##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780306_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85050 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73185185185185 34.0 34.0 34.0 34.0 34.0 2 33.73455614344503 34.0 34.0 34.0 34.0 34.0 3 33.74299823633157 34.0 34.0 34.0 34.0 34.0 4 33.761105232216345 34.0 34.0 34.0 34.0 34.0 5 33.76400940623163 34.0 34.0 34.0 34.0 34.0 6 37.52621987066431 38.0 38.0 38.0 37.0 38.0 7 37.52886537330982 38.0 38.0 38.0 37.0 38.0 8 37.44114050558495 38.0 38.0 38.0 37.0 38.0 9 37.45526161081717 38.0 38.0 38.0 37.0 38.0 10-11 37.41269841269841 38.0 38.0 38.0 37.0 38.0 12-13 37.38999412110523 38.0 38.0 38.0 37.0 38.0 14-15 37.31432686654909 38.0 38.0 38.0 36.0 38.0 16-17 37.33246913580247 38.0 38.0 38.0 36.5 38.0 18-19 37.283556731334514 38.0 38.0 38.0 36.0 38.0 20-21 37.31243974132863 38.0 38.0 38.0 36.0 38.0 22-23 37.31215167548501 38.0 38.0 38.0 36.0 38.0 24-25 37.31691358024692 38.0 38.0 38.0 36.0 38.0 26-27 37.204955908289236 38.0 38.0 38.0 36.0 38.0 28-29 37.1949265138154 38.0 38.0 38.0 36.0 38.0 30-31 37.191751910640804 38.0 38.0 38.0 36.0 38.0 32-33 37.11191064079953 38.0 38.0 38.0 36.0 38.0 34-35 37.08966490299824 38.0 38.0 38.0 36.0 38.0 36-37 37.052721928277485 38.0 38.0 38.0 36.0 38.0 38-39 36.29564373897708 38.0 37.0 38.0 34.0 38.0 40-41 36.710317460317455 38.0 37.0 38.0 34.5 38.0 42-43 37.026813639035865 38.0 38.0 38.0 36.0 38.0 44-45 37.11410346854791 38.0 38.0 38.0 36.0 38.0 46-47 37.063938859494414 38.0 38.0 38.0 36.0 38.0 48-49 37.07346266901823 38.0 38.0 38.0 36.0 38.0 50-51 36.91154027042916 38.0 38.0 38.0 35.0 38.0 52-53 36.89825396825397 38.0 38.0 38.0 35.0 38.0 54-55 36.87081716637272 38.0 38.0 38.0 35.5 38.0 56-57 36.926037624926515 38.0 38.0 38.0 35.5 38.0 58-59 36.775878894767786 38.0 37.5 38.0 34.5 38.0 60-61 36.77255731922399 38.0 37.0 38.0 35.0 38.0 62-63 36.76029394473839 38.0 37.0 38.0 35.0 38.0 64-65 36.65135802469136 38.0 37.5 38.0 34.5 38.0 66-67 36.279306290417395 38.0 37.0 38.0 34.0 38.0 68-69 36.12586713697824 38.0 37.0 38.0 34.0 38.0 70-71 35.1058201058201 38.0 36.5 38.0 27.0 38.0 72-73 33.39353909465021 38.0 34.5 38.0 16.0 38.0 74-75 32.9094532627866 38.0 34.0 38.0 11.0 38.0 76 32.26861845972957 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10380563804196186 1101 2 -0.062279934372384105 1101 3 -0.0898464945877464 1101 4 -0.06980762179897226 1101 5 -0.1811359796352363 1101 6 -0.05360310791840561 1101 7 -0.22164537577656773 1101 8 -0.4125175684326976 1101 9 0.04603227568068036 1101 10-11 -0.09138446274368306 1101 12-13 -0.19589107821579432 1101 14-15 0.10131846891025731 1101 16-17 0.08614449337731855 1101 18-19 0.1767056752385372 1101 20-21 -0.20647070794723277 1101 22-23 -0.20852674105795188 1101 24-25 -0.14640536921537972 1101 26-27 -0.2037077901810207 1101 28-29 -0.3079980949344119 1101 30-31 -0.5461178151846156 1101 32-33 -0.4095630297515669 1101 34-35 -0.3212829417901517 1101 36-37 -0.29904472499355705 1101 38-39 -0.3797988331278006 1101 40-41 0.021304477248420994 1101 42-43 -0.27901765741410145 1101 44-45 0.019328389940817203 1101 46-47 -0.2850562376869803 1101 48-49 0.17481716279506543 1101 50-51 -0.052415583156914636 1101 52-53 -0.2553079223278729 1101 54-55 -0.1717976559617327 1101 56-57 -0.26500996284254086 1101 58-59 0.10377985736089812 1101 60-61 -0.5346875440741954 1101 62-63 -0.2726137491693805 1101 64-65 -0.6148283574160942 1101 66-67 -0.3379969165789376 1101 68-69 -0.9340648271664662 1101 70-71 -0.6004101393545156 1101 72-73 -0.4409149253782658 1101 74-75 -0.4059935275506916 1101 76 -1.033893223951388 1102 1 0.01886345171784143 1102 2 0.042571519483495024 1102 3 -0.1308547120340151 1102 4 0.030144969857154535 1102 5 -0.07154369139250605 1102 6 0.16783336490081524 1102 7 -0.06954360586506425 1102 8 0.03410853776199474 1102 9 -0.1753251832320828 1102 10-11 0.009852104892210889 1102 12-13 -0.04056949161655865 1102 14-15 0.06874498597726131 1102 16-17 -0.12993878728007502 1102 18-19 0.1104130077290506 1102 20-21 -0.1812060113606364 1102 22-23 0.10229421343004219 1102 24-25 0.018141090961613315 1102 26-27 0.20979173573554277 1102 28-29 -0.17005956775363273 1102 30-31 -0.24890700608221294 1102 32-33 -0.21682835844941906 1102 34-35 0.1386372541643368 1102 36-37 -0.022594819810251465 1102 38-39 0.26465834133490773 1102 40-41 -0.21962867698672994 1102 42-43 0.05027279770600046 1102 44-45 0.11756242913171633 1102 46-47 -0.06865690140379144 1102 48-49 0.11946792132856388 1102 50-51 -0.026637296178407155 1102 52-53 -0.139216740279835 1102 54-55 0.03689782444281775 1102 56-57 -0.2973068971029704 1102 58-59 0.17115896608403602 1102 60-61 0.07369978209521122 1102 62-63 -0.08673353425155739 1102 64-65 0.013430966224866836 1102 66-67 0.01647922122131007 1102 68-69 0.24122752428738892 1102 70-71 0.0063376482561068315 1102 72-73 0.23311320357242948 1102 74-75 0.03990170000935933 1102 76 -0.35389954506390353 1103 1 0.09040062375791535 1103 2 0.10091232491018332 1103 3 0.016313039710553312 1103 4 0.013381490850491673 1103 5 0.022175851705227956 1103 6 -0.13480590842186757 1103 7 -0.10242731410255601 1103 8 -0.07372351556926304 1103 9 -0.18542225547098212 1103 10-11 0.076321248395395 1103 12-13 0.05699005771875676 1103 14-15 0.044964236688734616 1103 16-17 -0.0845701856661023 1103 18-19 0.16140517177723268 1103 20-21 0.00526820017108065 1103 22-23 0.03208252577653781 1103 24-25 0.25020806690796604 1103 26-27 0.17041705248732342 1103 28-29 0.40020085094349866 1103 30-31 0.24685339312249255 1103 32-33 0.10293500360275232 1103 34-35 0.07591065481148718 1103 36-37 0.2591778206326225 1103 38-39 0.22249624239140076 1103 40-41 0.46766421607787123 1103 42-43 0.12904165661988287 1103 44-45 0.16713164670608194 1103 46-47 0.014985061872486938 1103 48-49 0.10096961716887876 1103 50-51 0.04224890708852769 1103 52-53 0.25837009843990444 1103 54-55 0.11673876631643054 1103 56-57 -0.042316838235869625 1103 58-59 0.1867821386698978 1103 60-61 0.33422568751800696 1103 62-63 0.25167432509115883 1103 64-65 0.5282549496952953 1103 66-67 0.2266172275368561 1103 68-69 0.5061294774276135 1103 70-71 0.5127506535970312 1103 72-73 -0.08630904117435989 1103 74-75 -0.5848107868332519 1103 76 -0.6791890074630302 1104 1 -8.191848399903279E-4 1104 2 -0.17096220038223464 1104 3 0.039155968466445756 1104 4 0.03949040775767543 1104 5 0.11227535041402348 1104 6 0.20147694134268335 1104 7 0.004647727671709845 1104 8 -0.12049170636372963 1104 9 -0.014774374565618587 1104 10-11 0.06817526632035253 1104 12-13 -0.2178276545704776 1104 14-15 0.0498252423092751 1104 16-17 0.08468205495366732 1104 18-19 0.1813239018395194 1104 20-21 0.17430207530400565 1104 22-23 0.06510318505042534 1104 24-25 0.2275170445777306 1104 26-27 0.07346277632144194 1104 28-29 0.07215892477001518 1104 30-31 -0.028106731440772137 1104 32-33 -0.1908900001949121 1104 34-35 0.12599230451527177 1104 36-37 0.11436352623796608 1104 38-39 0.0365342949510179 1104 40-41 -0.19983160649542242 1104 42-43 -0.44850657367025804 1104 44-45 0.029257577132938195 1104 46-47 -0.030207099755948263 1104 48-49 0.06474942880491596 1104 50-51 -0.19550363734410325 1104 52-53 0.27678875254897406 1104 54-55 -0.28963940473020955 1104 56-57 -0.07404629289180065 1104 58-59 0.23486052238553157 1104 60-61 -0.1877817558968502 1104 62-63 -0.19271842897233427 1104 64-65 -0.250835130815112 1104 66-67 -0.5602105865296778 1104 68-69 0.05069502505028112 1104 70-71 -0.07239289797520598 1104 72-73 0.4419360598434068 1104 74-75 0.5017189108729596 1104 76 0.6933235248170888 1105 1 -0.00677248621620663 1105 2 0.11104645896521959 1105 3 0.05649399227964835 1105 4 0.09030608736181733 1105 5 -0.1021401436326741 1105 6 -0.011102307149663204 1105 7 -0.2381812502160301 1105 8 -0.10296039354480513 1105 9 0.07524031539881548 1105 10-11 -0.1012038893932683 1105 12-13 -0.09858766694097199 1105 14-15 0.06961803649513598 1105 16-17 -0.1317146514016727 1105 18-19 -0.17311014795173207 1105 20-21 -0.07577524029829164 1105 22-23 0.14854604882033584 1105 24-25 -0.07395581764587433 1105 26-27 0.034070476955427864 1105 28-29 0.03690301012644426 1105 30-31 -0.08125154356385877 1105 32-33 0.1564503430863624 1105 34-35 -0.36039048503165816 1105 36-37 -0.21042965452591034 1105 38-39 -0.3038959663757197 1105 40-41 -0.033738363879066924 1105 42-43 0.06533291913939365 1105 44-45 -0.37461980032183106 1105 46-47 -0.20300747087083693 1105 48-49 0.025734043000056772 1105 50-51 -0.27736153126709695 1105 52-53 0.09281627690338468 1105 54-55 -0.12396173347615047 1105 56-57 0.009203281872387947 1105 58-59 0.044973403164874526 1105 60-61 0.11218345208211389 1105 62-63 -0.08877726614567649 1105 64-65 -0.39506738688438503 1105 66-67 -0.15663280703377325 1105 68-69 0.2954955507963817 1105 70-71 -0.055847359085454684 1105 72-73 -0.06497402211113013 1105 74-75 -0.45144716304076127 1105 76 -0.03765683701993083 1106 1 -0.14307955245353554 1106 2 -0.22507886451712977 1106 3 -0.032145110069180305 1106 4 -0.17604582192483775 1106 5 0.13230801029553874 1106 6 -0.0026473181134960555 1106 7 -0.053969164322822394 1106 8 -0.01204950675868588 1106 9 -0.07529009688067845 1106 10-11 0.23050627042182015 1106 12-13 0.14950107093461185 1106 14-15 0.09342238646847534 1106 16-17 0.18635492446605184 1106 18-19 0.044665083671510786 1106 20-21 0.09777921338693574 1106 22-23 0.10697239361795141 1106 24-25 0.2458027805643539 1106 26-27 0.07790603927146122 1106 28-29 0.15791010204481637 1106 30-31 -0.01966643066604945 1106 32-33 0.06549006968204196 1106 34-35 -0.06946379452772078 1106 36-37 -0.11306887540261812 1106 38-39 -0.057239440427991894 1106 40-41 0.07339108832456986 1106 42-43 0.1863103790868479 1106 44-45 0.23981887137568236 1106 46-47 0.08987492971390765 1106 48-49 0.22872292113363812 1106 50-51 0.13916520664799492 1106 52-53 0.10859036275708434 1106 54-55 0.26431585882744457 1106 56-57 -0.0070745474869653435 1106 58-59 0.32334601532188145 1106 60-61 0.481802780029021 1106 62-63 0.17678445724974523 1106 64-65 -0.04002698863069298 1106 66-67 0.0680269191668117 1106 68-69 -0.020302240634059388 1106 70-71 -0.38372511732808334 1106 72-73 -0.7603178517470539 1106 74-75 -1.142079509300217 1106 76 -1.3708189634101657 1107 1 0.058706482190750364 1107 2 -0.026282843228237596 1107 3 0.018306381947482464 1107 4 -0.09190684610408084 1107 5 -0.13204903716594174 1107 6 -0.08331787844199567 1107 7 -0.06355714048245176 1107 8 -0.004853541283374341 1107 9 0.08467561763284692 1107 10-11 -0.02382426558789774 1107 12-13 -0.26559251648340876 1107 14-15 -0.008218134192524928 1107 16-17 -0.06882278199437053 1107 18-19 -0.1220580610252 1107 20-21 -0.08232922443074386 1107 22-23 0.03754428350572425 1107 24-25 -0.008537722156553684 1107 26-27 -0.033691225862988006 1107 28-29 0.22475683396022106 1107 30-31 -0.2746448029027775 1107 32-33 0.20882134322840074 1107 34-35 -0.1432872242723704 1107 36-37 0.04612940234969187 1107 38-39 0.040015727311761395 1107 40-41 -0.011834259015316206 1107 42-43 0.09596214219805432 1107 44-45 0.04172051986972036 1107 46-47 0.06512758584243272 1107 48-49 0.25293206260113266 1107 50-51 0.39107383183985434 1107 52-53 0.12657030973417704 1107 54-55 0.2037780550919166 1107 56-57 0.026612936317121694 1107 58-59 -0.07649850398364322 1107 60-61 0.07959615017274047 1107 62-63 -0.07357932770849374 1107 64-65 0.2585657117500304 1107 66-67 0.16350801231569534 1107 68-69 0.18166319481155568 1107 70-71 0.36254234933326757 1107 72-73 0.5998292569100343 1107 74-75 0.6125201279114947 1107 76 0.33932914191803576 1108 1 -0.2559599630747229 1108 2 -0.21865280462139935 1108 3 -0.2604723137227367 1108 4 -0.01369687474786474 1108 5 -0.20546428894061108 1108 6 -0.3340339729249564 1108 7 0.0071390278931602325 1108 8 -0.0951437152216883 1108 9 -0.03158076494841566 1108 10-11 -0.2433954391526001 1108 12-13 0.06875095979206236 1108 14-15 0.1773956314266414 1108 16-17 -0.037688239257015255 1108 18-19 0.05260672373132991 1108 20-21 0.10486202468710104 1108 22-23 0.07020441883719997 1108 24-25 0.09642719959196455 1108 26-27 0.12494964756471916 1108 28-29 0.14144079759507378 1108 30-31 0.01806141155195462 1108 32-33 -0.08008645701237782 1108 34-35 -0.14158520260272667 1108 36-37 -0.07626048515083284 1108 38-39 0.17780683528635421 1108 40-41 0.27521584684212996 1108 42-43 0.258785422533478 1108 44-45 0.29179316713609893 1108 46-47 0.2552247256157685 1108 48-49 0.2758574642367364 1108 50-51 0.03025561605672067 1108 52-53 0.11987638304564996 1108 54-55 -0.09379552621075504 1108 56-57 0.10378508958210375 1108 58-59 0.029545748579771214 1108 60-61 0.13509866169251694 1108 62-63 0.07359365581096 1108 64-65 0.21104402135192402 1108 66-67 0.15358647136338988 1108 68-69 0.13314519904584898 1108 70-71 0.4001632551409031 1108 72-73 0.6210526368759872 1108 74-75 1.2255800307717166 1108 76 1.429245701343504 1109 1 0.14815238200816339 1109 2 0.13242557941207878 1109 3 0.08762844275561577 1109 4 -0.05586651904208395 1109 5 0.07559960933719623 1109 6 -0.3295272933820641 1109 7 0.06234585229361045 1109 8 -0.07096055088883446 1109 9 -0.011220230584982005 1109 10-11 -0.0836698637461879 1109 12-13 -0.11385003811554384 1109 14-15 -0.20887106743059292 1109 16-17 -0.08565218582067757 1109 18-19 -7.692621048249748E-4 1109 20-21 0.19668562037357162 1109 22-23 0.03046918626034767 1109 24-25 -0.17984835167151658 1109 26-27 -0.08352451950432993 1109 28-29 0.0838800378976643 1109 30-31 0.07598431514119852 1109 32-33 0.07120921335621233 1109 34-35 -0.31302009717862234 1109 36-37 -0.14573175506875202 1109 38-39 -0.3302609258596263 1109 40-41 -0.030896337287927622 1109 42-43 -0.11086289550934225 1109 44-45 0.18775727465260417 1109 46-47 0.20754244652354714 1109 48-49 -0.09280501765606175 1109 50-51 -0.11096038265566222 1109 52-53 -0.07121487721509112 1109 54-55 -0.4028048614755164 1109 56-57 -0.01982669216585009 1109 58-59 -0.19027559376691983 1109 60-61 -0.18604249394887518 1109 62-63 -0.36683367398141087 1109 64-65 -0.09520899651383985 1109 66-67 -0.15500036269423134 1109 68-69 -0.06681858656496331 1109 70-71 -0.3405417183018855 1109 72-73 -0.6814381083356551 1109 74-75 -0.7150082518614695 1109 76 -1.5016250535253235 1110 1 0.07311682013050103 1110 2 -0.05190348649593801 1110 3 0.010459168217920478 1110 4 -0.0499395394308948 1110 5 0.07216196116269913 1110 6 -0.14389429195959025 1110 7 0.01089966926847552 1110 8 0.07425044958532112 1110 9 0.07317995907836661 1110 10-11 0.012762094527865031 1110 12-13 -0.0409481888979073 1110 14-15 -0.12678239981561035 1110 16-17 0.16873377909160325 1110 18-19 -0.04634773531487468 1110 20-21 -0.0475059396074613 1110 22-23 -0.12855167670788603 1110 24-25 0.15229958573957703 1110 26-27 -0.07510820845643451 1110 28-29 0.23851493595361717 1110 30-31 -0.09643152174832181 1110 32-33 0.03161698955345571 1110 34-35 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2115 66-67 0.12007938212236269 2115 68-69 0.019425432573790147 2115 70-71 -0.15609195753633998 2115 72-73 -0.25982726304060577 2115 74-75 -0.574378267810431 2115 76 0.26902885397068843 2116 1 0.001044748058426137 2116 2 -0.16001159460404324 2116 3 -0.09314443538568185 2116 4 0.058168568677217536 2116 5 0.1307205197212582 2116 6 0.07232192425662731 2116 7 -0.08369492532611389 2116 8 0.146322521657396 2116 9 -0.05294616704775734 2116 10-11 0.18618551795129434 2116 12-13 0.09643919848948457 2116 14-15 0.06691129387808559 2116 16-17 -0.17586081550299326 2116 18-19 -0.057608996576135496 2116 20-21 0.02006162796010358 2116 22-23 -0.11278591094212231 2116 24-25 -0.1157184322784417 2116 26-27 -0.10663973998796905 2116 28-29 -0.027250829812153654 2116 30-31 0.1603252350084361 2116 32-33 -0.36928391134744487 2116 34-35 0.04834784971563977 2116 36-37 -0.015160394234229102 2116 38-39 -0.08541739812136484 2116 40-41 -0.24243023961129495 2116 42-43 -0.22009712974357143 2116 44-45 -0.1347296859922551 2116 46-47 -0.3949943007500494 2116 48-49 -0.2846020778741831 2116 50-51 -0.3759556410719682 2116 52-53 -0.5559232698182228 2116 54-55 -0.07119035222942927 2116 56-57 -0.17357254328011607 2116 58-59 0.01716869262473608 2116 60-61 -0.27340617341781837 2116 62-63 -0.256888202193835 2116 64-65 -0.2679211823839225 2116 66-67 -0.1943555073125296 2116 68-69 -0.5719750588266947 2116 70-71 -0.05818040962478932 2116 72-73 -0.5617723899857339 2116 74-75 -0.13469767812983946 2116 76 0.14323032817216585 2117 1 -0.030486783473101298 2117 2 0.04719561260316141 2117 3 -0.11116245340370057 2117 4 0.06717757768622334 2117 5 -0.26567587667513237 2117 6 -0.05830870637399954 2117 7 -0.029640871272064828 2117 8 -0.40773153239666016 2117 9 -0.05745067155226735 2117 10-11 0.01951885128462294 2117 12-13 0.02661937866965758 2117 14-15 0.0804248073916014 2117 16-17 0.0448599052177272 2117 18-19 -0.15896034792748992 2117 20-21 -0.2096681017696298 2117 22-23 0.09892580076959234 2117 24-25 -0.11121392777393879 2117 26-27 0.13209899875077014 2117 28-29 -0.8020256045869232 2117 30-31 0.09275766744087122 2117 32-33 -0.008923550987077533 2117 34-35 0.08888839025618722 2117 36-37 -0.04669192576575654 2117 38-39 0.2704384577344854 2117 40-41 -0.06900681618787274 2117 42-43 0.027650618004173566 2117 44-45 -0.24058554184811243 2117 46-47 -0.19454385029958843 2117 48-49 -0.1855029787750766 2117 50-51 0.02944976433343527 2117 52-53 0.020653306758354972 2117 54-55 0.09322406218498713 2117 56-57 0.05615718644961021 2117 58-59 -0.2981466226905809 2117 60-61 -0.2058386058502535 2117 62-63 0.30392260861697906 2117 64-65 -0.038191452654189106 2117 66-67 0.2966354836784646 2117 68-69 0.11496187811024328 2117 70-71 0.05443220298782592 2117 72-73 0.20849788028453986 2117 74-75 0.44413115069898623 2117 76 0.21980690474874365 2118 1 0.13562955911382346 2118 2 -0.22990275273049576 2118 3 0.03188104258097013 2118 4 -0.010458882303176154 2118 5 0.11229485423677232 2118 6 0.00717985911456509 2118 7 -0.03188293233765194 2118 8 0.07206953563969876 2118 9 0.08920050451067851 2118 10-11 0.04404903757951928 2118 12-13 0.07377399935369766 2118 14-15 0.0981167074364393 2118 16-17 0.12122246687440708 2118 18-19 -0.040610097224295316 2118 20-21 -0.12531564977011556 2118 22-23 0.0579062744559522 2118 24-25 0.07064779820543521 2118 26-27 0.25363909087909775 2118 28-29 0.26095592898284536 2118 30-31 0.0737714866899779 2118 32-33 0.0708122934546438 2118 34-35 -0.15007455458911068 2118 36-37 -0.11224163837429302 2118 38-39 -0.4305725079823546 2118 40-41 -0.11138177032753305 2118 42-43 -0.06467134196483926 2118 44-45 -0.019365452980963482 2118 46-47 0.09906423742614123 2118 48-49 0.13754744721651946 2118 50-51 0.00872089066338333 2118 52-53 0.04059745905544787 2118 54-55 0.07255633563489994 2118 56-57 -0.10460878313988786 2118 58-59 -0.04934918271667499 2118 60-61 -0.25750792222545016 2118 62-63 -0.023805572052381763 2118 64-65 -0.13736189005992827 2118 66-67 0.06848538788130298 2118 68-69 -0.19616485948709084 2118 70-71 -0.3727517683137975 2118 72-73 -0.4725689331352143 2118 74-75 -0.09562466846859508 2118 76 -0.5475486861362597 2119 1 -0.06362706661338535 2119 2 0.11182720848475469 2119 3 0.1690820768408301 2119 4 0.09537094962959713 2119 5 -0.1364931224923822 2119 6 0.1338968920815944 2119 7 0.22478319565200167 2119 8 0.2080181458530177 2119 9 0.02817513282354156 2119 10-11 0.23450839439916393 2119 12-13 0.13669820749849038 2119 14-15 -0.3205565310897427 2119 16-17 0.05266235052017265 2119 18-19 0.1365271038099607 2119 20-21 0.13058286348134374 2119 22-23 0.10397325894204812 2119 24-25 -0.09506608350109502 2119 26-27 0.1221850669618405 2119 28-29 0.06923404792272692 2119 30-31 -0.11754638661318495 2119 32-33 -0.2622416011801363 2119 34-35 0.1251456296384248 2119 36-37 0.04693741786358885 2119 38-39 -0.1744416902706547 2119 40-41 -0.1594990941801484 2119 42-43 -0.002231943128386149 2119 44-45 0.2626319613693937 2119 46-47 -0.008270523401264995 2119 48-49 -0.052861408633511076 2119 50-51 0.11276298827165476 2119 52-53 0.05942422052926588 2119 54-55 0.05588091546684382 2119 56-57 0.19481146572888974 2119 58-59 0.262262000218044 2119 60-61 0.19522317021151991 2119 62-63 0.3055112508306195 2119 64-65 -0.02554264313038601 2119 66-67 0.1820923691353471 2119 68-69 0.4721851728335338 2119 70-71 0.2924470035026232 2119 72-73 -0.5145756396639811 2119 74-75 -0.4015292418364069 2119 76 -1.5696075096656728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 4.0 22 3.0 23 14.0 24 27.0 25 43.0 26 82.0 27 170.0 28 274.0 29 514.0 30 774.0 31 1157.0 32 1869.0 33 3078.0 34 5094.0 35 9310.0 36 13135.0 37 49501.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.579659024103464 13.255731922398589 11.617871840094063 38.54673721340388 2 20.232804232804234 17.358024691358025 35.50970017636684 26.899470899470902 3 19.062904174015287 14.720752498530276 34.673721340388006 31.542621987066433 4 15.64373897707231 24.50440917107584 28.946502057613166 30.905349794238685 5 16.170487948265727 26.81716637272193 39.39447383891828 17.61787184009406 6 28.826572604350382 37.159318048206934 15.87419165196943 18.13991769547325 7 27.305114638447968 22.90652557319224 19.57201646090535 30.216343327454435 8 23.916519694297474 37.241622574955905 14.166960611405056 24.674897119341562 9 21.622574955908288 15.660199882422104 14.372721928277485 48.344503233392125 10-11 25.82716049382716 26.581422692533803 22.61787184009406 24.97354497354497 12-13 24.68665490887713 16.832451499118164 26.075249853027632 32.405643738977076 14-15 22.30511463844797 15.573192239858907 19.052322163433274 43.069370958259846 16-17 29.950029394473837 24.067607289829514 16.54967666078777 29.432686654908878 18-19 32.592592592592595 22.000587889476776 16.84597295708407 28.56084656084656 20-21 25.477954144620814 18.653145208700764 16.98530276308054 38.88359788359788 22-23 23.008818342151677 24.546149323927104 18.045267489711932 34.399764844209294 24-25 29.806584362139915 24.764256319811874 18.887125220458554 26.54203409758965 26-27 32.13639035861258 26.336860670194 17.545561434450324 23.981187536743093 28-29 24.207524985302765 20.470311581422692 24.569664902998237 30.752498530276306 30-31 22.486184597295708 19.070546737213405 33.60846560846561 24.83480305702528 32-33 27.45737801293357 17.48148148148148 24.009994121105233 31.051146384479715 34-35 25.64726631393298 17.39271017048795 26.250440917107582 30.709582598471485 36-37 23.186948853615522 29.35920047031158 20.97530864197531 26.47854203409759 38-39 21.400940623162846 23.523221634332746 26.72898295120517 28.346854791299236 40-41 22.365667254556143 16.825984714873606 27.99588477366255 32.812463256907705 42-43 29.23456790123457 16.335684891240447 26.884185773074666 27.54556143445032 44-45 21.977660199882422 23.448559670781894 30.362727807172252 24.211052322163436 46-47 27.260435038212815 18.458553791887127 25.887713109935333 28.393298059964728 48-49 26.8477366255144 16.743092298647856 21.720164609053498 34.68900646678424 50-51 23.26219870664315 17.23045267489712 27.864785420340976 31.642563198118758 52-53 18.73780129335685 17.590828924162256 37.105232216343325 26.566137566137566 54-55 19.221046443268666 18.56554967666079 31.073486184597293 31.13991769547325 56-57 25.151675485008816 19.695473251028805 23.793650793650794 31.35920047031158 58-59 20.55026455026455 20.091122868900648 31.947677836566722 27.41093474426808 60-61 25.20164609053498 25.544385655496765 24.455026455026456 24.798941798941797 62-63 19.653733098177543 36.34567901234568 19.142269253380366 24.858318636096413 64-65 18.4808935920047 38.275720164609055 18.22045855379189 25.022927689594354 66-67 18.423868312757204 37.92710170487948 17.80658436213992 25.842445620223398 68-69 18.217519106407995 38.13639035861258 18.11875367430923 25.527336860670196 70-71 18.342151675485006 36.085243974132865 18.90534979423868 26.667254556143444 72-73 18.639035861258083 32.047619047619044 20.696061140505584 28.617283950617285 74-75 19.01822457378013 30.149323927101708 21.878306878306876 28.954144620811288 76 19.174603174603174 30.05055849500294 21.85185185185185 28.922986478542036 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 1.5 16 4.0 17 5.0 18 8.0 19 18.5 20 23.5 21 21.0 22 28.0 23 42.5 24 54.0 25 58.0 26 84.0 27 141.0 28 195.0 29 218.0 30 272.0 31 357.5 32 478.0 33 567.0 34 644.0 35 820.5 36 996.0 37 1072.0 38 1318.5 39 3546.5 40 6609.5 41 7029.5 42 6368.0 43 5479.5 44 4137.0 45 3425.5 46 3168.0 47 3041.5 48 2813.0 49 2575.5 50 2440.0 51 2335.0 52 2039.0 53 1832.0 54 1816.0 55 1750.0 56 1639.0 57 1654.5 58 1715.0 59 1675.5 60 1678.5 61 1774.0 62 1827.0 63 1866.0 64 1935.5 65 1992.5 66 2118.0 67 2217.0 68 2220.0 69 2203.5 70 2165.0 71 2146.0 72 2113.0 73 2008.5 74 1735.5 75 1534.0 76 1393.5 77 1067.0 78 727.0 79 573.0 80 484.0 81 292.0 82 133.0 83 77.0 84 51.0 85 16.5 86 5.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 85050.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.69429747207525 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9958394464274 37.58377425044092 2 19.83270195108065 21.298059964726633 3 6.0787877460748465 9.791887125220459 4 2.003635009963431 4.303350970017637 5 0.9437887314691134 2.533803644914756 6 0.3788293516105722 1.220458553791887 7 0.22335603389756278 0.8395061728395061 8 0.16423237786585498 0.7054673721340388 9 0.1029189567959358 0.4973544973544974 >10 0.2321150940504084 1.7319223985890653 >50 0.0197078853439026 0.7430922986478542 >100 0.013138590229268398 1.1369782480893593 >500 0.0021897650382114 0.6137566137566138 >1k 0.006569295114634199 7.004115226337449 >5k 0.0021897650382114 9.99647266313933 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 8502 9.99647266313933 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 2281 2.681951793062904 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 1997 2.3480305702527926 No Hit CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG 1679 1.9741328630217518 RNA PCR Primer, Index 15 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 522 0.6137566137566138 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 297 0.3492063492063492 RNA PCR Primer, Index 48 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT 206 0.24221046443268665 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 131 0.1540270429159318 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 116 0.13639035861258084 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 116 0.13639035861258084 No Hit CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT 101 0.11875367430922987 No Hit CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT 96 0.1128747795414462 No Hit CAGCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGC 96 0.1128747795414462 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0011757789535567313 0.0 2 0.0 0.0 0.0 0.0011757789535567313 0.0 3 0.0 0.0 0.0 0.004703115814226925 0.0 4 0.0 0.0 0.0 0.010582010582010581 0.0 5 0.0 0.0 0.0 0.01763668430335097 0.0 6 0.0 0.0 0.0 0.01763668430335097 0.0 7 0.0 0.0 0.0 0.0188124632569077 0.0 8 0.0 0.0 0.0 0.0188124632569077 0.0 9 0.0 0.0 0.0 0.019988242210464434 0.0 10 0.0 0.0 0.0 0.019988242210464434 0.0 11 0.0 0.0 0.0 0.021164021164021163 0.0 12 0.0 0.0 0.0 0.022339800117577895 0.0 13 0.0 0.0 0.0 0.023515579071134628 0.0 14 0.0 0.0 0.0 0.023515579071134628 0.0 15 0.0 0.0 0.0 0.02704291593180482 0.0 16 0.0 0.0 0.0 0.029394473838918283 0.0 17 0.0 0.0 0.0 0.03527336860670194 0.0 18 0.0 0.0 0.0 0.042328042328042326 0.0 19 0.0 0.0 0.0 0.05055849500293945 0.0 20 0.0 0.0 0.0 0.05291005291005291 0.0 21 0.0 0.0 0.0 0.058788947677836566 0.0 22 0.0 0.0 0.0 0.06466784244562022 0.0 23 0.0 0.0 0.0 0.06937095825984715 0.0 24 0.0 0.0 0.0 0.07289829512051735 0.0 25 0.0 0.0 0.0 0.07995296884185774 0.0 26 0.0 0.0 0.0 0.09523809523809523 0.0 27 0.0 0.0 0.0 0.17871840094062316 0.0 28 0.0 0.0 0.0 0.27983539094650206 0.0 29 0.0 0.0 0.0 0.40329218106995884 0.0 30 0.0 0.0 0.0 0.5291005291005291 0.0 31 0.0 0.0 0.0 0.6772486772486772 0.0 32 0.0 0.0 0.0 0.8500881834215167 0.0 33 0.0 0.0 0.0 1.0382128159905937 0.0 34 0.0 0.0 0.0 1.308641975308642 0.0 35 0.0 0.0 0.0 1.657848324514991 0.0 36 0.0 0.0 0.0 2.246913580246914 0.0 37 0.0 0.0 0.0 2.9653145208700766 0.0 38 0.0 0.0 0.0 3.7436801881246327 0.0 39 0.0 0.0 0.0 4.504409171075838 0.0 40 0.0 0.0 0.0 5.272192827748383 0.0 41 0.0 0.0 0.0 6.226925338036449 0.0 42 0.0 0.0 0.0 7.281599059376837 0.0011757789535567313 43 0.0 0.0 0.0 8.290417401528513 0.0011757789535567313 44 0.0 0.0 0.0 9.298059964726631 0.0011757789535567313 45 0.0 0.0 0.0 10.197530864197532 0.0011757789535567313 46 0.0 0.0 0.0 11.115814226925337 0.0011757789535567313 47 0.0 0.0 0.0 12.088183421516755 0.0011757789535567313 48 0.0 0.0 0.0 12.883009994121105 0.0011757789535567313 49 0.0 0.0 0.0 13.610817166372723 0.0011757789535567313 50 0.0 0.0 0.0 14.289241622574956 0.0011757789535567313 51 0.0 0.0 0.0 15.107583774250442 0.0011757789535567313 52 0.0 0.0 0.0 15.89182833627278 0.0011757789535567313 53 0.0 0.0 0.0 16.80893592004703 0.0011757789535567313 54 0.0 0.0 0.0 17.646090534979425 0.0011757789535567313 55 0.0 0.0 0.0 18.43621399176955 0.0011757789535567313 56 0.0 0.0 0.0 19.145208700764258 0.0011757789535567313 57 0.0 0.0 0.0 19.88947677836567 0.0011757789535567313 58 0.0 0.0 0.0 20.559670781893004 0.0011757789535567313 59 0.0 0.0 0.0 21.222810111699 0.0011757789535567313 60 0.0 0.0 0.0 21.83539094650206 0.0011757789535567313 61 0.0 0.0 0.0 22.385655496766606 0.0011757789535567313 62 0.0 0.0 0.0 22.973544973544975 0.0011757789535567313 63 0.0 0.0 0.0 23.562610229276896 0.0011757789535567313 64 0.0 0.0 0.0 24.20811287477954 0.0011757789535567313 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCGAC 15 0.0022178078 70.00001 38 CATAATT 15 0.0022178078 70.00001 61 AACAAGA 20 0.006930035 52.5 70 GAGTGGC 20 0.006930035 52.5 31 CTCCGCT 20 0.006930035 52.5 32 CATTCTA 20 0.006930035 52.5 35 GCTTATA 20 0.006930035 52.5 3 GAATTCT 20 0.006930035 52.5 33 AATATCG 30 5.848579E-4 46.666668 36 TCTTTAT 75 1.4551915E-11 46.666664 1 CTTTATA 80 2.910383E-11 43.75 2 TTTATAC 90 1.8189894E-12 42.777775 3 ATCGTAT 35 0.0012492249 40.0 39 ATATCGT 35 0.0012492249 40.0 37 GTCAATA 35 0.0012492249 40.0 33 CTTAATA 55 6.62638E-6 38.18182 1 TATGCCG 1775 0.0 33.915493 43 ATCTCGT 1685 0.0 33.857567 37 AATCTCG 1665 0.0 33.843845 36 GTATGCC 1780 0.0 33.820225 42 >>END_MODULE