FastQCFastQC Report
Wed 25 May 2016
SRR1780307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555446
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA301.292144E-436.70554414
TAACCGC401.810748E-533.02009212
TTGTTCG401.8343486E-532.957679
CAGAGTA300.00575819829.31681428
GTTAACT300.0057785129.2957028
ATTGTTC508.406541E-526.3661338
GACTCAT450.001395244424.45050424
CTCCAAT551.6011296E-423.98000731
AACCGCT602.8513052E-422.0213413
AATCGAT500.002580381721.99752222
CTGTAGC703.2410408E-521.9737571
GCTCTAG500.002596631221.9737551
AGATGTA500.002596631221.9737552
ACAAAGT755.5009863E-520.51622841
TCGTTAC654.895422E-420.32738913
CTACGTA654.9703824E-420.281649
AGTGCGT550.00443231320.0374819
CGTCATA550.004483528419.99774723
ACAAGGA1605.4569682E-1119.2253063
TAATGCC708.072806E-418.87202839
Produced by FastQC (version 0.11.5)