FastQCFastQC Report
Wed 25 May 2016
SRR1780308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147216
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT106357.224078904466906TruSeq Adapter, Index 5 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC27861.8924573415933053RNA PCR Primer, Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG26701.8136615585262472TruSeq Adapter, Index 5 (95% over 22bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG24111.6377295946092816TruSeq Adapter, Index 5 (96% over 29bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7380.5013042060645582RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3670.2492935550483643TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT3200.2173676774263667No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT1990.1351755243995218No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1710.1161558526247147TruSeq Adapter, Index 5 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1670.11343875665688512No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1640.11140093468101292TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT850.061.76471
CTTTATA900.058.333332
AGACTTT200.006940060852.527
GTACACA200.006940060852.52
TACCAAG200.006940060852.521
ATCGTAT200.006940060852.539
TAACACG200.006940060852.54
ATCCACG200.006940060852.52
AGCGACT200.006940060852.567
CACCGTA200.006940060852.526
TCAACGC200.006940060852.53
AACACCA200.006940060852.55
TGCCCAA305.86262E-446.66666834
TATGCAG305.86262E-446.66666810
TTTTGCC305.86262E-446.66666831
ACGAGTG305.86262E-446.6666685
TTTATAC1200.043.7500043
TAATATG350.001252205340.00000418
ACTGAGC350.001252205340.0000048
CCGTCTT24400.037.43852247
Produced by FastQC (version 0.11.5)