FastQCFastQC Report
Wed 25 May 2016
SRR1780310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284477
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT97783.4371847284666246No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC28170.9902382266404666No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG21100.7417119837456103TruSeq Adapter, Index 12 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG21020.7388998056081862No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC5950.20915574897091854No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT3020.10615972468776035TruSeq Adapter, Index 12 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACAC200.00694668752.53
CTTTGCG200.00694668752.518
AACCGTA305.871904E-446.66666410
TATCGGT350.001254176740.024
GCGTAGT350.001254176740.022
CGGCATT459.184446E-538.8888942
TATGCCG21150.035.0827443
CAACCGT400.002416384235.09
AGCGGAT400.002416384235.069
CGTATGC21250.034.9176541
CTCGTAT21250.034.9176539
GTATGCC21500.034.67441642
TCGTATG21400.034.67289740
CGTCTTC21100.034.66824748
CCGTCTT21300.034.50704647
ATGCCGT21450.034.42890544
GCCGTCT21350.034.42622846
TCTCGTA20700.034.32367338
CTGCTTG21550.034.2691454
TGCCGTC21650.034.11085545
Produced by FastQC (version 0.11.5)