##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780310_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 284477 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.771978050949635 34.0 34.0 34.0 34.0 34.0 2 33.76978806722512 34.0 34.0 34.0 34.0 34.0 3 33.771341795646045 34.0 34.0 34.0 34.0 34.0 4 33.792629984146345 34.0 34.0 34.0 34.0 34.0 5 33.77934947289236 34.0 34.0 34.0 34.0 34.0 6 37.54473648133241 38.0 38.0 38.0 37.0 38.0 7 37.52691781760915 38.0 38.0 38.0 37.0 38.0 8 37.45748865461883 38.0 38.0 38.0 37.0 38.0 9 37.47470621526521 38.0 38.0 38.0 37.0 38.0 10-11 37.42860758514748 38.0 38.0 38.0 37.0 38.0 12-13 37.409806416687466 38.0 38.0 38.0 37.0 38.0 14-15 37.331664774305125 38.0 38.0 38.0 37.0 38.0 16-17 37.37308288543538 38.0 38.0 38.0 37.0 38.0 18-19 37.35623793839221 38.0 38.0 38.0 36.5 38.0 20-21 37.364015368553524 38.0 38.0 38.0 37.0 38.0 22-23 37.34631270717844 38.0 38.0 38.0 37.0 38.0 24-25 37.370592350172416 38.0 38.0 38.0 37.0 38.0 26-27 37.29423995613002 38.0 38.0 38.0 36.5 38.0 28-29 37.25967301398707 38.0 38.0 38.0 36.0 38.0 30-31 37.24516744763197 38.0 38.0 38.0 36.0 38.0 32-33 37.243476625526846 38.0 38.0 38.0 36.0 38.0 34-35 37.21476252913241 38.0 38.0 38.0 36.0 38.0 36-37 37.22282820755281 38.0 38.0 38.0 36.0 38.0 38-39 36.96353659522563 38.0 38.0 38.0 36.0 38.0 40-41 37.0773120498318 38.0 38.0 38.0 36.0 38.0 42-43 37.15405463359077 38.0 38.0 38.0 36.0 38.0 44-45 37.20825409435561 38.0 38.0 38.0 36.0 38.0 46-47 37.15283660893499 38.0 38.0 38.0 36.0 38.0 48-49 37.16698889541158 38.0 38.0 38.0 36.0 38.0 50-51 37.06935007048021 38.0 38.0 38.0 36.0 38.0 52-53 37.08791220379855 38.0 38.0 38.0 36.0 38.0 54-55 37.05430843266767 38.0 38.0 38.0 36.0 38.0 56-57 37.09255757055931 38.0 38.0 38.0 36.0 38.0 58-59 37.01505042586923 38.0 38.0 38.0 36.0 38.0 60-61 37.03781149266901 38.0 38.0 38.0 36.0 38.0 62-63 37.00472797449355 38.0 38.0 38.0 36.0 38.0 64-65 36.96971987190528 38.0 38.0 38.0 36.0 38.0 66-67 36.781332058479244 38.0 38.0 38.0 35.5 38.0 68-69 36.75297124196332 38.0 38.0 38.0 35.0 38.0 70-71 36.37500571223684 38.0 37.5 38.0 34.5 38.0 72-73 35.81993623386073 38.0 37.0 38.0 31.5 38.0 74-75 35.64744074213381 38.0 37.0 38.0 31.0 38.0 76 35.01437726072758 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 9.968251248153592E-4 1101 2 -0.04845323666415169 1101 3 -0.06030373015008195 1101 4 0.02990989627846119 1101 5 -0.0356223962514548 1101 6 -0.05030261335890174 1101 7 0.06445147295098508 1101 8 -0.04742889115075144 1101 9 0.11392781188797585 1101 10-11 0.13494284912849253 1101 12-13 0.06315757670166278 1101 14-15 0.1721752146401201 1101 16-17 0.08735460958141772 1101 18-19 0.11367334135513119 1101 20-21 0.19002505737131514 1101 22-23 0.11626752850484223 1101 24-25 -0.06792189328702847 1101 26-27 0.013348973517217644 1101 28-29 -0.25395052392170214 1101 30-31 -0.11058835006453194 1101 32-33 -0.06542599885722922 1101 34-35 0.20753502166301274 1101 36-37 0.0729548996863798 1101 38-39 0.3182319029227969 1101 40-41 0.2424680427316801 1101 42-43 0.18673556761655874 1101 44-45 0.1336397134625571 1101 46-47 0.11911028283665104 1101 48-49 0.10460865064799663 1101 50-51 0.11364917842065125 1101 52-53 0.09255857771369591 1101 54-55 -0.011376813179346357 1101 56-57 -0.1375000463635203 1101 58-59 -0.001743065649179698 1101 60-61 0.09511466247032985 1101 62-63 0.05650521476950843 1101 64-65 -0.03730232157055724 1101 66-67 -0.04661140044219536 1101 68-69 -0.38512948557909965 1101 70-71 -0.4977057398532878 1101 72-73 -0.18543038454982508 1101 74-75 -0.32727854458095607 1101 76 -1.1418977749905252 1102 1 0.012204884709539954 1102 2 0.05462658483712346 1102 3 -0.07605807886123728 1102 4 -0.023220616945287986 1102 5 -0.007893624907048036 1102 6 0.022936946940298242 1102 7 -0.0702687646215594 1102 8 -0.06911856201379152 1102 9 -0.10311258981398908 1102 10-11 -0.07677492171298894 1102 12-13 0.07634939896930604 1102 14-15 0.049263753521074705 1102 16-17 -0.10502047723314689 1102 18-19 0.032877920374353664 1102 20-21 -0.08043923090892235 1102 22-23 -0.07940850748229167 1102 24-25 -0.06153952602350188 1102 26-27 0.008868543715273347 1102 28-29 0.016204425107176235 1102 30-31 0.09930332132537956 1102 32-33 0.10048960541204366 1102 34-35 0.09062873349454037 1102 36-37 0.11767737248447929 1102 38-39 -0.00537088152962184 1102 40-41 0.08900583800097905 1102 42-43 0.00650454108922105 1102 44-45 0.06125233290742216 1102 46-47 0.02239686270205965 1102 48-49 0.004622221660312675 1102 50-51 0.02167363817461876 1102 52-53 -0.03633809898487783 1102 54-55 -0.034856660226054714 1102 56-57 0.009192618105181793 1102 58-59 0.14437881800733265 1102 60-61 0.12672713822212955 1102 62-63 0.04575817045616759 1102 64-65 0.18981256104019195 1102 66-67 0.24197418075636534 1102 68-69 0.11394050092972208 1102 70-71 0.21012034363238996 1102 72-73 -0.11653354819228667 1102 74-75 -0.14711337737810481 1102 76 0.30795334302140276 1103 1 -0.017033027233708253 1103 2 -0.06712542783035502 1103 3 -0.0838195031363611 1103 4 -0.04802528344137613 1103 5 0.05713287073387363 1103 6 0.006284927968756904 1103 7 0.0675493592126557 1103 8 -0.023427023931638757 1103 9 -0.02478264955433218 1103 10-11 0.022536451202498142 1103 12-13 0.11401807923051166 1103 14-15 0.08387774040119211 1103 16-17 0.03266322155091217 1103 18-19 0.020313349996804675 1103 20-21 0.011497305634293298 1103 22-23 0.08804802223769315 1103 24-25 0.05003697388193018 1103 26-27 0.07019788694228879 1103 28-29 -0.10173455148247257 1103 30-31 -0.0582975345450123 1103 32-33 0.09145995031891374 1103 34-35 -0.050140251957891735 1103 36-37 0.04880353920120939 1103 38-39 -0.017132609883475425 1103 40-41 0.10042918605617501 1103 42-43 -0.05177659824187941 1103 44-45 -0.11466185014055696 1103 46-47 0.016278205246749167 1103 48-49 0.02361416357386048 1103 50-51 -0.10486946869422553 1103 52-53 0.012124449269087734 1103 54-55 0.047861099084506975 1103 56-57 -0.2162511699741927 1103 58-59 0.0497964256493475 1103 60-61 0.02228443666798796 1103 62-63 -0.09580624046544983 1103 64-65 0.19528412411612805 1103 66-67 -0.15908434698340557 1103 68-69 -0.07482100863578722 1103 70-71 -0.21762480130562523 1103 72-73 -0.0201785993108885 1103 74-75 0.0932942364567424 1103 76 0.2840442254801374 1104 1 0.0699274271966317 1104 2 0.011271040927560705 1104 3 -0.006888355120871381 1104 4 -0.0287083761726592 1104 5 0.03401698376256235 1104 6 0.07780705250292641 1104 7 0.08692596473383674 1104 8 -0.1408824311460819 1104 9 -0.1258572180987798 1104 10-11 0.012811063163852054 1104 12-13 -0.019461786177068063 1104 14-15 -0.024308156338918252 1104 16-17 0.05086033432633741 1104 18-19 0.07325936830427082 1104 20-21 -0.0436457892678348 1104 22-23 0.059327735686082406 1104 24-25 0.039123047907764885 1104 26-27 0.11152445447367398 1104 28-29 0.03516077758153813 1104 30-31 -0.06710754937912355 1104 32-33 -0.05715432660861808 1104 34-35 -0.0024404437159049053 1104 36-37 0.0391866934397882 1104 38-39 0.03818867525708214 1104 40-41 0.04817331572801464 1104 42-43 -0.047233199421370387 1104 44-45 0.06534194883914068 1104 46-47 -0.10772591935977971 1104 48-49 -0.10749314855755188 1104 50-51 -0.19429925993124897 1104 52-53 -0.07814202202148124 1104 54-55 -0.21527703905363182 1104 56-57 -0.3634191210578095 1104 58-59 0.025283961377851938 1104 60-61 -0.05807483826959725 1104 62-63 -0.057294535989896644 1104 64-65 -0.25453497208851417 1104 66-67 -0.40165462810789876 1104 68-69 -0.24733448596854402 1104 70-71 -0.1682018844128308 1104 72-73 0.09633610816766947 1104 74-75 0.16261747535828874 1104 76 0.39760296494249303 1105 1 -0.05859783719819944 1105 2 -0.030606162362680323 1105 3 -0.02302378402804095 1105 4 -0.05710144315268906 1105 5 0.028956760495816525 1105 6 0.0201028809400583 1105 7 0.10262807989247591 1105 8 -0.03722496933644237 1105 9 0.0010816773183748296 1105 10-11 -0.08522066421569718 1105 12-13 -0.07639847830397883 1105 14-15 -0.11364423154456205 1105 16-17 -0.04864025321772658 1105 18-19 -0.09420905120219203 1105 20-21 0.04578208969809339 1105 22-23 0.0032686875074645627 1105 24-25 -0.08502501319681954 1105 26-27 -0.00920850674841489 1105 28-29 0.12319323939117055 1105 30-31 0.09296148704861196 1105 32-33 0.11383568514226994 1105 34-35 -0.05008659530228243 1105 36-37 -0.049756461671847774 1105 38-39 -0.22213741169943546 1105 40-41 -0.09917539167503975 1105 42-43 -0.21569677168929502 1105 44-45 -0.1661504130879976 1105 46-47 -0.19575470901899905 1105 48-49 -0.1050181191803432 1105 50-51 -0.254706134196816 1105 52-53 -0.2750978093305321 1105 54-55 -0.2086304468092166 1105 56-57 0.024345273771807285 1105 58-59 -0.09141697869265641 1105 60-61 0.047202041871408085 1105 62-63 -0.09347990614265456 1105 64-65 -0.17050148207675875 1105 66-67 -0.16510571506442062 1105 68-69 -0.05090014401789489 1105 70-71 -0.285986108854658 1105 72-73 -0.053480284311767434 1105 74-75 -0.11550018034088794 1105 76 -0.16677717606808784 1106 1 0.006534191028521263 1106 2 0.06484398559998539 1106 3 -0.043205038296193266 1106 4 0.04640128846580183 1106 5 -0.04537313934483933 1106 6 0.012128546772068205 1106 7 -0.13472395743964682 1106 8 -0.0489339381504621 1106 9 0.07235984713398835 1106 10-11 0.051957801525254865 1106 12-13 0.023643491875851907 1106 14-15 -0.16893105148537302 1106 16-17 0.03501556285329599 1106 18-19 -0.020928405857326027 1106 20-21 0.030420292520759062 1106 22-23 -0.09897493871606144 1106 24-25 0.010638542459908251 1106 26-27 -0.1003122513420891 1106 28-29 0.12187366848290537 1106 30-31 -0.04926051386193109 1106 32-33 -0.07952591285454247 1106 34-35 -0.05426582931092838 1106 36-37 -0.009539781726481067 1106 38-39 -0.12529300013557787 1106 40-41 -0.0510519566647929 1106 42-43 0.10058765808534531 1106 44-45 -0.05734094347726426 1106 46-47 -0.04262757494205971 1106 48-49 -0.06355300422472965 1106 50-51 -0.09906790291978496 1106 52-53 -0.10071171213044039 1106 54-55 -0.09662697537739717 1106 56-57 -0.05892640844991348 1106 58-59 0.03556059927228716 1106 60-61 0.10762843044414439 1106 62-63 0.17934760353182355 1106 64-65 0.10202014323623843 1106 66-67 -0.09353944486182542 1106 68-69 0.005997904013064215 1106 70-71 0.010639915502267172 1106 72-73 0.033625622813211464 1106 74-75 0.058124762750395575 1106 76 0.39799917266854123 1107 1 -0.09978587924058502 1107 2 0.01588384395515874 1107 3 0.0693621357927583 1107 4 0.11107169451889831 1107 5 0.07639264872739915 1107 6 -0.0645329096520939 1107 7 0.03162052719590491 1107 8 0.21778264079508602 1107 9 0.14618391153811672 1107 10-11 0.040741371279104044 1107 12-13 0.07990805922977273 1107 14-15 0.19787578453722432 1107 16-17 0.11359195630498675 1107 18-19 -0.07457257856994914 1107 20-21 0.044722235295701296 1107 22-23 0.03568533502915017 1107 24-25 0.15350235455920114 1107 26-27 0.1234500855208367 1107 28-29 0.1910517412003685 1107 30-31 0.062422244615660816 1107 32-33 0.11773767239170496 1107 34-35 -0.010317376130494438 1107 36-37 0.1185647504928724 1107 38-39 0.11074481792551438 1107 40-41 0.12235661395359188 1107 42-43 0.1810746659076301 1107 44-45 0.12773707183531968 1107 46-47 0.15132831320832452 1107 48-49 0.13046340112290977 1107 50-51 0.1907109153695501 1107 52-53 0.20762773055804473 1107 54-55 0.255111943059255 1107 56-57 -0.10159881329958864 1107 58-59 0.05109709287129505 1107 60-61 -0.19338670231329758 1107 62-63 -0.0644770487536519 1107 64-65 0.2037123769778617 1107 66-67 0.189098344151013 1107 68-69 0.058713570013466665 1107 70-71 -0.02348054484340878 1107 72-73 -0.1029694721815062 1107 74-75 -0.15250534230745671 1107 76 -0.28650392927008284 1108 1 -0.0240115252328863 1108 2 0.06168992342191615 1108 3 0.02200360962014969 1108 4 -0.06853499524456197 1108 5 -0.07061622087064023 1108 6 -0.1112291535144081 1108 7 0.07711682945300424 1108 8 0.05366481151437341 1108 9 0.009299771775189924 1108 10-11 -0.23034840358052122 1108 12-13 -0.2435821329389185 1108 14-15 -0.00426007591348565 1108 16-17 -0.037931827107328786 1108 18-19 0.1637288809436015 1108 20-21 0.12258135431780204 1108 22-23 0.004246508906746271 1108 24-25 0.025897288455588807 1108 26-27 -0.06478318740000333 1108 28-29 0.05248430056543896 1108 30-31 0.02601185034404807 1108 32-33 -0.15341352381120288 1108 34-35 -0.019241274195984204 1108 36-37 0.05129057629838485 1108 38-39 0.13461724668565722 1108 40-41 0.034324696219023565 1108 42-43 0.043392938846274376 1108 44-45 0.05447832240948003 1108 46-47 0.07946356122592846 1108 48-49 0.11196667514755632 1108 50-51 -0.05517563495855882 1108 52-53 -0.05121516254745728 1108 54-55 0.13014621438848195 1108 56-57 0.23301309282723537 1108 58-59 -0.033414224678836035 1108 60-61 -0.005503263999912633 1108 62-63 -0.04035893462032902 1108 64-65 0.09597132936118413 1108 66-67 -0.0846734960324298 1108 68-69 0.0571967298834366 1108 70-71 0.3689875961728717 1108 72-73 0.27643372600113736 1108 74-75 0.3901744798020843 1108 76 0.5353278866296733 1109 1 0.07072661347968534 1109 2 0.06748289794587237 1109 3 0.029234864873316724 1109 4 -0.10680340668704957 1109 5 -0.01474064483377191 1109 6 -0.19709849820907266 1109 7 0.021661506727440383 1109 8 0.007918789429332662 1109 9 0.005289250950909263 1109 10-11 -0.09249626028862679 1109 12-13 0.020061608185038438 1109 14-15 0.050626455692466266 1109 16-17 0.0726781198538049 1109 18-19 0.11392705211711984 1109 20-21 0.0641244810024375 1109 22-23 0.021370019905781135 1109 24-25 0.03962435566713651 1109 26-27 -0.020384874209845805 1109 28-29 -0.08015090506886224 1109 30-31 -0.053412402619457566 1109 32-33 -0.08383261780303286 1109 34-35 -0.08299478838315366 1109 36-37 -0.12685878752505175 1109 38-39 -0.20764465807624077 1109 40-41 -0.09705699522810107 1109 42-43 -0.049263859113324315 1109 44-45 0.013431167089656526 1109 46-47 0.21927567901278167 1109 48-49 -0.04945769374789677 1109 50-51 -0.014918266034122496 1109 52-53 -0.022416942102857718 1109 54-55 -0.16943280772553493 1109 56-57 0.04599674642257412 1109 58-59 0.0755418805873802 1109 60-61 0.21009211901026248 1109 62-63 0.036297443085956616 1109 64-65 0.12335662919019086 1109 66-67 0.1224045116770398 1109 68-69 0.1430207886609267 1109 70-71 0.5182083161102753 1109 72-73 0.20018673298603318 1109 74-75 0.3665080594908119 1109 76 0.40349514744757187 1110 1 -0.10413308750133865 1110 2 -0.07416020705348814 1110 3 -0.06685389835335087 1110 4 0.01766110087410766 1110 5 -0.009337526125811735 1110 6 -0.22394808909285047 1110 7 -0.15120080745668218 1110 8 0.04344871274681594 1110 9 -0.029758729171611265 1110 10-11 -0.058912851986349324 1110 12-13 0.022128608998990273 1110 14-15 -0.30592582978926686 1110 16-17 -0.06965041546727946 1110 18-19 0.03319888949877736 1110 20-21 0.02635887675276649 1110 22-23 -0.2310530833799973 1110 24-25 0.012180368176238687 1110 26-27 -0.08214321718204332 1110 28-29 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42-43 0.06765169895409429 2115 44-45 0.18048910408063534 2115 46-47 0.15628296823400945 2115 48-49 0.12679147299122917 2115 50-51 0.10385401463712896 2115 52-53 0.21360963529621557 2115 54-55 0.14227559444239546 2115 56-57 0.19056026263855586 2115 58-59 0.19300086966079277 2115 60-61 0.24314902710521125 2115 62-63 0.10451977157913461 2115 64-65 0.11686081723181019 2115 66-67 0.004275291029564698 2115 68-69 0.032264115328182186 2115 70-71 0.05834069590333968 2115 72-73 0.17939868556987193 2115 74-75 0.15365156875087393 2115 76 0.22131171412293327 2116 1 -0.04782812362979172 2116 2 -0.02873037781401422 2116 3 0.00993082626524 2116 4 0.005327234240901646 2116 5 -1.2503226921722899E-4 2116 6 -0.02312931693737852 2116 7 0.019915253234934482 2116 8 0.09782438590719522 2116 9 0.008199673438426203 2116 10-11 0.1070793471959206 2116 12-13 0.002493239714972617 2116 14-15 0.07822784422857865 2116 16-17 -0.24995544207999387 2116 18-19 -0.06727585195535113 2116 20-21 -0.08334927227840438 2116 22-23 -0.0017916514175553289 2116 24-25 -0.13010090045003864 2116 26-27 0.029473308839847334 2116 28-29 0.00680722718074378 2116 30-31 -0.03818295549389461 2116 32-33 0.06532918052056402 2116 34-35 0.15683989961954836 2116 36-37 -0.03326489200392757 2116 38-39 0.012007572894880525 2116 40-41 0.0441937456067123 2116 42-43 2.6094826962719253E-4 2116 44-45 -0.09432854842977889 2116 46-47 0.05085746717635686 2116 48-49 -0.02188805527528359 2116 50-51 0.009804450077979254 2116 52-53 -0.12108555610674898 2116 54-55 0.00302295385265694 2116 56-57 0.02644929066099877 2116 58-59 0.03959551702799047 2116 60-61 -0.017275377391399616 2116 62-63 0.19694218104209682 2116 64-65 -0.031657764171932 2116 66-67 0.10648185483172767 2116 68-69 0.2088122119104483 2116 70-71 0.14353110842252192 2116 72-73 -0.0270802215478696 2116 74-75 0.09873331560798704 2116 76 0.21139667991730704 2117 1 -0.05391069664149484 2117 2 -0.02141879176227235 2117 3 0.009649611417096082 2117 4 0.006462508998225758 2117 5 -0.028288178542595688 2117 6 0.05780848250435611 2117 7 0.0985304263383 2117 8 -0.39374958401322857 2117 9 -0.035524027765589494 2117 10-11 0.08967006502285813 2117 12-13 0.02765676094147551 2117 14-15 0.020011162980395625 2117 16-17 0.00777796624397098 2117 18-19 0.12157554162045159 2117 20-21 0.01749749687544977 2117 22-23 0.10038307674142288 2117 24-25 -0.1487183649336501 2117 26-27 -0.05919269124347437 2117 28-29 -0.4372830948623374 2117 30-31 0.005322023050453595 2117 32-33 -0.012041356495650746 2117 34-35 0.05479536351989367 2117 36-37 0.005256334509425642 2117 38-39 0.11411460118301164 2117 40-41 -0.10619891364421363 2117 42-43 -0.06713166931984205 2117 44-45 -0.06757668407949069 2117 46-47 -0.021539733173597142 2117 48-49 -0.1654117814761804 2117 50-51 0.14477195413997634 2117 52-53 -0.0882771571566181 2117 54-55 -0.03877911255570865 2117 56-57 -0.1955333781720654 2117 58-59 -0.04473248363859739 2117 60-61 -0.03947051966527937 2117 62-63 0.07766542397006049 2117 64-65 -0.13667067922421694 2117 66-67 0.016467065013792137 2117 68-69 -0.16572430435835628 2117 70-71 0.010047793836847063 2117 72-73 -0.06562227879071258 2117 74-75 -0.03741112467031371 2117 76 -0.3675622002226788 2118 1 0.046252316054157916 2118 2 -0.1253601071918382 2118 3 -0.09214698189386183 2118 4 -0.1646973677752328 2118 5 0.03885785596636282 2118 6 0.10203258351697286 2118 7 0.04825896875613722 2118 8 0.006118486220373143 2118 9 -0.0035017033150666066 2118 10-11 0.014095376197516885 2118 12-13 0.06296006125783293 2118 14-15 -0.012710527378374081 2118 16-17 0.013015439823476527 2118 18-19 -0.1634652806266459 2118 20-21 -0.10379327356675105 2118 22-23 0.08111166060316322 2118 24-25 0.021400233709755412 2118 26-27 -0.030986659773731162 2118 28-29 0.037905820274360735 2118 30-31 0.12379288107744202 2118 32-33 0.06031797598118516 2118 34-35 0.1319449207608372 2118 36-37 0.1152363723615224 2118 38-39 0.004441938964653502 2118 40-41 -0.006766349786062165 2118 42-43 0.17101710048689256 2118 44-45 0.12044568181493531 2118 46-47 0.13069409334221405 2118 48-49 0.13259752958796156 2118 50-51 0.1359703046725258 2118 52-53 0.08013015354834607 2118 54-55 0.01899541878279365 2118 56-57 0.01671813107683562 2118 58-59 0.12880523722288473 2118 60-61 0.008642401163044156 2118 62-63 0.15531748856730587 2118 64-65 0.005234980631833253 2118 66-67 0.17680490191821008 2118 68-69 0.1417100415877357 2118 70-71 -0.057588660222577914 2118 72-73 -0.09332234924535499 2118 74-75 -0.05146233552308388 2118 76 -0.39940148075647386 2119 1 0.0529958933159449 2119 2 -0.01670034636585882 2119 3 -0.039599162407952804 2119 4 0.037858451411111105 2119 5 -0.13822657661658866 2119 6 -0.04354324172872026 2119 7 -0.12071172510724182 2119 8 2.9061934900909137E-4 2119 9 0.007607667908473559 2119 10-11 -0.04974223328774485 2119 12-13 -0.22319518058842647 2119 14-15 -0.5418158204567547 2119 16-17 -0.05465606864769512 2119 18-19 -7.522247751197142E-4 2119 20-21 -0.016139534642931608 2119 22-23 0.06943380002943655 2119 24-25 -0.17923735943569596 2119 26-27 0.04363182259798748 2119 28-29 0.05703786979761105 2119 30-31 -0.08206579315092455 2119 32-33 -0.25627867912279356 2119 34-35 -0.028247378421148994 2119 36-37 -0.011015921169608589 2119 38-39 0.08078971828185644 2119 40-41 0.0915610319970881 2119 42-43 -0.13973381607453206 2119 44-45 -0.15443764170316143 2119 46-47 -0.1865042413584419 2119 48-49 -0.13639920585343646 2119 50-51 -0.07943439506637873 2119 52-53 -0.024391889881520967 2119 54-55 0.07364698927717228 2119 56-57 -0.14313035119277373 2119 58-59 -0.19991777229846264 2119 60-61 0.010516373541953783 2119 62-63 0.023401577333331147 2119 64-65 -0.3989005240811778 2119 66-67 -0.20778239864245762 2119 68-69 -0.2206210711195311 2119 70-71 -0.06581984135281971 2119 72-73 -0.20511390768912463 2119 74-75 -0.2517390911169173 2119 76 -1.7131003720320308 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 2.0 20 7.0 21 3.0 22 16.0 23 19.0 24 62.0 25 108.0 26 211.0 27 388.0 28 570.0 29 1019.0 30 1623.0 31 2752.0 32 4334.0 33 7175.0 34 11601.0 35 19988.0 36 36062.0 37 198536.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.07134847456912 18.763204055160873 12.545126671048978 29.620320799221027 2 19.56783852473135 21.015055698703232 35.1416810497861 24.275424726779317 3 19.301384646210415 21.519841674370863 31.41519349543197 27.763580183986754 4 14.814905950217415 20.928932743244623 34.77820702552403 29.47795428101393 5 14.836348808515275 32.24056777876594 37.08665375408205 15.836429658636728 6 28.884334706618485 37.05549853063176 18.054950153967294 16.00521660878246 7 26.496693933077193 27.942505017980363 22.99693824105288 22.563862807889564 8 23.900701989967555 35.917842215715154 19.35903429802761 20.822421496289685 9 23.066539649954127 17.498778460121557 20.72329221694548 38.71138967297883 10-11 25.373756050577022 26.190342277231547 25.794000920988342 22.641900751203085 12-13 24.909395135634867 22.0052939253437 26.178214759013912 26.90709618000752 14-15 22.595851334202766 21.94658970672497 22.81643155685697 32.64112740221529 16-17 25.222601475690475 26.51831255250864 22.719411411115836 25.539674560685043 18-19 25.670968127476034 25.425957107252962 24.04517764177772 24.857897123493288 20-21 23.31910839892153 24.819581196370883 23.897011006162185 27.9642993985454 22-23 22.65156761355048 26.718680244800108 24.258727419088363 26.37102472256105 24-25 25.170400419014545 26.872471236690487 23.641805840190948 24.315322504104024 26-27 25.300815180137587 27.045068669874894 24.694087746988334 22.96002840299919 28-29 23.34969083616602 25.486243176073987 26.322690410824073 24.84137557693592 30-31 22.38071970668982 27.02608646744728 25.383598674058007 25.20959515180489 32-33 21.641819901081636 25.37182267810755 29.06526713934694 23.92109028146388 34-35 24.592146289506708 25.73723007483909 27.08004513545911 22.590578500195093 36-37 23.438450208628467 29.28215637819578 24.760349694351387 22.51904371882437 38-39 21.69402095775757 26.1959666335064 27.611898325699443 24.49811408303659 40-41 21.908625301869748 23.29959891309315 28.6678712163022 26.123904568734908 42-43 23.885235010211723 23.05529093740443 28.49914052805675 24.5603335243271 44-45 21.443034058992467 25.485891653806807 29.85830137410054 23.21277291310018 46-47 22.790946192486565 23.97733384421236 28.7046052932223 24.527114670078774 48-49 22.468424512350733 23.505942483926646 27.29324339050257 26.732389613220047 50-51 21.321934637949642 24.000007030445346 28.804964900501624 25.873093431103396 52-53 19.03897327376203 24.217775074962123 32.42582001356876 24.31743163770709 54-55 19.221413330427417 24.47842883607462 30.17361684775922 26.126540985738743 56-57 21.150040249299593 24.70498493727085 27.555830524084545 26.589144289345008 58-59 19.873311374909044 24.688463390713487 30.23776966151921 25.20045557285826 60-61 21.556224229023787 26.498978827813847 27.426821852030216 24.517975091132147 62-63 19.447969431623648 30.42390070198997 25.123647957479868 25.004481908906516 64-65 19.23248628184352 31.206037746461053 24.274721682244962 25.286754289450464 66-67 19.3639556097681 30.848188078473832 24.093863475781873 25.693992835976193 68-69 19.37819226158881 31.36334396102321 24.109681977804883 25.14878179958309 70-71 19.542177399227352 30.726561374030236 24.16012542314493 25.57113580359748 72-73 19.328276099649532 29.568998548213038 24.82520555264573 26.2775197994917 74-75 19.201552322331857 29.13416550371383 25.46638216797843 26.197900005975878 76 19.265177852691078 29.187245366057713 25.572893414933368 25.97468336631784 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 6.0 12 11.0 13 11.0 14 15.0 15 40.0 16 80.0 17 99.0 18 154.5 19 260.5 20 407.5 21 504.0 22 604.5 23 821.0 24 1128.5 25 1320.0 26 1533.0 27 1979.5 28 2576.5 29 2940.0 30 3289.0 31 3947.0 32 4769.0 33 5282.0 34 5767.5 35 6768.0 36 7725.5 37 8168.0 38 11015.0 39 15527.0 40 17040.0 41 16191.0 42 15494.0 43 14785.0 44 14291.0 45 13994.0 46 13482.0 47 13565.5 48 13166.0 49 12172.0 50 11661.0 51 11010.0 52 9543.0 53 8253.0 54 7779.0 55 7544.0 56 6944.0 57 6136.0 58 5693.0 59 5359.5 60 4865.0 61 4533.0 62 4362.0 63 4216.0 64 4158.0 65 4050.0 66 3859.0 67 3864.0 68 3789.5 69 3529.0 70 3165.5 71 2988.0 72 2791.0 73 2350.0 74 1817.5 75 1529.0 76 1300.5 77 860.5 78 479.5 79 310.0 80 206.0 81 62.0 82 15.5 83 9.0 84 5.0 85 0.5 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 3.515222671780144E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 284477.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.54683735322545 #Duplication Level Percentage of deduplicated Percentage of total 1 66.77795790413967 35.75748450673502 2 20.491741817681923 21.945359323914047 3 6.400317104479697 10.281502170079234 4 2.1987896000260814 4.709529163462408 5 1.0384701066712496 2.7803394949056046 6 0.6706215932451052 2.1545799227433937 7 0.45175277775584427 1.6932952760051998 8 0.35624701617833515 1.526072082629855 9 0.27845307523948404 1.341925337731869 >10 1.3185855288178718 10.991286868805021 >50 0.009844312941226011 0.3514212159453878 >100 0.003937725176490405 0.35177263716133317 >500 6.562875294150674E-4 0.20909562348750574 >1k 0.0019688625882452026 2.470139726880131 >5k 6.562875294150674E-4 3.436196649514002 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 9778 3.4371847284666246 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 2817 0.9902382266404666 No Hit CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG 2110 0.7417119837456103 TruSeq Adapter, Index 12 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 2102 0.7388998056081862 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 595 0.20915574897091854 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 302 0.10615972468776035 TruSeq Adapter, Index 12 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006327400809204259 0.0 2 0.0 0.0 0.0 0.013357846152764547 0.0 3 0.0 0.0 0.0 0.014763935221476605 0.0 4 0.0 0.0 0.0 0.08436534412272345 0.0 5 0.0 0.0 0.0 0.09350492306935183 0.0 6 0.0 0.0 0.0 0.09561405667241991 0.0 7 0.0 0.0 0.0 0.09666862347395395 0.0 8 0.0 0.0 0.0 0.09702014574113198 0.0 9 0.0 0.0 0.0 0.10440211335187027 0.0 10 0.0 0.0 0.0 0.10862038055800645 0.0 11 0.0 0.0 0.0 0.1100264696267185 0.0 12 0.0 0.0 0.0 0.1149477813672107 0.0 13 0.0 0.0 0.0 0.11670539270310078 0.0 14 0.0 0.0 0.0 0.11670539270310078 0.0 15 0.0 0.0 0.0 0.11740843723745681 0.0 16 0.0 0.0 0.0 0.11775995950463482 0.0 17 0.0 0.0 0.0 0.11951757084052489 0.0 18 0.0 0.0 0.0 0.12197822671077099 0.0 19 0.0 0.0 0.0 0.12725106071844122 0.0 20 0.0 0.0 0.0 0.13006323885586532 0.0 21 0.0 0.0 0.0 0.1318208501917554 0.0 22 0.0 0.0 0.0 0.1346330283291795 0.0 23 0.0 0.0 0.0 0.1392028178024937 0.0 24 0.0 0.0 0.0 0.14447565181016392 0.0 25 0.0 0.0 0.0 0.1490454412834781 0.0 26 0.0 0.0 0.0 0.16556698784084478 0.0 27 0.0 0.0 0.0 0.2622356113147987 0.0 28 0.0 0.0 0.0 0.34132812142985197 0.0 29 0.0 0.0 0.0 0.4323723886289577 0.0 30 0.0 0.0 0.0 0.5170892550188592 0.0 31 0.0 0.0 0.0 0.5933695869964883 0.0 32 0.0 0.0 0.0 0.6707044857756514 0.0 33 0.0 0.0 0.0 0.8018222914330508 0.0 34 0.0 0.0 0.0 0.9716075464800318 0.0 35 0.0 0.0 0.0 1.2415766476727468 0.0 36 0.0 0.0 0.0 1.6479363885305314 0.0 37 0.0 0.0 0.0 2.2356816192521713 0.0 38 0.0 0.0 0.0 2.929586574661572 0.0 39 0.0 0.0 0.0 3.6498557001093235 0.0 40 0.0 0.0 0.0 4.404925530007699 0.0 41 0.0 0.0 0.0 5.263342906456409 0.0 42 0.0 0.0 0.0 6.181519068325383 0.0 43 0.0 0.0 0.0 7.125356355698352 0.0 44 0.0 0.0 0.0 7.9352636592764965 0.0 45 0.0 0.0 0.0 8.753959019534092 0.0 46 0.0 0.0 0.0 9.546290209753337 0.0 47 0.0 0.0 0.0 10.310148096331162 0.0 48 0.0 0.0 0.0 11.03463548898505 0.0 49 0.0 0.0 0.0 11.768965505119922 0.0 50 0.0 0.0 0.0 12.510677488865532 0.0 51 0.0 0.0 0.0 13.251334905809609 0.0 52 0.0 0.0 0.0 14.018708015059214 0.0 53 0.0 0.0 0.0 14.789947869247777 0.0 54 0.0 0.0 0.0 15.439561018992748 0.0 55 0.0 0.0 0.0 16.073707188981885 0.0 56 0.0 0.0 0.0 16.696604646421328 0.0 57 0.0 0.0 0.0 17.27169507552456 0.0 58 0.0 0.0 0.0 17.84151267062012 0.0 59 0.0 0.0 0.0 18.425391156402803 0.0 60 0.0 0.0 0.0 19.015948565261866 0.0 61 0.0 0.0 0.0 19.554480678578585 0.0 62 0.0 0.0 0.0 20.110237382987027 0.0 63 0.0 0.0 0.0 20.645254273631963 0.0 64 0.0 0.0 0.0 21.185543998284572 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTACAC 20 0.006946687 52.5 3 CTTTGCG 20 0.006946687 52.5 18 AACCGTA 30 5.871904E-4 46.666664 10 TATCGGT 35 0.0012541767 40.0 24 GCGTAGT 35 0.0012541767 40.0 22 CGGCATT 45 9.184446E-5 38.88889 42 TATGCCG 2115 0.0 35.08274 43 CAACCGT 40 0.0024163842 35.0 9 AGCGGAT 40 0.0024163842 35.0 69 CGTATGC 2125 0.0 34.91765 41 CTCGTAT 2125 0.0 34.91765 39 GTATGCC 2150 0.0 34.674416 42 TCGTATG 2140 0.0 34.672897 40 CGTCTTC 2110 0.0 34.668247 48 CCGTCTT 2130 0.0 34.507046 47 ATGCCGT 2145 0.0 34.428905 44 GCCGTCT 2135 0.0 34.426228 46 TCTCGTA 2070 0.0 34.323673 38 CTGCTTG 2155 0.0 34.26914 54 TGCCGTC 2165 0.0 34.110855 45 >>END_MODULE