FastQCFastQC Report
Wed 25 May 2016
SRR1780308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147216
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAT150.002221041870.0000116
GATGTAT200.006940060852.524
ACCCTTG200.006940060852.562
CTTTCGC200.006940060852.542
TTGCATG200.006940060852.514
TTGCAAC200.006940060852.530
CTGATTA200.006940060852.541
GGATTGC352.078531E-550.0000046
GATTACT305.86262E-446.66666843
TGATTAC350.001252205340.00000442
TAGATCT753.506466E-837.33333239
GTTTAAC501.7032622E-435.033
TAGCCCG400.002412609835.05
GCCGTAT400.002412609835.054
AGATCTC904.452886E-934.99999640
TATAGAG450.00429638531.1111097
GATTCTA450.00429638531.11110933
CACCCGT604.9634586E-429.1666686
TGCCATC500.007190282527.99999852
CGCCTCA500.007190282527.99999864