61 20.471778034779614 25.1247025242814 28.05973769408423 26.343781746854756 62-63 20.595513872826274 25.148430277315914 27.688530179944248 26.56752566991356 64-65 20.988164245264116 25.14438777124337 26.852082945194162 27.01536503829835 66-67 21.124554884929186 25.224007564759187 26.306696147667473 27.34474140264415 68-69 21.40876063794261 25.535280532345322 26.270665115281727 26.785293714430342 70-71 22.133775313997265 25.25933555261058 26.302829402728516 26.30405973066364 72-73 22.73597291867694 25.198390728232383 26.09108309473718 25.9745532583535 74-75 22.680216678325486 25.9544708359551 26.088576580883515 25.27673590483589 76 22.547692783599377 25.800328321797544 26.227779398686007 25.424199495917073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.5 7 2.0 8 1.0 9 5.0 10 6.5 11 6.0 12 8.0 13 18.0 14 27.0 15 46.0 16 87.5 17 109.0 18 142.0 19 243.5 20 409.0 21 506.0 22 603.0 23 832.0 24 1141.0 25 1318.0 26 1636.5 27 2166.0 28 2723.0 29 3069.0 30 3340.5 31 4157.0 32 5142.0 33 5582.0 34 6228.5 35 7431.5 36 8267.0 37 8546.0 38 9453.0 39 11195.0 40 12608.0 41 13517.0 42 13848.0 43 13904.5 44 14354.5 45 14756.0 46 14764.0 47 14674.5 48 13983.0 49 13047.5 50 12714.0 51 12004.5 52 10442.5 53 9117.5 54 8645.0 55 8280.0 56 7354.5 57 6453.0 58 6112.0 59 5746.5 60 5222.0 61 5021.5 62 4980.0 63 4840.0 64 4639.0 65 4405.0 66 4070.5 67 3909.0 68 3807.0 69 3520.0 70 3193.0 71 3051.0 72 2821.5 73 2350.5 74 1849.0 75 1589.0 76 1321.0 77 834.0 78 485.0 79 355.0 80 237.0 81 81.0 82 31.0 83 19.0 84 14.0 85 9.0 86 9.5 87 10.0 88 10.5 89 8.5 90 7.5 91 10.0 92 11.0 93 7.0 94 5.5 95 7.0 96 6.0 97 7.5 98 11.0 99 46.5 100 80.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.757611335890072E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.757611335890072E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.757611335890072E-4 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 284477.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.84638428327665 #Duplication Level Percentage of deduplicated Percentage of total 1 75.40306701676876 48.8961625990705 2 15.321362694279966 19.87069946033475 3 4.6487592121448 9.043656789334921 4 1.5568325037007718 4.038198351987039 5 0.7766124488315083 2.5180254648052256 6 0.48673889891240824 1.8937954610695782 7 0.3666670527367518 1.664392282404871 8 0.24350649971596133 1.2632412844845462 9 0.23239767512990347 1.3563134053212424 >10 0.9618922513212832 9.350281550463754 >50 0.0021637464578618693 0.10523335072356707 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002460655870246101 0.0 2 0.0 0.0 0.0 0.00949110121380639 0.0 3 0.0 0.0 0.0 0.010897190282518445 0.0 4 0.0 0.0 0.0 0.07381967610738302 0.0 5 0.0 0.0 0.0 0.08260773278683338 0.0 6 0.0 0.0 0.0 0.08506838865707948 0.0 7 0.0 0.0 0.0 0.08612295545861352 0.0 8 0.0 0.0 0.0 0.08647447772579155 0.0 9 0.0 0.0 0.0 0.09245035626781778 0.0 10 0.0 0.0 0.0 0.09596557893959792 0.0 11 0.0 0.0 0.0 0.09737166800830999 0.0 12 0.0 0.0 0.0 0.10229297974880219 0.0 13 0.0 0.0 0.0 0.10405059108469226 0.0 14 0.0 0.0 0.0 0.10791733602365042 0.0 15 0.0 0.0 0.0 0.10862038055800645 0.0 16 0.0 0.0 0.0 0.10897190282518446 0.0 17 0.0 0.0 0.0 0.11037799189389652 0.0 18 0.0 0.0 0.0 0.11283864776414262 0.0 19 0.0 0.0 0.0 0.11775995950463482 0.0 20 0.0 0.0 0.0 0.12022061537488092 0.0 21 0.0 0.0 0.0 0.12162670444359298 0.0 22 0.0 0.0 0.0 0.12619649391690715 0.0 23 0.0 0.0 0.0 0.13041476112304334 0.0 24 0.0 0.0 0.0 0.13674216193224759 0.0 25 0.0 0.0 0.0 0.14096042913838377 0.0 26 0.0 0.0 0.0 0.15783349796292845 0.0 27 0.0 0.0 0.0 0.25274451010099236 0.0 28 0.0 0.0 0.0 0.32797027527708744 0.0 29 0.0 0.0 0.0 0.41655388660594705 0.0 30 0.0 0.0 0.0 0.5012707529958486 0.0 31 0.0 0.0 0.0 0.5764965181719436 0.0 32 0.0 0.0 0.0 0.6513707610808607 0.0 33 0.0 0.0 0.0 0.780379433135192 0.0 34 0.0 0.0 0.0 0.9487585991134608 0.0 35 0.0 0.0 0.0 1.2141579108328617 0.0 36 0.0 0.0 0.0 1.6159478622173322 0.0 37 0.0 0.0 0.0 2.195959603061056 0.0 38 0.0 0.0 0.0 2.882482590859718 0.0 39 0.0 0.0 0.0 3.596424315498265 0.0 40 0.0 0.0 0.0 4.348330444992038 0.0 41 0.0 0.0 0.0 5.1954991088910525 0.0 42 0.0 0.0 0.0 6.102075035943152 0.0 43 0.0 0.0 0.0 7.033960566232068 0.0 44 0.0 0.0 0.0 7.83543133539794 0.0 45 0.0 0.0 0.0 8.640065804968415 0.0 46 0.0 0.0 0.0 9.421148282637963 0.0 47 0.0 0.0 0.0 10.178678768406584 3.515222671780144E-4 48 0.0 0.0 0.0 10.889105270373351 3.515222671780144E-4 49 0.0 0.0 0.0 11.611483529424172 3.515222671780144E-4 50 0.0 0.0 0.0 12.347922679162112 3.515222671780144E-4 51 0.0 0.0 0.0 13.080495083961093 3.515222671780144E-4 52 0.0 0.0 0.0 13.842946881470207 3.515222671780144E-4 53 0.0 0.0 0.0 14.606453245780854 3.515222671780144E-4 54 0.0 0.0 0.0 15.250793561518154 3.515222671780144E-4 55 0.0 0.0 0.0 15.879666897499622 3.515222671780144E-4 56 0.0 0.0 0.0 16.491315642389367 3.515222671780144E-4 57 0.0 0.0 0.0 17.063242371087995 3.515222671780144E-4 58 0.0 0.0 0.0 17.63059931031331 3.515222671780144E-4 59 0.0 0.0 0.0 18.207095828485254 3.515222671780144E-4 60 0.0 0.0 0.0 18.790974314267938 3.515222671780144E-4 61 0.0 0.0 0.0 19.335482306126682 3.515222671780144E-4 62 0.0 0.0 0.0 19.883505520657206 3.515222671780144E-4 63 0.0 0.0 0.0 20.418873933569323 3.515222671780144E-4 64 0.0 0.0 0.0 20.95529691328297 3.515222671780144E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGAG 20 0.006946687 52.5 22 TCTAACG 20 0.006946687 52.5 11 CCATAGG 20 0.006946687 52.5 4 GTACGAT 20 0.006946687 52.5 20 CGTCAGA 50 1.7072553E-4 35.000004 2 GCAACGC 40 0.0024163842 35.0 17 CAACGCG 45 0.004303065 31.111113 18 TCAGGTC 45 0.004303065 31.111113 3 CGACGAA 70 3.4817123E-5 30.0 22 CCGGTCG 60 4.97498E-4 29.166666 9 CTTCCGA 65 7.944542E-4 26.923077 18 TACGCAT 65 7.944542E-4 26.923077 28 GTCGTGT 65 7.944542E-4 26.923077 12 ACGCATC 70 0.0012242864 25.0 29 GTAGTAC 70 0.0012242864 25.0 3 TAGTACG 75 0.0018296576 23.333334 4 CCAAGAC 100 3.923601E-4 21.000002 3 CCTTTAC 120 5.6983386E-5 20.416666 35 GATATAG 105 5.4526847E-4 20.0 2 ACAGACG 90 0.0052673006 19.444445 45 >>END_MODULE