FastQCFastQC Report
Wed 25 May 2016
SRR1780309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830989
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA300.00571765429.36092638
CGCAATC454.0420116E-529.36092412
CTCGTAA500.002602788921.9663561
TATACCG500.002603698521.9650366
CAAACGG550.004522322719.9694182
ATACCGC550.00452389819.9682147
ACGAGAT600.00747848718.3041958
AGATCTC2600.016.93899540
GACGAAA1954.9112714E-1116.9257223
AAACGGG1451.2724013E-716.6611253
TAGATCT2007.094059E-1116.5155239
GTAGATC2301.8189894E-1216.27616538
CGACGAA2750.016.00153522
ATTTGCG1105.636426E-515.99382130
CTGCCGA6200.015.28809718
TGCCGAC6350.015.27409819
TATAAGA2900.015.1492122
GTATAAG2800.014.9057441
CGTCAGA1057.238965E-414.64423752
CTTACCC1201.197627E-414.6415953
Produced by FastQC (version 0.11.5)