FastQCFastQC Report
Wed 25 May 2016
SRR1780306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85050
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT85029.99647266313933No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG22812.681951793062904No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC19972.3480305702527926No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG16791.9741328630217518RNA PCR Primer, Index 15 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5220.6137566137566138No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT2970.3492063492063492RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT2060.24221046443268665No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1310.1540270429159318No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1160.13639035861258084No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1160.13639035861258084No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT1010.11875367430922987No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT960.1128747795414462No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGC960.1128747795414462No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAC150.002217807870.0000138
CATAATT150.002217807870.0000161
AACAAGA200.00693003552.570
GAGTGGC200.00693003552.531
CTCCGCT200.00693003552.532
CATTCTA200.00693003552.535
GCTTATA200.00693003552.53
GAATTCT200.00693003552.533
AATATCG305.848579E-446.66666836
TCTTTAT751.4551915E-1146.6666641
CTTTATA802.910383E-1143.752
TTTATAC901.8189894E-1242.7777753
ATCGTAT350.001249224940.039
ATATCGT350.001249224940.037
GTCAATA350.001249224940.033
CTTAATA556.62638E-638.181821
TATGCCG17750.033.91549343
ATCTCGT16850.033.85756737
AATCTCG16650.033.84384536
GTATGCC17800.033.82022542