FastQCFastQC Report
Wed 25 May 2016
SRR1780309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830989
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT209692.5233787691533824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC64300.7737767888624278No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG49110.5909825521156116No Hit
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG35790.4306916216700823Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC11860.1427215041354338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT29050.032.64411544
CGTCTTC5450.029.4713642
TATGCCG34800.027.44000843
CCGTCTT6100.026.69166441
GTCTTCT6350.025.9873143
TCTTCTG6650.024.48408344
GCCGTCT6850.023.44801140
CTCGTAT42750.022.38590639
TCTCGTA43050.022.28100838
TCGTATG42800.022.25962840
CGTATGC42900.022.2590341
GTATCTC42900.022.10117335
TTGTATC42700.022.05013533
ATCTCGT43500.022.04918737
ACCCGTT43250.021.97318527
TATCTCG43300.021.94781136
GTATGCC43550.021.92680442
CCGTTTG43150.021.92082429
GACCCGT43550.021.87233426
ACGAGAC44000.021.79994222
Produced by FastQC (version 0.11.5)