##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780306_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85050 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.37254556143445 34.0 27.0 34.0 12.0 34.0 2 29.585843621399178 34.0 31.0 34.0 12.0 34.0 3 29.404891240446798 34.0 27.0 34.0 11.0 34.0 4 29.418177542621986 34.0 27.0 34.0 11.0 34.0 5 29.237860082304525 34.0 27.0 34.0 11.0 34.0 6 32.17281599059377 37.0 31.0 38.0 11.0 38.0 7 32.45856554967666 37.0 31.0 38.0 12.0 38.0 8 32.47112286890064 38.0 31.0 38.0 11.0 38.0 9 32.59797766019988 38.0 32.0 38.0 12.0 38.0 10-11 32.33937683715462 38.0 31.0 38.0 11.0 38.0 12-13 32.43496178718401 38.0 31.0 38.0 11.0 38.0 14-15 32.25888300999412 38.0 31.0 38.0 11.0 38.0 16-17 32.305479129923576 38.0 31.0 38.0 11.0 38.0 18-19 32.35015873015873 38.0 31.0 38.0 11.0 38.0 20-21 32.353727219282774 38.0 31.0 38.0 11.0 38.0 22-23 32.31396237507349 38.0 31.0 38.0 11.0 38.0 24-25 32.34541446208112 38.0 31.0 38.0 11.0 38.0 26-27 32.311299235743675 38.0 31.0 38.0 11.0 38.0 28-29 32.343133450911225 38.0 31.0 38.0 11.0 38.0 30-31 32.35134626690182 38.0 31.0 38.0 11.0 38.0 32-33 32.40524985302763 38.0 31.0 38.0 11.0 38.0 34-35 32.392492651381545 38.0 31.0 38.0 11.0 38.0 36-37 32.289365079365076 38.0 31.0 38.0 11.0 38.0 38-39 32.25690182245738 38.0 31.0 38.0 11.0 38.0 40-41 32.3271722516167 38.0 31.0 38.0 11.0 38.0 42-43 32.42012933568489 38.0 31.0 38.0 11.0 38.0 44-45 32.48579071134627 38.0 31.5 38.0 11.0 38.0 46-47 32.3789594356261 38.0 31.5 38.0 11.0 38.0 48-49 32.32145796590241 38.0 31.0 38.0 11.0 38.0 50-51 32.25417401528513 38.0 31.0 38.0 11.0 38.0 52-53 32.32447971781305 38.0 31.0 38.0 11.0 38.0 54-55 32.304879482657256 38.0 31.0 38.0 11.0 38.0 56-57 32.259888300999414 38.0 31.0 38.0 11.0 38.0 58-59 32.30372721928278 38.0 31.0 38.0 11.0 38.0 60-61 32.339588477366256 38.0 31.0 38.0 11.0 38.0 62-63 32.198418577307464 38.0 31.0 38.0 11.0 38.0 64-65 32.16308054085832 38.0 31.0 38.0 11.0 38.0 66-67 32.10005291005291 38.0 30.5 38.0 11.0 38.0 68-69 32.0273721340388 37.5 30.5 38.0 11.0 38.0 70-71 32.062510288065845 37.5 30.5 38.0 11.0 38.0 72-73 32.014797178130515 38.0 31.0 38.0 11.0 38.0 74-75 32.00208700764256 38.0 30.0 38.0 11.0 38.0 76 30.344679600235157 36.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.26306829215209504 1101 2 -0.16330290906928013 1101 3 0.561518050468603 1101 4 0.9006026087373833 1101 5 0.14893694647246392 1101 6 0.5793853028091362 1101 7 0.5836846660250501 1101 8 -0.5308346809425117 1101 9 -0.026967103826244454 1101 10-11 -0.4191274553375166 1101 12-13 -0.6470820113131239 1101 14-15 -0.2665159810112847 1101 16-17 0.17327342898673237 1101 18-19 0.05175658680220607 1101 20-21 -0.1545937212080446 1101 22-23 -0.3357096367890904 1101 24-25 -0.5681655146230398 1101 26-27 -0.737492003524217 1101 28-29 -0.6648786116630774 1101 30-31 -0.5122540259282218 1101 32-33 -0.6286867393261701 1101 34-35 0.15025766522400374 1101 36-37 -0.008264072735670425 1101 38-39 -0.09641173371830547 1101 40-41 -0.029026661997292535 1101 42-43 -0.3261869997778106 1101 44-45 -0.3872986542322643 1101 46-47 0.1011164611639046 1101 48-49 -0.08890913939293199 1101 50-51 -0.14940783717747408 1101 52-53 0.2594590799141656 1101 54-55 0.1220074346928115 1101 56-57 -0.09504714923892266 1101 58-59 -0.07521150073242211 1101 60-61 0.07828604204350142 1101 62-63 -0.5619326762789711 1101 64-65 0.03415069279791183 1101 66-67 -0.026818956592979504 1101 68-69 -0.24921683950926976 1101 70-71 -0.07186276243655243 1101 72-73 -0.3747265260792503 1101 74-75 -0.6110221270638867 1101 76 -1.7391780147965008 1102 1 -0.4356346638335111 1102 2 -0.19727888884172273 1102 3 0.18142165350273487 1102 4 -0.34520965422089844 1102 5 -0.49103618879934174 1102 6 -0.13395224459920385 1102 7 -0.34417563738835 1102 8 -0.4901425191220312 1102 9 -0.022621338971624994 1102 10-11 -0.4239473036308148 1102 12-13 0.2977070215566613 1102 14-15 -0.07713940073315229 1102 16-17 -0.16832344206257233 1102 18-19 -0.18277644101068802 1102 20-21 -0.5198552572383832 1102 22-23 0.10627440240180874 1102 24-25 -0.471630274420761 1102 26-27 0.026335430104104773 1102 28-29 0.5035000229766169 1102 30-31 0.2580525480287932 1102 32-33 -0.33712542453476857 1102 34-35 0.6669493529610477 1102 36-37 0.5343836832693825 1102 38-39 -0.40201777038075903 1102 40-41 0.3726010244755287 1102 42-43 -0.06868067866529515 1102 44-45 -0.25204659987069533 1102 46-47 0.052029071783373126 1102 48-49 0.4701932626298273 1102 50-51 0.22509832590722567 1102 52-53 0.5432770318737141 1102 54-55 -0.12585918983310052 1102 56-57 -0.07578750954233726 1102 58-59 0.01012351823091251 1102 60-61 0.4034282670751139 1102 62-63 -0.10782000244837775 1102 64-65 0.2576612806613774 1102 66-67 0.00678170712383519 1102 68-69 0.5044770606171518 1102 70-71 0.21450338800592306 1102 72-73 0.2662145295465379 1102 74-75 0.2116509133912281 1102 76 0.01967154272562155 1103 1 0.16424448317830098 1103 2 0.1537990418432429 1103 3 0.08199083209226643 1103 4 0.21412526449572056 1103 5 0.281311867806636 1103 6 -0.10705270851874005 1103 7 -0.6169525271782206 1103 8 -0.04495519699034389 1103 9 -0.7209177016865311 1103 10-11 -0.2468099600574618 1103 12-13 -0.6546437639877638 1103 14-15 -0.18744699108050966 1103 16-17 0.39991951142852145 1103 18-19 0.3457799505530019 1103 20-21 0.06094081309025512 1103 22-23 -0.1393105650458537 1103 24-25 0.0033122229477626774 1103 26-27 -0.30715059383258847 1103 28-29 -0.1535113995800046 1103 30-31 -0.24844817319065626 1103 32-33 -0.2020819564438554 1103 34-35 -0.18054197291318275 1103 36-37 -0.4511821816091768 1103 38-39 -0.9514797796591523 1103 40-41 -0.07901879541454093 1103 42-43 -0.32488901362300027 1103 44-45 -0.356019154546928 1103 46-47 -0.32130919962904514 1103 48-49 -0.25064608086555396 1103 50-51 -0.608845769839526 1103 52-53 -0.1008304103312696 1103 54-55 -0.7822955860749659 1103 56-57 -0.08721006616781324 1103 58-59 -0.32993145038629734 1103 60-61 -0.3700698421603974 1103 62-63 0.20103735204072493 1103 64-65 -0.20584891387294846 1103 66-67 -0.48434924293659165 1103 68-69 -0.4970043631090171 1103 70-71 -0.16292829107091222 1103 72-73 -0.11799076459404034 1103 74-75 -0.2579993926397215 1103 76 -0.13455246413570876 1104 1 0.47831101819273414 1104 2 0.15258379043810777 1104 3 0.688211400222297 1104 4 1.011747928934426 1104 5 -0.22406182200044 1104 6 -0.006205830195789019 1104 7 0.34753934582800383 1104 8 0.28720719097866976 1104 9 0.20548363509001177 1104 10-11 0.3099365840713517 1104 12-13 0.4459752546967337 1104 14-15 0.12441690076211387 1104 16-17 0.2172235521394441 1104 18-19 0.010731340496789699 1104 20-21 -0.03259556849868517 1104 22-23 -0.2643580358038662 1104 24-25 0.126954559268583 1104 26-27 0.13535282406198945 1104 28-29 0.004148481932979564 1104 30-31 0.1940267622491092 1104 32-33 0.4298107976196377 1104 34-35 0.2691770001993703 1104 36-37 0.02860476962886338 1104 38-39 0.19984750273489738 1104 40-41 0.05317777150024483 1104 42-43 -0.057714093373874675 1104 44-45 -0.0025234079268443566 1104 46-47 0.14044835771563413 1104 48-49 -0.2486997797870174 1104 50-51 0.15470238449739782 1104 52-53 0.4321807547910126 1104 54-55 0.6087685184366549 1104 56-57 0.665343860613298 1104 58-59 -0.13278539235804487 1104 60-61 0.08659884992527367 1104 62-63 0.49417877692299683 1104 64-65 0.44386436274865915 1104 66-67 0.09117673306219487 1104 68-69 0.6315559437419616 1104 70-71 0.4340639617013835 1104 72-73 0.6464403458419312 1104 74-75 0.3488762719508891 1104 76 0.10995991623798318 1105 1 -0.8774180679368477 1105 2 -0.13935992380342554 1105 3 -0.44767077079981377 1105 4 -0.5645239127232209 1105 5 0.6974435127761147 1105 6 -0.13986517765210493 1105 7 4.437134215606875E-5 1105 8 -0.7285845207887647 1105 9 -0.28925890395436227 1105 10-11 0.3647050238424967 1105 12-13 -0.8634997723637312 1105 14-15 -0.20889066070378703 1105 16-17 -0.15475147492102082 1105 18-19 0.22329126008856548 1105 20-21 0.19305001678043254 1105 22-23 -0.2558629840024125 1105 24-25 0.3560116954986796 1105 26-27 0.40124118033228484 1105 28-29 -0.0663440184535915 1105 30-31 0.2016713424253922 1105 32-33 0.4208808455553168 1105 34-35 -0.009545746050818593 1105 36-37 0.14949737825728704 1105 38-39 0.07414199786401099 1105 40-41 0.38853419642678944 1105 42-43 -0.23918347639536108 1105 44-45 0.4399054617190501 1105 46-47 -0.5119020365939448 1105 48-49 -0.2634767242744154 1105 50-51 -0.33581678977196816 1105 52-53 -0.23413477018192452 1105 54-55 0.2621820022776902 1105 56-57 0.3761351858027169 1105 58-59 0.17414788427718264 1105 60-61 0.5547834795835485 1105 62-63 0.03867390604004939 1105 64-65 -0.39413045710599093 1105 66-67 0.1366479716581459 1105 68-69 -0.10534872612041468 1105 70-71 -0.49612004943206856 1105 72-73 -0.011167509423259503 1105 74-75 -0.38103133590437466 1105 76 0.0601493394635888 1106 1 -0.835755516821699 1106 2 -0.38805117842107606 1106 3 -0.6448814146037698 1106 4 -0.5479892729492128 1106 5 -0.2561330661140673 1106 6 -1.062999845082615 1106 7 -0.8063798399535536 1106 8 -0.370946386417657 1106 9 -0.5755432523473232 1106 10-11 -1.0794090790001931 1106 12-13 -0.6458331913005395 1106 14-15 -0.5002223214769828 1106 16-17 -0.6000804885714821 1106 18-19 -0.4780085957025122 1106 20-21 -0.1372970723722915 1106 22-23 -0.4300594637242696 1106 24-25 -0.2191547374046614 1106 26-27 -0.3864457480176071 1106 28-29 -0.8451413555271401 1106 30-31 -0.625100155569509 1106 32-33 -0.6514211634923157 1106 34-35 -0.7201895500056992 1106 36-37 -0.517261476763359 1106 38-39 -0.7752683259146593 1106 40-41 -0.9556707777933937 1106 42-43 -0.553963903490839 1106 44-45 -0.14897069639715 1106 46-47 -0.4798995079990931 1106 48-49 -0.4202496050946287 1106 50-51 -0.6308722015575867 1106 52-53 -0.26823129138854185 1106 54-55 -0.8505775244009541 1106 56-57 -0.463862048546666 1106 58-59 -0.6691384988444469 1106 60-61 -0.8744751285040095 1106 62-63 -0.9509450268139013 1106 64-65 -0.9833819535205244 1106 66-67 -0.7398558508661068 1106 68-69 -0.642378812448225 1106 70-71 -0.48671683732641924 1106 72-73 -0.4704136720830263 1106 74-75 -0.595003797926065 1106 76 -0.1477683231665452 1107 1 -0.33546648219734365 1107 2 -0.36231713014232625 1107 3 -0.43009878857470696 1107 4 -0.32188446301439555 1107 5 -0.5888418188798283 1107 6 0.09441439136764274 1107 7 0.640447755876373 1107 8 -0.016330802468047523 1107 9 0.4324301166003863 1107 10-11 -0.48073863827222496 1107 12-13 -0.04655478119030221 1107 14-15 -0.3318945362795418 1107 16-17 -0.16812322582904216 1107 18-19 -0.2796610764169216 1107 20-21 0.16021922320049242 1107 22-23 -0.1302009910230879 1107 24-25 0.043936537736371406 1107 26-27 0.006003471588904574 1107 28-29 0.43204284922897074 1107 30-31 0.09008113890693892 1107 32-33 0.0326739587992364 1107 34-35 0.008137836523296471 1107 36-37 -0.042008820634826805 1107 38-39 -0.46926039241255424 1107 40-41 -0.3487180000709884 1107 42-43 0.05483271580072824 1107 44-45 0.03758620355700515 1107 46-47 0.06668490007793082 1107 48-49 0.32835007198392674 1107 50-51 -0.09831095611735918 1107 52-53 -0.11707614956223722 1107 54-55 -0.019274077912228904 1107 56-57 0.4534640821767155 1107 58-59 0.29954609461340453 1107 60-61 0.24695928056968341 1107 62-63 0.2675053489311132 1107 64-65 0.4299873928625608 1107 66-67 0.004218939334624139 1107 68-69 0.27452871640540977 1107 70-71 0.11669775792543646 1107 72-73 0.5878118546576587 1107 74-75 0.02410836744160605 1107 76 -0.06610109171957745 1108 1 0.9708766619763445 1108 2 0.9377783491220697 1108 3 0.9638362025000582 1108 4 0.9999636834424948 1108 5 0.946651559840749 1108 6 1.2979305190607775 1108 7 1.2538190041639794 1108 8 0.9664621735791101 1108 9 0.555847444011242 1108 10-11 1.1004498710058712 1108 12-13 0.6760053084771727 1108 14-15 0.9334963060136232 1108 16-17 0.8595242481217298 1108 18-19 1.0999831595413951 1108 20-21 0.7658068358037511 1108 22-23 1.0465601862777518 1108 24-25 1.1384817658487805 1108 26-27 0.8089529506042297 1108 28-29 1.0499804971180495 1108 30-31 1.0713796569027068 1108 32-33 0.9622372449464365 1108 34-35 0.42862021437210274 1108 36-37 0.852216759505481 1108 38-39 0.9090969491126231 1108 40-41 1.1737092488808187 1108 42-43 0.8637704052025299 1108 44-45 0.4856330299092875 1108 46-47 1.1138744886868395 1108 48-49 0.7869099956005172 1108 50-51 0.43584363726551345 1108 52-53 0.7299702201500722 1108 54-55 0.4341797806954375 1108 56-57 1.2212003933560922 1108 58-59 1.4506731650604934 1108 60-61 1.1221097641929134 1108 62-63 0.649588457403862 1108 64-65 0.6463517085253052 1108 66-67 0.49712026359050654 1108 68-69 1.0443406637672688 1108 70-71 1.0329250816001405 1108 72-73 0.794732025690081 1108 74-75 1.4244259397775245 1108 76 0.7539412512585457 1109 1 -0.49552443250297173 1109 2 -0.2789044128286804 1109 3 -0.32797129790232304 1109 4 -0.5340778423904382 1109 5 -0.20750102345234822 1109 6 -0.5847750981933686 1109 7 -0.3347050582857243 1109 8 -0.8756028513685763 1109 9 -0.30343953490393716 1109 10-11 -0.43526154055054533 1109 12-13 -0.1907458584309154 1109 14-15 -0.836260655196746 1109 16-17 -0.12892738554960914 1109 18-19 -0.04258081921283008 1109 20-21 -0.12432273123310722 1109 22-23 -0.5910746117264303 1109 24-25 -0.07854302088870213 1109 26-27 -0.25832533685755266 1109 28-29 -1.2718648272019237 1109 30-31 -1.0772602915923883 1109 32-33 -0.6362055363186556 1109 34-35 -0.4570026731218597 1109 36-37 -0.3820501003045891 1109 38-39 -0.2241921221894856 1109 40-41 -0.9389577396915314 1109 42-43 -0.5086744684494917 1109 44-45 -0.5818866868137178 1109 46-47 -0.32953046866065705 1109 48-49 -0.30068858801448073 1109 50-51 -0.8609131629920128 1109 52-53 -0.7107790153239328 1109 54-55 -0.9044649963848812 1109 56-57 -0.3331815477351654 1109 58-59 -0.5507754105068621 1109 60-61 -0.8626600732447187 1109 62-63 -0.9796566737727055 1109 64-65 -1.2091528786306576 1109 66-67 -0.25406676361052405 1109 68-69 -0.8208412129428524 1109 70-71 -0.8528386396295353 1109 72-73 -0.7466328544000547 1109 74-75 -0.506424695986194 1109 76 -0.40145871655088783 1110 1 0.11531008622628391 1110 2 0.6325465117801414 1110 3 0.5790936617942144 1110 4 0.5877808584156305 1110 5 0.6112519400374588 1110 6 0.3596298330536669 1110 7 0.825560470400859 1110 8 0.13945167684384785 1110 9 1.1127529733938317 1110 10-11 0.038481939771877194 1110 12-13 0.8010156399095223 1110 14-15 1.094749302129003 1110 16-17 1.2867909643792643 1110 18-19 0.9616866061072287 1110 20-21 0.8186004551111381 1110 22-23 1.0837120170075636 1110 24-25 1.0069832949007775 1110 26-27 0.6853683568361433 1110 28-29 0.8911863819019175 1110 30-31 0.4986050474308499 1110 32-33 0.89882291318348 1110 34-35 1.3787607218520677 1110 36-37 1.1442656891690959 1110 38-39 0.45794396126238723 1110 40-41 1.2445415490709166 1110 42-43 1.272027285936474 1110 44-45 1.3646519570933506 1110 46-47 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-0.17499200352421695 2116 28-29 0.10515034586588001 2116 30-31 -0.10274630392049744 2116 32-33 0.335759850120418 2116 34-35 0.1580400011313472 2116 36-37 0.38300442790783507 2116 38-39 0.13136738468581655 2116 40-41 -0.7667197121903442 2116 42-43 -0.01626100235181127 2116 44-45 -0.011474169032780424 2116 46-47 0.281718938641383 2116 48-49 -0.2842115846953739 2116 50-51 -0.6860672644619044 2116 52-53 -0.8470402765851937 2116 54-55 -0.5198040196186398 2116 56-57 -0.8015907464075198 2116 58-59 0.28836636284543715 2116 60-61 0.10088900214645946 2116 62-63 0.18973640351510923 2116 64-65 -0.2699636894414681 2116 66-67 -0.07003404668307311 2116 68-69 -0.10658089999833109 2116 70-71 -0.32735262730141557 2116 72-73 -0.2704593124370369 2116 74-75 -0.1579775646443231 2116 76 -0.007196032814519526 2117 1 0.26846323937943595 2117 2 -0.02511114587751706 2117 3 0.2727873554879068 2117 4 0.623816894451668 2117 5 -0.04190121311569328 2117 6 0.45422442764826343 2117 7 0.9156505604909455 2117 8 0.06287510026727006 2117 9 0.7974376580785218 2117 10-11 0.8943377956277345 2117 12-13 1.1726372615311504 2117 14-15 0.6375420949217983 2117 16-17 0.8588630364513392 2117 18-19 0.5427676871883094 2117 20-21 -0.05527341876273795 2117 22-23 0.6535318368273835 2117 24-25 0.5317580696755471 2117 26-27 0.0367197081874977 2117 28-29 0.6907359314514707 2117 30-31 0.8454518942777014 2117 32-33 0.04296705732762973 2117 34-35 -0.12799603490469025 2117 36-37 0.11723866214207135 2117 38-39 0.3543403576587849 2117 40-41 0.2805775851069541 2117 42-43 0.49049575440494664 2117 44-45 0.31510240754379737 2117 46-47 0.7637009206233643 2117 48-49 0.5130857126019279 2117 50-51 0.6540228256281893 2117 52-53 0.13719395764903908 2117 54-55 -0.17295717277179534 2117 56-57 -0.5921312869480602 2117 58-59 0.36719519167426995 2117 60-61 0.50629440755187 2117 62-63 0.4037003674790718 2117 64-65 -0.3240177434955207 2117 66-67 0.33086685421782747 2117 68-69 -0.04577008918752057 2117 70-71 0.43165638170759024 2117 72-73 -1.020459312437037 2117 74-75 -0.3989685556353173 2117 76 -0.18737621299469964 2118 1 -0.5436112785774334 2118 2 -0.2627696188301094 2118 3 -0.8147141731900902 2118 4 -0.9553686259103245 2118 5 -1.3535477012327704 2118 6 -0.932734929899329 2118 7 -0.6219956830376496 2118 8 -0.10682854002460829 2118 9 -0.22820336756250725 2118 10-11 -0.8110553803536753 2118 12-13 -0.6347900753079507 2118 14-15 -0.2979578846958333 2118 16-17 -0.33101915343085153 2118 18-19 -0.6959506691771047 2118 20-21 -0.7560703695596906 2118 22-23 -0.6867620769959402 2118 24-25 -0.7479186658835388 2118 26-27 -0.7270282026192376 2118 28-29 -0.5159209516760015 2118 30-31 -0.7085613950297116 2118 32-33 -0.5238871271736159 2118 34-35 -0.7950295842911856 2118 36-37 -1.207167191675552 2118 38-39 -1.231703831326584 2118 40-41 -0.6971342896637438 2118 42-43 -0.8886061982264231 2118 44-45 -1.0506490905606434 2118 46-47 -1.435577543360985 2118 48-49 -0.9159938194187873 2118 50-51 -1.3426548475200732 2118 52-53 -1.1125512626844127 2118 54-55 -1.0961971548353056 2118 56-57 -0.5895223431626491 2118 58-59 -0.7479652176037916 2118 60-61 -0.8367511266701371 2118 62-63 -0.8071552845530512 2118 64-65 -1.1831347790831472 2118 66-67 -1.4324326443757833 2118 68-69 -1.2299961704724218 2118 70-71 -0.9579628755587244 2118 72-73 -0.9099257019034255 2118 74-75 -1.136525116721291 2118 76 -1.238046793077043 2119 1 -0.6291397207235221 2119 2 -0.5650886233549954 2119 3 -0.8268748066742546 2119 4 -0.4342188198340473 2119 5 -0.34213448209896313 2119 6 -0.7241861257622908 2119 7 -0.1439939054035193 2119 8 -0.26744182379965764 2119 9 0.38821146760233205 2119 10-11 -0.1401095981946554 2119 12-13 -0.08234986845597803 2119 14-15 -0.49196240958271176 2119 16-17 -0.28878014244184413 2119 18-19 -0.946011270340648 2119 20-21 -0.7617365783508987 2119 22-23 -0.8156203510748021 2119 24-25 -0.42307622890874796 2119 26-27 -0.6370455749527864 2119 28-29 -0.452825040234508 2119 30-31 -0.9564504544996524 2119 32-33 -0.7670795964690278 2119 34-35 -1.0595637633474233 2119 36-37 -0.9435319298785245 2119 38-39 -0.8798938765754514 2119 40-41 -0.6875088048544384 2119 42-43 -1.0069905712063836 2119 44-45 0.03904063148201686 2119 46-47 -1.202454967407526 2119 48-49 -1.0598912822500708 2119 50-51 -0.30342569432033173 2119 52-53 -0.6043802058001191 2119 54-55 -0.5677247081643273 2119 56-57 -1.1954935778103533 2119 58-59 -0.2292293578752833 2119 60-61 -0.665017529385068 2119 62-63 -0.6467541048503982 2119 64-65 -0.8832600214878035 2119 66-67 -1.1094082423072642 2119 68-69 -1.5036811252235545 2119 70-71 -1.1656127624365524 2119 72-73 -0.5890122403649656 2119 74-75 -0.9391471270638867 2119 76 -0.4579280147965008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 15.0 13 279.0 14 927.0 15 1739.0 16 2339.0 17 2694.0 18 2789.0 19 2442.0 20 1920.0 21 1321.0 22 943.0 23 747.0 24 645.0 25 670.0 26 682.0 27 737.0 28 882.0 29 1111.0 30 1279.0 31 1547.0 32 2002.0 33 2603.0 34 3543.0 35 5443.0 36 10045.0 37 35706.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.14756025867137 17.61199294532628 13.604938271604938 29.635508524397412 2 22.739564961787185 19.0652557319224 29.968253968253965 28.22692533803645 3 19.494415049970605 21.98941798941799 26.733686067019402 31.782480893592002 4 15.773074661963552 17.668430335097003 27.145208700764258 39.413286302175194 5 18.333921222810112 29.11228689006467 29.677836566725457 22.875955320399765 6 30.24808935920047 31.482657260435037 16.257495590828924 22.011757789535565 7 28.75602586713698 27.347442680776012 17.596707818930042 26.299823633156965 8 25.398001175778955 31.434450323339213 17.372134038800706 25.79541446208113 9 23.790711346266903 18.43503821281599 17.934156378600825 39.84009406231628 10-11 26.393298059964724 22.544385655496765 23.12169312169312 27.940623162845384 12-13 25.135214579659028 19.828336272780717 23.44914756025867 31.587301587301585 14-15 24.68665490887713 20.63256907701352 21.20576131687243 33.47501469723692 16-17 25.921810699588477 22.407995296884188 21.713697824808936 29.9564961787184 18-19 24.7419165196943 21.53909465020576 22.710758377425044 31.008230452674894 20-21 24.950029394473837 21.534391534391535 23.046443268665488 30.46913580246914 22-23 26.58847736625514 21.760141093474427 21.72310405643739 29.92827748383304 24-25 25.309229864785422 22.879482657260436 21.3098177542622 30.501469723691947 26-27 24.39858906525573 23.058201058201057 22.822457378012935 29.720752498530278 28-29 24.97178130511464 22.238095238095237 23.787184009406232 29.00293944738389 30-31 25.12933568489124 21.68371546149324 23.765432098765434 29.421516754850085 32-33 23.90358612580835 22.60435038212816 24.10111699000588 29.390946502057613 34-35 24.397413286302175 22.38447971781305 24.117577895355673 29.100529100529098 36-37 24.797766019988245 21.1928277483833 24.303938859494416 29.705467372134038 38-39 23.799529688418577 20.818342151675484 26.34920634920635 29.03292181069959 40-41 23.677248677248677 20.613756613756614 26.23691945914168 29.47207524985303 42-43 22.19400352733686 20.51851851851852 26.555555555555554 30.731922398589067 44-45 21.556731334509113 19.98824221046443 28.18048206937096 30.274544385655496 46-47 21.650205761316872 20.91416813639036 28.17871840094062 29.256907701352148 48-49 21.57319223985891 21.32686654908877 27.52028218694885 29.579659024103467 50-51 21.264550264550262 22.86360964138742 25.91710758377425 29.954732510288068 52-53 20.07995296884186 22.75132275132275 26.438565549676664 30.73015873015873 54-55 20.42151675485009 21.833039388594944 26.224573780129333 31.520870076425634 56-57 20.52498530276308 22.548500881834215 25.767783656672545 31.158730158730158 58-59 20.82422104644327 22.28453850676073 25.431510875955322 31.459729570840683 60-61 20.799529688418577 21.219870664315106 26.13521457965902 31.84538506760729 62-63 20.841857730746618 21.99882422104644 25.02527924750147 32.13403880070547 64-65 21.268665490887713 22.158142269253382 23.98177542621987 32.591416813639036 66-67 21.472663139329807 23.350970017636683 22.35626102292769 32.82010582010582 68-69 21.544973544973544 23.90005878894768 22.430335097001763 32.124632569077015 70-71 21.54673721340388 24.930041152263374 21.81716637272193 31.706055261610818 72-73 22.171075837742503 24.601998824221045 22.024103468547914 31.202821869488535 74-75 21.541572848752786 25.733837353541173 22.943697493812426 29.780892303893612 76 22.166960611405056 24.892416225749557 22.80893592004703 30.131687242798357 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 3.0 16 6.5 17 8.0 18 9.0 19 21.0 20 33.5 21 35.0 22 42.0 23 68.0 24 101.0 25 115.0 26 152.5 27 213.0 28 326.0 29 416.0 30 456.0 31 591.0 32 787.5 33 889.0 34 1031.5 35 1291.5 36 1582.5 37 1756.0 38 1936.5 39 2363.0 40 2770.5 41 3141.5 42 3351.0 43 3355.5 44 3508.0 45 3754.5 46 3853.0 47 3808.0 48 3704.0 49 3526.5 50 3408.0 51 3194.5 52 2829.0 53 2557.5 54 2438.0 55 2397.5 56 2224.5 57 2035.5 58 1979.0 59 1968.5 60 2004.0 61 2056.5 62 2063.0 63 2061.5 64 2088.5 65 2117.5 66 2117.0 67 2116.0 68 2222.0 69 2241.0 70 2117.5 71 2081.0 72 2086.5 73 1983.5 74 1766.0 75 1657.0 76 1458.5 77 1053.5 78 740.0 79 633.0 80 509.0 81 300.5 82 157.5 83 99.0 84 69.0 85 29.0 86 16.0 87 13.0 88 9.0 89 7.0 90 6.5 91 3.5 92 3.0 93 2.5 94 4.0 95 5.5 96 5.0 97 3.5 98 2.0 99 10.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.878894767783657E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 85050.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.20282186948853 #Duplication Level Percentage of deduplicated Percentage of total 1 81.47454348852439 62.90064667842445 2 12.54930628531396 19.376837154614933 3 3.6490458567490593 8.451499118165785 4 1.206195458491342 3.7248677248677247 5 0.5162882076118244 1.9929453262786598 6 0.2345380058177609 1.0864197530864197 7 0.15229740637516942 0.823045267489712 8 0.09290141788885335 0.5737801293356849 9 0.042643273785047446 0.2962962962962963 >10 0.08224059944259149 0.7736625514403292 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0011757789535567313 0.0 2 0.0 0.0 0.0 0.0011757789535567313 0.0 3 0.0 0.0 0.0 0.004703115814226925 0.0 4 0.0 0.0 0.0 0.010582010582010581 0.0 5 0.0 0.0 0.0 0.01763668430335097 0.0 6 0.0 0.0 0.0 0.01763668430335097 0.0 7 0.0 0.0 0.0 0.0188124632569077 0.0 8 0.0 0.0 0.0 0.0188124632569077 0.0 9 0.0 0.0 0.0 0.019988242210464434 0.0 10 0.0 0.0 0.0 0.019988242210464434 0.0 11 0.0 0.0 0.0 0.021164021164021163 0.0 12 0.0 0.0 0.0 0.022339800117577895 0.0 13 0.0 0.0 0.0 0.023515579071134628 0.0 14 0.0 0.0 0.0 0.023515579071134628 0.0 15 0.0 0.0 0.0 0.02704291593180482 0.0 16 0.0 0.0 0.0 0.029394473838918283 0.0 17 0.0 0.0 0.0 0.03409758965314521 0.0 18 0.0 0.0 0.0 0.04350382128159906 0.0 19 0.0 0.0 0.0 0.05291005291005291 0.0 20 0.0 0.0 0.0 0.05408583186360964 0.0 21 0.0 0.0 0.0 0.058788947677836566 0.0 22 0.0 0.0 0.0 0.06819517930629042 0.0 23 0.0 0.0 0.0 0.07289829512051735 0.0 24 0.0 0.0 0.0 0.07642563198118754 0.0 25 0.0 0.0 0.0 0.08465608465608465 0.0 26 0.0 0.0 0.0 0.09994121105232216 0.0 27 0.0 0.0 0.0 0.18106995884773663 0.0 28 0.0 0.0 0.0 0.27630805408583187 0.0 29 0.0 0.0 0.0 0.3985890652557319 0.0 30 0.0 0.0 0.0 0.5185185185185185 0.0 31 0.0 0.0 0.0 0.6643151087595532 0.0 32 0.0 0.0 0.0 0.8289241622574955 0.0 33 0.0 0.0 0.0 1.011169900058789 0.0 34 0.0 0.0 0.0 1.2768959435626102 0.0 35 0.0 0.0 0.0 1.6296296296296295 0.0 36 0.0 0.0 0.0 2.2045855379188715 0.0 37 0.0 0.0 0.0 2.9135802469135803 0.0 38 0.0 0.0 0.0 3.686067019400353 0.0 39 0.0 0.0 0.0 4.4373897707231045 0.0 40 0.0 0.0 0.0 5.202821869488536 0.0 41 0.0 0.0 0.0 6.161081716637272 0.0 42 0.0 0.0 0.0 7.214579659024103 0.0 43 0.0 0.0 0.0 8.23045267489712 0.0 44 0.0 0.0 0.0 9.223985890652557 0.0 45 0.0 0.0 0.0 10.119929453262786 0.0 46 0.0 0.0 0.0 11.04291593180482 0.0 47 0.0 0.0 0.0 12.023515579071134 0.0 48 0.0 0.0 0.0 12.807760141093475 0.0 49 0.0 0.0 0.0 13.532039976484421 0.0 50 0.0 0.0 0.0 14.223398001175779 0.0 51 0.0 0.0 0.0 15.038212815990594 0.0 52 0.0 0.0 0.0 15.822457378012933 0.0 53 0.0 0.0 0.0 16.731334509112287 0.0 54 0.0 0.0 0.0 17.570840681951793 0.0 55 0.0 0.0 0.0 18.353909465020575 0.0 56 0.0 0.0 0.0 19.05467372134039 0.0 57 0.0 0.0 0.0 19.789535567313344 0.0 58 0.0 0.0 0.0 20.46443268665491 0.0 59 0.0 0.0 0.0 21.112286890064667 0.0 60 0.0 0.0 0.0 21.72721928277484 0.0 61 0.0 0.0 0.0 22.29041740152851 0.0 62 0.0 0.0 0.0 22.88888888888889 0.0 63 0.0 0.0 0.0 23.48148148148148 0.0 64 0.0 0.0 0.0 24.117577895355673 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGGCA 15 0.0022178078 70.00001 25 TCTAACG 15 0.0022178078 70.00001 8 GTCTGAG 20 0.006930035 52.5 12 TTATAGT 20 0.006930035 52.5 33 GACGCTA 20 0.006930035 52.5 14 ATGCACG 20 0.006930035 52.5 24 GGTATAT 30 5.848579E-4 46.666668 1 GTGAGCG 40 4.5588404E-5 43.75 11 GATGCAC 35 0.0012492249 40.0 23 GTATAAG 40 0.002406902 35.0 1 CGATGCA 50 1.6972268E-4 35.0 22 TTGATCT 45 0.0042862813 31.111109 39 GATGAAC 45 0.0042862813 31.111109 3 TAGATCT 70 3.4533765E-5 30.0 39 GCCGTAT 60 4.9460464E-4 29.166668 54 CCGTATC 75 5.528349E-5 27.999998 55 GTATCAT 50 0.007173475 27.999998 57 TGGTCGC 50 0.007173475 27.999998 49 CGGTGGT 65 7.898569E-4 26.923077 46 CCCTGGG 65 7.898569E-4 26.923077 2 >>END_MODULE