FastQCFastQC Report
Wed 25 May 2016
SRR1780307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555446
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT231414.1662015749505805No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC63601.14502579908758No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG60031.0807531245161546No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG39330.7080796333036875TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC13060.23512636691955655No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT8860.15951145565905595No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT6080.10946158582472465TruSeq Adapter, Index 9 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT250.002352126435.19870439
TGGATAC250.002353165835.19553430
TCTTCTG5200.033.8449144
ATGCCGT30400.033.79480744
CGTCTTC5300.033.2063242
GTCTTCT5300.033.2063243
CCGTCTT5400.032.59139341
CTTTATA2550.031.9318922
TCTTTAT2600.030.5401551
TTAATAC651.7378625E-830.4713922
CCTTATA453.9947485E-529.4090371
TGCAAAA300.00574340729.33225323
CGGAAAA300.00574593529.3296112
GCGAAAG300.00574593529.3296131
AAACGTA300.00574593529.3296115
TATGCCG37200.027.61726243
CTTATAT655.789607E-727.1468051
GCCGTCT6600.026.66568640
TTTATAC3500.025.150993
GTTAATA450.001394633324.4523492
Produced by FastQC (version 0.11.5)