FastQCFastQC Report
Wed 25 May 2016
SRR1780306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85050
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGCA150.002217807870.0000125
TCTAACG150.002217807870.000018
GTCTGAG200.00693003552.512
TTATAGT200.00693003552.533
GACGCTA200.00693003552.514
ATGCACG200.00693003552.524
GGTATAT305.848579E-446.6666681
GTGAGCG404.5588404E-543.7511
GATGCAC350.001249224940.023
GTATAAG400.00240690235.01
CGATGCA501.6972268E-435.022
TTGATCT450.004286281331.11110939
GATGAAC450.004286281331.1111093
TAGATCT703.4533765E-530.039
GCCGTAT604.9460464E-429.16666854
CCGTATC755.528349E-527.99999855
GTATCAT500.00717347527.99999857
TGGTCGC500.00717347527.99999849
CGGTGGT657.898569E-426.92307746
CCCTGGG657.898569E-426.9230772
Produced by FastQC (version 0.11.5)