##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780308_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147216 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76844908162156 34.0 34.0 34.0 34.0 34.0 2 33.74872975763504 34.0 34.0 34.0 34.0 34.0 3 33.74971470492338 34.0 34.0 34.0 34.0 34.0 4 33.77851592218237 34.0 34.0 34.0 34.0 34.0 5 33.77084012607325 34.0 34.0 34.0 34.0 34.0 6 37.52611808499076 38.0 38.0 38.0 37.0 38.0 7 37.51622106292794 38.0 38.0 38.0 37.0 38.0 8 37.45515433105097 38.0 38.0 38.0 37.0 38.0 9 37.473175470057605 38.0 38.0 38.0 37.0 38.0 10-11 37.40998600695576 38.0 38.0 38.0 37.0 38.0 12-13 37.38952967068797 38.0 38.0 38.0 37.0 38.0 14-15 37.31532238343658 38.0 38.0 38.0 36.0 38.0 16-17 37.34424247364417 38.0 38.0 38.0 36.5 38.0 18-19 37.29657781762852 38.0 38.0 38.0 36.0 38.0 20-21 37.328890881425934 38.0 38.0 38.0 36.5 38.0 22-23 37.31580806434083 38.0 38.0 38.0 36.0 38.0 24-25 37.31016669383763 38.0 38.0 38.0 36.0 38.0 26-27 37.20088169764156 38.0 38.0 38.0 36.0 38.0 28-29 37.19188471361809 38.0 38.0 38.0 36.0 38.0 30-31 37.20223345288555 38.0 38.0 38.0 36.0 38.0 32-33 37.0745265460276 38.0 38.0 38.0 36.0 38.0 34-35 37.096837300293444 38.0 38.0 38.0 36.0 38.0 36-37 37.11340479295728 38.0 38.0 38.0 36.0 38.0 38-39 36.39630203238778 38.0 37.0 38.0 34.0 38.0 40-41 36.728480599934784 38.0 37.0 38.0 34.5 38.0 42-43 37.05293242582328 38.0 38.0 38.0 36.0 38.0 44-45 37.13479513096402 38.0 38.0 38.0 36.0 38.0 46-47 37.097166748179546 38.0 38.0 38.0 36.0 38.0 48-49 37.120676421041196 38.0 38.0 38.0 36.0 38.0 50-51 36.97601143897403 38.0 38.0 38.0 35.5 38.0 52-53 36.95954583740898 38.0 38.0 38.0 36.0 38.0 54-55 36.935737283990875 38.0 38.0 38.0 35.5 38.0 56-57 36.98159507118791 38.0 38.0 38.0 36.0 38.0 58-59 36.87213006738398 38.0 38.0 38.0 35.0 38.0 60-61 36.86535770568416 38.0 38.0 38.0 35.0 38.0 62-63 36.8490279589175 38.0 38.0 38.0 35.0 38.0 64-65 36.782730817302465 38.0 38.0 38.0 34.5 38.0 66-67 36.4609960873818 38.0 37.5 38.0 34.0 38.0 68-69 36.345274970111944 38.0 37.0 38.0 34.0 38.0 70-71 35.59562139984784 38.0 37.0 38.0 29.5 38.0 72-73 34.33445753179002 38.0 36.5 38.0 22.0 38.0 74-75 33.96208292576894 38.0 36.0 38.0 21.0 38.0 76 33.28780839039235 38.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07810175246508777 1101 2 0.02326622783900234 1101 3 0.09531361405449701 1101 4 0.12555944349217185 1101 5 0.04281601401977042 1101 6 -0.231780893845432 1101 7 -0.07740201002577862 1101 8 0.022155211381104323 1101 9 -0.0254165135566069 1101 10-11 0.1694610523326645 1101 12-13 0.04987911320808536 1101 14-15 0.2790384344167194 1101 16-17 0.14580632187954734 1101 18-19 0.05056258781328893 1101 20-21 -0.09163938671498784 1101 22-23 0.025631303679730877 1101 24-25 0.08337804700977358 1101 26-27 -0.0102371513319639 1101 28-29 0.08160983788111764 1101 30-31 -0.16704654511836026 1101 32-33 -0.04842778082171861 1101 34-35 -0.03032279293778828 1101 36-37 0.09884629048807625 1101 38-39 0.6007316706790746 1101 40-41 0.39582646561412815 1101 42-43 0.13676250237414678 1101 44-45 0.22996093027148845 1101 46-47 0.15761487979959554 1101 48-49 -0.1782926267401308 1101 50-51 0.018363054150917435 1101 52-53 0.03646855429967388 1101 54-55 0.13433449517392404 1101 56-57 0.11426326497497286 1101 58-59 0.3193063177297333 1101 60-61 0.05842652662938974 1101 62-63 0.06425220713217072 1101 64-65 0.07231413591457425 1101 66-67 -0.05011869298122207 1101 68-69 -0.5676620732629516 1101 70-71 -0.16876432187277857 1101 72-73 0.4383887625395033 1101 74-75 0.48434066440016466 1101 76 -0.8656174161667707 1102 1 -0.07437419008953583 1102 2 -0.19165670058826834 1102 3 -0.22767038664748185 1102 4 0.01390072285103372 1102 5 -0.15781869304975515 1102 6 0.007550470043540258 1102 7 -0.03270927259561063 1102 8 -0.03315497610610407 1102 9 -0.16396984942486625 1102 10-11 -0.1802201316738845 1102 12-13 -0.04172198656084447 1102 14-15 -0.03756924641630377 1102 16-17 -0.10295790053117315 1102 18-19 -0.10025346413324598 1102 20-21 -0.1541083094679223 1102 22-23 -6.637034863103963E-4 1102 24-25 -0.11344695518391745 1102 26-27 -0.12417730752255096 1102 28-29 -0.17755977807722445 1102 30-31 -0.11068338737756989 1102 32-33 -0.09476578427311466 1102 34-35 -0.2479658945201635 1102 36-37 -0.13490579351555 1102 38-39 -0.03660051218675875 1102 40-41 -0.2562180516282595 1102 42-43 -0.1656520169156792 1102 44-45 0.054188020856180685 1102 46-47 -0.031649210989840526 1102 48-49 -0.07756578477341236 1102 50-51 -0.2591165445504231 1102 52-53 0.011614068938889943 1102 54-55 -0.2935151055748477 1102 56-57 -0.13324324296617363 1102 58-59 -0.12142023174524752 1102 60-61 -0.06972872798466767 1102 62-63 -0.1550873465254412 1102 64-65 0.16539231895590234 1102 66-67 0.14835888752712378 1102 68-69 0.012875526549855465 1102 70-71 0.02455809176903756 1102 72-73 -0.3606621017735847 1102 74-75 -0.4068304725152174 1102 76 -0.24009458718668952 1103 1 -0.19332176779514754 1103 2 0.03363324573024329 1103 3 -0.19435472623261063 1103 4 -0.15135873768313246 1103 5 -0.011062778144854235 1103 6 -0.06894233928775861 1103 7 0.08501224639173444 1103 8 -0.04659523059612525 1103 9 -0.270597193847955 1103 10-11 -0.11966277200790643 1103 12-13 0.026652325852175807 1103 14-15 0.05372905035618203 1103 16-17 -0.103564302545891 1103 18-19 0.07966236859257947 1103 20-21 0.031732369172146946 1103 22-23 0.13094210206740797 1103 24-25 -0.0987342505646609 1103 26-27 0.049316628457297895 1103 28-29 0.14610315518541483 1103 30-31 0.0640332936483432 1103 32-33 -0.07199754250759582 1103 34-35 -0.25122654797632293 1103 36-37 -0.005937671042225645 1103 38-39 -0.023060847530381068 1103 40-41 0.07056304718543771 1103 42-43 0.07422802815026586 1103 44-45 0.14120836680355353 1103 46-47 0.015994768067741916 1103 48-49 0.002843785111934949 1103 50-51 -0.09233279479894918 1103 52-53 0.037861666526531224 1103 54-55 -0.07818937159645856 1103 56-57 -0.37036299907990156 1103 58-59 0.07698257824878851 1103 60-61 -0.1930868034038511 1103 62-63 -0.0017968700193691234 1103 64-65 -0.01565347599406408 1103 66-67 -0.4574201568098033 1103 68-69 -0.21604620451250867 1103 70-71 -0.47789733933971945 1103 72-73 -0.12461597545640046 1103 74-75 -0.15071343361270806 1103 76 0.27880659910795913 1104 1 0.07038491932926405 1104 2 -0.06425704962840939 1104 3 0.07443443116414272 1104 4 0.1450419783378365 1104 5 0.11579440124396712 1104 6 0.05863725411462184 1104 7 -0.04028503017136842 1104 8 -0.07789557582051998 1104 9 -0.06153185693452201 1104 10-11 -0.07748466179182145 1104 12-13 -0.1145444520534653 1104 14-15 0.05661870641039002 1104 16-17 -0.08389290502638858 1104 18-19 0.07115961958349715 1104 20-21 -0.070153578916333 1104 22-23 -0.17320059068288174 1104 24-25 -0.127071419724615 1104 26-27 -0.12555231413315937 1104 28-29 -0.23731968076906895 1104 30-31 -0.10579153693597476 1104 32-33 -0.11867635595855575 1104 34-35 -0.18639468504319012 1104 36-37 -0.2397632687918687 1104 38-39 0.010189376226172442 1104 40-41 0.02565830354652121 1104 42-43 0.10426574711203074 1104 44-45 0.010524950360647267 1104 46-47 -0.10799513092214852 1104 48-49 -0.05296109917396308 1104 50-51 -0.4606872251786953 1104 52-53 -0.38375321648075555 1104 54-55 -0.35174134704716664 1104 56-57 -0.3832961278358198 1104 58-59 -0.08195489777729392 1104 60-61 -0.2389537002201152 1104 62-63 -0.1352356885847783 1104 64-65 -0.24227730926346425 1104 66-67 -0.2704363951424966 1104 68-69 -0.260519417656603 1104 70-71 -0.2621704501951143 1104 72-73 0.3079150308087861 1104 74-75 0.17549804829498328 1104 76 0.3860754905540631 1105 1 -0.29059929467003087 1105 2 0.12334764048884495 1105 3 0.057709391826037404 1105 4 -0.21134856021350146 1105 5 0.02840036033234128 1105 6 -0.07506154290076239 1105 7 -0.2035635439523915 1105 8 -0.3033409846830182 1105 9 -0.0736886002470385 1105 10-11 0.08915028059688979 1105 12-13 -0.19506066950435752 1105 14-15 -0.06955509187395137 1105 16-17 -0.10514817125653053 1105 18-19 -0.006019203826483022 1105 20-21 -0.06384679245878999 1105 22-23 -0.1701169908656155 1105 24-25 -0.2109567554943652 1105 26-27 -0.21888513987804714 1105 28-29 -0.015973048618420194 1105 30-31 -0.07730313533199507 1105 32-33 -0.022888071099920637 1105 34-35 0.0515881584672897 1105 36-37 -0.13228141507885738 1105 38-39 -0.66878631028721 1105 40-41 -0.03583603684229075 1105 42-43 -0.17153878057691685 1105 44-45 -0.3116858511215099 1105 46-47 -0.3013419153493402 1105 48-49 -0.1875989348168261 1105 50-51 -0.42425506437467675 1105 52-53 -0.09625511993743174 1105 54-55 -0.4939676299769715 1105 56-57 -0.17027394622067504 1105 58-59 -0.3811021491858497 1105 60-61 -0.199342766764957 1105 62-63 -0.20789062995830676 1105 64-65 -0.49864119942132845 1105 66-67 -0.5286847074109176 1105 68-69 -0.5309561975155006 1105 70-71 -0.8075869265161089 1105 72-73 -0.7129405095191146 1105 74-75 -0.9521223770641427 1105 76 -1.1949736947664746 1106 1 -0.012922646121161563 1106 2 -0.2462298387440498 1106 3 -0.1798688052819557 1106 4 -0.2052466219507636 1106 5 -0.19653810751710665 1106 6 -0.027015188680678648 1106 7 -0.12199498688132593 1106 8 -0.12010336802831745 1106 9 0.009072142240910352 1106 10-11 0.11348111845967424 1106 12-13 0.12165684664783072 1106 14-15 0.007981827416372766 1106 16-17 -0.0815480276815066 1106 18-19 -0.05273236145133353 1106 20-21 -0.015358175906641236 1106 22-23 -0.16798259439346452 1106 24-25 0.016481455971124603 1106 26-27 -0.1783764741001832 1106 28-29 -0.08602355625865243 1106 30-31 0.07998201490899959 1106 32-33 0.03630457580808155 1106 34-35 -0.1326557652328475 1106 36-37 -0.025619277862169554 1106 38-39 -0.009033464425243665 1106 40-41 -0.24870522974558185 1106 42-43 0.10564432884775243 1106 44-45 0.06659586357442038 1106 46-47 -0.04446441560571657 1106 48-49 0.04824226095796291 1106 50-51 -0.0703811027297192 1106 52-53 0.027347587477187574 1106 54-55 0.17048345196334225 1106 56-57 0.11143434010894282 1106 58-59 0.05355839845028498 1106 60-61 -0.08341875897089324 1106 62-63 0.2189309780818931 1106 64-65 0.09590038605708884 1106 66-67 -0.01771788349541481 1106 68-69 0.12281649246501303 1106 70-71 0.03413443539548666 1106 72-73 -0.07703137199476373 1106 74-75 0.07622727938135654 1106 76 0.06085904033761835 1107 1 0.0846491835935268 1107 2 0.11919913382777736 1107 3 -0.029032672302960805 1107 4 0.051334634630492815 1107 5 -0.053261017811450984 1107 6 0.10368229915966509 1107 7 0.06434232445598553 1107 8 0.04249754457259769 1107 9 0.2146079192052568 1107 10-11 -0.019096728403887653 1107 12-13 -0.012578850087862747 1107 14-15 0.15872080851249848 1107 16-17 0.18016660903312243 1107 18-19 -0.06261051418663044 1107 20-21 0.14804188927913486 1107 22-23 -0.045436462918758025 1107 24-25 0.039697622044428726 1107 26-27 0.2397903012094531 1107 28-29 0.05021785676846946 1107 30-31 -0.031808462952902516 1107 32-33 0.08446997218776886 1107 34-35 0.03889879025027909 1107 36-37 0.3008455218169175 1107 38-39 0.14034225637904996 1107 40-41 0.11260889049710698 1107 42-43 0.045331813178101754 1107 44-45 0.15838872322441944 1107 46-47 0.1297539818269584 1107 48-49 0.06995727374440719 1107 50-51 0.186732922241454 1107 52-53 0.1963041135765735 1107 54-55 0.26476151945569626 1107 56-57 0.08059958605990403 1107 58-59 0.10088703506463759 1107 60-61 -0.19175877802518926 1107 62-63 -0.10646828481736748 1107 64-65 0.2511365591504031 1107 66-67 0.0058398811337809775 1107 68-69 0.08410930197211286 1107 70-71 0.09122840320374337 1107 72-73 0.16626201415576247 1107 74-75 0.18013905293598498 1107 76 0.1307519852305603 1108 1 -0.08033512748650651 1108 2 -0.005482910249732242 1108 3 0.009375784241512974 1108 4 -0.01848879539444681 1108 5 0.022868924817231573 1108 6 -0.1847029489233165 1108 7 0.0944947576310895 1108 8 0.1201809068907167 1108 9 -0.004653247159183138 1108 10-11 -0.0027734438186399757 1108 12-13 -0.03840522604018304 1108 14-15 -0.019851283157834132 1108 16-17 0.025100047165558692 1108 18-19 0.08285083908328517 1108 20-21 0.1332596465371978 1108 22-23 0.04434005506934824 1108 24-25 0.0852883238064166 1108 26-27 0.10096347346574674 1108 28-29 0.17463996607722976 1108 30-31 0.14211574749523237 1108 32-33 -0.02658096883798322 1108 34-35 0.15141031711949182 1108 36-37 -0.01775095421029249 1108 38-39 0.17299501447261179 1108 40-41 -0.21675321720253038 1108 42-43 0.18075163877424671 1108 44-45 0.030373944076139026 1108 46-47 0.11498721139268753 1108 48-49 0.12451263056676964 1108 50-51 0.07079892091702078 1108 52-53 -0.012312105115206862 1108 54-55 7.69911635380538E-4 1108 56-57 0.20962934859092286 1108 58-59 0.22212494345619405 1108 60-61 0.255712737456399 1108 62-63 -0.08664206977704936 1108 64-65 0.09899116896974647 1108 66-67 -0.05920570028460759 1108 68-69 -0.05036052393348456 1108 70-71 0.24717998106967798 1108 72-73 0.26723429396935927 1108 74-75 0.23306362853058715 1108 76 -0.10669223710031162 1109 1 -0.014178329741760365 1109 2 0.1421332785235805 1109 3 0.11402725054970375 1109 4 0.10674449573983935 1109 5 0.06634917174692845 1109 6 0.0017994459814758557 1109 7 -0.006802536844318752 1109 8 -0.06329359748797714 1109 9 -0.011811859027993421 1109 10-11 -0.25132801762999435 1109 12-13 0.06091480371667046 1109 14-15 0.050364454042153284 1109 16-17 0.039936058180813916 1109 18-19 0.05324733612717836 1109 20-21 0.07867930955386271 1109 22-23 0.06867411492238773 1109 24-25 0.003580958984763072 1109 26-27 -0.07702297933439439 1109 28-29 -0.05592019367010437 1109 30-31 0.07764908691915196 1109 32-33 0.21003170914847402 1109 34-35 0.12426371240277234 1109 36-37 0.21000644911256217 1109 38-39 0.20361325229253424 1109 40-41 0.3116006283489483 1109 42-43 -0.01140521117550719 1109 44-45 0.1379847818360247 1109 46-47 0.12065798050298326 1109 48-49 -0.02723754236232878 1109 50-51 0.0380174380337408 1109 52-53 0.09132136351610143 1109 54-55 -0.1288649143261793 1109 56-57 -0.08773690870146567 1109 58-59 0.11572134653107469 1109 60-61 0.04258578792558865 1109 62-63 -0.1971959649232886 1109 64-65 0.2115158032083997 1109 66-67 0.3031753701791047 1109 68-69 -0.03598783237372771 1109 70-71 0.5134222645167767 1109 72-73 0.22372468172206084 1109 74-75 0.18058780597540647 1109 76 0.591484502957897 1110 1 -0.03197112845825956 1110 2 -0.0050353914219769536 1110 3 0.0710246993280137 1110 4 -0.025406157846340705 1110 5 0.051791427342521956 1110 6 -0.02711878341806795 1110 7 -0.035247343661602315 1110 8 0.12953405039083066 1110 9 0.26293104620858543 1110 10-11 -0.0947961010575753 1110 12-13 0.16729680313804351 1110 14-15 -0.06973364963739215 1110 16-17 0.14823804918578531 1110 18-19 0.1389697000839334 1110 20-21 0.17093753527122146 1110 22-23 -0.13697539455921515 1110 24-25 -0.07532938003841139 1110 26-27 0.0182204387766447 1110 28-29 0.10000021873356957 1110 30-31 0.20807043301736172 1110 32-33 0.10562887913348362 1110 34-35 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0.22411801357832672 2115 58-59 0.021742071919675254 2115 60-61 0.11295539068898108 2115 62-63 0.31109764474856405 2115 64-65 0.029308549322387023 2115 66-67 0.044149463443361014 2115 68-69 -0.15143860957579847 2115 70-71 -0.40071590474056507 2115 72-73 -0.30367082777706855 2115 74-75 -0.40163326483633455 2115 76 0.11462094509952436 2116 1 0.03131777732094321 2116 2 0.0444773532236411 2116 3 0.03492165635724831 2116 4 -0.2258730543418963 2116 5 -0.029630435357340446 2116 6 0.09161408120909442 2116 7 -0.005971304681175127 2116 8 -0.029767233574531815 2116 9 -0.04489489912108979 2116 10-11 -0.0361150836575348 2116 12-13 -0.02229846274865821 2116 14-15 0.0991023665411106 2116 16-17 -0.14657858535912283 2116 18-19 -0.1002604636012876 2116 20-21 0.008426337898882252 2116 22-23 0.1356229387288792 2116 24-25 0.13696601343046666 2116 26-27 0.10469129846339342 2116 28-29 -0.03425966888552523 2116 30-31 0.14495118439497645 2116 32-33 -0.08354563700431328 2116 34-35 0.12005213610950705 2116 36-37 -0.11156956706875576 2116 38-39 -0.0631914912723417 2116 40-41 -0.04460313436197083 2116 42-43 -0.1671988993526199 2116 44-45 -0.3393710686530227 2116 46-47 -0.0706362479750311 2116 48-49 -0.006318199589884443 2116 50-51 0.19038229353788694 2116 52-53 0.01820756783296673 2116 54-55 0.18078984724873237 2116 56-57 0.024160794326988366 2116 58-59 0.02143191149186663 2116 60-61 -0.08097687312564261 2116 62-63 -0.03474549250633885 2116 64-65 -0.15441337581129488 2116 66-67 -0.21265267559407164 2116 68-69 -0.4744974331052063 2116 70-71 0.05823953197958076 2116 72-73 0.36755555368460335 2116 74-75 0.58652003284638 2116 76 0.07546942994800787 2117 1 -0.007661166529324248 2117 2 -0.022590382961737987 2117 3 -0.16791089711868779 2117 4 -0.018862693379332995 2117 5 -0.023015573503506914 2117 6 0.12445164805401276 2117 7 0.26676989407105367 2117 8 -0.37903193945688685 2117 9 -0.051036276126438906 2117 10-11 0.1753167902283863 2117 12-13 -0.05393839144741008 2117 14-15 0.10688701449120686 2117 16-17 0.0430799485089608 2117 18-19 0.09278209433097118 2117 20-21 -0.10807182386582781 2117 22-23 -0.09081422704652198 2117 24-25 -0.1301490712397566 2117 26-27 0.03038202930118672 2117 28-29 -0.3885586327892696 2117 30-31 -0.25430795107738646 2117 32-33 -0.22607850242319927 2117 34-35 -0.012704659790664152 2117 36-37 -0.05269729656964728 2117 38-39 -0.18279515660211842 2117 40-41 -0.1309862671605444 2117 42-43 0.07984182034435605 2117 44-45 0.14187753874448106 2117 46-47 -0.016296898598923804 2117 48-49 0.06687002456341418 2117 50-51 -0.04011430851202391 2117 52-53 -0.1755382543595445 2117 54-55 0.3608533499225288 2117 56-57 -0.16766323642167436 2117 58-59 -0.1278829280803251 2117 60-61 -0.1415341926443574 2117 62-63 -0.1248190219181069 2117 64-65 -0.3641602006776097 2117 66-67 9.098801100222431E-4 2117 68-69 -0.2688448595758004 2117 70-71 0.0020340952594324335 2117 72-73 -0.31889999444373984 2117 74-75 -0.34857076483633165 2117 76 -0.48489988823381225 2118 1 0.07400001768120035 2118 2 -0.12566055840033385 2118 3 -0.27490102869763433 2118 4 0.031219543462775334 2118 5 0.03227499667193712 2118 6 -0.1026865098407228 2118 7 0.14886967477281132 2118 8 0.08741542896416377 2118 9 0.132434118610405 2118 10-11 -0.03292060012249465 2118 12-13 0.0666756436403162 2118 14-15 -0.04275662585968121 2118 16-17 0.036309115175626516 2118 18-19 -0.024268344265522046 2118 20-21 -0.06510307386582781 2118 22-23 0.07727787821663412 2118 24-25 0.16527307788304313 2118 26-27 -0.05273200578653814 2118 28-29 0.11197590668441393 2118 30-31 0.1607688033085779 2118 32-33 0.18135022564697323 2118 34-35 -0.10675619487839327 2118 36-37 -0.14955859042930086 2118 38-39 -0.0059722399354456 2118 40-41 -0.14987332856406255 2118 42-43 0.10995421069522848 2118 44-45 0.14583861330589087 2118 46-47 0.16336318912037484 2118 48-49 0.09621487105464155 2118 50-51 0.09457593271604026 2118 52-53 -0.08478989909638557 2118 54-55 0.1623336130804276 2118 56-57 -0.023460933790097727 2118 58-59 -0.012257928080323666 2118 60-61 0.11599047840827836 2118 62-63 -0.019884811391790436 2118 64-65 -0.09816513488814138 2118 66-67 0.031570516074935995 2118 68-69 0.07256139042419818 2118 70-71 -0.15136502754758396 2118 72-73 0.1852940845036315 2118 74-75 0.3764018228829613 2118 76 0.09149813808197393 2119 1 0.07315067209501791 2119 2 0.01775353238217292 2119 3 0.07948493621464792 2119 4 0.03549411878997688 2119 5 -0.04017000472043719 2119 6 0.1747988702762342 2119 7 0.08104734116100332 2119 8 0.06111356318861283 2119 9 0.006048710646048505 2119 10-11 0.13842442911727204 2119 12-13 0.1643155768065654 2119 14-15 -0.32709942730774344 2119 16-17 0.043451972318493404 2119 18-19 0.026065824489705847 2119 20-21 0.15447364571089395 2119 22-23 0.16398074649845285 2119 24-25 0.06599014833695804 2119 26-27 0.034019595438742556 2119 28-29 0.11357017012078074 2119 30-31 0.10043741929298022 2119 32-33 -0.2076013198835227 2119 34-35 0.10869911005060118 2119 36-37 -0.17362570265430577 2119 38-39 0.18371029974709785 2119 40-41 0.166591444479657 2119 42-43 0.09218061002689382 2119 44-45 0.030787095622791583 2119 46-47 0.14615349822648227 2119 48-49 6.704544575910631E-4 2119 50-51 0.2059794414879761 2119 52-53 0.12730979458225278 2119 54-55 0.048932053626238314 2119 56-57 0.12716563262594605 2119 58-59 0.17081085498845994 2119 60-61 0.32483898857258 2119 62-63 0.2781240997750132 2119 64-65 -0.05631049829666068 2119 66-67 0.19264681794071237 2119 68-69 -0.23484601698320517 2119 70-71 0.35603541801076233 2119 72-73 0.3058106537044125 2119 74-75 0.5938482166451422 2119 76 -0.9993261448475721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 0.0 18 0.0 19 2.0 20 0.0 21 4.0 22 9.0 23 19.0 24 33.0 25 59.0 26 138.0 27 263.0 28 461.0 29 772.0 30 1193.0 31 1861.0 32 2935.0 33 4884.0 34 7826.0 35 14009.0 36 20555.0 37 92192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.48562656233018 14.38634387566569 11.493995217911095 35.63403434409304 2 21.509210955330943 17.49877730681448 34.556705792848604 26.435305945005975 3 19.73087164438648 16.7155743940876 32.373519182697535 31.180034778828386 4 15.188566460167374 22.687072057385066 30.778583849581565 31.345777632865996 5 15.863764808173025 28.410634713618084 38.50192913813716 17.223671340071732 6 29.53958808825128 36.879143571350944 16.09200086947071 17.48926747092707 7 28.36987827410064 24.216117813281166 20.34969025105967 27.064313661558526 8 24.557792631235735 36.765704814694054 14.768095859145744 23.908406694924462 9 22.82428540376046 15.837952396478643 15.435142919247907 45.90261928051299 10-11 26.367038908814262 25.87830127160091 23.222339963047496 24.53231985653733 12-13 25.389563634387567 18.157333442017173 25.61779969568525 30.83530322791001 14-15 22.67484512552983 17.395188023040976 19.92650255407021 40.00346429735898 16-17 28.39399250081513 24.386615585262472 18.505461362895335 28.71393055102706 18-19 30.541517226388436 22.762131833496362 19.132431257472014 27.56391968264319 20-21 25.131099880447778 20.654684273448538 19.155866210194546 35.05834963590914 22-23 23.446160743397456 25.374279969568526 19.19866047168786 31.98089881534616 24-25 28.478901749809804 25.670103793065973 18.926950874904904 26.924043582219326 26-27 29.188403434409306 28.303988696880772 19.092014454950547 23.415593413759375 28-29 25.024793500706444 26.45908053472449 20.604757635039668 27.911368329529402 30-31 23.717530703184437 26.736903597435063 19.415688512118248 30.129877187262256 32-33 22.420796652537767 31.546503097489403 20.598304532116074 25.434395717856756 34-35 27.951105858058906 23.3880828170851 25.093400173894143 23.567411150961853 36-37 30.439626127594828 22.80628464297359 23.47469025105967 23.279398978371916 38-39 23.554165308118684 22.899684816867733 26.63806923160526 26.908080643408326 40-41 22.868098576241714 18.563539289207696 27.809137593739813 30.75922454081078 42-43 27.589052820345618 18.402211716117815 26.89041951961743 27.118315943919143 44-45 22.109009890229324 23.82417671992175 29.823864253885446 24.24294913596348 46-47 25.60217639387023 20.002920878165416 27.166204760352137 27.228697967612213 48-49 25.38650690142376 18.93544179980437 23.62548907727421 32.052562221497666 50-51 22.690128790348876 19.68128464297359 27.745965112487774 29.88262145418976 52-53 19.07095696119987 19.677888273013803 35.055632539941314 26.195522225845018 54-55 19.895595587436148 20.47705412455168 30.254184327790455 29.373165960221716 56-57 23.581336267796978 21.52789099010977 24.975206499293556 29.915566242799695 58-59 20.33678404521248 21.61483806108032 30.71846810129334 27.32990979241387 60-61 23.87342408433866 25.519984240843385 25.212612759482667 25.393978915335293 62-63 19.81000706444952 33.93449081621563 20.796992174763613 25.458509944571244 64-65 18.954121834583198 35.500896641669385 19.929559287034017 25.615422236713403 66-67 18.980953157265514 35.38066514509293 19.35081784588632 26.28756385175524 68-69 18.83694707097055 35.887743180089124 19.31617487229649 25.95913487664384 70-71 18.874986414520162 34.95509998913162 19.524032713835453 26.645880882512767 72-73 19.052956200413 32.80349961960656 19.80898815346158 28.334556026518857 74-75 19.210547766547116 32.18671883490925 20.314028366481903 28.288705032061735 76 19.64732094337572 32.54129985871101 20.03586566677535 27.775513531137918 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.0 15 9.0 16 9.0 17 8.0 18 13.0 19 17.0 20 29.0 21 42.0 22 41.0 23 47.0 24 72.0 25 90.0 26 163.5 27 288.5 28 384.0 29 428.0 30 514.0 31 696.5 32 985.0 33 1177.0 34 1303.0 35 1667.0 36 2031.0 37 2157.0 38 2697.5 39 6042.5 40 10291.5 41 11050.5 42 10365.0 43 9323.0 44 7775.0 45 6889.0 46 6509.0 47 6388.5 48 5990.0 49 5477.5 50 5243.0 51 4955.5 52 4224.0 53 3613.0 54 3446.0 55 3380.0 56 3160.0 57 2916.0 58 2826.0 59 2824.5 60 2891.0 61 2978.0 62 2997.0 63 3085.0 64 3247.5 65 3346.0 66 3439.0 67 3508.0 68 3517.0 69 3526.5 70 3413.0 71 3299.0 72 3150.0 73 2731.5 74 2227.0 75 1992.0 76 1755.5 77 1256.5 78 776.0 79 558.0 80 396.5 81 150.0 82 38.5 83 12.0 84 8.5 85 4.5 86 2.5 87 1.0 88 0.5 89 0.5 90 1.5 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147216.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.86876426475383 #Duplication Level Percentage of deduplicated Percentage of total 1 61.15602385926666 27.439952179110964 2 21.614133890453267 19.395989566351485 3 8.261422472522481 11.120394522334529 4 3.3245526387501134 5.966742745353766 5 1.601719805008024 3.5933594174546246 6 1.000696399915221 2.6940006521030324 7 0.6328155751354951 1.9875557004673405 8 0.4905077663729676 1.7606781871535704 9 0.4117842976958246 1.6628627323117053 >10 1.4715232991189027 9.709542441039018 >50 0.016653041450934086 0.5828170850994457 >100 0.010597390014230781 1.0168731659602217 >500 0.0015139128591758258 0.5013042060645582 >1k 0.004541738577527478 5.343848494728834 >5k 0.0 0.0 >10k+ 0.0015139128591758258 7.224078904466906 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 10635 7.224078904466906 TruSeq Adapter, Index 5 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 2786 1.8924573415933053 RNA PCR Primer, Index 5 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 2670 1.8136615585262472 TruSeq Adapter, Index 5 (95% over 22bp) CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG 2411 1.6377295946092816 TruSeq Adapter, Index 5 (96% over 29bp) TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 738 0.5013042060645582 RNA PCR Primer, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 367 0.2492935550483643 TruSeq Adapter, Index 5 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 320 0.2173676774263667 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT 199 0.1351755243995218 No Hit CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 171 0.1161558526247147 TruSeq Adapter, Index 5 (95% over 22bp) TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 167 0.11343875665688512 No Hit CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 164 0.11140093468101292 TruSeq Adapter, Index 5 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005434191935659168 0.0 2 0.0 0.0 0.0 0.006113465927616564 0.0 3 0.0 0.0 0.0 0.007472013911531355 0.0 4 0.0 0.0 0.0 0.022416041734594067 0.0 5 0.0 0.0 0.0 0.026491685686338442 0.0 6 0.0 0.0 0.0 0.026491685686338442 0.0 7 0.0 0.0 0.0 0.026491685686338442 0.0 8 0.0 0.0 0.0 0.027170959678295838 0.0 9 0.0 0.0 0.0 0.03192587762199761 0.0 10 0.0 0.0 0.0 0.036001521573741986 0.0 11 0.0 0.0 0.0 0.03871861754157157 0.0 12 0.0 0.0 0.0 0.04007716552548636 0.0 13 0.0 0.0 0.0 0.04347353548527334 0.0 14 0.0 0.0 0.0 0.046190631453102926 0.0 15 0.0 0.0 0.0 0.04822845342897511 0.0 16 0.0 0.0 0.0 0.05094554939680469 0.0 17 0.0 0.0 0.0 0.052304097380719485 0.0 18 0.0 0.0 0.0 0.05773828931637866 0.0 19 0.0 0.0 0.0 0.06113465927616563 0.0 20 0.0 0.0 0.0 0.06453102923595261 0.0 21 0.0 0.0 0.0 0.06724812520378219 0.0 22 0.0 0.0 0.0 0.07064449516356917 0.0 23 0.0 0.0 0.0 0.08083360504293012 0.0 24 0.0 0.0 0.0 0.08490924899467449 0.0 25 0.0 0.0 0.0 0.09238126290620585 0.0 26 0.0 0.0 0.0 0.1161558526247147 0.0 27 0.0 0.0 0.0 0.20853711553092055 0.0 28 0.0 0.0 0.0 0.3117867623084447 0.0 29 0.0 0.0 0.0 0.42658406694924467 0.0 30 0.0 0.0 0.0 0.5583632213889794 0.0 31 0.0 0.0 0.0 0.6751983480056516 0.0 32 0.0 0.0 0.0 0.8327899141397674 0.0 33 0.0 0.0 0.0 1.037930659710901 0.0 34 0.0 0.0 0.0 1.3205086403651778 0.0 35 0.0 0.0 0.0 1.7219595696119987 0.0 36 0.0 0.0 0.0 2.283719160960765 0.0 37 0.0 0.0 0.0 3.038392566025432 0.0 38 0.0 0.0 0.0 3.8249918487120964 0.0 39 0.0 0.0 0.0 4.613628953374633 0.0 40 0.0 0.0 0.0 5.462042169329421 0.0 41 0.0 0.0 0.0 6.41438430605369 0.0 42 0.0 0.0 0.0 7.435333115965656 0.0 43 0.0 0.0 0.0 8.518775133137703 0.0 44 0.0 0.0 0.0 9.52342136724269 0.0 45 0.0 0.0 0.0 10.502934463645255 0.0 46 0.0 0.0 0.0 11.476334094120205 0.0 47 0.0 0.0 0.0 12.397429627214434 0.0 48 0.0 0.0 0.0 13.25874904901641 0.0 49 0.0 0.0 0.0 14.074557113357244 0.0 50 0.0 0.0 0.0 14.751793283338767 0.0 51 0.0 0.0 0.0 15.465030974894033 0.0 52 0.0 0.0 0.0 16.32159547875231 0.0 53 0.0 0.0 0.0 17.2161993261602 0.0 54 0.0 0.0 0.0 18.135936311270513 0.0 55 0.0 0.0 0.0 18.910987936093903 0.0 56 0.0 0.0 0.0 19.63984892946419 0.0 57 0.0 0.0 0.0 20.34697315509184 0.0 58 0.0 0.0 0.0 21.01945440712966 0.0 59 0.0 0.0 0.0 21.644386479730464 0.0 60 0.0 0.0 0.0 22.277469840234758 0.0 61 0.0 0.0 0.0 22.890854254972286 0.0 62 0.0 0.0 0.0 23.50559721769373 0.0 63 0.0 0.0 0.0 24.206607977393762 0.0 64 0.0 0.0 0.0 24.928676230844474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 85 0.0 61.7647 1 CTTTATA 90 0.0 58.33333 2 AGACTTT 20 0.0069400608 52.5 27 GTACACA 20 0.0069400608 52.5 2 TACCAAG 20 0.0069400608 52.5 21 ATCGTAT 20 0.0069400608 52.5 39 TAACACG 20 0.0069400608 52.5 4 ATCCACG 20 0.0069400608 52.5 2 AGCGACT 20 0.0069400608 52.5 67 CACCGTA 20 0.0069400608 52.5 26 TCAACGC 20 0.0069400608 52.5 3 AACACCA 20 0.0069400608 52.5 5 TGCCCAA 30 5.86262E-4 46.666668 34 TATGCAG 30 5.86262E-4 46.666668 10 TTTTGCC 30 5.86262E-4 46.666668 31 ACGAGTG 30 5.86262E-4 46.666668 5 TTTATAC 120 0.0 43.750004 3 TAATATG 35 0.0012522053 40.000004 18 ACTGAGC 35 0.0012522053 40.000004 8 CCGTCTT 2440 0.0 37.438522 47 >>END_MODULE