##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780308_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147216 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.10966878600152 34.0 31.0 34.0 12.0 34.0 2 30.208149929355503 34.0 31.0 34.0 12.0 34.0 3 29.990469785892838 34.0 31.0 34.0 11.0 34.0 4 29.94371535702641 34.0 31.0 34.0 11.0 34.0 5 29.927833931094447 34.0 31.0 34.0 11.0 34.0 6 32.62724839691338 37.0 32.0 38.0 11.0 38.0 7 33.1080317356809 38.0 34.0 38.0 12.0 38.0 8 32.96748994674492 38.0 34.0 38.0 11.0 38.0 9 33.36552412781219 38.0 34.0 38.0 12.0 38.0 10-11 33.11145188023041 38.0 34.0 38.0 11.0 38.0 12-13 33.227505841756326 38.0 34.0 38.0 11.0 38.0 14-15 33.12956811759591 38.0 34.0 38.0 11.0 38.0 16-17 33.19790308118682 38.0 34.0 38.0 11.0 38.0 18-19 33.207365367894795 38.0 34.0 38.0 11.0 38.0 20-21 33.212402184545155 38.0 34.0 38.0 11.0 38.0 22-23 33.209352244321266 38.0 34.0 38.0 11.0 38.0 24-25 33.21645745027715 38.0 34.0 38.0 11.0 38.0 26-27 33.17483154004999 38.0 34.0 38.0 11.0 38.0 28-29 33.267301108575154 38.0 34.0 38.0 11.0 38.0 30-31 33.281677942614934 38.0 34.0 38.0 11.0 38.0 32-33 33.34275486360178 38.0 34.0 38.0 11.0 38.0 34-35 33.29046774807087 38.0 34.0 38.0 11.0 38.0 36-37 33.23009727203565 38.0 34.0 38.0 11.0 38.0 38-39 33.20629551135746 38.0 34.0 38.0 11.0 38.0 40-41 33.28186813933268 38.0 34.0 38.0 11.0 38.0 42-43 33.34394019671775 38.0 34.0 38.0 11.0 38.0 44-45 33.43479988588197 38.0 34.0 38.0 11.0 38.0 46-47 33.347000326051514 38.0 34.0 38.0 11.0 38.0 48-49 33.28921788392566 38.0 34.0 38.0 11.0 38.0 50-51 33.22781830779263 38.0 34.0 38.0 11.0 38.0 52-53 33.26917930116292 38.0 34.0 38.0 11.0 38.0 54-55 33.27900499945658 38.0 34.0 38.0 11.0 38.0 56-57 33.21481700358657 38.0 34.0 38.0 11.0 38.0 58-59 33.25850111400935 38.0 34.0 38.0 11.0 38.0 60-61 33.279307276383 38.0 34.0 38.0 11.0 38.0 62-63 33.14638014889685 38.0 34.0 38.0 11.0 38.0 64-65 33.09022456798174 38.0 34.0 38.0 11.0 38.0 66-67 32.987216063471365 38.0 33.5 38.0 11.0 38.0 68-69 32.91227855667862 38.0 33.0 38.0 11.0 38.0 70-71 32.86265759156613 38.0 33.0 38.0 11.0 38.0 72-73 32.787519698945765 38.0 33.0 38.0 11.0 38.0 74-75 32.774851238995765 38.0 32.0 38.0 11.0 38.0 76 31.163874850559722 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1756308767700041 1101 2 0.45595710450783145 1101 3 -0.033594310095576674 1101 4 0.34761242784777124 1101 5 -0.11547701418038514 1101 6 0.24036972115055733 1101 7 -0.2187138284156518 1101 8 0.37368924045686214 1101 9 -0.29000645438319594 1101 10-11 0.17544523353893027 1101 12-13 0.4977078937485757 1101 14-15 0.33448778761734843 1101 16-17 0.036459328839363536 1101 18-19 0.21201079218287333 1101 20-21 0.11772844501656721 1101 22-23 -0.14962936148403827 1101 24-25 -0.10454756252023145 1101 26-27 -0.06806904423367399 1101 28-29 0.11122704093798319 1101 30-31 0.15151549818546783 1101 32-33 0.06064429172953112 1101 34-35 0.0842343208969254 1101 36-37 -0.12904756479875346 1101 38-39 0.15299776303658774 1101 40-41 0.16379733550807885 1101 42-43 0.27138767085582316 1101 44-45 0.2977204003230085 1101 46-47 0.358523676259189 1101 48-49 0.4458095775362523 1101 50-51 0.16258073122380523 1101 52-53 0.16673490545104386 1101 54-55 0.12056453298595926 1101 56-57 0.4871456238191456 1101 58-59 0.44794603552507795 1101 60-61 0.27256932781376975 1101 62-63 0.04651580223652019 1101 64-65 0.4090548119121351 1101 66-67 0.26733427922016517 1101 68-69 0.24437433212770543 1101 70-71 0.27739609733345105 1101 72-73 0.5719576440625076 1101 74-75 0.22329551216363086 1101 76 -0.17402898700300184 1102 1 -0.048403169268194546 1102 2 -0.3252444515482118 1102 3 0.07617783884121465 1102 4 0.38486125056853027 1102 5 -0.9730189136217255 1102 6 0.32017609162058847 1102 7 -0.9132793319401742 1102 8 -0.26097395530786116 1102 9 -0.07327007718585321 1102 10-11 -0.1038112524653414 1102 12-13 0.19979774721021926 1102 14-15 -0.4788512980534847 1102 16-17 -0.01415636626725103 1102 18-19 -0.39380540600952685 1102 20-21 0.06325917127355041 1102 22-23 -0.40819205708788786 1102 24-25 -0.27145797230115676 1102 26-27 -0.45739748320526985 1102 28-29 -0.3032922488920704 1102 30-31 -0.02193210191983752 1102 32-33 0.06659649857968475 1102 34-35 -0.09678150290604748 1102 36-37 -0.5790022285795047 1102 38-39 -0.21398430721992412 1102 40-41 -0.0937050775487478 1102 42-43 -0.26635838781801624 1102 44-45 -0.2240994832652774 1102 46-47 -0.5765577826895978 1102 48-49 -0.46473913696255664 1102 50-51 -0.3292660616042937 1102 52-53 -0.3264281001940432 1102 54-55 -0.4382116815859831 1102 56-57 -0.269567061547491 1102 58-59 -0.6904391175943516 1102 60-61 -0.2774613838186326 1102 62-63 -0.3528524156113235 1102 64-65 -0.14738878105636388 1102 66-67 -0.2651103667185879 1102 68-69 -0.0013114147499493356 1102 70-71 -0.5523152133220179 1102 72-73 0.12120594029813958 1102 74-75 -0.39416185716569174 1102 76 -0.001765978432992199 1103 1 -0.11608823786120936 1103 2 0.297043307746641 1103 3 0.1737389760935173 1103 4 0.230046484845154 1103 5 0.5358418458719463 1103 6 -0.9055181227077291 1103 7 -0.07293205909240896 1103 8 -0.002189410275050818 1103 9 -0.2845895814604944 1103 10-11 -0.25071628261975576 1103 12-13 0.23387223856152417 1103 14-15 0.003450378664652476 1103 16-17 0.307935886367126 1103 18-19 0.19986879373863076 1103 20-21 0.07148701937481405 1103 22-23 0.07900722715891817 1103 24-25 0.3224448708295995 1103 26-27 0.219244441375551 1103 28-29 -0.5036269297854474 1103 30-31 -0.32142842374367575 1103 32-33 -0.045776753458227404 1103 34-35 -0.42546796324138825 1103 36-37 0.047043235003236816 1103 38-39 -0.5594942813732828 1103 40-41 -0.21416037611061256 1103 42-43 -0.3432223284370082 1103 44-45 -0.07004354847834549 1103 46-47 0.07989062343406772 1103 48-49 0.2389324118478342 1103 50-51 -0.5259490757205398 1103 52-53 -0.42628892596115264 1103 54-55 -0.24164464599714108 1103 56-57 -0.24849541995428126 1103 58-59 -0.17678347441056985 1103 60-61 -0.21162203064744745 1103 62-63 -0.33963086348725824 1103 64-65 0.027907792606569615 1103 66-67 -0.05682949986194075 1103 68-69 0.009772818797671334 1103 70-71 -0.19487601920710063 1103 72-73 -0.22914199528407408 1103 74-75 0.009198206456822788 1103 76 -0.1835474086139186 1104 1 -0.058173648933962596 1104 2 0.058448498898666656 1104 3 0.1530063332089746 1104 4 0.37609558342590077 1104 5 -0.5285744691772827 1104 6 0.2618837440612225 1104 7 0.23748221018261972 1104 8 0.31363337453507256 1104 9 0.1445627008563477 1104 10-11 0.0096532353446932 1104 12-13 0.3487744249827003 1104 14-15 0.16472451240714747 1104 16-17 0.5123918382914283 1104 18-19 0.5006231729740236 1104 20-21 0.13270361571799327 1104 22-23 0.2500659153062159 1104 24-25 0.37389609190108075 1104 26-27 0.26984219102392615 1104 28-29 0.3695222838701042 1104 30-31 0.5511613985296933 1104 32-33 0.6680098467207785 1104 34-35 0.48400013546721254 1104 36-37 0.5791235316404908 1104 38-39 0.681184659938566 1104 40-41 0.5658485741515022 1104 42-43 0.48120229756989374 1104 44-45 0.500925637047807 1104 46-47 0.5673155051627461 1104 48-49 0.6378839525715208 1104 50-51 0.5114182686088284 1104 52-53 0.30522878277174215 1104 54-55 0.21029564296846104 1104 56-57 0.8045444726425757 1104 58-59 0.5199368938287847 1104 60-61 0.4219542383718604 1104 62-63 0.8199555065421436 1104 64-65 0.43553469453726734 1104 66-67 0.48892871520007475 1104 68-69 0.6009216488705533 1104 70-71 0.5913597562691848 1104 72-73 0.5761546419745827 1104 74-75 0.5185384442780716 1104 76 0.4074703640494235 1105 1 -0.21206177644017998 1105 2 -0.4704205940407782 1105 3 -0.3077443901045598 1105 4 -0.057626055069608384 1105 5 0.20788560899134012 1105 6 -0.04905224903556871 1105 7 -0.20415780809056372 1105 8 -0.7831002938066334 1105 9 -0.20963251766048074 1105 10-11 -0.22730174156003358 1105 12-13 -1.301294099457678 1105 14-15 -0.10184190554118544 1105 16-17 -0.4988639355271616 1105 18-19 -0.1800402451265768 1105 20-21 -0.45684133123901205 1105 22-23 -0.6528577941501581 1105 24-25 -0.3455851527057945 1105 26-27 -0.28047885866898525 1105 28-29 -0.5983473674166291 1105 30-31 -0.27962764771162085 1105 32-33 -0.6287453941299361 1105 34-35 -0.3678094173473596 1105 36-37 -0.5468979901096702 1105 38-39 -0.6664626676082577 1105 40-41 -0.27282712758952954 1105 42-43 -0.43765334427569513 1105 44-45 -0.439864170576179 1105 46-47 -0.565685357737614 1105 48-49 -0.9282675118211472 1105 50-51 -0.32018926400373005 1105 52-53 -0.46763807677853464 1105 54-55 -0.3876118236575934 1105 56-57 -0.6542456902933083 1105 58-59 -0.5192362445905871 1105 60-61 -0.6860225140772513 1105 62-63 -0.4373981810879215 1105 64-65 -0.6661606508766695 1105 66-67 -0.7644657825411585 1105 68-69 -1.0347377085224778 1105 70-71 -0.5842023979361883 1105 72-73 -0.5565480127129945 1105 74-75 -0.4377962595440721 1105 76 -0.43196672036253503 1106 1 -0.28140081858970234 1106 2 -0.10741936339776714 1106 3 -0.26342842251149534 1106 4 0.24661197179943173 1106 5 0.14177700474562016 1106 6 0.5106211177985998 1106 7 -0.33765693638509475 1106 8 -0.20422376832207334 1106 9 0.4556325833547987 1106 10-11 0.18852099456959337 1106 12-13 0.2606482654279745 1106 14-15 0.27234407538384886 1106 16-17 0.32478876675978796 1106 18-19 -0.33532560047474647 1106 20-21 0.4535652937225265 1106 22-23 0.061870182467622215 1106 24-25 0.1180467824803415 1106 26-27 -0.26062833051801704 1106 28-29 0.44841065740133246 1106 30-31 -0.14320574226602645 1106 32-33 -0.034242266245612996 1106 34-35 0.027470553937632758 1106 36-37 0.33247335641888753 1106 38-39 0.08865219240609434 1106 40-41 0.428312499115421 1106 42-43 0.2029091621338992 1106 44-45 0.14217228722302622 1106 46-47 0.5108580737053146 1106 48-49 0.21226294659337697 1106 50-51 0.106978462383303 1106 52-53 -0.10624565548841503 1106 54-55 0.06599549866832177 1106 56-57 0.28718980350219425 1106 58-59 -0.13903805978902994 1106 60-61 0.0327947867124152 1106 62-63 -0.1957242502090395 1106 64-65 0.12545687553087959 1106 66-67 0.13612774632767355 1106 68-69 0.4006278950053712 1106 70-71 -0.22561097141583275 1106 72-73 0.1326120734320213 1106 74-75 0.09441197550874136 1106 76 0.6094905660696242 1107 1 -0.4448102855706004 1107 2 -0.6312184407682722 1107 3 -0.15186672166407789 1107 4 -0.5536750963447794 1107 5 -0.07867001927283113 1107 6 -0.2654845333070526 1107 7 -0.3626406399402242 1107 8 0.20613951704121547 1107 9 0.0970606533542977 1107 10-11 -0.008023532332067873 1107 12-13 0.09386042006870099 1107 14-15 -0.22658725390462564 1107 16-17 0.06241913831872381 1107 18-19 -0.21122503887418986 1107 20-21 0.25920511721950135 1107 22-23 0.3919578071981036 1107 24-25 -0.05471433670935255 1107 26-27 0.42548647166820786 1107 28-29 0.29171720606502305 1107 30-31 -0.2144337420654523 1107 32-33 0.47243927615021164 1107 34-35 0.14404654551361773 1107 36-37 0.20538615790312065 1107 38-39 0.05772028647419347 1107 40-41 0.26766402596695116 1107 42-43 0.1731556145232105 1107 44-45 0.2082010943232575 1107 46-47 0.13208481993206078 1107 48-49 -0.05144173675416397 1107 50-51 -0.01963551244495676 1107 52-53 0.20558368312664044 1107 54-55 0.16651322418604764 1107 56-57 0.04522833832643869 1107 58-59 -0.21163558774370017 1107 60-61 -0.053748737331112295 1107 62-63 0.09244390403054581 1107 64-65 0.08083908984166754 1107 66-67 0.06219516346651943 1107 68-69 0.11945154240044076 1107 70-71 -0.13297821806879284 1107 72-73 -0.060174869354924 1107 74-75 -0.01811184237221397 1107 76 -0.30267159609253724 1108 1 -0.43631908312473655 1108 2 -0.14871762073596528 1108 3 0.031622469381787255 1108 4 0.08564065632109674 1108 5 0.24108687982915455 1108 6 -0.11563832804522889 1108 7 -0.07835245892660225 1108 8 -0.7927897992684549 1108 9 -0.39570140112059704 1108 10-11 -0.2955206699551596 1108 12-13 -0.5028972031372874 1108 14-15 0.3680073406899709 1108 16-17 -0.1267514841012698 1108 18-19 -0.05656075700587593 1108 20-21 -0.2874964962327482 1108 22-23 0.29935604583059927 1108 24-25 -0.20337536320413108 1108 26-27 -0.2188577809883867 1108 28-29 -0.032508095490250355 1108 30-31 0.1322016499099945 1108 32-33 -0.03867602967542183 1108 34-35 0.14959182058031217 1108 36-37 -0.4695623193123595 1108 38-39 -0.1889206755320103 1108 40-41 -0.24371430908897196 1108 42-43 0.001725540070466991 1108 44-45 -0.1841302111062859 1108 46-47 0.3142495814419135 1108 48-49 0.23759644271222413 1108 50-51 -0.26248575907353455 1108 52-53 0.1766380899493072 1108 54-55 -0.007547142569535481 1108 56-57 -0.1691312009701278 1108 58-59 0.11113222242965293 1108 60-61 -0.2524472068104444 1108 62-63 -0.05778601446134246 1108 64-65 0.23405516371264667 1108 66-67 -0.2325461086047227 1108 68-69 -0.14088241188817108 1108 70-71 0.07766487292694535 1108 72-73 -0.34804643682346637 1108 74-75 0.35906588493146074 1108 76 -0.5435321039555632 1109 1 0.18855529857218656 1109 2 -0.08805540019590552 1109 3 0.6164621347476782 1109 4 -0.23502861189518853 1109 5 0.43889818482387 1109 6 0.33121697272088113 1109 7 0.7008595064614767 1109 8 -0.0762629985219192 1109 9 0.8073826097902455 1109 10-11 0.8102760437395276 1109 12-13 0.39575982300437573 1109 14-15 1.0765565634878982 1109 16-17 0.543218236993475 1109 18-19 0.73513146836315 1109 20-21 0.5360570987346946 1109 22-23 0.9568487697574639 1109 24-25 0.44065173119557954 1109 26-27 0.7753833539485129 1109 28-29 0.5885912114882501 1109 30-31 0.529840678374768 1109 32-33 0.3378101816765593 1109 34-35 0.3819302134491096 1109 36-37 0.5422914805597401 1109 38-39 0.7804545610219407 1109 40-41 0.36886579297182465 1109 42-43 0.6120901920588224 1109 44-45 0.7410078058864471 1109 46-47 0.23744948747289385 1109 48-49 0.2613073052694901 1109 50-51 0.644576273528493 1109 52-53 0.4425475664635812 1109 54-55 0.5039118569214693 1109 56-57 0.2306906494364256 1109 58-59 0.69987094941051 1109 60-61 0.6534924180965689 1109 62-63 0.566416227743801 1109 64-65 0.5232904908422924 1109 66-67 0.7266360999281858 1109 68-69 0.8728442426843372 1109 70-71 0.6531957326129287 1109 72-73 0.8691035612188429 1109 74-75 0.5136318655372989 1109 76 0.32581180959294187 1110 1 -0.0930785353616912 1110 2 -0.38954668133001746 1110 3 0.14635351056630697 1110 4 0.43350982738836663 1110 5 -0.24459251768264068 1110 6 -0.2641250750948032 1110 7 -0.5600489388713683 1110 8 -0.20540215845985443 1110 9 -0.03571725607335452 1110 10-11 -0.3766206712337805 1110 12-13 -0.39430356301914316 1110 14-15 -0.554581491797336 1110 16-17 -0.17928380135375477 1110 18-19 -0.45266606457917646 1110 20-21 0.06325917127355041 1110 22-23 -0.3340365304713586 1110 24-25 -0.40947056963818085 1110 26-27 -0.2719654795908468 1110 28-29 -0.560142383104214 1110 30-31 -0.2568232704955946 1110 32-33 -0.17308511302900342 1110 34-35 -0.1783851385931854 1110 36-37 -0.5968750326829451 1110 38-39 -0.42829849649890406 1110 40-41 -0.37122520936898695 1110 42-43 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-0.1756193940954489 2116 8 -0.1021420265344446 2116 9 -0.6130130567194101 2116 10-11 -0.4612320884818004 2116 12-13 -0.4252897461402867 2116 14-15 -0.15873062722447173 2116 16-17 -0.03633068101932935 2116 18-19 -0.15995317877879955 2116 20-21 -0.40331836883340344 2116 22-23 0.2607611024719816 2116 24-25 -0.4312138308559028 2116 26-27 0.09953618983557533 2116 28-29 -0.38092334892846935 2116 30-31 -0.2237197660573571 2116 32-33 -0.07077506118177723 2116 34-35 0.3589258633341146 2116 36-37 -0.37215394904875154 2116 38-39 -0.030045286585497877 2116 40-41 -0.5752684787302655 2116 42-43 -0.23203299654775122 2116 44-45 -0.14405356675493408 2116 46-47 -0.248786693292395 2116 48-49 -0.39256762199769213 2116 50-51 0.08702730010021043 2116 52-53 -0.09554364741244825 2116 54-55 -0.36687310331792844 2116 56-57 -0.4545909997281896 2116 58-59 -0.13781711686640108 2116 60-61 -0.6830665578254127 2116 62-63 -0.06856202168125947 2116 64-65 -0.33659840112523653 2116 66-67 -0.491389169529576 2116 68-69 -0.5944883333041417 2116 70-71 -0.11823620332677365 2116 72-73 -0.5562725713349721 2116 74-75 -0.23190718852050196 2116 76 -0.16189980826192496 2117 1 -0.07774435600466845 2117 2 -0.6292093293841603 2117 3 -0.1796167728516309 2117 4 -0.4397188105134937 2117 5 0.3134394197116066 2117 6 0.5627044511319355 2117 7 0.012576907152322292 2117 8 -0.25147079213159174 2117 9 -0.538202450658801 2117 10-11 -0.005064252029036709 2117 12-13 -0.11902860532033088 2117 14-15 -0.2517843259766934 2117 16-17 -0.263965484940897 2117 18-19 -0.12848036237950566 2117 20-21 -0.6398658287264496 2117 22-23 -0.21951268317864248 2117 24-25 -0.6518299181999367 2117 26-27 -0.6828487811982882 2117 28-29 -0.5412258221905049 2117 30-31 -0.1170046368238431 2117 32-33 -0.4461789159054774 2117 34-35 -0.25787170796712644 2117 36-37 -0.204888463309004 2117 38-39 -0.36131060521294245 2117 40-41 -0.21603273897981978 2117 42-43 -0.21221626303616858 2117 44-45 -0.14072524679058773 2117 46-47 0.04806045465770126 2117 48-49 -0.20854912823655525 2117 50-51 -0.004522387956825469 2117 52-53 -0.2882450177333098 2117 54-55 -0.32407146561739353 2117 56-57 -0.640531284933175 2117 58-59 -0.6765593182924263 2117 60-61 -0.349273665668548 2117 62-63 -0.25670958184169024 2117 64-65 -0.6182452928364697 2117 66-67 -0.2347534186383058 2117 68-69 -0.25336338900823563 2117 70-71 -0.09811309726617878 2117 72-73 -1.5764653075203583 2117 74-75 -0.7269634496613193 2117 76 -1.0780094339303758 2118 1 0.4601942404865582 2118 2 0.06613058289654106 2118 3 0.7899281832887226 2118 4 0.14970004913562818 2118 5 0.392770560062484 2118 6 -0.05122098746456061 2118 7 0.3458554159242482 2118 8 0.6899491640087589 2118 9 -0.09818052083424078 2118 10-11 -0.09701874764307661 2118 12-13 0.08645385082002832 2118 14-15 0.04037576174260238 2118 16-17 0.2540717957608578 2118 18-19 0.2170240235854024 2118 20-21 0.06062759232617765 2118 22-23 -0.05148910861723266 2118 24-25 0.5655494677649742 2118 26-27 0.2388480389771459 2118 28-29 -0.11022253271681848 2118 30-31 -0.28638511927999133 2118 32-33 0.05055902269100443 2118 34-35 0.10416228326093346 2118 36-37 0.19952491388398386 2118 38-39 0.06515595180459144 2118 40-41 -0.04772133547103863 2118 42-43 0.38419272819190553 2118 44-45 0.2508591830339739 2118 46-47 0.39751220904366136 2118 48-49 -0.005204829990944404 2118 50-51 0.3202034892361567 2118 52-53 -0.01632889931225634 2118 54-55 0.26486767911944753 2118 56-57 0.06714415366330684 2118 58-59 -0.009728177941553895 2118 60-61 0.054317343103384985 2118 62-63 -0.014480963420638204 2118 64-65 0.004739904531952277 2118 66-67 0.25158155943186955 2118 68-69 0.2099726855531685 2118 70-71 0.07685949045312412 2118 72-73 0.24201056967262247 2118 74-75 0.41319554156674343 2118 76 -0.0273515391935355 2119 1 0.7519514111910972 2119 2 0.4272556177057858 2119 3 0.34456477476741654 2119 4 0.5469710043013194 2119 5 0.7042297636269517 2119 6 0.26657349875097935 2119 7 0.28498100768142365 2119 8 0.7514640623657627 2119 9 0.043312099605749665 2119 10-11 0.4852424609339252 2119 12-13 0.4758788020870881 2119 14-15 0.4023988750815022 2119 16-17 0.6150854409850339 2119 18-19 0.10944953180037942 2119 20-21 0.3026771606915375 2119 22-23 0.5497292149695099 2119 24-25 0.37834534635032924 2119 26-27 0.14484219102392615 2119 28-29 0.534625698973521 2119 30-31 0.24133696370525115 2119 32-33 0.5793253830363199 2119 34-35 0.24638589785297427 2119 36-37 0.16221636473332524 2119 38-39 0.11047758923150042 2119 40-41 0.8122410705439975 2119 42-43 0.15935285072044536 2119 44-45 0.3393094754316408 2119 46-47 0.24035542820267608 2119 48-49 0.4089277236152995 2119 50-51 0.41173091892147795 2119 52-53 0.290438017980982 2119 54-55 0.25184205951488536 2119 56-57 0.47091051400862227 2119 58-59 0.2668103907022754 2119 60-61 0.4835222999399207 2119 62-63 0.401545213131854 2119 64-65 0.13863714763972013 2119 66-67 0.7181855694569279 2119 68-69 -0.3503169273680271 2119 70-71 0.1690413339507586 2119 72-73 0.43684487766482505 2119 74-75 -0.12275544701582675 2119 76 -0.2833665767875182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 28.0 13 367.0 14 1268.0 15 2242.0 16 3067.0 17 3425.0 18 3658.0 19 3213.0 20 2544.0 21 1819.0 22 1356.0 23 1188.0 24 1036.0 25 1158.0 26 1210.0 27 1381.0 28 1653.0 29 1912.0 30 2386.0 31 2912.0 32 3832.0 33 4765.0 34 6811.0 35 10133.0 36 19056.0 37 64795.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.63609933702858 16.9818497989349 10.157863275730898 31.224187588305618 2 21.931040104336486 21.158705575480926 28.54716878600152 28.363085534181064 3 19.445576567764373 19.33213781110749 26.827926312357352 34.39435930877078 4 15.331893272470385 17.344582110640147 30.193728942506247 37.12979567438322 5 16.022714922291055 30.508232800782526 31.989050103249646 21.480002173676773 6 30.12580154331051 29.69717965438539 19.716606890555376 20.460411911748725 7 31.05980328225193 24.876372133463754 19.100505379850016 24.9633192044343 8 25.714596239539176 28.81344419084882 20.968508857732854 24.503450711879143 9 24.521111835670037 16.688403434409306 19.414329964134332 39.376154765786325 10-11 26.37451092272579 21.762240517335073 24.54624497337246 27.317003586566678 12-13 25.47786925334203 19.492106836213456 24.279290294533205 30.75073361591131 14-15 24.857521312366266 20.554291342594162 22.19848520870835 32.389702136331216 16-17 25.606252037821974 22.946554722312793 22.742093250733618 28.705099989131615 18-19 24.827124769046844 22.029874470166284 23.36023258341485 29.782768177372027 20-21 24.63455059232692 22.249619606564504 23.85983860449951 29.255991196609067 22-23 26.230165199434847 22.032931203130094 22.776736224323443 28.960167373111616 24-25 25.279860884686446 23.036898163243123 22.312112813824584 29.371128138245844 26-27 24.334311487881752 23.369063145310292 23.66930224975546 28.627323117052494 28-29 25.103589283773502 22.64427779589175 24.803010542332355 27.44912237800239 30-31 25.08524888599065 22.23773231170525 24.524847842625803 28.152170959678298 32-33 23.973277361156395 23.328306705792848 24.559151179219647 28.139264753831107 34-35 24.375747201391153 22.870476035213564 24.784330507553527 27.969446255841756 36-37 24.83018150201065 21.83050755352679 24.9585642864906 28.38074665797196 38-39 23.634659276165635 21.906246603630038 26.707015541788937 27.75207857841539 40-41 23.616318878382785 21.379469622867077 26.9355912400826 28.068620258667536 42-43 22.3596619932616 21.39950820562982 26.970234213672427 29.270595587436148 44-45 21.591403108357788 21.135610259754376 28.56550918378437 28.707477448103468 46-47 21.75476850342354 21.748315400499944 28.290403217041625 28.20651287903489 48-49 21.32207097054668 22.2241468318661 28.006466688403435 28.447315509183785 50-51 21.02964351700902 23.195169003369198 27.092163895228776 28.683023584393002 52-53 20.202287794804914 23.375855885229864 27.172657863275727 29.24919845668949 54-55 20.245082056298227 22.90104336485165 27.0344256059124 29.819448972937725 56-57 20.528678947940442 23.13810998804478 26.817397565482015 29.51581349853277 58-59 20.97360341267254 23.097013911531356 26.051855776546027 29.877526899250086 60-61 20.741291707423105 22.07063090968373 26.798038256711227 30.39003912618194 62-63 21.173989240299967 22.398720247799154 26.135746114552767 30.29154439734811 64-65 21.91507716552549 22.45679817411151 25.175931963916966 30.45219269644604 66-67 22.003722421475928 23.282116074339747 23.759985327681772 30.95417617650255 68-69 21.892321486794913 23.835724377785024 23.679151722638846 30.592802412781218 70-71 23.04878545810238 23.39555483099663 24.458278991413977 29.097380719487013 72-73 24.15328496902511 23.112637213346375 24.138340941202046 28.595736876426475 74-75 23.628545810238016 24.51703619171829 23.902972502988806 27.951445495054884 76 23.316759047929573 24.182154113683296 24.190305401586784 28.310781436800347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 3.0 15 7.5 16 12.0 17 14.0 18 26.0 19 36.5 20 34.5 21 34.0 22 49.5 23 89.5 24 130.5 25 147.0 26 188.0 27 314.5 28 471.5 29 543.0 30 656.5 31 901.5 32 1277.5 33 1522.0 34 1716.5 35 2203.5 36 2765.0 37 3034.0 38 3393.5 39 4299.0 40 5246.0 41 5956.0 42 6265.0 43 6434.0 44 7017.5 45 7426.0 46 7420.0 47 7460.0 48 7191.0 49 6641.0 50 6400.0 51 6055.0 52 5327.0 53 4575.0 54 4206.0 55 4127.0 56 3844.5 57 3518.0 58 3395.0 59 3307.0 60 3303.5 61 3407.5 62 3427.0 63 3531.0 64 3581.0 65 3524.5 66 3595.0 67 3668.0 68 3632.0 69 3578.5 70 3435.0 71 3309.0 72 3138.5 73 2763.5 74 2302.0 75 2045.0 76 1818.0 77 1284.0 78 768.0 79 559.0 80 414.5 81 197.5 82 85.0 83 45.0 84 32.5 85 14.0 86 8.5 87 9.0 88 8.5 89 9.0 90 9.0 91 8.5 92 9.0 93 8.5 94 6.5 95 4.5 96 4.0 97 5.5 98 6.5 99 18.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.79273991957396E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.99633192044342 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82797721690964 49.285403760460824 2 13.705387469300309 17.81599826105858 3 5.022730835554162 9.793772416041735 4 2.040027172493076 5.3037713292033475 5 1.030464545122015 3.348820780349962 6 0.5967497517897267 2.3271926964460383 7 0.43162460155719284 1.9637811107488314 8 0.34488164289073525 1.793283338767525 9 0.22887599937294248 1.3388490381480274 >10 0.7712807650101897 7.029127268775133 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006792739919573959 0.0 2 0.0 0.0 0.0 0.007472013911531355 0.0 3 0.0 0.0 0.0 0.008830561895446147 0.0 4 0.0 0.0 0.0 0.024453863710466255 0.0 5 0.0 0.0 0.0 0.027850233670253233 0.0 6 0.0 0.0 0.0 0.027850233670253233 0.0 7 0.0 0.0 0.0 0.027850233670253233 6.79273991957396E-4 8 0.0 0.0 0.0 0.02852950766221063 6.79273991957396E-4 9 0.0 0.0 0.0 0.033963699597869795 6.79273991957396E-4 10 0.0 0.0 0.0 0.03803934354961417 6.79273991957396E-4 11 0.0 0.0 0.0 0.04075643951744375 6.79273991957396E-4 12 0.0 0.0 0.0 0.042114987501358545 6.79273991957396E-4 13 0.0 0.0 0.0 0.04551135746114553 6.79273991957396E-4 14 0.0 0.0 0.0 0.046190631453102926 6.79273991957396E-4 15 0.0 0.0 0.0 0.04822845342897511 6.79273991957396E-4 16 0.0 0.0 0.0 0.05094554939680469 6.79273991957396E-4 17 0.0 0.0 0.0 0.052304097380719485 6.79273991957396E-4 18 0.0 0.0 0.0 0.05773828931637866 6.79273991957396E-4 19 0.0 0.0 0.0 0.06113465927616563 6.79273991957396E-4 20 0.0 0.0 0.0 0.06453102923595261 6.79273991957396E-4 21 0.0 0.0 0.0 0.06792739919573959 6.79273991957396E-4 22 0.0 0.0 0.0 0.07404086512335616 6.79273991957396E-4 23 0.0 0.0 0.0 0.08422997500271709 6.79273991957396E-4 24 0.0 0.0 0.0 0.08898489294641887 6.79273991957396E-4 25 0.0 0.0 0.0 0.09577763286599282 6.79273991957396E-4 26 0.0 0.0 0.0 0.11955222258450168 6.79273991957396E-4 27 0.0 0.0 0.0 0.20717856754700575 6.79273991957396E-4 28 0.0 0.0 0.0 0.30635257037278557 6.79273991957396E-4 29 0.0 0.0 0.0 0.4204706010216281 6.79273991957396E-4 30 0.0 0.0 0.0 0.5474948375176611 6.79273991957396E-4 31 0.0 0.0 0.0 0.662292142158461 6.79273991957396E-4 32 0.0 0.001358547983914792 0.0 0.8171666123247473 6.79273991957396E-4 33 0.0 0.001358547983914792 0.0 1.0182317139441366 6.79273991957396E-4 34 0.0 0.001358547983914792 0.0 1.2906205847190522 6.79273991957396E-4 35 0.0 0.001358547983914792 0.0 1.6879958700141289 6.79273991957396E-4 36 0.0 0.001358547983914792 0.0 2.2334528855559177 6.79273991957396E-4 37 0.0 0.001358547983914792 0.0 2.974540810781437 6.79273991957396E-4 38 0.0 0.001358547983914792 0.0 3.757743723508314 6.79273991957396E-4 39 0.0 0.001358547983914792 0.0 4.540946636235192 6.79273991957396E-4 40 0.0 0.001358547983914792 0.0 5.377812194326704 6.79273991957396E-4 41 0.0 0.001358547983914792 0.0 6.317248125203782 6.79273991957396E-4 42 0.0 0.001358547983914792 0.0 7.323932181284643 6.79273991957396E-4 43 0.0 0.001358547983914792 0.0 8.402619280512988 6.79273991957396E-4 44 0.0 0.001358547983914792 0.0 9.401831322682318 6.79273991957396E-4 45 0.0 0.001358547983914792 0.0 10.373872405173351 6.79273991957396E-4 46 0.0 0.001358547983914792 0.0 11.333007281817194 6.79273991957396E-4 47 0.0 0.001358547983914792 0.0 12.243234431040104 6.79273991957396E-4 48 0.0 0.001358547983914792 0.0 13.099119660906423 6.79273991957396E-4 49 0.0 0.001358547983914792 0.0 13.896587327464406 6.79273991957396E-4 50 0.0 0.001358547983914792 0.0 14.55820019563091 6.79273991957396E-4 51 0.0 0.001358547983914792 0.0 15.263286599282686 6.79273991957396E-4 52 0.0 0.001358547983914792 0.0 16.096076513422453 6.79273991957396E-4 53 0.0 0.001358547983914792 0.0 16.983208346918815 6.79273991957396E-4 54 0.0 0.001358547983914792 0.0 17.892756222149767 6.79273991957396E-4 55 0.0 0.001358547983914792 0.0 18.658977285077707 6.79273991957396E-4 56 0.0 0.001358547983914792 0.0 19.385800456472122 6.79273991957396E-4 57 0.0 0.001358547983914792 0.0 20.081377024236495 6.79273991957396E-4 58 0.0 0.001358547983914792 0.0 20.742989892403 6.79273991957396E-4 59 0.0 0.001358547983914792 0.0 21.362487773068146 6.79273991957396E-4 60 0.0 0.001358547983914792 0.0 21.986061297685033 6.79273991957396E-4 61 0.0 0.001358547983914792 0.0 22.59265297250299 6.79273991957396E-4 62 0.0 0.001358547983914792 0.0 23.209433757200305 6.79273991957396E-4 63 0.0 0.001358547983914792 0.0 23.905010324964678 6.79273991957396E-4 64 0.0 0.001358547983914792 0.0 24.614851646560158 6.79273991957396E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCAAT 15 0.0022210418 70.00001 16 GATGTAT 20 0.0069400608 52.5 24 ACCCTTG 20 0.0069400608 52.5 62 CTTTCGC 20 0.0069400608 52.5 42 TTGCATG 20 0.0069400608 52.5 14 TTGCAAC 20 0.0069400608 52.5 30 CTGATTA 20 0.0069400608 52.5 41 GGATTGC 35 2.078531E-5 50.000004 6 GATTACT 30 5.86262E-4 46.666668 43 TGATTAC 35 0.0012522053 40.000004 42 TAGATCT 75 3.506466E-8 37.333332 39 GTTTAAC 50 1.7032622E-4 35.0 33 TAGCCCG 40 0.0024126098 35.0 5 GCCGTAT 40 0.0024126098 35.0 54 AGATCTC 90 4.452886E-9 34.999996 40 TATAGAG 45 0.004296385 31.111109 7 GATTCTA 45 0.004296385 31.111109 33 CACCCGT 60 4.9634586E-4 29.166668 6 TGCCATC 50 0.0071902825 27.999998 52 CGCCTCA 50 0.0071902825 27.999998 64 >>END_MODULE